Editorial Material
Biochemistry & Molecular Biology
Leonie Unterholzner
Summary: The DNA sensor cGAS and its adaptor STING form an ancient pathogen detection mechanism, but its conservation across the animal kingdom is uncertain. In this article in Cell, Kranzusch and colleagues identify numerous cGAS-like receptors and uncover networks of second messengers that activate innate immune responses in animals.
Article
Biotechnology & Applied Microbiology
Yu-Ming Cai, Kai-Wei Yu, Ji-Hong Liu, Zhao Cai, Zun-Hao Zhou, Yang Liu, Tian-Fu Wang, Liang Yang
Summary: The c-di-GMP signaling pathways in P. aeruginosa are highly organized and play important roles in the transition between motile and sessile lifestyles. This study identified a phosphodiesterase, PipA, that regulates bacteriophage production, and uncovered a potential signaling circuit for biofilm treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Shubhanjali Minhas, Alex S. Holehouse
Summary: The study reveals a conserved viral mechanism in which herpesvirus tegument proteins suppress cGAS:DNA phase separation, thereby subverting the innate immune system.
Article
Multidisciplinary Sciences
Calvin K. Lee, William C. Schmidt, Shanice S. Webster, Jonathan W. Chen, George A. O'Toole, Gerard C. L. Wong
Summary: This study reveals that changes in c-di-GMP levels in bacterial biofilms are not the sole factor determining surface commitment, as oscillations in c-di-GMP levels can propagate across generations. Additionally, the study demonstrates that modulation of c-di-GMP signals and cooperative surface commitment can be influenced by varying the interaction strength between PilO and SadC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Dingbin Li, Li Zhang, Jinhua Liang, Wusheng Deng, Qingjun Wei, Ke Wang
Summary: This study established a rabbit model of septic arthritis caused by Pseudomonas aeruginosa, demonstrating the formation of biofilms in the knee joint cavity. Scanning electron microscopy and PNA-FISH assays were used to observe the biofilm structures and fiber deposition in the synovial membrane. The findings suggest that c-di-GMP plays a key role in biofilm formation in the rabbit models.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Emily A. Williams McMackin, Jodi M. Corley, Sardar Karash, Jeremiah Marden, Matthew C. Wolfgang, Timothy L. Yahr
Summary: The transcription factor Vfr plays a crucial role in regulating the activity of the P-Rha and P-BAD promoter systems in Pseudomonas aeruginosa, potentially leading to significant variability in promoter activity in different genetic backgrounds.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Hend Selim, Tharwat E. E. Radwan, Amany M. Reyad
Summary: T3SS, an important virulence factor of Pseudomonas aeruginosa, plays a central role in the infection process. However, the functional regulation of T3SS by environmental signals remains poorly understood. Studies have shown that T3SS appears as bright foci at the cell membrane of P. aeruginosa with tight temporal and spatial control. Interestingly, T3SS assembly and ExoS synthesis increase under high salt concentration but are unaffected by different cyclic di-GMP levels. Furthermore, T3SS itself affects the cyclic di-GMP levels inside the cell.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Cell Biology
Youzhou Tang, Xinyu Li, Yafang Wei, Yongchao Sun, Yeyi Yang, Xianming Zhang, Zhihao Gao, Jishi Liu, Quan Zhuang
Summary: This study identified significant differential expression of eight acetyltransferase and deacetylase family members in SLE, with KAT2A being upregulated and positively correlated with disease activity index. KAT2A was found to modulate cGAS pathway through increasing expression and post-translational modification, specifically in certain immune cell subsets in SLE.
CELL DEATH & DISEASE
(2021)
Article
Ecology
Shaoyang Li, Sylvia Yang Liu, Shepherd Yuen Chan, Song Lin Chua
Summary: Microbes use biofilm cloak as an anti-detection adaptation to hide their chemical trails from predators, which has a fundamental impact on the ecological dynamics of microbial communities and predator-prey interactions.
Article
Multidisciplinary Sciences
Siyu Guo, Zhao Liu, Yongshuai Yang, Jun Chen, Chun Loong Ho
Summary: This paper presents a simple gradient swarm plate method that provides qualitative and quantitative information about the inducers' effects on surface swarming.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Chemistry, Physical
Jordi Soler, Pedro Paiva, Maria J. Ramos, Pedro A. Fernandes, Marie Brut
Summary: This study utilizes Molecular Dynamics to investigate the activation of cGAS, focusing on the motion of the activation loop and its role in cGAMP production. Insights into the reaction resulting in cGAMP synthesis are provided, with two possible catalytic routes proposed based on active site occupancy. This paves the way towards further exploration of the reaction mechanism.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Xiangke Duan, Yanrong Pan, Zhao Cai, Yumei Liu, Yingdan Zhang, Moxiao Liu, Yang Liu, Ke Wang, Lianhui Zhang, Liang Yang
Summary: This study demonstrated that antibiotic treatment of biofilm-related P. aeruginosa infections could induce a hyperbiofilm phenotype through rpoS mutation, possibly contributing to the failure of antimicrobial treatment in these infections. The evolved hyperbiofilm mutants outcompeted the ancestral strain within biofilms but not in planktonic cultures, and the mutation in rpoS gene increased intracellular c-di-GMP level and virulence in the hyperbiofilm variants.
CELL AND BIOSCIENCE
(2021)
Article
Chemistry, Medicinal
Zheng-Yu Jiang, Qi-Dong You, Xiao-Li Xu, Nan-Nan Chen, Han Zhang, Qiang-Sheng Zhu, Ting Zeng, Wei Dai, Ye-Ling Zhou, Guo-Feng Xin, Bei-Duo Wu, Si-Jia Gong
Summary: By modifying and optimizing the structure of mono-aminobenzimidazole (ABZI), nanomolar STING agonists were obtained. Compounds D59 and D61 significantly increased the transcription of IFN-beta and CXCL10, and induced phosphorylation of STING downstream proteins in THP1 cells. Furthermore, compound D61 exhibited favorable pharmacokinetic properties and effectively inhibited tumor growth in a mouse tumor model.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Ecology
Shepherd Yuen Chan, Sylvia Yang Liu, Zijing Seng, Song Lin Chua
Summary: A study found that biofilms produced by the Gram-negative bacterium Pseudomonas aeruginosa can interfere with the motility of bacterivorous nematode Caenorhabditis elegans through the production of a specific exopolysaccharide, Psl. This interference reduces the ability of C. elegans to roam and prey on the biofilms.
Article
Oncology
Nada Chaoul, Alexandre Tang, Belinda Desrues, Marine Oberkampf, Catherine Fayolle, Daniel Ladant, Alexander Sainz-Perez, Claude Leclerc
Article
Pharmacology & Pharmacy
Darragh P. O'Brien, Ana Cristina Sotomayor Perez, Johanna Karst, Sara E. Cannella, Veronique Yvette Ntsogo Enguene, Audrey Hessel, Dorothee Raoux-Barbot, Alexis Voegele, Orso Subrini, Marilyne Davi, J. Inaki Guijarro, Bertrand Raynal, Bruno Baron, Patrick England, Belen Hernandez, Mahmoud Ghomi, Veronique Hourdel, Christian Malosse, Julia Chamot-Rooke, Patrice Vachette, Dominique Durand, Sebastien Brier, Daniel Ladant, Alexandre Chenal
Article
Pharmacology & Pharmacy
Alexander Belyy, Undine Mechold, Louis Renault, Daniel Ladant
Article
Food Science & Technology
Alexis Voegele, Mirko Sadi, Dorothee Raoux-Barbot, Thibaut Douche, Mariette Matondo, Daniel Ladant, Alexandre Chenal
Editorial Material
Immunology
Daniel Ladant, Carmen Buchrieser, Michel R. Popoff
PATHOGENS AND DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Darragh P. O'Brien, Sara E. Cannella, Alexis Voegele, Dorothee Raoux-Barbot, Marilyne Davi, Thibaut Douche, Mariette Matondo, Sebastien Brier, Daniel Ladant, Alexandre Chenal
Article
Multidisciplinary Sciences
Christelle Angely, Daniel Ladant, Emmanuelle Planus, Bruno Louis, Marcel Filoche, Alexandre Chenal, Daniel Isabey
Review
Food Science & Technology
Daniel Ladant
Summary: The adenylate cyclase toxin CyaA is a key virulent factor produced by Bordetella pertussis, targeting innate immunity to facilitate bacterial colonization of the respiratory tract. With remarkable characteristics, CyaA has been utilized for various applications in vaccinology and biotechnology. Its engineering as a vaccine vehicle for antigen delivery and as a genetic assay for monitoring protein-protein interactions in bacteria are briefly summarized in this chapter.
Article
Chemistry, Multidisciplinary
Alexis Voegele, Mirko Sadi, Darragh Patrick O'Brien, Pauline Gehan, Dorothee Raoux-Barbot, Maryline Davi, Sylviane Hoos, Sebastien Brule, Bertrand Raynal, Patrick Weber, Ariel Mechaly, Ahmed Haouz, Nicolas Rodriguez, Patrice Vachette, Dominique Durand, Sebastien Brier, Daniel Ladant, Alexandre Chenal
Summary: The P454 segment of the CyaA toxin is capable of translocating across membranes and interacting with calmodulin, with key residues involved in membrane destabilization and calmodulin binding. Inhibitors of calmodulin can efficiently block CyaA internalization. This suggests a potential mechanism for the vectorial translocation of the toxin into host cells.
Editorial Material
Food Science & Technology
Daniel Ladant, Pascale Marchot, Sylvie Diochot, Gilles Prevost, Michel R. Popoff, Evelyne Benoit
Summary: This report presents the 27th annual meeting of the French Society of Toxinology, which focused on the dual nature of toxins and showcased the latest research findings on plant, fungal, algal, animal, and bacterial toxins.
Article
Microbiology
Hazel Silistre, Dorothee Raoux-Barbot, Federica Mancinelli, Flora Sangouard, Alice Dupin, Alexander Belyy, Vincent Deruelle, Louis Renault, Daniel Ladant, Lhousseine Touqui, Undine Mechold
Summary: ExoY is an effector injected by Pseudomonas aeruginosa into host cells, with prevalence among strains and the potential to partly alleviate the cytotoxic effects of other virulence factors under certain conditions.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Corentin Leger, Irene Pitard, Mirko Sadi, Nicolas Carvalho, Sebastien Brier, Ariel Mechaly, Dorothee Raoux-Barbot, Maryline Davi, Sylviane Hoos, Patrick Weber, Patrice Vachette, Dominique Durand, Ahmed Haouz, J. Inaki Guijarro, Daniel Ladant, Alexandre Chenal
Summary: We conducted a molecular characterization of CaM:CDZ complexes using SEC-SAXS, X-ray crystallography, HDX-MS, and NMR methods. The binding of CDZ causes conformational reorientation of CaM and stabilizes its structural elements, leading to reduced protein dynamics. Residues involved in the stabilization of the CaM:CDZ complex were identified. These findings provide insights into CDZ-induced dynamics and structural changes of CaM, which can be used for the design of more selective CaM antagonists.
Article
Food Science & Technology
Marilyne Davi, Mirko Sadi, Irene Pitard, Alexandre Chenal, Daniel Ladant
Summary: Various bacterial pathogens are producing toxins that target the cyclic Nucleotide Monophosphate (cNMPs) signaling pathways in order to facilitate host colonization. Here we describe a simple and robust in vitro assay for AC activity based on the spectrophotometric detection of cyclic AMP (cAMP) after chromatographic separation on aluminum oxide.
Editorial Material
Food Science & Technology
Daniel Ladant, Gilles Prevost, Michel R. Popoff, Evelyne Benoit
Review
Biochemistry & Molecular Biology
Darragh P. O'Brien, Sebastien Brier, Daniel Ladant, Dominique Durand, Alexandre Chenal, Patrice Vachette
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)