期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 449, 期 2, 页码 202-207出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.04.161
关键词
Flo8; LisH motif; Transcriptional activation; Mss11; FLO1; FLO11
资金
- DGIST RD
- MIRE-BraiN program
- Basic Science Research Program through the Ministry of Science, ICT & Future Planning of Korea [14-BD-0402, 2013R1A1A1004978]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2013R1A1A1008634, 2012R1A3A2026454]
- National Research Foundation of Korea - Korean Government [NRF-2013S1A2A2035342]
- Ministry of Science, ICT & Future Planning, Republic of Korea [14-BD-0402] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2013S1A2A2035342, 2013R1A1A1004978, 2013R1A1A1008634] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11 and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively. However, the molecular mechanism of Flo8-mediated transcriptional activation remains largely elusive. By generating serial deletion constructs, we revealed here that a novel transcriptional activation domain on its extreme C-terminal region plays a crucial role in activating transcription. On the other hand, the N-terminal LisH motif of Flo8 appears to be required for its physical interaction with another transcriptional activator, Mss11, for their cooperative transcriptional regulation of the shared targets. Additionally, GST pull-down experiments uncovered that Flo8 and Mss11 can directly form either a heterodimer or a homodimer capable of binding to DNA, and we also showed that this formed complex of two activators interacts functionally and physically with the Swi/Snf complex. Collectively, our findings provide valuable clues for understanding the molecular mechanism of Flo8-mediated transcriptional control of multiple targets. (C) 2014 Elsevier Inc. All rights reserved.
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