Article
Plant Sciences
Lilei Zheng, Bingling Qiu, Linlin Su, Hanlin Wang, Xiuming Cui, Feng Ge, Diqiu Liu
Summary: In this study, 30 WRKY transcription factors (TFs) were isolated from Panax notoginseng, with PnWRKY9 identified as a key player in enhancing plant defenses against the root rot pathogen Fusarium solani. The study also revealed that PnWRKY9 positively regulates the expression of a defensin gene related to F. solani resistance through the JA signaling pathway, providing insight into the regulatory mechanism of WRKY TFs in P. notoginseng responses to root rot.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Lianxiang Long, Lijiao Gu, Shijie Wang, Hongyu Cai, Jianghao Wu, Jinmao Wang, Minsheng Yang
Summary: The WRKY transcription factor (TF) family, known for its WRKY domain, is one of the largest and most functionally diverse TF families in higher plants. WRKY TFs interact with target gene promoters and play important roles in regulating various physiological responses in woody plants. This review article discusses the origin, distribution, structure, and classification of WRKY TFs, as well as their mechanisms of action, regulatory networks, and biological functions in woody plants. It also explores current research methods, identifies outstanding problems, and proposes new research directions to further our understanding of the biological functions of WRKY TFs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Horticulture
Yaqiong Wu, Shanshan Zhang, Xin Huang, Lianfei Lyu, Weilin Li, Wenlong Wu
Summary: This study identified and analyzed the WRKY gene family in black raspberry and classified them into three groups through phylogenetic analysis. The conserved motif analysis revealed three core conserved domains in this gene family. Gene expression analysis showed that 12 WRKY genes were highly expressed in black fruits and leaves. Furthermore, the expression difference at different treatment times indicated that the response of most WRKY transcription factors to NaCl and ABA treatment in black raspberry is temporal.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Linlin Su, Lilei Zheng, Hanlin Wang, Yuan Qu, Feng Ge, Diqiu Liu
Summary: The transcription factor PnWRKY15 regulates the resistance of Panax notoginseng to Fusarium solani by up-regulating the expression of the resistance-related gene PnOLP1 and activating the JA/SA signaling pathways. Suppressed expression of PnWRKY15 increases the sensitivity of P. notoginseng to F. solani, while ectopic expression of PnWRKY15 enhances resistance in tobacco. These findings provide insights into the transcriptional regulatory mechanism of P. notoginseng defense response to F. solani.
Article
Plant Sciences
Laura C. Terron-Cameron, M. Angeles Pelaez-Vico, A. Rodriguez-Gonzalez, Coral del Val, Luisa M. Sandalio, Maria C. Romero-Puertasl
Summary: Reactive oxygen species (ROS) act as secondary messengers that activate signal transduction and alter gene expression. The chemical identity and subcellular origin of ROS determine the transcriptome response. Transcriptomic studies on altered ROS metabolism in plant peroxisomes reveal common transcriptional footprints involved in metabolic pathways, gene regulation, and plant responses to abiotic stresses. Human orthologs of peroxisomal-dependent proteins are associated with cancer pathologies. Peroxisomal footprints provide valuable insights into peroxisomal functions in cellular metabolism under normal and stress conditions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Omar A. Hewedy, Nabil I. Elsheery, Ali M. Karkour, Neveen Elhamouly, Ramadan A. Arafa, Ghada Abd-Elmonsef Mahmoud, Mona F. -A. Dawood, Walaa E. Hussein, Abdelaziz Mansour, Dina H. Amin, Suleyman I. Allakhverdiev, Marek Zivcak, Marian Brestic
Summary: Jasmonic acid and methyl jasmonate are master regulators of plant development and stress response, promoting defense interactions, regulating stomatal openness, and improving plant tolerance to abiotic stress. They also modulate plant growth and development by interacting with other plant hormones and defending against pathogen attacks.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Saima Aslam, Nadia Gul, Mudasir A. Mir, Mohd Asgher, Nadiah Al-Sulami, Aala A. Abulfaraj, Sameer Qari
Summary: Plant growth regulators play a crucial role in plant growth, particularly in response to abiotic stress. Jasmonates, calcium, and glutathione are key players in abiotic stress tolerance, with a possible novel interconnected signaling cascade between them.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Zainab Mirza, Mohammad Mahfuzul Haque, Meetu Gupta
Summary: Arsenic is a global carcinogenic contaminant that limits the development and yield of crop plants. It is closely associated with rice and accumulates in grains, posing a health risk to humans. WRKY transcription factors show promise in addressing arsenic-related issues in rice, but further research is needed.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Agronomy
Jieun Seo, Jeong Gu Lee, Byoung-Cheorl Kang, Sooyeon Lim, Eun Jin Lee
Summary: This study investigated the relationship between hormone levels, gene expression, and chilling injury in pepper fruit. 'TK' genotype was less sensitive to low temperature, while 'GG' genotype showed higher sensitivity, and GABA and CaARF19 were possibly associated with chilling injury levels.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Genetics & Heredity
Renjun Qu, Yiwen Cao, Xiaoqing Tang, Liqiong Sun, Lian Wei, Kangcai Wang
Summary: WRKY proteins play key roles in regulating growth, development, and stress responses in plants. In this study, 64 IiWRKY genes were identified in Isatis indigotica, classified into three groups based on structure and phylogenetic relationships, and found to contain two variants of the WRKYGQK motif. Gene duplication events and distinct expression patterns in different organs and stress responses were also analyzed. These findings contribute to the understanding of stress responses in this important herb.
Article
Biochemistry & Molecular Biology
Xiang-Ying Xiang, Jia Chen, Wen-Xin Xu, Jia-Rui Qiu, Li Song, Jia-Tong Wang, Rong Tang, Duoer Chen, Cai-Zhong Jiang, Zhuo Huang
Summary: The study revealed that MfWRKY70 plays a crucial role in enhancing drought, osmotic, and salinity stress tolerance in Arabidopsis plants by promoting root growth, water retention, and antioxidant enzyme system, as well as maintaining ROS homeostasis and membrane-lipid stability under stress conditions. Additionally, the overexpression of MfWRKY70 positively regulated the expression of stress-associated genes in Arabidopsis, suggesting that MfWRKY70 may act as a potential gene for improving drought and salinity tolerance in plants.
Article
Plant Sciences
Weihuang Wu, Sheng Zhu, Lin Xu, Liming Zhu, Dandan Wang, Yang Liu, Siqin Liu, Zhaodong Hao, Ye Lu, Liming Yang, Jisen Shi, Jinhui Chen
Summary: This study conducted a genome-wide analysis of the WRKY gene family in Liriodendron chinense and found that several WRKY genes respond to cold, heat, or drought stress. The LchiWRKY gene family was divided into three phylogenetic groups, and group II was further divided into five subgroups. The WRKY genes were evenly distributed across 15 chromosomes and showed evidence of tandem duplication. The genes mainly underwent purifying selection and were enriched in certain biological processes and molecular functions. Promoter cis-element analysis revealed their role in environmental adaptability. The study provides important insights into the abiotic stress response of L. chinense and can inform afforestation efforts in China.
Article
Plant Sciences
Ziyun Wan, Xueqin Li, Hefeng Cheng, Jing Zhang, Yujia Chen, Yanxia Xu, Songheng Jin
Summary: This study identified 57 RsWRKYs distributed on 13 chromosomes, divided into three groups based on gene structure and phylogenetic relationships. The highly conserved domain WRKYGQK with three variants was observed in RsWRKYs. The expression patterns of most RsWRKY genes responded to multiple phytohormones and abiotic stressors, providing a foundation for breeding stress-tolerant varieties of Rhododendron.
Article
Biochemistry & Molecular Biology
Ajay Kumar Singh, Susheel Kumar Raina, Mahesh Kumar, Lalitkumar Aher, Milind B. Ratnaparkhe, Jagadish Rane, Aardra Kachroo
Summary: This study aimed to enhance resilience of soybean crops to drought and salinity stresses by overexpressing the GmFAD3A gene. Overexpression of GmFAD3A resulted in various positive physiological responses, such as increased chlorophyll content, improved photosynthesis efficiency, and lower canopy temperature. On the other hand, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.
PLANT MOLECULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Peng Liu, Xiaolei Wu, Binbin Gong, Guiyun Lu, Jingrui Li, Hongbo Gao
Summary: Understanding the metabolism and regulation of reactive oxygen species (ROS) is crucial for improving plant growth and developing stress-tolerant cultivars. This review summarizes current research on ROS generation and scavenging in plants, as well as recent progress in transcription factor-mediated regulation of ROS metabolism. It also discusses the effects of applying exogenous substances on ROS metabolism under abiotic stress.
Article
Biochemical Research Methods
Kenneth A. Watanabe, Kaiwang Ma, Arielle Homayouni, Paul J. Rushton, Qingxi J. Shen
Article
Multidisciplinary Sciences
Prateek Tripathi, Marcela Carvallo, Elizabeth E. Hamilton, Sasha Preuss, Steve A. Kay
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biotechnology & Applied Microbiology
Roel C. Rabara, Prateek Tripathi, Paul J. Rushton
BIOMED RESEARCH INTERNATIONAL
(2017)
Article
Plant Sciences
Roel C. Rabara, Glenn Behrman, Thomas Timbol, Paul J. Rushton
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Multidisciplinary Sciences
Prateek Tripathi, Jose L. Pruneda-Paz, Steve A. Kay
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Article
Plant Sciences
Supratim Basu, Roel C. Rabara, Sangeeta Negi
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2018)
Editorial Material
Biotechnology & Applied Microbiology
Supratim Basu, Roel C. Rabara, Sangeeta Negi, Pratyoosh Shukla
TRENDS IN BIOTECHNOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Shabir H. Wani, Prateek Tripathi, Abbu Zaid, Ghana S. Challa, Anuj Kumar, Vinay Kumar, Jyoti Upadhyay, Rohit Joshi, Manoj Bhatt
PLANT MOLECULAR BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Richa Srivastava, Sanjeev Kumar, Yasufumi Kobayashi, Kazutaka Kusunoki, Prateek Tripathi, Yuriko Kobayashi, Hiroyuki Koyama, Lingaraj Sahoo
SCIENTIFIC REPORTS
(2018)
Review
Plant Sciences
Muntazir Mushtaq, Aafreen Sakina, Shabir Hussain Wani, Asif B. Shikari, Prateek Tripathi, Abbu Zaid, Aravind Galla, Mostafa Abdelrahman, Manmohan Sharma, Anil Kumar Singh, Romesh Kumar Salgotra
FRONTIERS IN PLANT SCIENCE
(2019)
Review
Biology
Shabir Hussain Wani, Vinay Kumar, Tushar Khare, Prateek Tripathi, Tariq Shah, Chopperla Ramakrishna, Supriya Aglawe, Satendra Kumar Mangrauthia
Article
Plant Sciences
Roel C. Rabara, Joseph Msanne, Supratim Basu, Marilyn C. Ferrer, Aryadeep Roychoudhury
Summary: This review highlights the impact of climate fluctuations, temperature extremes, and water scarcity on rice production, emphasizing heat and drought as crucial stressors. It examines the physiological and molecular mechanisms of rice in response to drought, high temperature stress, and their combination, as well as the genotypic differences in tolerance. Studies on phenotypic traits, genetic loci, and response mechanisms to stress conditions provide insights for developing new breeding strategies for improved abiotic stress tolerance in rice. It also discusses gene regulatory networks, transcription factors, and epigenetic regulations involved in mediating tolerance to these stresses. The review advocates for synergistic approaches in molecular and systems biology to develop new rice breeds capable of responding favorably to combined abiotic stressors induced by drought and high temperature.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
