期刊
BIOCHEMICAL GENETICS
卷 47, 期 11-12, 页码 749-762出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10528-009-9274-y
关键词
Oogenesis; Spermatogenesis; Sperm decondensation; Carassius auratus gibelio; Carassius auratus
资金
- National Major Basic Research Program [2004CB117401]
- Chinese Academy of Sciences [KSCX2-YW-N020]
- Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]
- Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]
Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
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