Article
Multidisciplinary Sciences
Luke T. Izzo, Sophie Trefely, Christina Demetriadou, Jack M. Drummond, Takuya Mizukami, Nina Kuprasertkul, Aimee T. Farria, Phuong T. T. Nguyen, Nivitha Murali, Lauren Reich, Daniel S. Kantner, Joshua Shaffer, Hayley Affronti, Alessandro Carrer, Andrew Andrews, Brian C. Capell, Nathaniel W. Snyder, Kathryn E. Wellen
Summary: The metabolite acetyl-CoA is essential for lipid synthesis and histone acetylation. Citrate and acetate are the primary precursors for nuclear-cytoplasmic acetyl-CoA. This study shows that glucose and fatty acids can contribute to acetyl-CoA pools and histone acetylation, and acetylcarnitine can transport two-carbon units from mitochondria to the cytosol. Glucose can also support fatty acid synthesis in the absence of ACLY, through a carnitine responsive and carnitine acetyltransferase-dependent mechanism.
Article
Agriculture, Multidisciplinary
Xue-Ning Wang, Li-Li Hong, Jian-Qiang Kong
Summary: A stable acetyl-CoA-synthesizing biocatalyst was identified using maltose O-acetyltransferase (MAT) with diacerein as an acetyl donor, providing a promising and cost-effective alternative method for acetylation modifications. The mutant MAT-E125F showed the highest yield of acetyl-CoA production and could also synthesize glycosyl esters through acetylation or transesterification reactions. The multifunctional MAT displayed a potent capacity in synthesizing acetyl-CoA and glycosyl esters using diacerein as an acetyl donor.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Haoya Yao, Yaoqing Wang, Xiao Zhang, Ping Li, Lin Shang, Xiaocui Chen, Jia Zeng
Summary: Obesity and diabetes lead to mitochondrial dysfunction and hepatic lipid accumulation, while suppressing fatty acid synthesis and depleting malonyl-CoA in the liver. This study reveals that peroxisomal fatty acid oxidation may play a role in regulating mitochondrial fatty acid oxidation and inducing hepatic steatosis in obese or diabetic animals.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Agricultural Engineering
Yunxia Qin, Jia Wang, Yongjun Fang, Jilai Lu, Xiangyu Shi, Jianghua Yang, Xiaohu Xiao, Xuehua Luo, Xiangyu Long
Summary: Research has shown that anaerobic metabolism plays a crucial role in rubber biosynthesis in laticifers of Hevea brasiliensis. The key genes HbLDH1 and HbPDC4 in anaerobic metabolism are activated during tapping, resulting in the production of acetaldehyde and ATP through ethanol and lactate fermentation, which contribute to rubber synthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Medicinal
Shen You, Ming-Jin Wang, Zhen-Yan Hou, Wei-Da Wang, Ting-Ting Du, Ni-Na Xue, Ming Ji, Xiao-Guang Chen
Summary: This study aimed to assess the antitumor activity of chlorogenic acid (CHA) against neuroblastoma and reveal its mechanism of action in cell differentiation. The results showed that CHA induced the differentiation of neuroblastoma cells and exerted antitumor activity through the ACAT1-TPK1-PDH pathway. CHA is a potential drug candidate for neuroblastoma therapy.
Article
Biotechnology & Applied Microbiology
Eugenia Messina, Camilla Pires de Souza, Claudia Cappella, Simona Nicole Barile, Pasquale Scarcia, Isabella Pisano, Luigi Palmieri, Jean-Marc Nicaud, Gennaro Agrimi
Summary: The study identified the mitochondrial carrier YlCrc1 involved in the Carnitine/Acetyl-Carnitine shuttle in Y. lipolytica. Manipulation of YlCrc1 improved propionate utilization and its conversion into OCFAs. These genetic modifications and the use of a lipogenic medium led to enhanced accumulation of OCFAs in Y. lipolytica.
MICROBIAL CELL FACTORIES
(2023)
Article
Cell Biology
Hiroshi Matsuura, Akiko Kojima, Yutaka Fukushima, Yu Xie, Xinya Mi, Ravshan Z. Sabirov, Yasunobu Okada
Summary: The study found that endogenous ATP released through Maxi-Cl channels has a significant impact on the left ventricular contractile function of Langendorff-perfused mouse hearts, contributing to the transient positive inotropy observed during reperfusion after short-term hypoxia/ischemia in the heart.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Meng Wang, Zhe Zheng, Zheni Tian, Hao Zhang, Chenyu Zhu, Xiangyu Yao, Yixin Yang, Xia Cai
Summary: The function and expression regulation activities of Acetyl-CoA acetyltransferase (AACT) in Euphorbia kansui Liou (EkAACT) were investigated. The overexpression of EkAACT in Arabidopsis led to improvements in growth morphology and tolerance to abiotic stress, suggesting the crucial role of AACT in terpenoid biosynthesis and overall regulation.
Article
Chemistry, Multidisciplinary
Jun Chen, Tamara K. Singh, Sarah Al Nemri, Maheen Zaidi, Kelvin L. Billingsley, Jae Mo Park
Summary: Mitochondrial oxidative phosphorylation (OXPHOS) is sensitive to various biological factors and dysregulation of OXPHOS is observed in the development of many pathological conditions. Acetyl-l-carnitine (ALCAR) is an endogenous source of acetyl-coenzyme A (CoA) and can be used to assess OXPHOS. In this study, hyperpolarized (HP) [1-C-13]ALCAR was developed as a noninvasive probe for investigating cardiac tricarboxylic acid (TCA) cycle activity in vivo. This study demonstrates the first use of HP methods with [1-C-13]ALCAR to analyze mitochondrial function and TCA cycle activity in the heart.
Article
Biochemistry & Molecular Biology
Teruo Miyazaki, Yuho Nakamura-Shinya, Kei Ebina, Shoichi Komine, Song-Gyu Ra, Keisuke Ishikura, Hajime Ohmori, Akira Honda
Summary: The study demonstrates the important role of taurine and carnitine as buffers of mitochondrial acetyl-CoA in skeletal muscles during endurance exercises, suppressing the increase in the mitochondrial acetyl-CoA/free CoA ratio. Increased levels of acetylated forms of taurine and carnitine in the blood during endurance exercises indicate the buffering of the acetyl moiety in mitochondria.
Article
Biochemistry & Molecular Biology
Siraprapa Siritutsoontorn, Witchuda Sukjoi, Steven W. Polyak, Chareeporn Akekawatchai, Sarawut Jitrapakdee
Summary: HLCS plays a crucial role in breast cancer growth and migration by promoting the biotinylation of carboxylases. Depletion of biotin or reduced expression of HLCS inhibits cell proliferation and migration, leading to apoptosis in breast cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Congcong Lyu, Yukun Chen, Yanan Meng, Jichao Yang, Shu Ye, Zhipeng Niu, Issam EI-Debs, Nishith Gupta, Bang Shen
Summary: Toxoplasma gondii is capable of infecting humans and animals, and its metabolic flexibility plays a crucial role in its parasitic life cycle. This study identified the mitochondrial pyruvate carrier (MPC) complex in T. gondii, which is responsible for transporting cytosolic pyruvate into the mitochondrion. The deletion of MPC subunits resulted in impaired synthesis of acetyl-CoA and reduced carbon flux through glycolysis and the tricarboxylic acid (TCA) cycle. Although disruption of MPC proteins had only a modest effect on the parasite's virulence in mice, it highlights the metabolic plasticity of T. gondii.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Samia Falak, Muhammad Sulaiman Saeed, Naeem Rashid
Summary: This manuscript describes the recombinant expression, characterization, and structural analysis of Pyrobaculum calidifontis' Pcal_0029 pyruvate kinase. The recombinant Pcal_0029 was produced in a soluble and highly active form. The purified protein exhibited metal-dependent activity and high thermostability. In silico structural analysis revealed eight conserved regions, and mutational studies showed substitutions that resulted in decreased enzyme activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Siyuan Ma, Mengjiao Lv, Xiaohua Chen, Guoqing Zang, Zhenghao Tang, Yi Zhang, Weiwei Hu
Summary: Avasimibe enhances the immune response of HBV-specific CTL induced by lentivector. Mechanism experiments demonstrate that Avasimibe augments CTL activity by regulating cell membrane cholesterol levels. Animal experiments also confirm the enhancing effect of Avasimibe on HBV-specific CTL immune response.
ANTIVIRAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jason J. Hu, Jane K. J. Lee, Yun-Tao Liu, Clinton Yu, Lan Huang, Inna Aphasizheva, Ruslan Aphasizhev, Z. Hong Zhou
Summary: 3-methylcrotonyl-CoA carboxylase (MCC) is a biotin-dependent mitochondrial enzyme involved in leucine catabolism. We discovered that native MCC from Leishmania tarentolae (LtMCC) forms filaments and determined their structures. The filaments are composed of a6b6 LtMCCs stacked together, binding biotin non-covalently and lacking coenzyme A. The stacking immobilizes the biotin carboxylase domains, rendering the enzyme inactive. Our findings propose a new catalysis model for LtMCC and provide insights into polymerization in the carboxylase superfamily.
Article
Biochemistry & Molecular Biology
Vernon E. Anderson
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2015)
Article
Chemistry, Multidisciplinary
Shuming Zhang, Hong Gu, Haoyuan Chen, Emily Strong, Edward W. Ollie, Daniel Kellerman, Danni Liang, Masaru Miyagi, Vernon E. Anderson, Joseph A. Piccirilli, Darrin M. York, Michael E. Harris
CHEMICAL COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Guo-Fang Zhang, Sushabhan Sadhukhan, Rafael A. Ibarra, Stephanie M. Lauden, Chia-Ying Chuang, Sophia Sushailo, Priya Chatterjee, Vernon E. Anderson, Gregory P. Tochtrop, Henri Brunengraber
BIOCHEMICAL JOURNAL
(2012)
Article
Chemistry, Medicinal
Xiaochun Zhu, Xiaoxia Tang, Jianye Zhang, Gregory P. Tochtrop, Vernon E. Anderson, Lawrence M. Sayre
CHEMICAL RESEARCH IN TOXICOLOGY
(2010)
Article
Chemistry, Medicinal
Stephanie R. Harris, Guo-Fang Zhang, Sushabhan Sadhukhan, Hua Wang, Chuan Shi, Michelle A. Puchowicz, Vernon E. Anderson, Robert G. Salomon, Gregory P. Tochtrop, Henri Brunengraber
CHEMICAL RESEARCH IN TOXICOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Stephanie R. Harris, Guo-Fang Zhang, Sushabhan Sadhukhan, Anne M. Murphy, Kristyen A. Tomcik, Edwin J. Vazquez, Vernon E. Anderson, Gregory P. Tochtrop, Henri Brunengraber
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Fang Ye, Helene Lemieux, Charles L. Hoppel, Richard W. Hanson, Parvin Hakimi, Colleen M. Croniger, Michelle Puchowicz, Vernon E. Anderson, Hisashi Fujioka, Ed Stavnezer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Eric L. Christian, Vernon E. Anderson, Michael E. Harris
JOURNAL OF INORGANIC BIOCHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Junhwan Kim, Paul E. Minkler, Robert G. Salomon, Vernon E. Anderson, Charles L. Hoppel
JOURNAL OF LIPID RESEARCH
(2011)
Article
Oncology
Erik A. Bey, Kathryn E. Reinicke, Melissa C. Srougi, Marie Varnes, Vernon E. Anderson, John J. Pink, Long Shan Li, Malina Patel, Lifen Cao, Zachary Moore, Amy Rommel, Michael Boatman, Cheryl Lewis, David M. Euhus, William G. Bornmann, Donald J. Buchsbaum, Douglas R. Spitz, Jinming Gao, David A. Boothman
MOLECULAR CANCER THERAPEUTICS
(2013)
Article
Multidisciplinary Sciences
Ulf-Peter Guenther, Lindsay E. Yandek, Courtney N. Niland, Frank E. Campbell, David Anderson, Vernon E. Anderson, Michael E. Harris, Eckhard Jankowsky
Article
Multidisciplinary Sciences
Hong Gu, Shuming Zhang, Kin-Yiu Wong, Brian K. Radak, Thakshila Dissanayake, Daniel L. Kellerman, Qing Dai, Masaru Miyagi, Vernon E. Anderson, Darrin M. York, Joseph A. Piccirilli, Michael E. Harris
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Medicine, Research & Experimental
Chynna N. Broxton, Prabhjot Kaur, Manuela Lavorato, Smruthi Ganesh, Rui Xiao, Neal D. Mathew, Eiko Nakamaru-Ogiso, Vernon E. Anderson, Marni J. Falk
Summary: Dihydrolipoamide dehydrogenase (DLD) deficiency is a genetic disorder caused by mitochondrial depletion. By studying the nematode Caenorhabditis elegans, researchers found that DLD-1 deficiency leads to increased mitochondrial stress and impaired growth and neuromuscular function. The potential therapeutic effects of dichloroacetate and thiamine were demonstrated in this study.
Editorial Material
Biochemical Research Methods
Michael E. Harris, Vernon E. Anderson
MEASUREMENT AND ANALYSIS OF KINETIC ISOTOPE EFFECTS
(2017)