Article
Cell Biology
Mohamed Chamlali, Sana Kouba, Lise Rodat-Despoix, Luca Matteo Todesca, Zoltan Pethoe, Albrecht Schwab, Halima Ouadid-Ahidouch
Summary: Orai3 regulates breast cancer cell migration through a Ca2+-dependent modulation of cell adhesive capacities and a Ca2+-independent regulation of the actin cytoskeleton via focal adhesion kinase (FAK).
Article
Cell Biology
Grace Cai, Anh Nguyen, Yashar Bashirzadeh, Shan-Shan Lin, Dapeng Bi, Allen P. Liu
Summary: This study reveals the importance of cell-cell adhesion and substrate traction in cellular unjamming transitions under compressive stress. Strengthened intercellular adhesion and attenuation of traction forces contribute to the fluidization of cell layer, which may impact collective cell motion in tumor development and breast cancer progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Simone Spinozzi, Sonia Albini, Heather Best, Isabelle Richard
Summary: This review pays tribute to the late Dr. Hiroyuki Sorimachi, presenting basic information on calpains while avoiding similarities with previous reviews and highlighting Hiroyuki's contributions to the field.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Chemistry, Multidisciplinary
Alexandros Afthinos, Kaustav Bera, Junjie Chen, Altug Ozcelikkale, Alice Amitrano, Mohammad Ikbal Choudhury, Randy Huang, Pavlos Pachidis, Panagiotis Mistriotis, Yun Chen, Konstantinos Konstantopoulos
Summary: In this study, we developed polyacrylamide-based microchannels with physiological stiffness and prescribed dimensions for high-throughput analysis of cell migration. We found a biphasic dependence of cell speed on confinement and stiffness. By utilizing novel four-walled microchannels, we revealed the distinct contributions of apicolateral and basal microchannel wall stiffness to confined and unconfined migration. We also innovated three-dimensional traction force measurements around spatially confining cells at subcellular resolution by tracking nanobeads embedded within the channel walls.
Article
Multidisciplinary Sciences
Isabel M. Martin, Michele M. Nava, Sara A. Wickstroem, Frauke Graeter
Summary: This study investigates the mechanotransducing functions of the essential adhesion component integrin-linked kinase (ILK) using force-probe molecular-dynamics simulations. The results suggest that ATP plays a role in facilitating cellular force generation and migration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ke Gu, Guangmang Liu, Caimei Wu, Gang Jia, Hua Zhao, Xiaoling Chen, Gang Tian, Jingyi Cai, Ruinan Zhang, Jing Wang
Summary: This study demonstrated that tryptophan enhances cell migration in porcine intestine epithelial cells (IPEC-J2) through the CaSR/Rac1/PLC-γ1 signaling pathway.
Article
Chemistry, Multidisciplinary
Feiyu Yang, Pengcheng Chen, Han Jiang, Tianfa Xie, Yue Shao, Deok-Ho Kim, Bo Li, Yubing Sun
Summary: Strain gradients play a crucial role in directional cell migration. This study develops a programmable uniaxial cell stretch device to create controllable strain gradients and demonstrates that these gradients are sufficient to drive over 60% of rat embryonic fibroblasts towards the lower strain side. Focal adhesion analysis and a motor-clutch model suggest that strain-introduced traction force and integrin fibronectin pairs' catch-release dynamics are responsible for such directional migration.
Article
Biochemistry & Molecular Biology
Dharma Pally, Mallar Banerjee, Shahid Hussain, Rekha Kumar, Alexandra Petersson, Ebba Rosendal, Ludvig Gunnarsson, Kristoffer Peterson, Hakon Leffler, Ulf J. Nilsson, Ramray Bhat
Summary: Aberrations in glycan and lectin expression and function contribute to the development of cancer. This study focused on Galectin-9, a member of the beta-galactoside-binding lectin family, and investigated its role in breast cancer cell invasiveness. The results showed a correlation between Galectin-9 expression and the ability of invasive cancer cells to adhere and invade extracellular matrix microenvironments. Further experiments revealed that Galectin-9 induced Focal Adhesion Kinase activity and S100A4 expression, which are associated with cancer cell invasion. The findings provide crucial insights into the mechanisms by which elevated Galectin-9 expression enhances breast cancer cell invasiveness during the early stages of invasion.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Masahide Konno, Yuri Sakata, Taku Ozaki
Summary: The present study demonstrates that the activation of mitochondrial calpain-5 triggers the truncation of caspase-4, suggesting that mitochondrial calpain-5 regulates the downstream pathway of caspase-4, including cell death and the inflammatory cascade. These findings provide new insights into ER-stress-related diseases such as Alzheimer's disease and cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Xingyu Shen, Liqiu Hu, Zhen Li, Liyun Wang, Xiangchao Pang, Chun-Yi Wen, Bin Tang
Summary: The increase in extracellular calcium ion concentration can affect the elastic modulus and cell-ECM adhesion force of chondrocytes. This regulation of cell stiffness and cell-ECM adhesion may influence chondrocyte migration ability by modulating the migration speed as these factors increase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Ivan Shapovalov, Danielle Harper, Peter A. Greer
Summary: Pre-clinical and clinical studies have shown that inhibition of calpain-1 and calpain-2 has the potential to attenuate carcinogenesis and block metastasis of aggressive tumors. However, the wide range of substrates and cleavage products, as well as inconsistencies in model systems, highlight the need for a better understanding of physiological substrates and how calpain cleavage affects their functions in cellular processes. The development of isoform specific calpain inhibitors remains an important goal with therapeutic potential in cancer and other diseases.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2022)
Article
Biophysics
Mary Ellen Rosen, J. C. Dallon
Summary: By analyzing the model and experimental data of the focal adhesion lifetime distribution, it was found that cell speed is related to the mean adhesion lifetime, with longer lifetimes indicating slower speeds.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Elisabeth Ghabache, Yuansheng Cao, Yuchuan Miao, Alex Groisman, Peter N. Devreotes, Wouter-Jan Rappel
Summary: The dynamics of cytoskeletal components critically determine traction force patterns, cell morphology, and migration mode. Fan-shaped cells can exhibit two different propulsion mechanisms, each with a distinct traction force pattern.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Abdurazak Aman Ketebo, Chanyong Park, Jaewon Kim, Myeongjun Jun, Sungsu Park
Summary: The study found that Filamin A (FLNa) plays an important role in cell polarization, migration speed, and invasiveness in U87 glioma cells.
Article
Engineering, Biomedical
Hiroyuki Ebata, Satoru Kidoaki
Summary: The study found that the prolonged fluctuation of traction stress on a whole-cell scale was significantly enhanced on single cell-size triangular stiff patterns, which was derived from the interplay between the nomadic migration of cells to regions with different degrees of stiffness and domain shape-dependent traction force dynamics. This enhancement should be an essential factor for keeping cells far from tensional equilibrium.
Article
Cell Biology
Shalini Menon, Nicholas Patrick Boyer, Cortney Chelise Winkle, Leslie Marie McClain, Christopher Carey Hanlin, Dharmendra Pandey, Simon Rothenfusser, Anne Marion Taylor, Stephanie Lynn Gupton
DEVELOPMENTAL CELL
(2015)
Article
Biochemical Research Methods
A. M. Taylor, S. Menon, S. L. Gupton
Article
Cell Biology
Melissa Plooster, Shalini Menon, Cortney C. Winkle, Fabio L. Urbina, Caroline Monkiewicz, Kristen D. Phend, Richard J. Weinberg, Stephanie L. Gupton
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Multidisciplinary Sciences
Shalini Menon, Karen A. Beningo
Article
Cell Biology
Nicholas P. Boyer, Laura E. McCormick, Shalini Menon, Fabio L. Urbina, Stephanie L. Gupton
JOURNAL OF CELL BIOLOGY
(2020)
Article
Neurosciences
Nicholas P. Boyer, Caroline Monkiewicz, Shalini Menon, Sheryl S. Moy, Stephanie L. Gupton
Article
Biochemistry & Molecular Biology
Gopa R. Mishra, M. Suresh, K. Kumaran, N. Kannabiran, Shubha Suresh, P. Bala, K. Shivakumar, N. Anuradha, Raghunath Reddy, T. Madhan Raghavan, Shalini Menon, G. Hanumanthu, Malvika Gupta, Sapna Upendran, Shweta Gupta, M. Mahesh, Bincy Jacob, Pinky Mathew, Pritam Chatterjee, K. S. Arun, Salil Sharma, K. N. Chandrika, Nandan Deshpande, Kshitish Palvankar, R. Raghavnath, R. Krishnakanth, Hiren Karathia, B. Rekha, Rashmi Nayak, G. Vishnupriya, H. G. Mohan Kumar, M. Nagini, G. S. Sameer Kumar, Rojan Jose, P. Deepthi, S. Sujatha Mohan, T. K. B. Gandhi, H. C. Harsha, Krishna S. Deshpande, Malabika Sarker, T. S. Keshava Prasad, Akhilesh Pandey
NUCLEIC ACIDS RESEARCH
(2006)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)