期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 411, 期 3, 页码 613-619出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.07.002
关键词
Hot pepper (Capsicum annuum L.); CaPR-10 promoter; WRKY transcription factor; VIGS; Tobacco mosaic virus
资金
- Ministry of Education. Science and Technology [R11-2003-008-02001-0, 2009-0085565]
- Korea University
- Rural Development Administration, Republic of Korea [PJ007850]
- National Research Foundation of Korea [2009-0085565, R11-2003-008-02001-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In plant, some WRKY transcription factors are known to play an important role in the transcriptional reprogramming associated with the immune response. By using WRKY-domain-specific differential display procedure, we isolated CaWRKYb gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) pathotype P(0) infection. The recombinant CaWRKYb bound to the W box-containing CaPR-10 promoter probes efficiently and the specificity of binding was confirmed by mutant study and competition with cold oligonucleotides. Also, in GUS reporter activity assay using Arabidopsis protoplasts with the CaPR-10 promoter, GUS activity was increased in the presence of CaWRKYb. And CaWRKYb-knockdown plant showed reduced number of hypersensitive response local lesions upon TMV-P(0) infection. Furthermore, CaWRKYb-knockdown plant exhibited compromised resistance to TMV-P(0) by accumulating more TMV, apparently through decreased expression of CaPR10, CaPR-1, and CaPR-5. These results suggest that CaWRICYb is involved as a positive transcription factor in defense-related signal transduction pathways in hot pepper. (C) 2011 Elsevier Inc. All rights reserved.
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