期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 446, 期 4, 页码 857-862出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.03.018
关键词
Cell-penetrating peptides; Syndecan-4; PKC alpha; Macropinocytosis
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Takeda Science Foundation
- Collaborative Research Program of Institute for Chemical Research, Kyoto University
- Grants-in-Aid for Scientific Research [23790129, 25461673] Funding Source: KAKEN
Arginine-rich cell-penetrating peptides (CPPs) are promising carriers for the intracellular delivery of various bioactive molecules. However, many ambiguities remain about the molecular interplays on cell surfaces that ultimately lead to endocytic uptake of CPPs. By treatment of cells with octaarginine (R8), enhanced clustering of syndecan-4 on plasma membranes and binding of protein kinase C alpha (PKC alpha) to the cytoplasmic domain of syndecan-4 were observed; these events potentially lead to the macropinocytic uptake of R8. The cytoplasmic V domain of syndecan-4 made a significant contribution to the cellular uptake of R8, whereas the cytoplasmic Cl and C2 domains were not involved in the process. (C) 2014 Elsevier Inc. All rights reserved.
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