Review
Plant Sciences
Hongmiao Hu, Jiamu Du
Summary: This review focuses on the central role of histone methylation in regulating chromatin state and gene expression in Arabidopsis. It summarizes the current understanding of the dynamic regulation of histone methylation, with a particular emphasis on the histone methyltransferases and demethylases involved.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Review
Oncology
Qiao Li, Qingsan Zhu
Summary: The AlkB family has the ability to catalyze the demethylation of various substrates, regulating gene transcription and expression. Altered methylation of DNA, RNA, and histones is frequently observed in cancer. The AlkB homologs play important roles in cancer genesis, progression, metastasis, and invasion, and may serve as new targets for tumor diagnosis and treatment.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anand Chopra, William G. Willmore, Kyle K. Biggar
Summary: JmjC KDMs are a type of demethylase that not only are associated with histone demethylation, but also with non-histone demethylation. Recent findings have highlighted the importance of KDM3A in promoting cancerous phenotypes, which can provide insights into the mechanisms through which it exerts its oncogenic functions.
Article
Cell Biology
Baoyu Chen, Yuwen Zhu, Junliang Chen, Yifei Feng, Yong Xu
Summary: This study reveals that differential TCL expression in malignant colorectal cancer cells is associated with histone H3K9 methylation, and the lysine demethylase KDM4B is essential for TCL transcription. KDM4B interacts with the transcription factor ERG1 to activate TCL transcription by facilitating the assembly of pre-initiation complex on the TCL promoter, ultimately influencing migration and invasion of CRC cells. Additionally, upregulation of KDM4B in advanced stage CRC specimens suggests it may serve as a potential therapeutic target for CRC intervention.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Toshiro Iwagawa, Masaya Fukushima, Shigeru Takeuchi, Yuichi Kawamura, Yuko Aihara, Manabu Ozawa, Nayuta Yakushiji-Kaminatsui, Makoto Aihara, Haruhiko Koseki, Yutaka Suzuki, Sumiko Watanabe
Summary: This study investigated the role of histone H3K36 methylation in retinal development by analyzing the loss-of-function of H3K36me1/2 demethylases Fbxl10 and Fbxl11. The results showed that knockout of Fbxl10 in the developing retina did not result in developmental abnormalities, while knockout of Fbxl11 in the developing retina increased apoptosis, suppressed the proliferation of retinal progenitor cells, and resulted in microphthalmia. The study also found that Fbxl11 knockout altered gene splicing and the distribution of H3K36me2/3. Overall, Fbxl11 plays a pivotal role in the development of late-born retinal cell types and regulates H3K36 methylation during retinal development.
Review
Pharmacology & Pharmacy
Chang-Yun Li, Yan-Jun Liu, Fan Tao, Ru-Yi Chen, Jin-Jin Shi, Jian-Fei Lu, Guan -Jun Yang, Jiong Chen
Summary: This article discusses the important role of lysine-specific demethylase 7A (KDM7A) in biological regulation and disease development, as well as its structure, function, and potential drug treatments. KDM7A is not only crucial for normal development but also associated with various diseases.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shusei Mori, Satoyo Oya, Mayumi Takahashi, Kazuya Takashima, Soichi Inagaki, Tetsuji Kakutani
Summary: Based on studies of the plant genome, it has been found that the demethylation of H3K4me2 plays a negative regulatory role in transcriptionally active genes. A previous study identified a key demethylase LDL3, which interacts with RNA polymerase II and RNA transcription elongation factors to demethylate H3K4me2. These findings suggest that H3K4me2 demethylation serves as a chromatin record of transcriptional activity in plants, ensuring robust gene control.
Article
Biochemistry & Molecular Biology
Mina S. Khella, Philipp Schnee, Sara Weirich, Tan Bui, Alexander Brohm, Pavel Bashtrykov, Juergen Pleiss, Albert Jeltsch
Summary: Protein lysine methyltransferases (PKMTs) are important in gene expression regulation and cancer development. Somatic mutations in PKMTs are frequently observed in cancer cells. NSD1 mutations Y1971C, R2017Q, and R2017L are inactive in solid cancers, suggesting NSD1 acts as a tumor suppressor gene in these tumors, while NSD2 and NSD1 T1150A are hyperactive in leukemia, introducing up to three methyl groups in H3K36.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Plant Sciences
Xiaochun Ding, Xuncheng Liu, Guoxiang Jiang, Zhiwei Li, Yunbo Song, Dandan Zhang, Yueming Jiang, Xuewu Duan
Summary: The study reveals that SlJMJ7 acts as a critical negative regulator of fruit ripening in tomato, regulating the expression of ripening-related genes through the removal of H3K4me3 and DNA demethylation. These findings highlight the importance of the crosstalk between histone methylation and DNA methylation in plant developmental processes.
Review
Gastroenterology & Hepatology
Xing-Yu Liu, Chuan-Hao Guo, Zhi-Yuan Xi, Xin-Qi Xu, Qing-Yang Zhao, Li-Sha Li, Ying Wang
Summary: In pancreatic cancer research, histone methylation plays a crucial role, with writers and erasers potentially serving as therapeutic targets, while further research on reader domains is needed.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Inosha D. Gomes, Udana Ariyaratne, Mary Kay H. Pflum
Summary: HDAC6 is upregulated in various tumor cell lines and plays roles in cell signaling, protein degradation, and other cellular processes. The study utilized substrate trapping mutants to identify PRMT5 as a novel substrate of HDAC6, revealing potential crosstalk between acetylation and methylation. Substrate trapping is a powerful and unbiased approach to discover substrates of HDAC6.
ACS CHEMICAL BIOLOGY
(2021)
Review
Cell Biology
Hao Song, Juanli Chen, Jin Huang, Peng Sun, Yanming Liu, Li Xu, Chuanfei Wei, Xin Mu, Xianjie Lu, Wei Wang, Nan Zhang, Miwei Shang, Mei Mo, Wei Zhang, Hui Zhao, Fabin Han
Summary: Parkinson's disease (PD) is a common neurodegenerative disorder caused by genetic, epigenetic, and environmental factors. Recent advance in genomics and epigenetics have revealed epigenetic mechanisms in PD. These epigenetic modifications include DNA methylation, post-translational histone modifications, chromatin remodeling, and RNA-based mechanisms, which regulate cellular functions in almost all cells. Epigenetic alterations are involved in multiple aspects of neuronal development and neurodegeneration in PD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Jessica Connacher, Gabrielle A. Josling, Lindsey M. Orchard, Janette Reader, Manuel Llinas, Lyn-Marie Birkholtz
Summary: In this study, the researchers identified a stage-specific association between repressive histone modifications (H3K36me2&3) and transcriptional reprogramming in Plasmodium falciparum early sexual development. They found that H3K36me2&3 are associated with the repression of genes involved in asexual proliferation and sexual commitment, essential for the transition from early gametocyte differentiation to intermediate development. Additionally, the study showed that the transcription factor AP2-G, a master regulator of commitment, is actively repressed in stage II gametocytes.
EPIGENETICS & CHROMATIN
(2021)
Article
Biochemistry & Molecular Biology
Tianqi Wang, Yang Liu, Hailin Zhang, Zhen Fang, Rong Zhang, Wenqing Zhang, Yan Fan, Rong Xiang
Summary: Crystal complex structures of two KDM4D inhibitors, OWS and 10r, have been determined, offering new insights for rational design and optimization of KDM4D inhibitors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Haipeng Guan, Pei Wang, Pei Zhang, Chun Ruan, Yutian Ou, Bo Peng, Xiangdong Zheng, Jianlin Lei, Bing Li, Chuangye Yan, Haitao Li
Summary: The Rpd3S complex recognizes and deacetylates histones H3 and H4 at transcribed regions, guided by H3K36me3 modifications. Cryo-electron microscopy structures of Rpd3S in free and H3K36me3 nucleosome-bound states reveal a unique architecture. Multivalent recognition of H3K36me3 marks, nucleosomal DNA, and linker DNAs positions the catalytic center for deacetylation. Combinatorial readout of unmethylated H3K4 and H3K36me3 directs specific histone H3 deacetylation.
Article
Multidisciplinary Sciences
Tianlong Zhang, Rong Wang, Zhijing Wang, Xiangxiang Wang, Fang Wang, Jianping Ding
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Chen Zhong, Jinlong Shen, Huibing Zhang, Guangyi Li, Senlin Shen, Fang Wang, Kuan Hu, Longxing Cao, Yongning He, Jianping Ding
Article
Chemistry, Multidisciplinary
Chao Cheng, Tianlong Zhang, Chunxi Wang, Wenxian Lan, Jianping Ding, Chunyang Cao
CHINESE JOURNAL OF CHEMISTRY
(2018)
Letter
Cell Biology
Hanwen Zhu, Tianlong Zhang, Fang Wang, Jun Yang, Jianping Ding
Article
Multidisciplinary Sciences
Tianlong Zhang, Marie-Pierre Peli-Gulli, Zhen Zhang, Xin Tang, Jie Ye, Claudio De Virgilio, Jianping Ding
Article
Cell Biology
Wenjing Li, Yu Shi, Tianlong Zhang, Jie Ye, Jianping Ding
Article
Biochemistry & Molecular Biology
Pengkai Sun, Tengfei Ma, Tianlong Zhang, Hanwen Zhu, Jianyang Zhang, Yabing Liu, Jianping Ding
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Pengkai Sun, Yan Liu, Tengfei Ma, Jianping Ding
Article
Cell Biology
Jun Yang, Hanwen Zhu, Tianlong Zhang, Jianping Ding
Summary: The study presents the crystal structures of D-2-hydroxyglutarate dehydrogenase (D-2-HGDH) and analyzes its substrate specificity and catalytic reaction at a molecular level. Insights into the pathogenicity of disease-associated mutations are provided through structural and biochemical data.
Article
Multidisciplinary Sciences
Wenjing Li, Tianlong Zhang, Mingliang Sun, Yu Shi, Xiao-Jie Zhang, Guo-Liang Xu, Jianping Ding
Summary: This study elucidates the molecular mechanism of CMD1-catalyzed C-5-glyceryl-methylcytosine (5gmC) DNA modification. CMD1 exhibits high binding affinities for DNA and moderate substrate preference for DNA containing 5mCpG.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yifan Zhang, Ziyue Chen, Fang Wang, Honghua Sun, Xueliang Zhu, Jianping Ding, Tianlong Zhang
Summary: This study demonstrates that Rab9 utilizes Nde1 as an effector to interact with the dynein motor complex, thereby tethering the late endosomes to the dynein motor for their retrograde transport to the TGN.
Article
Multidisciplinary Sciences
Xin Tang, Yifan Zhang, Guanchao Wang, Chunxiao Zhang, Fang Wang, Jiawen Shi, Tianlong Zhang, Jianping Ding
Summary: The mTORC1 complex is a central regulator of cell growth and metabolism, and SAMTOR functions as a sensor for S-adenosylmethionine (SAM) to regulate mTORC1 activity. In this study, the crystal structures of SAMTOR in its apo form and bound to SAM were determined, revealing the mechanism of SAM sensing and its functional role in mTORC1 signaling.
Article
Biochemistry & Molecular Biology
Guanchao Wang, Xueqian Feng, Jianping Ding
Summary: In this study, the T95R mutation in the IRF4 gene was found to enhance the binding affinity and specificity of the IRF4 protein to DNA, with the highest preference for the noncanonical DNA sequence GATA.
Letter
Cell Biology
Yu Shi, Hui Yang, Jianping Ding
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Letter
Cell Biology
Xueyan Ma, Peixue Li, Peihao Chen, Jinhui Li, Hongling Huang, Chao Wang, Wenjing Li, Jianping Ding, Yun Zhao, Fa-Xing Yu, Xiangbing Qi, Lei Zhang
JOURNAL OF MOLECULAR CELL BIOLOGY
(2018)