Article
Oncology
Weijie Liao, Naihan Xu, Haowei Zhang, Weifang Liao, Yanzhi Wang, Songmao Wang, Shikuan Zhang, Yuyang Jiang, Weidong Xie, Yaou Zhang
Summary: Our study reveals that EPB41L4A-AS1/GCN5 complex inhibits glucose uptake by regulating histone and nonhistone acetylation or crotonylation, targeting GLUT4/2 and TXNIP. Inhibition of EPB41L4A-AS1 expression may be an effective strategy for the treatment of type 2 diabetes mellitus, as weaker glucose uptake ability is one of its major clinical features.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Jiemiao Hu, Xueqing Xia, Qingnan Zhao, Shulin Li
Summary: The acetylation of Rae-1 protein at lysines 80 and 87 by acetyltransferases GCN5 and PCAF protects it from shedding, activating NKG2D-dependent immune surveillance and potentially serving as a new target for NKG2D immunotherapy in cancer treatment.
Article
Chemistry, Multidisciplinary
Marina Toplak, Adelheid Nagel, Britta Frensch, Thorsten Lechtenberg, Robin Teufel
Summary: We report a novel regulatory principle for natural product biosynthesis involving an acetyltransferase. The acetyltransferase modifies a redox-tailoring enzyme, enabling pathway furcation and alternating pharmacophore assembly in rubromycin polyketide biosynthesis. This discovery provides new perspectives for improving enzyme activities and natural product titers.
Article
Biochemistry & Molecular Biology
Smitha George, Hannah R. Blum, Eliana F. Torres-Zelada, Grace N. Estep, Youssef A. Hegazy, Gina M. Speer, Vikki M. Weake
Summary: The Chiffon gene in Drosophila encodes two polypeptides, Chiffon-A and Chiffon-B, which form the DDK and CHAT complexes respectively. The interaction between Chiffon-B and Gcn5 is conserved in insect species such as the Australian sheep blowfly and yellow fever mosquito. Chiffon-B has features of acidic transcriptional activators and may play a crucial role in Dipterans.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemical Research Methods
Jingshu Li, Ye Cao, Yun Yang, Huanhuan Ma, Jing Zhao, Yaoyang Zhang, Nan Liu
Summary: Protein lysine acetylation is a dynamic PTM that regulates various cellular events, including aging. This study focuses on GCN5 and explores its acetylation substrates in vivo using Drosophila. The results reveal an increase in protein acetylation with age, potentially triggered by the progressive increase in endogenous fly Gcn5 expression. Overall, this study expands our understanding of GCN5 substrates and their contribution to the aging acetylome.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Chunpeng An, Lei Deng, Huawei Zhai, Yanrong You, Fangming Wu, Qingzhe Zhai, Alain Goossens, Chuanyou Li
Summary: The plant hormone jasmonate (JA) regulates plant immunity and adaptive growth through a genome-wide transcriptional program. This study uncovers the reversible acetylation of the corepressor TPL and its role in JA signaling. The histone acetyltransferase GCN5 mediates TPL acetylation, promoting its interaction with the adaptor protein NINJA and recruitment to MYC2 target promoters for transcriptional repression. Conversely, the histone deacetylase HDA6 weakens TPL-NINJA interaction and inhibits TPL recruitment, facilitating transcriptional activation. The balance between GCN5 and HDA6 maintains TPL acetylation homeostasis, determining the transcriptional state of JA-responsive genes.
Review
Genetics & Heredity
Onyekachi E. Ononye, Michael Downey
Summary: This review summarizes the types and functions of posttranslational modifications that regulate Gcn5 in both yeast and human cells. Common themes, unanswered questions, and strategies for future research are also outlined.
Article
Biochemistry & Molecular Biology
Wen Ge, Yajuan Gong, Ya Li, Ningxia Wu, Yuting Ruan, Tongpeng Xu, Yongqian Shu, Wen Qiu, Yingwei Wang, Chenhui Zhao
Summary: In this study, the researchers found that interleukin-17 (IL-17) is able to promote metastasis in non-small cell lung cancer (NSCLC). They also identified the potential mechanism behind IL-17-induced NSCLC cell metastasis. The results showed that IL-17, IL-17RA, GCN5, SOX4, and MMP9 were all increased in NSCLC tissues and IL-17-stimulated NSCLC cells, and IL-17 treatment enhanced cell migration and invasion. Further exploration revealed that IL-17-upregulated GCN5 and SOX4 could bind to the same region of the downstream MMP9 gene promoter, driving its transcription and promoting cell migration and invasion. By inhibiting the expression of GCN5, SOX4, or MMP9, the researchers observed reduced SOX4 acetylation, MMP9 induction, and metastatic nodule formation in NSCLC cells. Overall, this study suggests that the IL-17-GCN5-SOX4-MMP9 axis is closely associated with NSCLC metastasis.
MOLECULAR CARCINOGENESIS
(2023)
Article
Microbiology
Kristen M. Jew, Van Thi Bich Le, Kiana Amaral, Allysa Ta, Nina M. Nguyen May, Melissa Law, Nicole Adelstein, Misty L. Kuhn
Summary: Acetylation is a protein post-translational modification that can influence cellular processes. Two mechanisms of N epsilon-acetylation have been identified in bacteria - non-enzymatic/chemical acetylation and enzymatic acetylation. This study focused on determining the 3D location of lysine residues that are acetylated by E. coli enzymes and investigating their conservation in homologs. The results revealed discrepancies between linear amino acid sequences and 3D structures in assessing lysine residue conservation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Changhan Ouyang, Jian Li, Xiaoxu Zheng, Jing Mu, Gloria Torres, Qilong Wang, Ming-Hui Zou, Zhonglin Xie
Summary: This study demonstrates that Ulk1 deletion inhibits neointima formation by reducing autophagic degradation of KAT2A and increasing TUBA acetylation in VSMCs.
Article
Pharmacology & Pharmacy
Xiao-Ling Liang, Lan Ouyang, Nan-Nan Yu, Zheng-Hua Sun, Zi-Kang Gui, Yu-Long Niu, Qing-Yu He, Jing Zhang, Yang Wang
Summary: This study identifies a potential anticancer therapy strategy by inducing cell death through shifting the scission position of mitochondria. The pan-HDAC inhibitor pracinostat is found to activate the CDK5-Drp1 signaling pathway, leading to mitochondrial fission and showing potential in CRC treatment.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Yanchang Liu, Zhicheng Tong, Chen Wang, Runzhi Xia, Huiwu Li, Haoran Yu, Juehua Jing, Wendan Cheng
Summary: This study explored the impact of TiO2 nanotubes on bone cell differentiation, revealing that they can drive osteogenic differentiation by promoting the expression of F-actin and GCN5.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xiaoli Zheng, Jian Zhang, Yanfeng Bai, Jiaqi Wang, Mingfeng Jiang, Su Zeng, Lvhua Wang
Summary: This study revealed the significant role of OATP1A2 in tumour development and progression, with its high expression being associated with poor prognosis. Inhibition of OATP1A2 was shown to suppress the progression and prognosis of esophageal squamous cell carcinoma (ESCC).
Review
Biochemistry & Molecular Biology
Michael Downey
Summary: GCN5 and PCAF are lysine acetyltransferase enzymes that play important roles in histone acetylation and non-histone substrate acetylation in eukaryotes. This review focuses on the current understanding of non-histone acetylation by GCN5 and PCAF in budding yeast and mammalian systems, highlighting the diverse impact on cell physiology. Critical caveats and open questions are defined at the end of the review for all models.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Cell Biology
Meng Xu, Ling Lin, Babul Moni Ram, Omprakash Shriwas, Kun-Han Chuang, Siyuan Dai, Kuo-Hui Su, Zijian Tang, Chengkai Dai
Summary: It has been found that heat shock factor 1 (HSF1), the master transcriptional regulator of the heat shock response, serves as a key modifier of c-MYC-mediated transcription. HSF1 deficiency reduces c-MYC DNA binding and suppresses its transcriptional activity throughout the genome. Mechanistically, c-MYC, MAX, and HSF1 form a transcription factor complex on genomic DNA, and surprisingly, the DNA binding of HSF1 is not necessary. Instead, HSF1 physically recruits the histone acetyltransferase GCN5, promoting histone acetylation and enhancing c-MYC transcriptional activity.
Article
Biochemistry & Molecular Biology
Dasom Mun, Hyoeun Kim, Ji-Young Kang, Hyelim Park, Hyewon Park, Seung-Hyun Lee, Nuri Yun, Boyoung Joung
Article
Medicine, Research & Experimental
Ji-Young Kang, Hyewon Park, Hyoeun Kim, Dasom Mun, Hyelim Park, Nuri Yun, Boyoung Joung
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2019)
Article
Multidisciplinary Sciences
Hyoeun Kim, Ji-Young Kong, Dasom Mun, Nuri Yun, Boyoung Joung
Article
Clinical Neurology
Young Hee Kim, Young Hye Kim, Yoon Kyung Shin, Young Rae Jo, Da Kyeong Park, Min-Young Song, Byeol-A Yoon, Soo Hyun Nam, Jong Hyun Kim, Byung-Ok Choi, Ha Young Shin, Seung Woo Kim, Se Hoon Kim, Young Bin Hong, Jong Kuk Kim, Hwan Tae Park
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Kyung-Ah Park, Nuri Yun, Young J. Oh
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Cell Biology
Yeon Uk Ko, Chiho Kim, Juhyung Lee, Dana Kim, Yoonkyung Kim, Nuri Yun, Young J. Oh
CELL DEATH & DISEASE
(2019)
Article
Pharmacology & Pharmacy
Soo Youn Lee, Min-Young Song, Dain Kim, Chaewon Park, Da Kyeong Park, Dong Geun Kim, Jong Shin Yoo, Young Hye Kim
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Medicine, Research & Experimental
Ji-Young Kang, Hyoeun Kim, Dasom Mun, Nuri Yun, Boyoung Joung
Article
Biochemistry & Molecular Biology
Xiao-Xiao Zhao, Haneul Cho, Sora Lee, Jong Shin Woo, Min-Young Song, Xian Wu Cheng, Kyung Hye Lee, Weon Kim
CHEMICO-BIOLOGICAL INTERACTIONS
(2020)
Article
Neurosciences
Yeon Uk Ko, Hwa Young Song, Won-Ki Kim, Tae Young Yune, Nuri Yun, Young J. Oh
NEUROSCIENCE LETTERS
(2020)
Article
Biochemistry & Molecular Biology
Min-Young Song, Ji Yeon Hwang, Eun Ji Bae, Saesbyeol Kim, Hye-Min Kang, Yong Jun Kim, Chan Park, Kang-Sik Park
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Multidisciplinary
Ji-Young Kang, Hyoeun Kim, Dasom Mun, Nuri Yun, Boyoung Joung
Summary: By functionalizing extracellular vesicles (EVs) with a cardiac-targeting peptide (CTP) and loading curcumin and miR144-3p simultaneously, enhanced targeting ability, bioavailability, and cardioprotective effects of therapeutic molecules can be achieved, providing a promising nanomedicine strategy for MI therapy.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Cardiac & Cardiovascular Systems
Haneul Cho, Xiao-Xiao Zhao, Sora Lee, Jong Shin Woo, Min-Young Song, Xian Wu Cheng, Kyung Hye Lee, Weon Kim
Summary: The cardiotoxicity of DOX is mainly attributed to mitochondrial iron accumulation and ROS production, which are independent of its anti-tumor DNA damaging effects. The sGC-cGMP signaling pathway plays a crucial role in chemo-sensitization and myocardial cell survival. The cGMP signaling is also regulated by PDEs, which protect the heart from damage.
AMERICAN JOURNAL OF CARDIOVASCULAR DRUGS
(2022)
Article
Biology
Min-Young Song, Haneul Cho, Sora Lee, Kyung Hye Lee, Weon Kim
Summary: DSHT exerts beneficial anti-atherosclerotic effects by regulating cholesterol metabolism through the activation of the PPAR gamma signaling pathway and promoting anti-inflammatory M2 macrophage polarization.
Article
Cell Biology
Chang-Ki Oh, Young Ki Choi, Ih-Yeon Hwang, Yeon Uk Ko, In Kwon Chung, Nuri Yun, Young J. Oh
CELL DEATH & DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
