期刊
BIOCHEMICAL JOURNAL
卷 429, 期 -, 页码 261-271出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20100291
关键词
extracellular localization; Malus domestica; pollen tube growth; polyamine; protein cross-link; transglutaminase
资金
- Italian Ministero dell'Universita [PAIN 2007, Progetto Strategic d'Ateneo Unibo 2006 Crossallergenicity]
- ESF
- COST Action 844
- Progetto Giovani Ricercatori (University of Bologna, Bologna, Italy)
An extracellular form of the calcium-dependent protein-cross-linking enzyme TGase (transglutaminase) was demonstrated to be involved in the apical growth of Mains domestica pollen tube. Apple pollen TGase and its substrates were co-localized within aggregates on the pollen tube surface, as determined by indirect immunofluorescence staining and the in situ cross-linking of fluorescently labelled substrates. TGase-specific inhibitors and an anti-TGase monoclonal antibody blocked pollen tube growth, whereas incorporation of a recombinant fluorescent mammalian TGase substrate (histidine-tagged green fluorescent protein: His(6)-Xpr-GFP) into the growing tube wall enhanced tube length and germination, consistent with a role of TGase as a modulator of cell wall building and strengthening. The secreted pollen TGase catalysed the cross-linking of both PAs (polyamines) into proteins (released by the pollen tube) and His(6)-Xpr-GFP into endogenous or exogenously added substrates. A similar distribution of TGase activity was observed in planta on pollen tubes germinating inside the style, consistent with a possible additional role for TGase in the interaction between the pollen tube and the style during fertilization.
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