Review
Cell Biology
Hylane Luiz Damascena, Wendy Ann Assis Silveira, Mariana S. Castro, Wagner Fontes
Summary: This review provides a brief overview of the major signaling pathways in PMA-activated neutrophils and proposes new insights on their interpretation.
Article
Biology
Lauren L. Ozimski, Maria Sabater-Arcis, Ariadna Bargiela, Ruben Artero
Summary: Myotonic dystrophy type 1 (DM1) is the most prevalent form of muscular dystrophy in adults with no current treatment options. The disease is mainly characterised by muscle pathology and affects patients worldwide. Research into various pathways affected in DM1 may provide new therapeutic targets.
BIOLOGICAL REVIEWS
(2021)
Article
Medicine, Research & Experimental
Emily E. Davey, Cecilia Legare, Lori Planco, Sharon Shaughnessy, Claudia D. Lennon, Marie-Pier Roussel, Hannah K. Shorrock, Man Hung, John Douglas Cleary, Elise Duchesne, J. Andrew Berglund
Summary: Myotonic dystrophy type 1 (DM1), caused by a CTG expansion, leads to transcriptomic dysregulation and muscle weakness. This study investigated the effects of a strength-training program on gene expression and alternative splicing in male DM1 patients. The results showed that training rescued transcriptomic defects in a patient-specific manner, and gene expression improvements were correlated with strength improvements.
Article
Immunology
Sonia Giambelluca, Matthias Ochs, Elena Lopez-Rodriguez
Summary: THP-1 monocytes can be differentiated to macrophages by incubation with PMA, and a 24-hour rest period allows the down-regulation of pro-IL-1 beta without affecting the ability to respond to a second stimulus through activation of the inflammasome.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jorge Espinosa-Espinosa, Anchel Gonzalez-Barriga, Arturo Lopez-Castel, Ruben Artero
Summary: Omics studies are crucial for understanding DM1, revealing various changes in gene and microRNA expression, alternative splicing, 3 ' polyadenylation, CpG methylation, and protein levels. Moreover, omics characterization of drug treatment experiments provides insights into therapeutic rescue and off-target effects. Innovative technologies like single-cell sequencing and AI will have a significant impact on future DM1 research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ryu Kawada, Tatsuya Jonouchi, Akihiro Kagita, Masae Sato, Akitsu Hotta, Hidetoshi Sakurai
Summary: This study established in vitro skeletal muscle cell models of DM1 using patient-derived iPSCs. Quantitative analyses showed that CTGexp-deleted DM1 myotubes reversed MBNL1-related pathologies, and oligonucleotide treatment recovered disease phenotypes. The models are promising for drug discovery against DM1.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ya-Ying Chang, Cheng-Wei Lu, Wei-Horng Jean, Jiann-Shing Shieh, Tzu-Yu Lin
Summary: The study revealed that the effects of PMA on THP-1 cells' adherence, cell function, and migration ability were closely related to NO concentrations, while calcium signaling may mediate the PMA-induced upregulation of NO.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Hannah Tovell, Alexandra C. Newton
Summary: Protein kinase signalling is often disrupted in human diseases, particularly in cancer. While 77 kinase inhibitors are currently approved for treatment by the FDA, targeting PKC as an oncogene in cancer has not been successful. Recent studies suggest that restoring PKC function may be a more effective approach, as high PKC expression is protective in many cancers.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Kyung-Ho Jung, Jin Hee Lee, Mina Kim, Young Seok Cho, Kyung-Han Lee
Summary: An immuno-PET technique was developed to quantitatively image and monitor the regulation of PD-1 expression on T-cell lymphomas. Results showed that Zr-89-PD-1 IgG could effectively bind to EL4 cells and be used for PET imaging of PD-1 in mice. The expression of PD-1 on T-cell lymphomas was upregulated by PMA stimulation, and this could be reliably monitored by Zr-89-PD-1 IgG PET.
Article
Integrative & Complementary Medicine
Yoon-Jong Park, Jae-Min Ryu, Han-Heom Na, Hyun-Suk Jung, Bokhye Kim, Jin-Sung Park, Byung-Soo Ahn, Keun-Cheol Kim
Summary: This study investigated the expression and regulatory mechanism of beta-catenin by CBD in alopecia hair tissues and dermal papilla cells. It was found that beta-catenin expression plays a key role in regulating alopecia progression. CBD may have a modulating effect on alopecia caused by hormonal factors or excess signaling pathways.
JOURNAL OF PHARMACOPUNCTURE
(2021)
Article
Biochemistry & Molecular Biology
Takao Hikita, Fatemeh Mirzapourshafiyi, Pedro Barbacena, Meghan Riddell, Ayesha Pasha, Mengnan Li, Takuji Kawamura, Ralf P. Brandes, Tomonori Hirose, Shigeo Ohno, Holger Gerhardt, Michiyuki Matsuda, Claudio A. Franco, Masanori Nakayama
Article
Obstetrics & Gynecology
Aya Tokinaga-Uchiyama, Taichi Mizushima, Kazunori Akimoto, Yoji Nagashima, Kazunori Sasaki, Masa-aki Nakaya, Kenichi Ohashi, Kazumi Kubota, Yasuyo Maruyama, Hisamori Kato, Fumiki Hirahara, Etsuko Miyagi, Shigeo Ohno, Mikiko Asai-Sato
INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
(2019)
Article
Oncology
Junichi Baba, Mitomu Kioi, Kazunori Akimoto, Yoji Nagashima, Masataka Taguri, Yoshiaki Inayama, Ichiro Aoki, Shigeo Ohno, Kenji Mitsudo, Iwai Tohnai
ANTICANCER RESEARCH
(2018)
Article
Dermatology
Natsuko Noguchi, Tomonori Hirose, Tomoko Suzuki, Masami Kagaya, Kazuhiro Chida, Shigeo Ohno, Motomu Manabe, Shin-Ichi Osada
JOURNAL OF DERMATOLOGICAL SCIENCE
(2019)
Article
Multidisciplinary Sciences
Meghan Riddell, Akiko Nakayama, Takao Hikita, Fatemeh Mirzapourshafiyi, Takuji Kawamura, Ayesha Pasha, Mengnan Li, Mikio Masuzawa, Mario Looso, Tim Steinbacher, Klaus Ebnet, Michael Potente, Tomonori Hirose, Shigeo Ohno, Ingrid Fleming, Stefan Gattenloehner, Phyu P. Aung, Thuy Phung, Osamu Yamasaki, Teruki Yanagi, Hiroshi Umemura, Masanori Nakayama
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Takashi Mino, Noriki Iwai, Masayuki Endo, Kentaro Inoue, Kotaro Akaki, Fabian Hia, Takuya Uehata, Tomoko Emura, Kumi Hidaka, Yutaka Suzuki, Daron M. Standley, Mariko Okada-Hatakeyama, Shigeo Ohno, Hiroshi Sugiyama, Akio Yamashita, Osamu Takeuchi
NUCLEIC ACIDS RESEARCH
(2019)
Article
Neurosciences
Yanyan Jia, Yimeng Zhao, Tsukasa Kusakizako, Yao Wang, Chengfang Pan, Yuwei Zhang, Osamu Nureki, Motoyuki Hattori, Zhiqiang Yan
Article
Cell Biology
Kazunori Sasaki, Noriko Kojitani, Hiroko Hirose, Yohei Yoshihama, Hidefumi Suzuki, Miho Shimada, Ayumi Takayanagi, Akio Yamashita, Masa-aki Nakaya, Hisashi Hirano, Hidehisa Takahashi, Shigeo Ohno
Article
Multidisciplinary Sciences
Faji Cai, Yichen Huang, Mengqi Wang, Minxuan Sun, Yimeng Zhao, Motoyuki Hattori
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Kazunari Yamashita, Keiko Mizuno, Kana Furukawa, Hiroko Hirose, Natsuki Sakurai, Maki Masuda-Hirata, Yoshiko Amano, Tomonori Hirose, Atsushi Suzuki, Shigeo Ohno
JOURNAL OF CELL SCIENCE
(2020)
Article
Developmental Biology
Tomonori Hirose, Yoshinobu Sugitani, Hidetake Kurihara, Hiromi Kazama, Chiho Kusaka, Tetsuo Noda, Hidehisa Takahashi, Shigeo Ohno
Summary: During brain development, PAR3 plays a crucial role in the transition of neural precursor cells from the expansion phase to the neurogenic phase by restricting hedgehog signaling through the establishment of ciliary integrity.
Article
Multidisciplinary Sciences
Ayumi Sumino, Yimeng Zhao, Daichi Mukai, Takashi Sumikama, Leonardo Puppulin, Motoyuki Hattori, Mikihiro Shibata
Summary: The study directly visualized the single-molecule structural fluctuations of TRPV1 channels using high-speed atomic force microscopy. It found that the binding of resiniferatoxin (RTX) enhances structural fluctuations, while the binding of capsazepine (CPZ) suppresses fluctuations. These ligand-dependent differences in structural fluctuation are important for the gating of TRPV1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Takahiro Kasai, Shoma Tamori, Yuta Takasaki, Izumi Matsuoka, Ayaka Ozaki, Chika Matsuda, Yohsuke Harada, Kazunori Sasaki, Shigeo Ohno, Kazunori Akimoto
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly heterogeneous cancer with poor prognosis, which leads to insensitivity to anticancer treatments. Cancer stem cells (CSCs) acquire phenotypic heterogeneity through asymmetric cell division, but the detailed mechanism is largely unknown.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Meeting Abstract
Developmental Biology
Kana Tamura-Furukawa, Kazunari Yamashita, Shigeo Ohno
Meeting Abstract
Cell Biology
K. Yamashita, K. T. Furukawa, N. Sakurai, S. Ohno
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)