Article
Cell Biology
Praveen K. Patnaik, Carine Beaupere, Hanna Barlit, Antonia Maria Romero, Mitsuhiro Tsuchiya, Michael Muir, Maria Teresa Martinez-Pastor, Sergi Puig, Matt Kaeberlein, Vyacheslav M. Labunskyy
Summary: This study reveals that defects in the mRNA-binding protein Cth2 contribute to iron dys-homeostasis, aging, and lifespan regulation in yeast. Modulation of Cth2 activity can impact mitochondrial function and extend lifespan.
Article
Geriatrics & Gerontology
Mitchell B. Lee, Michael G. Kiflezghi, Mitsuhiro Tsuchiya, Brian Wasko, Daniel T. Carr, Priya A. Uppal, Katherine A. Grayden, Yordanos C. Elala, Tu Anh Nguyen, Jesse Wang, Priya Ragosti, Sunny Nguyen, Yan Ting Zhao, Deborah Kim, Socheata Thon, Irika Sinha, Thao T. Tang, Ngoc H. B. Tran, Thu H. B. Tran, Margarete D. Moore, Mary Ann K. Li, Karl Rodriguez, Daniel E. L. Promislow, Matt Kaeberlein
Summary: Studies have shown that compounds like green tea extract and berberine have a shortening effect on yeast lifespan, while extracts from Pterocarpus marsupium and another mixture have a more significant lifespan-extending effect.
Article
Microbiology
Tianyu Zhao, Asaka Chida, Yuichi Shichino, Dongwoo Choi, Masaki Mizunuma, Shintaro Iwasaki, Yoshikazu Ohya
Summary: This study reveals the global translational regulation during replicative aging in yeast using ribosome profiling technique. The research found that the translational efficiency of genes involved in DNA repair and chromosome organization increased, while the translational efficiency of genes encoding ribosome components, transposons and cell wall assembly decreased during replicative aging.
Article
Multidisciplinary Sciences
Manuel Hotz, Nathaniel H. Thayer, David G. Hendrickson, Elizabeth L. Schinski, Jun Xu, Daniel E. Gottschling
Summary: This study discovered a previously unappreciated relationship between the number of copies of the ribosomal RNA gene and the replicative lifespan in budding yeast. The data suggest that regulatory mechanisms involving ribosomal DNA copy number limit lifespan and changes in rDNA copy number might explain the effects of lifespan-enhancing mutations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Barbara Schnitzer, Linnea Osterberg, Iro Skopa, Marija Cvijovic
Summary: Protein damage accumulation is a major driver of replicative ageing, and is closely linked to reactive oxygen and nitrogen species. Cells can adapt their metabolism to slow down damage accumulation, but older or damaged cells have less flexibility in allocating enzymes across the metabolic network, leading to further metabolic adaptations.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Cell Biology
Arman Ali Shah, Binghua Liu, Zhihuai Tang, Wang Wang, Wenjie Yang, Quanjun Hu, Yan Liu, Nianhui Zhang, Ke Liu
Summary: This study demonstrates that short-term treatment with fast H2S releasing donor NaHS at 96 hours after inoculation extends yeast lifespan, while treatments before 72 hours fail to do so. Early and late H2S treatments have similar effects on pathways related to lifespan regulation, but early treatment leads to unstable expression of antioxidant genes. Differences in gene regulation are also observed between early and late H2S treatment.
Article
Biochemistry & Molecular Biology
So-Young Yoon, Eunhong Jang, Naho Ko, Minseok Kim, Su Yoon Kim, Yeojin Moon, Jeong-Seok Nam, Sunjae Lee, Youngsoo Jun
Summary: The asymmetry in cytosolic pH between mother and daughter cells is believed to be responsible for cellular aging in budding yeast. Preferential accumulation of Pma1p in mother cells, which reduces the level of cytoplasmic protons, is thought to contribute to this pH increase. However, this study found that the accumulation of Pma1p in mother cells is not the key determinant of aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jovian Lin Jing, Trishia Cheng Yi Ning, Federica Natali, Frank Eisenhaber, Mohammad Alfatah
Summary: Aging is a global challenge, but iron supplementation can delay aging and increase cellular lifespan by upregulating mitochondrial genes and increasing ATP levels. Additionally, iron supplementation has anti-aging effects in AMPK knockout mutants. Thus, iron supplementation has potential therapeutic applications for delaying aging and prolonging healthy lifespan.
Article
Biochemistry & Molecular Biology
Elisa Enriquez-Hesles, Daniel L. Smith, Nazif Maqani, Margaret B. Wierman, Matthew D. Sutcliffe, Ryan D. Fine, Agata Kalita, Sean M. Santos, Michael J. Muehlbauer, James R. Bain, Kevin A. Janes, John L. Hartman, Matthew D. Hirschey, Jeffrey S. Smith
Summary: Caloric restriction has been shown to improve the health span and life span of various organisms by influencing the metabolism of specific amino acids, such as L-serine, through the one-carbon metabolism pathway. This mechanism suggests a potential non-cell-autonomous regulation of life span, providing insights into interventions for aging-associated diseases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Chenglong Zhang, Wang Chen, Tianyu Dong, Ying Wang, Mingdong Yao, Wenhai Xiao, Bingzhi Li
Summary: This study overcame the catalysis compartmentalization between two key exogenous enzymes by altering their subcellular localization, resulting in increased taxadiene yield. The highest reported titer of taxadiene biosynthesis in eukaryotic microbes was achieved.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Cristiana Garofalo, Riccardo Sabbatini, Federica Zamporlini, Gabriele Minazzato, Ilario Ferrocino, Lucia Aquilanti, Nadia Raffaelli, Andrea Osimani
Summary: This study quantified NR, NMN, and NAD+ in commercial craft beers and during fermentation, finding that adding hops significantly increased NR production. The findings serve as a baseline for further research on the interaction between yeast and hops.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xinli Li, Yue Tang, Yong Ding, Pengwei Li, Yihua Chen
Summary: This study discovered that the de novo NAD(+) biosynthetic pathway can be replaced by an artificial pathway in yeast, which opens up possibilities for designing more promising schemes to manipulate cellular NAD(H) levels in eukaryotes.
FERMENTATION-BASEL
(2023)
Article
Cell Biology
Takafumi Ogawa, Koji Masumura, Yuki Kohara, Muneyoshi Kanai, Tomoyoshi Soga, Yoshikazu Ohya, T. Keith Blackwell, Masaki Mizunuma
Summary: Methionine restriction can extend lifespan and delay aging-associated pathologies. Supplementation with S-adenosyl-L-homocysteine (SAH) can mimic the effects of methionine restriction by reducing methionine levels and activating AMP-activated protein kinase (AMPK), thereby extending lifespan.
Article
Biochemistry & Molecular Biology
Siming Wang, Juhui Qiao, Chunyan Jiang, Daian Pan, Shiting Yu, Jingjing Chen, Shichao Liu, Peiguang Zhang, Daqing Zhao, Meichen Liu
Summary: Ginsenosides, active substances in ginseng, have been found to extend lifespan, ameliorate age-related damage, and limit functional decline. However, the specific components and molecular mechanisms of ginsenosides are not well understood. In this study, ginsenoside Rg1 was found to promote longevity in Saccharomyces cerevisiae by reducing aging-related surface wrinkling, enhancing stress resistance, decreasing reactive oxygen species' production and apoptosis, improving antioxidant enzyme activity, and slowing down the aging rate. Proteomic analysis revealed that Rg1 delays yeast senescence by regulating metabolic homeostasis, with CDC19 and SDH2 potentially being the effector proteins involved. Further experiments confirmed that Rg1 improves mitochondrial bioenergetics and glycolysis in yeast. These findings suggest that Rg1 could be used as a new treatment for slowing down the aging process.
Article
Agriculture, Multidisciplinary
Chengcheng Sun, Guangjian Li, Hongbiao Li, Yunbin Lyu, Shiqin Yu, Jingwen Zhou
Summary: This study successfully achieved de novo production of two basic flavan-3-ols by engineering Saccharomyces cerevisiae, demonstrating the potential of engineering strategies in increasing yield.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)