Article
Biochemistry & Molecular Biology
Xinru Wu, Junjie Zhu, Liming Zhu, Yao Tang, Zhaodong Hao, Jingbo Zhang, Jisen Shi, Tielong Cheng, Lu Lu
Summary: In this study, 66 candidate proteins were identified from the halophyte Nitraria sibirica, and their potential involvement in abiotic stress and hormone responses was predicted. Transcriptome analysis revealed upregulation of certain NsCMLs under salt treatment and similarity in gene expression patterns under salt and cold stress. Overexpression of specific NsCMLs mitigated H2O2 accumulation caused by salt stress.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Marine & Freshwater Biology
Changmei Li, Yilin Wang, Manman Wei, Xiudan Wang
Summary: This study reveals that marine calcifiers can adapt to ocean acidification through stimulating calcium binding/signaling pathways. A CaM-like gene (CgCaLP-2) from oyster Crassostrea gigas showed a stronger response to long-term CO2 exposure compared to short-term exposure. Two different splice variants of CgCaLP-2 were identified through genome sequence analysis. CgCaLP-2 may mediate the Ca2+-CaLP-TF signal transduction pathway under long-term CO2 exposure.
AQUATIC TOXICOLOGY
(2023)
Article
Virology
Shan Su, Xin Liu, Ren-Rong Tian, Kai-Xuan Qiao, Chang-Bo Zheng, Wen-Cong Gao, Liu-Meng Yang, Qiao-Zhen Kang, Yong-Tang Zheng
Summary: Zika virus interacts with the host cell membrane skeleton protein 4.1R during cell entry and viral protein synthesis, showing the important role of 4.1R in ZIKV infection.
Article
Chemistry, Physical
Rylee Wander, Andrea M. Kaminski, Zhangjie Wang, Eduardo Stancanelli, Yongmei Xu, Vijayakanth Pagadala, Jine Li, Juno M. Krahn, Truong Quang Pham, Jian Liu, Lars C. Pedersen
Summary: The study presents the crystal structures of 3-OST-5 with substrates and PAP, revealing its substrate specificity and anti-factor Xa activity. The enzyme prefers to sulfate a 6-O-sulfo glucosamine saccharide that is surrounded by glucuronic acid. Additionally, it was found that substrate specificity is not only determined by the side chains of amino acid residues in the active site, but also by the conformational flexibility of 2-O-sulfated iduronic acid in the saccharide substrates.
Article
Biochemistry & Molecular Biology
Akhmed Aslam, Jawwad Ahmad, Mohammed A. Baghdadi, Shakir Idris, Riyad Almaimani, Aiman Alsaegh, Mai Alhadrami, Bassem Refaat
Summary: This study found that vitamin D is superior to Paricalcitol in potentiating 5-FU cytotoxicity against colorectal cancer, possibly by upregulating several Ca2+-related molecules involved in tumor suppression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Oncology
Cong Ding, Yuying Guo, Taotao Liang, Jiaojiao Liu, Lu Yang, Ting Wang, Xin Liu, Qiaozhen Kang
Summary: Protein 4.1R, as a novel negative regulator, negatively regulates the proliferation of MCs by inhibiting C-Kit-mediated signal transduction, providing a potential target for the prevention and treatment of diseases related to abnormal MCs proliferation.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biology
Corentin Leger, Irene Pitard, Mirko Sadi, Nicolas Carvalho, Sebastien Brier, Ariel Mechaly, Dorothee Raoux-Barbot, Maryline Davi, Sylviane Hoos, Patrick Weber, Patrice Vachette, Dominique Durand, Ahmed Haouz, J. Inaki Guijarro, Daniel Ladant, Alexandre Chenal
Summary: We conducted a molecular characterization of CaM:CDZ complexes using SEC-SAXS, X-ray crystallography, HDX-MS, and NMR methods. The binding of CDZ causes conformational reorientation of CaM and stabilizes its structural elements, leading to reduced protein dynamics. Residues involved in the stabilization of the CaM:CDZ complex were identified. These findings provide insights into CDZ-induced dynamics and structural changes of CaM, which can be used for the design of more selective CaM antagonists.
Article
Pharmacology & Pharmacy
Claire Normand, Billy Breton, Marine Salze, Eric Barbeau, Arturo Mancini, Martin Audet
Summary: Prostaglandins are bioactive lipids involved in various physiological and pathophysiological processes. This study investigated the signaling profiles of different isoforms of the human EP3 receptor, which is activated by the bioactive lipid PGE2. The research revealed potential differences in signaling mechanisms between isoforms and identified a specific antagonist with biased agonist effects on the Gαz pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Oncology
Yu Lu, Dandan Fan, Wen Wang, Xian Gao, Hanhan Li, Shuangshuang Guo, Luyang Zhao, Yaxin Guo, Bowen Li, Yali Zhong, Binglei Zhang, Jingjing Liu, Liping Dai, Qiaozhen Kang, Zhenyu Ji
Summary: The knockout of protein 4.1R reduces phagocytosis in M2 macrophages, promotes the development of colon cancer, and drives tumor progression by activating the PI3K/AKT signaling pathway.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Parasitology
Hai-Jun Gao, Xu-Dong Sun, Yan-Ping Luo, Hua-Sheng Pang, Xing-Ming Ma, Ting Zhang, Tao Jing, Wei Hu, Yu-Juan Shen, Jian-Ping Cao
Summary: The study demonstrated that verapamil has anti-parasitic effects against Echinococcus by downregulating the expression of Ca2+/CaM-CaMKII, indicating that Ca2+/CaM-CaMKII may be a novel drug target for the treatment of echinococcosis.
PARASITES & VECTORS
(2021)
Article
Multidisciplinary Sciences
Ana Claudia Amaral, Beatriz G. Perez-Nievas, Michael Siao Tick Chong, Alicia Gonzalez-Martinez, Herminia Argente-Escrig, Sara Rubio-Guerra, Caitlin Commins, Serra Muftu, Bahareh Eftekharzadeh, Eloise Hudry, Zhanyun Fan, Prianca Ramanan, Shuko Takeda, Matthew P. Frosch, Susanne Wegmann, Teresa Gomez-Isla
Summary: Partial reduction of GSK-3 beta can decrease pathological tau changes in the brain of AD mice, including hyperphosphorylation and aggregation, reducing tau protein spread between neurons. This suggests GSK-3 beta as a promising therapeutic target for AD and other tauopathies.
Review
Cell Biology
Alessia Remigante, Rossana Morabito, Angela Marino
Summary: Band 3 protein (B3p) is an anion transporter that plays a crucial role in erythrocyte homeostasis and cell shape, particularly under oxidative stress conditions. Investigating the functions and anion exchange capability of B3p is significant for understanding related diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Weiyi She, Hao Wang, Darwin Linardi, Sin Yu Chik, Yi Lan, Feng Chen, Aifang Cheng, Pei-Yuan Qian
Summary: Marine biofouling causes economic losses, while Albofungin shows potential as an antifoulant. A study investigated the changes in the transcriptome and proteome of Amphibalanus amphitrite cyprids under albofungin treatment. The results revealed the mechanism of albofungin interrupting larval settlement and its potential as an antifouling agent.
Article
Biochemical Research Methods
Sichao Qiu, Guoxian Yu, Xudong Lu, Carlotta Domeniconi, Maozu Guo
Summary: Motivation: High-resolution annotation of gene functions is crucial in functional genomics. This study proposes IsofunGO, an efficacy approach, to differentiate the functions of isoforms using GO embedding. It introduces an attributed hierarchical network to model massive GO terms and develops an attention-based MIL network to predict isoform functions. Both GO embedding and attention mechanism boost performance and interpretability.
Article
Biochemistry & Molecular Biology
Rossana Morabito, Alessia Remigante, Marika Cordaro, Vincenzo Trichilo, Saverio Loddo, Silvia Dossena, Angela Marino
Summary: This study found that acute inflammation affects the function of erythrocytes, leading to increased anion exchange capability of the Band 3 protein, increased lipid peroxidation, and decreased -SH groups and GSH content. Serum CRP level recovery is associated with the recovery of -SH groups and GSH, but not with the restoration of anion exchange capability. After 2 months, all parameters were completely recovered.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)