Article
Biochemistry & Molecular Biology
Vineet Anand, Harshini Sheeja Prabhakaran, Prerana Gogoi, Shankar Prasad Kanaujia, Manish Kumar
Summary: By studying the Cas2C protein of two Leptospira serovars, it was found that in the absence of a CRISPR array, Cas2C may possess catalytic activity in biological processes distinct from the CRISPR-Cas-associated function.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Rodrigo Achigar, Martina Scarrone, Genevieve M. Rousseau, Cecile Philippe, Felipe Machado, Valentina Duvos, Maria Pia Campot, Moira B. Dion, Yuyu Shao, Maria Julia Pianzzola, Sylvain Moineau
Summary: The study demonstrates that both CRISPR1 and CRISPR3 systems in Streptococcus thermophilus exhibit ectopic spacer acquisition, providing phage resistance. This phenomenon also appears to occur naturally in some strains of Streptococcus pyogenes, suggesting it is a general feature in type II-A systems.
Article
Microbiology
Chuan Wang, Yuze Yang, Shaoqing Tang, Yuanzi Liu, Yaqin Wei, Xuerui Wan, Yajuan Liu, Zhao Zhang, Yongjie Sunkang
Summary: The study investigates the relationship between the CRISPR-Cas system and the optimum growth temperature in thermophilic bacteria. It reveals that the number of CRISPR loci and the structural features of the repeat sequences are correlated with temperature.
Article
Microbiology
Da-Young Kang, Andy Kim, Jeong Nam Kim
Summary: This study reveals that the transcription of the CRISPR-Cas system in the oral pathogen S. mutans is directly regulated by the binding of CcpA and CodY, two global regulators. This regulation plays a crucial role in carbohydrate metabolism and (p)ppGpp synthesis, contributing to the maintenance of cellular metabolism balance and rapid response to environmental cues.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biotechnology & Applied Microbiology
Xiang Meng, Tian-gang Wu, Qiu-yue Lou, Kai-yuan Niu, Lei Jiang, Qing-zhong Xiao, Tao Xu, Lei Zhang
Summary: Cancer is caused by genetic alterations and is a leading cause of death worldwide. The study of disease development and treatment strategies at the genetic level is crucial in cancer research. The CRISPR-Cas system provides a potential approach for gene editing but requires efficient delivery methods for clinical application.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Microbiology
Richard Bauer, Dorina Haider, Aline Grempels, Rebecca Roscher, Stefanie Mauerer, Barbara Spellerberg
Summary: This study analyzed the CRISPR-Cas systems in Streptococcus anginosus and found two types of CRISPR-Cas II systems. One type contained a long csn2 gene, similar to Streptococcus thermophilus, while the other type contained a short csn2 gene, similar to S. pyogenes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Chao Zhang, Penghui Zhang, Hui Ren, Pengpeng Jia, Jingcheng Ji, Lei Cao, Peiwei Yang, Yuxin Li, Jie Liu, Zedong Li, Minli You, Xiaoman Duan, Jie Hu, Feng Xu
Summary: This article introduces a synthetic biology-based technology that can amplify RNA signals efficiently and accurately. The technology uses the SHARK strategy, which combines cascade amplification and tailored enzyme output, and demonstrates broad compatibility in different scenarios. SHARK shows significant potential in SARS-CoV-2 biosensors and cancer diagnosis.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Xin Li, Nan Bao, Zhen Yan, Xian-Zheng Yuan, Shu-Guang Wang, Peng-Fei Xia
Summary: Antibiotic resistance has caused severe health problems and millions of deaths. Environmental processes, particularly in the wastewater treatment sector, are crucial for preventing the spread of antibiotic resistance. A synthetic biology system called VADER was developed, which uses CRISPR-Cas immunity to degrade antibiotic resistance genes (ARGs) in wastewater treatment processes. VADER was successfully tested in degrading plasmid-borne ARGs and eliminating ARGs in Pseudomonas aeruginosa, demonstrating its potential for tackling antibiotic resistance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Microbiology
Song Hee Jeong, Ho Joung Lee, Sang Jun Lee
Summary: Engineering biology has advanced with the help of synthetic biology, which has standardized and platformized biological systems based on hierarchy, orthogonality, and modularity. Genome engineering is critical in manufacturing and designing synthetic cells with desired functions using bioparts from sequence databases. One of the tools, CRISPR-Cas system, composed of guide RNA and Cas nuclease, has the convenience of freely editing the genome. Various strategies have been developed to accurately edit the genome at the single nucleotide level.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Virology
Dior Beerens, Sandra Franch-Arroyo, Timothy J. Sullivan, Christian Goosmann, Volker Brinkmann, Emmanuelle Charpentier
Summary: Bacteriophages impose strong evolutionary pressure on their microbial hosts, leading bacteria to develop sophisticated immune systems for protection. Streptococcus pyogenes prophages play a significant role in the pathogen's virulence, and the bacteria utilize mechanisms such as the CRISPR-Cas system and membrane vesicles to defend against phage predation.
Review
Biotechnology & Applied Microbiology
Wenqian Li, Can Huang, Jingyu Chen
Summary: CRISPR/Cas system is a widely used genome editing tool, but its off-target effects, cytotoxicity, and low efficiency hinder its application in new species. Therefore, system optimization is necessary to improve targeted specificity, reduce toxicity, and enhance editing efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Yujiro Hirose, Masaya Yamaguchi, Tomoko Sumitomo, Masanobu Nakata, Tomoki Hanada, Daisuke Okuzaki, Daisuke Motooka, Yasushi Mori, Hiroshi Kawasaki, Alison Coady, Satoshi Uchiyama, Masanobu Hiraoka, Raymond H. Zurich, Masayuki Amagai, Victor Nizet, Shigetada Kawabata
Summary: The ADI pathway in Streptococcus pyogenes enhances bacterial survival and pathogenicity under low-glucose conditions by altering gene expression, particularly by upregulating virulence genes. By increasing the expression of virulence genes, including those encoding exotoxins, S. pyogenes augments its pathogenicity through the ADI pathway. Furthermore, arginine from filaggrin in the stratum corneum serves as a key substrate for the ADI pathway, promoting the pathogenicity of S. pyogenes on the skin.
Review
Biochemistry & Molecular Biology
Peipei Yin, Yi Zhang, Lingguang Yang, Yue Feng
Summary: Bacteria and archaea have developed the CRISPR-Cas system as an adaptive defense mechanism against mobile genetic elements, but these elements have also evolved anti-CRISPR proteins to counteract the immune defenses. This review focuses on the non-canonical inhibition strategies adopted by these proteins and provides insights into the ongoing arms race between prokaryotic hosts and their predators.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Ulri N. Lee, Xiaojing Su, Damielle L. Hieber, Wan-Chen Tu, Anika M. McManamen, Meg G. Takezawa, Grant W. Hassan, Tung Ching Chan, Karen N. Adams, Ellen R. Wald, Gregory P. DeMuri, Erwin Berthier, Ashleigh B. Theberge, Sanitta Thongpang
Summary: In this study, a novel lollipop-inspired sampling platform (CandyCollect) was developed to capture bacteria in saliva, improving the testing experience for strep throat. The device can be used in clinics or at home, integrating with telemedicine.
Review
Biotechnology & Applied Microbiology
Ali Kerem Kalkan, Fahreddin Palaz, Semeniuk Sofija, Nada Elmousa, Yuri Ledezma, Elise Cachat, Leonardo Rios-Solis
Summary: This review summarizes recent studies on the use of CRISPR-Cas systems to improve recombinant protein production in CHO cells. The advantages, limitations, and potential solutions of these systems are discussed, with focus on their impact on glycan composition. Furthermore, the promising future applications of CRISPR in CHO cells are highlighted.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)