Review
Pharmacology & Pharmacy
Jennifer Leigh Green, Wagner Ferreira dos Santos, Andreia Cristina Karklin Fontana
Summary: Epilepsy is a complex neurological syndrome characterized by seizures resulting from neuronal hyperexcitability, with glutamate excitotoxicity playing a key role in the pathogenesis. Targeting EAAT2, the predominant glutamate transporter in the CNS, through upregulation or positive allosteric modulation, offers promising approaches for reducing seizures and providing neuroprotective effects in epilepsy therapy. Novel EAAT2 PAMs under development represent a hopeful avenue for advancing novel therapeutics for epilepsy.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Neurosciences
Xingxing Xu, Jiaojiao Wang, Siyu Du, Xiya Shen, Jiashu Lian, Jian Zhou, Mianxian Wang, Wenjin Feng, Zhaoting Lv, Junzhe Zhu, Lingting Jin, Huankun Sun, Lihao Wu, Xiaoning Wang, Haoyu Qiu, Wei Wang, Honglin Teng, Ying Wang, Zhihui Huang
Summary: Our study reveals that YAP signaling plays an important role in the regulation of glutamate homeostasis through the beta-catenin/EAAT2 pathway in astrocytes. This finding provides new insights into the pathogenesis of brain diseases related to EAAT2 dysfunction or dysregulation and may contribute to the development of clinical strategies.
Review
Biochemistry & Molecular Biology
Areeba Nisar, Zubair Ahmed, Hsiangkuo Yuan
Summary: Migraine is a debilitating neurological condition that affects many patients' quality of life. Despite the success of new therapies, not all patients benefit significantly. Understanding the mechanisms involved in migraine can lead to the discovery of novel therapeutic targets for improved treatment options.
Article
Agriculture, Multidisciplinary
Zhiguo Liu, Ye Yuan, Lili Wang, Xuan Zhao, Lixin Wang, Lihu Wang, Zhihui Zhao, Xin Zhao, Yuetong Chu, Yaning Gao, Fangyuan Yang, Yulu Wang, Qiong Zhang, Jin Zhao, Mengjun Liu
Summary: This study systematically investigated plant adenylate cyclases using Chinese jujube, a plant with high cAMP content. Three novel ACs were identified and their functions were confirmed using in vitro and in vivo methods. Overexpression of these AC genes in Arabidopsis resulted in accelerated seed germination, root growth, and flowering. Transcriptome data suggested that the biological functions of ACs may be mediated through the regulation of key genes involved in the circadian rhythm pathway and hormone signal transduction pathway. This research lays the foundation for further studying plant AC genes and provides strong evidence for cAMP serving as a signaling molecule in plants.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Rhea Temmermand, James E. Barrett, Andreia C. K. Fontana
Summary: Neuropathic pain is a common disease that lacks effective pharmacotherapies. Modulating glutamate transporters may be a novel approach to treating pain. This review summarizes the physiology of the glutamatergic system, preclinical evidence in animal models, and emerging therapies that modulate glutamate transport in neuropathic pain.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Jeroen Spanoghe, Lars E. Larsen, Erine Craey, Simona Manzella, Annelies Van Dycke, Paul Boon, Robrecht Raedt
Summary: Activation of adenosine A(1) receptor can inhibit seizures through various signaling pathways leading to neuronal inhibition. However, chronic activation of the A(1) receptor may have detrimental effects that should be avoided in the development of epilepsy therapies based on this receptor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Sonia Munoz-Lopez, Alejandro Sanchez-Melgar, Mairena Martin, Jose Luis Albasanz
Summary: Despite the lack of effective treatment for cancer, natural compounds such as resveratrol have shown promise in cancer therapy. Resveratrol can modulate various signaling pathways related to cancer initiation and progression. This study investigates the impact of resveratrol treatment on adenosinergic pathway components and cell viability in HeLa and SH-SY5Y cell lines, revealing changes in the pathway that may contribute to the anti-tumoral effects of resveratrol.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Ya Berzina, Barbara Z. Eletskaya, Alexei L. Kayushin, Elena Dorofeeva, Olga Lutonina, Ilya Fateev, Alexander S. Paramonov, Maria A. Kostromina, Evgeniy A. Zayats, Yulia A. Abramchik, Dmitriy Maltsev, Ludmila Naumenko, Alena S. Taran, Dmitry S. Yakovlev, Alexander A. Spasov, Anatoly Miroshnikov, Roman S. Esipov, Irina D. Konstantinova
Summary: A series of purine ribonucleosides with chiral amino acid amides were synthesized and their affinity for A(1) adenosine receptors (A(1)ARs) was investigated. It was found that some of the synthesized nucleosides exhibited partial agonist activity towards A(1)ARs and showed different profiles of psychoactive action and ocular hypotensive effect.
BIOORGANIC CHEMISTRY
(2022)
Article
Biology
Piotr Michaluk, Janosch Peter Heller, Dmitri A. Rusakov
Summary: By generating its pH-sensitive fluorescent analogue GLT1-SEP, researchers visualized the dynamics of the main glial glutamate transporter GLT1 in astrocyte membrane, revealing its surface dwell time and turnover pattern. The results show that membrane turnover is the main 'redeployment' route for the immobile fraction of surface-expressed GLT1, helping to control extrasynaptic escape of glutamate.
Article
Neurosciences
Masayoshi Tanaka, Eiji Shigetomi, Bijay Parajuli, Hiroaki Nagatomo, Youichi Shinozaki, Yuri Hirayama, Kozo Saito, Yuto Kubota, Yosuke Danjo, Ji Hwan Lee, Sun Kwang Kim, Junichi Nabekura, Schuichi Koizumi
Summary: mGluR5 in astrocytes is regulated by ATP/adenosine signals, which helps control synapse remodeling and potentially contributes to disease pathogenesis.
Article
Neurosciences
Bradley M. Roberts, Elizabeth Lambert, Jessica A. Livesey, Zhaofa Wu, Yulong Li, Stephanie J. Cragg
Summary: Activation of Y striatal adenosine A1 receptors can inhibit dopamine release, with this inhibition modulated by astrocytic ENT1 and ethanol. The release of dopamine in the nucleus accumbens core is under tonic inhibition by A1Rs and this process is regulated by adenosine tone in the striatum, suggesting a complex interplay between neurotransmitters and astrocytic transporters in the regulation of dopamine dynamics.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zachary Carlson, Kelly Drew
Summary: Hibernation is an adaptation that allows animals to survive without food or water. Understanding the mechanisms of metabolic suppression during hibernation could lead to new treatments in critical care. The activation of Adenosine A(1) receptor (A(1)AR) plays a role in hibernation, but its role in seasonal sensitization is unknown. In this study, the A(1)AR in different brain regions of Arctic ground squirrels during summer and torpor seasons was characterized, and the pharmacological characteristics of A(1)AR agonist and antagonist were defined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Linlin Bi, Yang Liu, Qian Yang, Xuanxuan Zhou, Hua Li, Jie Li, Yunyang Lu, Haielng Tang
Summary: The study investigated the effects of Paris saponin H (PSH) on U251 glioblastoma cells and found that PSH inhibited cell viability, migration, invasion, induced apoptosis, and cell cycle arrest by inhibiting ARA1 and ARA3 expression, repressing Akt and 44/42 MAPK phosphorylation.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Endocrinology & Metabolism
Mitchell E. Granade, Stefan R. Hargett, Daniel S. Lank, Michael C. Lemke, Melissa A. Luse, Brant E. Isakson, Irina M. Bochkis, Joel Linden, Thurl E. Harris
Summary: Adipose tissue response to adenosine changes dynamically according to nutrient conditions through the insulin-Akt-FOXO1 axis. Deletion of adipocyte A1R leads to impairments in lipolysis suppression by insulin under fasting conditions and compromised glucose tolerance. Refeeding results in downregulation of A1R transcription and desensitization of adipocytes to A1R agonism, facilitating reentrance into the catabolic state upon fasting.
MOLECULAR METABOLISM
(2022)
Article
Endocrinology & Metabolism
Mitchell E. Granade, Stefan R. Hargett, Daniel S. Lank, Michael C. Lemke, Melissa A. Luse, Brant E. Isakson, Irina M. Bochkis, Joel Linden, Thurl E. Harris
Summary: This study reveals that adenosine regulates adipocyte metabolism through A1R, with adipose tissue exhibiting dynamic responses to adenosine under fasting and feeding conditions. High A1R expression under fasting limits excessive lipolysis, while downregulation of A1R under feeding leads to desensitization of adipocytes to A1R.
MOLECULAR METABOLISM
(2022)
Editorial Material
Substance Abuse
Seungwoo Kang, Doo-Sup Choi
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Phillip Starski, Sa-Ik Hong, Lee Peyton, Alfredo Oliveros, Katheryn Wininger, Colleen Hutchison, Seungwoo Kang, Victor Karpyak, Doo-Sup Choi
Article
Substance Abuse
Daniel Lindberg, Ada Man Choi Ho, Lee Peyton, Doo-Sup Choi
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2019)
Article
Obstetrics & Gynecology
Ronald I. Clyman, Nancy K. Hills, Melissa Liebowitz, Sandy Johng
AMERICAN JOURNAL OF PERINATOLOGY
(2020)
Article
Neurosciences
Seungwoo Kang, Sa-Ik Hong, Jeyeon Lee, Lee Peyton, Matthew Baker, Sun Choi, Hyunjung Kim, Su-Youne Chang, Doo-Sup Choi
BIOLOGICAL PSYCHIATRY
(2020)
Article
Neurosciences
Sa-Ik Hong, Amanda Bullert, Matthew Baker, Doo-Sup Choi
EUROPEAN JOURNAL OF NEUROSCIENCE
(2020)
Article
Pediatrics
Sandy S. Johng, Valerie Gribben, Ellen Laves
PEDIATRICS IN REVIEW
(2020)
Article
Biochemistry & Molecular Biology
Fatimah Al Khazal, Seungwoo Kang, Molly Nelson Holte, Doo-Sup Choi, Ravinder Singh, Patricia Ortega-Saenz, Jose Lopez-Barneo, L. James Maher
Summary: Mutations in genes encoding the subunits of succinate dehydrogenase can cause various disorders, including hereditary tumors. However, loss of SDHC did not result in PPGL in mice, but instead caused non-diabetic obesity.
Review
Clinical Neurology
Pei Shang, Matthew Baker, Samantha Banks, Sa-Ik Hong, Doo-Sup Chol
Summary: A(2A)R antagonists and GLP1R agonists may have potential roles in the management of Parkinson's disease, with the former reducing dyskinesia and the latter alleviating neuroinflammation and sustaining cellular functions in the central nervous system.
JOURNAL OF MOVEMENT DISORDERS
(2021)
Article
Neurosciences
Lee Peyton, Brandon Emanuel Leon, Hesham Essa, Yijuang Chern, Doo-Sup Choi
Summary: This study investigates the effects of J4, an adenosine analog, on alleviating alcohol withdrawal-related affective behaviors. The results show that J4 treatment reduces anxiety-like behavior and increases time spent in open arms, suggesting its potential as a general anxiolytic.
PSYCHOPHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ada Man-Choi Ho, Mina P. Peyton, Samantha J. Scaletty, Sarah Trapp, Areonna Schreiber, Benjamin J. Madden, Doo-Sup Choi, Douglas B. Matthews
Summary: Repeated excessive alcohol consumption increases the risk of cognitive decline and dementia in older adults. This study found that chronic intermittent ethanol exposure impaired behavioral flexibility in aged rats but not in young adult rats. Proteomic analysis revealed alcohol treatment-related changes in proteins and pathways associated with neural transmission, synaptic plasticity, neuronal apoptosis, and insulin receptor signaling. These findings contribute to understanding the detrimental effects of alcohol on cognitive function in older adults.
Article
Neurosciences
Brandon Emanuel Leon, Lee Peyton, Hesham Essa, Tia Wieden, Nicole Marion, Wayne E. Childers, Magid Abou-Gharbia, Doo-Sup Choi
Summary: The study found that MC-100093 treatment has positive effects on depressive behaviors and ethanol consumption in female mice, but no significant effects in male mice. In addition, MC-100093 treatment reduces female preference for 10% ethanol. Western blot analysis of the medial prefrontal cortex and hippocampus revealed no changes in GLT1 protein abundance in both male and female mice.
Article
Multidisciplinary Sciences
Matthew Baker, Seungwoo Kang, Sa-Ik Hong, Minryung Song, Minsu Abel Yang, Lee Peyton, Hesham Essa, Sang Wan Lee, Doo-Sup Choi
Summary: The authors found that GPe arky neurons are involved in reward-seeking behaviors and primarily project to the dorsolateral striatum. Genetic ablation of this circuit shift behavior from goal-directed to habitual seeking.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Vicki Wang, Tung-Tai Kuo, Eagle Yi-Kung Huang, Kuo-Hsing Ma, Yu-Ching Chou, Zhao-Yang Fu, Li-Wen Lai, Jin Jung, Hoi- Choi, Doo-Sup Choi, Yazhou Li, Lars Olson, Nigel H. Greig, Barry J. Hoffer, Yuan-Hao Chen
Summary: GLP-1 agonists, such as PT320, show potential in improving behavioral and cellular parameters in a mouse model of Parkinson's disease (PD), particularly in the early stages of the disease. The efficacy may be closely related to enhanced dopamine midbrain function.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2021)
Article
Neurosciences
Lee Peyton, Alfredo Oliveros, Maximilian Tufvesson-Alm, Lilly Schwieler, Phillip Starski, Goran Engberg, Sopie Erhardt, Doo-Sup Choi
INTERNATIONAL JOURNAL OF TRYPTOPHAN RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)