Article
Nanoscience & Nanotechnology
Ni Meng, Xiaolong Cheng, Mengyao Sun, Yushan Zhang, Xueke Sun, Xifu Liu, Jing Chen
Summary: This study identified specific nanobodies targeting the monkeypox virus protein A35R, which can serve as potential candidates for the development of monkeypox vaccines and diagnostic kits. The nanobodies displayed high affinity and specificity for A35R, laying the foundation for further research and applications in diagnosing and treating monkeypox.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Medicine, Research & Experimental
Mahmoud Ganji, Pooria Safarzadeh Kozani, Fatemeh Rahbarizadeh
Summary: Monoclonal antibody-based immunotherapies have shown promising results in treating immunological and oncological conditions. CD19 is a qualified antigen for B-cell neoplasms treatment, and VHHs have advantages over conventional mAbs as potential therapeutics and diagnostic tools. By isolating CD19-specific VHHs from a novel immune library using phage display, it was demonstrated that selected VHHs exhibited high specificity, sensitivity, and affinity to CD19, making them potential targeting tools for CD19-based immunotherapeutics.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Fisheries
Ai-Guo Huang, Wei-Hao He, Lin-Jun Su, Fa-Li Zhang, Ying-Hui Wang
Summary: In the study, GBS-specific nanobody Nb01 was obtained through screening and found to effectively inhibit GBS infection in zebrafish, reducing GBS copy numbers and increasing survival rate, highlighting the potential therapeutic activity of Nbs against GBS in fish.
Article
Immunology
Rifei Li, Xinjie Zhu, Peng Zhou, Yuehua Qiao, Yinqian Li, Yice Xu, Xi Shi
Summary: This study successfully identified a specific anti-CD147 nanobody and demonstrated its potential applications in tumor targeting and anti-tumor effects. Furthermore, it showed a synergistic effect when combined with doxorubicin.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Pakpoom Navanukraw, Sroisuda Chotimanukul, Taratorn Kemthong, Kiattawee Choowongkomon, Kaywalee Chatdarong
Summary: This study developed a novel form of contraception for long-tailed macaques by reducing sperm output without altering testosterone levels. It offers promising insights into the development of new methods of contraception for long-tailed macaques.
Article
Biochemistry & Molecular Biology
Yunier Serrano-Rivero, Julieta Salazar-Uribe, Marcela Rubio-Carrasquilla, Frank Camacho-Casanova, Oliberto Sanchez-Ramos, Alain Gonzalez-Pose, Ernesto Moreno
Summary: This study successfully generated anti-EGF nanobodies from a synthetic library, using a selection strategy involving four different elution steps and three rounds of selection. The results demonstrate the feasibility of selecting nanobodies against small antigens like EGF from synthetic libraries.
Article
Chemistry, Medicinal
Laura Jo Sherwood, Andrew Hayhurst
Summary: In this study, nanobodies with uniform cross-reactivity to different species of the Ebolavirus genus were isolated by immunizing a llama. These nanobodies exhibited equivalent reactivities across all five species and greatly enhanced sensitivity in immunodiagnostic assays and live virus titrations. They also showed recognition capability towards a newly discovered Ebola virus species. The simplicity and modularity of these nanobodies have potential applications in antigen-based diagnostic technologies.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Ahmed Mohammed, Weihong Zeng, Hylemariam Mihiretie Mengist, Arnaud John Kombe Kombe, Huichao Ou, Yunru Yang, Zhao Dan, Zhihao Xu, Huan Ma, Tengchuan Jin
Summary: The study developed and characterized V-C1 domain-specific Nbs targeting human RAGE, which exhibited high thermostability and improved binding affinity after homodimerization. Competitive ELISA demonstrated their potential in blocking V-C1-hS100B, offering new insights into treating RAGE-associated diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Microbiology
Yaru Zhao, Jifei Yang, Qingli Niu, Jinming Wang, Mengyao Jing, Guiquan Guan, Meng Liu, Jianxun Luo, Hong Yin, Zhijie Liu
Summary: African swine fever is a deadly disease in domestic and wild swine, causing significant economic losses. This study successfully constructed a high-quality phage display library containing specific nanobodies against ASFV proteins. Two nanobodies, Nb17 and Nb30, were used to develop a sandwich ELISA for the detection of ASFV in clinical specimens. The newly developed assay showed high sensitivity and specificity, providing a valuable alternative technique for ASF detection and surveillance.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kui Gu, Zengxu Song, Peng Ma, Ziwei Liao, Ming Yang, Changyu Zhou, Chao Li, Yu Zhao, Hao Li, Xin Yang, Changwei Lei, Hongning Wang
Summary: A rapid, simple, sensitive, and specific competitive ELISA (cELISA) was developed in this study for the detection of Avian coronavirus-infectious bronchitis virus (AvCoV-IBV) antibodies. The developed cELISA showed high consistency with commercial ELISA, fast operation, and high sensitivity, making it potential for disease monitoring and control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Paul Cordell, Glenn Carrington, Alistair Curd, Francine Parker, Darren Tomlinson, Michelle Peckham
Summary: Antibodies, nanobodies, and Affimers are tools used for labeling molecules in cells. Nanobodies and Affimers have advantages over traditional antibodies due to their small size, high binding affinity, and ability to penetrate dense cellular regions. They are expected to become the preferred tools for super-resolution imaging.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ailin Zhang, HuiJuan Zhao, Shaohan Pei, Yue Chi, Xiuhua Fan, Jianqing Liu
Summary: This study identified the key allergenic epitopes of cashew nut proteins, Ana o 2 and Ana o 3, and found that Ana o 3 may be more allergenic than Ana o 2.
Article
Virology
Yu-Ming Gong, Xue-Feng Wei, Yu-Ying Zheng, Yang Li, Qing Yu, Peng-Fei Li, Bin Zhu
Summary: This study identified an antigen epitope, Pep3, that provided protection against TiLV in fish. The study also identified the protective epitope S1(399-410) and demonstrated its effectiveness in neutralizing TiLV. This research provides a new approach for screening antigen epitopes of emerging viral diseases.
JOURNAL OF VIROLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qianlin Li, Fuqiang Zhang, Yi Lu, Huan Hu, Jin Wang, Cheng Guo, Qiang Deng, Conghui Liao, Qin Wu, Tingsong Hu, Zeliang Chen, Jiahai Lu
Summary: In this study, we developed potent multivalent VHH antibodies targeting the E2 glycoprotein of CHIKV, with Nb-2E8 and Nb-3C5 showing high-affinity binding within the nanomolar range.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Armina Alagheband Bahrami, Mojgan Bandehpour, Bahman Khalesi, Bahram Kazemi
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2020)
Article
Chemistry, Physical
Ammar Taghavi, Fatemeh Rahbarizadeh, Sara Abbasian, Ahmad Moshaii
Article
Biochemistry & Molecular Biology
Amin Barkhordari, Abbas Behzad-Behbahani, Vahid Jajarmi, Mojgan Bandehpour, Gholamreza Rafiei-Dehbidi, Fatemeh Safari, Fereydoun Mahboudi, Bahram Kazemi
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Akram Jalali, Mojgan Bandehpour, Rouzbeh Chegeni, Hossein Ghanbarian, Yasaman Mardani-Dashti, Sayyed Hamid Zarkesh-Esfahani, Bahram Kazemi
Summary: Using Leishmania tarentolae as an expression system for producing therapeutic recombinant proteins is cost-effective, efficient, and requires less labor.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Review
Oncology
Mohadese Hashem Boroojerdi, Fatemeh Rahbarizadeh, Pouya Safarzadeh Kozani, Elahe Kamali, Pooria Safarzadeh Kozani
Article
Biotechnology & Applied Microbiology
Reyhaneh Hoseinpoor, Bahram Kazemi, Masoumeh Rajabibazl, Azam Rahimpour
JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Elahe Kamali, Fatemeh Rahbarizadeh, Zohreh Hojati, Morten Frodin
Summary: CRISPR/Cas9 technology holds great potential in cancer immunotherapy, but genetic knockout in human T cells remains challenging. Knockout through electroporation of CRISPR/Cas9 plasmids is technically feasible, albeit with low efficiency.
Article
Clinical Neurology
Fardin Soleimanipour, Ehsan Razmara, Fatemeh Rahbarizadeh, Elnaz Fallahi, Mehrnoosh Khodaeian, Ali Reza Tavasoli, Masoud Garshasbi
Summary: Progressive myoclonus epilepsies (PMEs) are a group of disorders characterized by myoclonus, seizures, and neurological dysfunctions. This study identified a novel homozygous missense variant in the LMNB2 gene as a likely disease-causing variant for a consanguineous Iranian family with autosomal recessive PME. It highlights the challenges in diagnosing PMEs due to genetic heterogeneity and underscores the potential of next-generation sequencing in genetic diagnosis of patients with neurologic diseases.
ACTA NEUROLOGICA BELGICA
(2022)
Review
Oncology
Pooria Safarzadeh Kozani, Pouya Safarzadeh Kozani, Fatemeh Rahbarizadeh, Shahryar Khoshtinat Nikkhoi
Summary: CAR T cell therapy has shown promise in treating hematologic malignancies, but faces challenges such as toxicities and tumor microenvironment suppressive effects. Addressing these issues is crucial to bridge the gap between successful clinical outcomes and the hope for them.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Pooria Safarzadeh Kozani, Pouya Safarzadeh Kozani, Fatemeh Rahbarizadeh
Summary: CAR-T therapy has shown reliable therapeutic effects in treating certain hematologic malignancies, but faces challenges in treating solid tumors. Recent advancements in bioinformatics and cell biology offer hope for discovering novel tumor antigens, with some new CAR-T therapies showing promising clinical achievements.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nasrin Mohammadi, Mojgan Bandehpour, Fattah Sotoodehnejadnematalahi, Bahram Kazemi
Summary: The study successfully designed and expressed the Ecarin metalloproteinase, and verified its enzyme activity through experiments. Combining the predictive results from the I-TASSER server, it helped to better understand the relationship between the structure and function of the Ecarin metalloproteinase.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Pharmacology & Pharmacy
Aref Shiralipour, Babak Khorsand, Leila Jafari, Mohammad Salehi, Mahsa Kazemi, Javad Zahiri, Vahid Jajarmi, Bahram Kazemi
Summary: This study analyzed the problem of drug resistance in breast cancer using computational and systems biology approaches. Six key lysosome-related genes were identified as potential therapeutic targets for drug-resistant breast cancer.
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2022)
Article
Plant Sciences
Elnaz Bafandeh, Farnaz Kheirandish, Zeinab Hosseini, Saeed Veiskarami, Marzieh Rashidipour, Bahram Kazemi, Atiyeh Darbandi, Shirzad Fallahi, Arian Karimi Rouzbahani
Summary: Olive leaf extract (OLE) can significantly reduce the expression of DHFR gene and have a slight effect on the expression of PTR1 gene in Leishmania major parasite.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Cell Biology
Alireza Rajabzadeh, Fatemeh Rahbarizadeh, Davoud Ahmadvand, Maryam Kabir Salmani, Amir Ali Hamidieh
Summary: The study developed an anti-MUC1 CAR based on camelid heavy chain antibodies, showing enhanced effectiveness in T cell immunotherapy for cancer treatment. The novel VHH-based CAR demonstrated high CAR surface expression, increased Th1 cytokine secretion, and enhanced cytotoxic activity against MUC1-positive tumor cells, indicating potential clinical applications in cancer immunotherapy.
Article
Cell Biology
Ommolbanin Asadpour, Fatemeh Rahbarizadeh
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)