Article
Food Science & Technology
Jingjing Dong, Shen Wang, Xiaoyao Yin, Min Fang, Zhiyong Gong, Yongning Wu
Summary: This study investigated the antihypertensive effect of rice peptide (RP) and revealed its mechanism of action. The results demonstrated that RP could effectively reduce systolic blood pressure in hypertensive rats, potentially through regulating the renin-angiotensin system and nitric oxide release.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Clinical Neurology
Malik Nassan, Iyas Daghlas, Ignazio S. Piras, Emily Rogalski, Lianne M. Reus, Yolande Pijnenburg, Leah K. Cuddy, Richa Saxena, M-Marsel Mesulam, Matt Huentelman
Summary: This study used genetic approaches to find that angiotensin-converting enzyme (ACE) inhibition is associated with Alzheimer's disease and frontotemporal dementia, but not with Lewy body dementia or vascular dementia. These results provide evidence for further research on the neurocognitive effects of ACE inhibition.
ALZHEIMERS & DEMENTIA
(2023)
Article
Biochemistry & Molecular Biology
Lizelle Lubbe, Bryan Trevor Sewell, Jeremy D. Woodward, Edward D. Sturrock
Summary: This study presents the first cryo-EM structures of full-length, glycosylated, soluble sACE (sACE(S1211)), revealing both monomeric and dimeric forms of the enzyme. The structures provide insights into the intradomain hinging, cooperativity, and homodimerization mechanisms of sACE. The observation of open conformation has implications for the design of sACE modulators.
Article
Biochemistry & Molecular Biology
Kyle S. Gregory, Gyles E. Cozier, Sylva L. U. Schwager, Edward D. Sturrock, K. Ravi Acharya
Summary: This study reports the structural basis of domain-specific inhibition of angiotensin-1-converting enzyme (ACE) by lactotripeptides Val-Pro-Pro and Ile-Pro-Pro. The milk-derived lactotripeptides LPP and VPP have shown promising results in reducing hypertension by inhibiting the N- and C-domain of ACE. The resolution of two X-ray crystal structures and kinetic analysis provide important insights into the molecular interactions underlying this inhibition.
Article
Biochemistry & Molecular Biology
Gyles E. Cozier, Emma C. Newby, Sylva L. U. Schwager, R. Elwyn Isaac, Edward D. Sturrock, K. Ravi Acharya
Summary: This study investigates the two isoforms of human angiotensin I-converting enzyme (ACE) and their different substrate specificities and structural features. By studying the structural characteristics of each domain, more specific and potent ACE inhibitors can be designed to reduce side effects and potentially be used clinically.
Article
Food Science & Technology
Estefania Bueno-Gavila, Adela Abellan, Francisco Giron-Rodriguez, Jose Maria Cayuela, Luis Tejada
Summary: This study aimed to obtain chicken egg ovalbumin hydrolysates using aspartic proteinases present in extracts from the artichoke flower and evaluate their antioxidant, antimicrobial, and ACE inhibitory activity in vitro. The hydrolysis time and molecular weight significantly influenced the activity of the hydrolysates, with specific peptides showing antioxidant and ACE inhibitory activity.
Review
Oncology
Jianhui Du, Jianhua Lan, Hai Yang, Qiao Ying, Guohua Huang, Jian Mou, Jia Long, Zhenghua Qiao, Qiyi Hu
Summary: This meta-analysis reveals that the ACE I/D locus polymorphism is associated with the risk of prostate cancer. The D allele, DD+DI genotype, and DD at the ACE I/D locus are found to increase susceptibility to prostate cancer, suggesting their potential as diagnostic and screening molecular markers for prostate cancer patients.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Moaz M. Abdou, Dewen Dong, Paul M. O'Neill, Eric Amigues, Magdalini Matziari
Summary: In drug discovery, molecular modification of lead molecules is crucial for drug development. This study reports the synthesis of a novel RXPA380-proline hybrid, which showed lower inhibition of ACE compared to the parent compound RXPA380.
Article
Biochemistry & Molecular Biology
Filippo Confalonieri, Xhevat Lumi, Goran Petrovski
Summary: Epiretinal membrane (ERM) is a common condition affecting the macula. The current treatment approach is surgery, but this case report suggests that angiotensin receptor blockers (ARBs) may have antifibrotic activity on ERM formation and resolution. Further clinical studies are needed to explore this phenomenon.
Article
Pharmacology & Pharmacy
Taikang Yao, Zhenchao Wu, Zilu Wang, Liting Chen, Beibei Liu, Ming Lu, Ning Shen
Summary: Observational studies and meta-analyses have shown a positive correlation between the use of angiotensin-converting enzyme inhibitors (ACEIs) and lung cancer. The study used Mendelian randomization to verify the associations between ACE inhibition, ACEI-induced cough, and the risk of lung cancer. The results indicate a significant relationship between ACE inhibition and the development of lung cancer, as well as a higher risk of lung cancer for Europeans with ACEI-induced cough.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Mitra Rezaei, Hadiseh Mohammadpour, Mahya Eftekhari, Mihan Pourabdollah, Farinaz Nasr Azadani, Payam Tabarsi, Majid Marjani, Seyed Ali Ziai
Summary: This study aimed to investigate the correlation between angiotensin I converting enzyme (ACE) insertion/deletion polymorphism and the severity of symptoms in COVID-19 patients. The study found that the ACE D allele was associated with an increased risk of disease severity.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Fatih Aydin, Vedat Turkoglu, Zehra Bas
Summary: The study successfully purified ACE enzyme from sheep lungs in one step and determined that captopril, a specific ACE inhibitor, has a significant inhibitory effect with a very low IC50 value of 0.51 nM.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Pharmacology & Pharmacy
Steven D. Brooks, Rachel L. Smith, Aline S. Moreira, Hans C. Ackerman
Summary: Lisinopril increases ACE2 expression in tissues relevant to COVID-19, while adding losartan prevents the lisinopril-induced increase. A sex difference was observed with higher ACE2 levels in the kidneys of male mice.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Se Yeong Park, Eun Woo Jeong, Yun Sun Yang, Hyun-Joo Kim, Gwang-woong Go, Hyeon Gyu Lee
Summary: Finger millet ethanol extracts (FEs) exhibit lipid-lowering, antioxidant, renin-angiotensin system-controlling, and vascular remodeling-improving effects in spontaneously hypertensive rats (SHRs), significantly reducing systolic blood pressure.
Review
Pharmacology & Pharmacy
Wenyue Zheng, Erkang Tian, Zhen Liu, Changhan Zhou, Pei Yang, Keyue Tian, Wen Liao, Juan Li, Changyu Ren
Summary: Angiotensin-converting enzyme inhibitors are drugs used to treat cardiovascular and kidney diseases. Researchers have been focusing on improving the selectivity of these drugs and developing novel ACE inhibitors to reduce their adverse effects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gyles E. Cozier, Lizelle Lubbe, Edward D. Sturrock, K. Ravi Acharya
Summary: ACE is a key enzyme involved in various physiological functions and has multiple hydrolytic activities. It contains two homologous catalytic domains (N- and C-domains) and the study of its structures provides important insights into how ACE domains can accommodate various substrates and allow different peptidase activities.
Review
Biochemistry & Molecular Biology
Samuel L. Crine, K. Ravi Acharya
Summary: C-mannosylation is a rare form of glycosylation that enhances the structural and functional diversity of proteins by forming a carbon-carbon bond with a tryptophan residue. Despite being discovered in 1994, it is still poorly understood and further research is needed to explore its mechanisms and functions.
Article
Chemistry, Medicinal
Lauren B. Arendse, Gyles E. Cozier, Charles J. Eyermann, Gregory S. Basarab, Sylva L. Schwager, Kelly Chibale, K. Ravi Acharya, Edward D. Sturrock
Summary: This study investigates the structural requirements for potent dual cACE/NEP inhibition by synthesizing and analyzing the crystal structures of inhibitors with high resolution. The results highlight the importance of the interplay between the S-1' and S-2' subsites for the selectivity of ACE domain.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Food Science & Technology
Kyle S. Gregory, Otsile O. Mojanaga, Sai Man Liu, K. Ravi Acharya
Summary: Botulinum neurotoxins (BoNT) are highly toxic proteins that cause botulism by proteolysis of a SNARE protein. They consist of three domains, with the H-C domain responsible for neuronal specificity. Some BoNTs have been commercialized for treating various neuromuscular conditions.
Article
Chemistry, Multidisciplinary
Xiaoqian Su, Xihu Wu, Shuai Chen, Anu Maashaa Nedumaran, Meera Stephen, Kunqi Hou, Bertrand Czarny, Wei Lin Leong
Summary: This study reports a highly stretchable and autonomic self-healable conducting film that exhibits outstanding stretchability, high electrical conductivity, and the ability to repair mechanical and electrical breakdowns. The film is utilized in a tactile sensor and an organic electrochemical transistor array, improving the practicality and shelf life of wearable electronics.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Gyles E. Cozier, Emma C. Newby, Sylva L. U. Schwager, R. Elwyn Isaac, Edward D. Sturrock, K. Ravi Acharya
Summary: This study investigates the two isoforms of human angiotensin I-converting enzyme (ACE) and their different substrate specificities and structural features. By studying the structural characteristics of each domain, more specific and potent ACE inhibitors can be designed to reduce side effects and potentially be used clinically.
Article
Food Science & Technology
Kyle S. Gregory, Tejaswini B. Mahadeva, Sai Man Liu, K. Ravi Acharya
Summary: Botulinum neurotoxins are toxic substances that can cause botulism, but they can also be used to treat neuromuscular conditions. This study reveals the crystal structures of the cell binding domain of botulinum neurotoxin A2 and its interactions with the receptor ganglioside GD1a. The findings provide important insights into the binding mechanism between the toxin and its receptor.
Article
Biochemistry & Molecular Biology
Kyle S. Gregory, Anna R. Newell, Otsile O. Mojanaga, Sai Man Liu, K. Ravi Acharya
Summary: This study determined the crystal structure of botulinum neurotoxin A6 cell binding domain (H-C/A6) in complex with GD1a and presented a new crystal form of wild type H-C/A6 (crystal form II), revealing the degree of conformational flexibility exhibited by H-C/A6.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Kyle S. Gregory, K. Ravi Acharya
Summary: Botulinum neurotoxins (BoNTs) are highly potent neurotoxins that cause neuromuscular paralysis by cleaving SNARE complex proteins. They have high affinity and specificity for neuromuscular junctions. This review focuses on the structural analysis of the cell-binding domain of different BoNT subtypes, providing insights into their functions and potential therapeutic applications.
Article
Pharmacology & Pharmacy
Yub Raj Neupane, Harish K. Handral, Syed Abdullah Alkaff, Wei Heng Chng, Gopalakrishnan Venkatesan, Chenyuan Huang, Choon Keong Lee, Jiong-Wei Wang, Gopu Sriram, Rhonnie Austria Dienzo, Wen Feng Lu, Yusuf Ali, Bertrand Czarny, Giorgia Pastorin
Summary: This study found that biomimetic cell-derived nanovesicles (CDNs) can effectively promote wound healing and tissue regeneration by mimicking extracellular vesicles (EVs). The results showed that CDNs can enhance cell proliferation, cell migration, secretion of growth factors and extracellular matrix proteins, as well as angiogenesis, accelerating wound healing.
ACTA PHARMACEUTICA SINICA B
(2023)
Review
Cell Biology
Meredith Qikai Lian, Wei Heng Chng, Jeremy Liang, Hui Qing Yeo, Choon Keong Lee, Mona Belaid, Matteo Tollemeto, Matthias Gerhard Wacker, Bertrand Czarny, Giorgia Pastorin
Summary: This review provides an overview of plant-derived extracellular vesicles, analyzing the current challenges and highlighting their immense potential as a novel therapeutic approach for human diseases.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biotechnology & Applied Microbiology
Wen Jie Melvin Liew, Yee Shan Wong, Atul N. Parikh, Subbu S. Venkatraman, Ye Cao, Bertrand Czarny
Summary: Lipid-based nanoparticulate formulations are commonly used for encapsulation and sustained release of hydrophilic drugs, but they struggle with high initial burst release. Nanolipogel (NLG) shows promise as a system for encapsulating and delivering hydrophilic drugs while suppressing initial burst release. However, there is limited understanding of the drug release mechanism from NLGs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Arun J. Thirunavukarasu, Evelina Han, Anu Maashaa Nedumaran, Arthur C. Kurz, Jeremy Shuman, Nur Zahirah binte M. Yusoff, Yu-Chi Liu, Valencia Foo, Bertrand Czarny, Andri K. Riau, Jodhbir S. Mehta
Summary: This study demonstrates that electron-beam-irradiated corneas can serve as a viable source of tissue for lenticule implantation. The results show that fresh and E-beam-irradiated lenticules are similar in various aspects, but differences exist in terms of tissue compression and refraction, suggesting the need for thicker lenticules to achieve the same optical outcome.
ACTA BIOMATERIALIA
(2023)
Review
Biochemistry & Molecular Biology
Amelia Barclay, K. Ravi Acharya
Summary: Plastic pollution is a significant environmental concern, and finding enzymes capable of degrading plastics is a potential solution. However, challenges such as efficiency and scalability, as well as the diverse range of plastic waste, hinder their widespread application. Structural biology provides insights into the interactions between enzymes and plastics, which is crucial for harnessing their potential to address the plastic waste crisis.
Article
Materials Science, Multidisciplinary
Samyukta Ravishankar, Anu Maashaa Nedumaran, Archana Gautam, Kee Woei Ng, Bertrand Czarny, Sierin Lim
Summary: Nanoparticles, both organic and inorganic, are used as tools for diagnostic and therapeutic applications. However, there is limited understanding of the cellular fate and molecular responses of organic nanoparticles in macrophages. In this study, we investigated the intracellular fate and immune responses of a model organic nanoparticle, Archaeoglobus fulgidus ferritin, in murine macrophage cells.
NPG ASIA MATERIALS
(2023)