Article
Biochemistry & Molecular Biology
Pia Maria van gen Hassend, Aparna Pottikkadavath, Carolyn Delto, Monika Kuhn, Michelle Endres, Lars Schoenemann, Hermann Schindelin
Summary: The CTLH complex is a multisubunit RING E3 ligase with flexible subunit composition and poorly defined substrate specificity. By using various biophysical and biochemical techniques, we characterized the assembly pathways of the CTLH complex, focusing on key subunits Wdr26, muskelin, RanBP9, Twa1, and Armc8 beta. Our study revealed that muskelin tetramerization and Wdr26 dimer assembly define mutually exclusive oligomerization modules that compete for binding to RanBP9. Additionally, we found that RanBP9 organizes subunit assembly and prevents higher order oligomerization of Wdr26 and the Armc8 beta-Twa1 heterodimer. These findings advance our understanding of the CTLH complex architectures.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Microbiology
Christina Wilkinson, Meredith T. Morris
Summary: This study demonstrates a complex relationship between FBPase activity and extracellular glucose levels in Trypanosoma brucei, suggesting a potential additional role for FBPase in parasite metabolism. The differential expression of FBPase in various parasite cell lines and growth conditions implies regulation via post-translational modifications. These findings provide new insights into the metabolic pathways of Trypanosoma brucei.
Article
Biochemistry & Molecular Biology
Mandy Pack, Tim Nikolai Gulde, Michelle Victoria Voelcker, Anne S. Boewe, Selina Wrublewsky, Emmanuel Ampofo, Mathias Montenarh, Claudia Goetz
Summary: Glucose homeostasis is vital for organism survival and is regulated by CK2. In this study, a qRT-PCR array was used to identify diabetes-associated genes and it was found that inhibition of CK2 led to decreased gene expression and activity of fructose-1,6-bisphosphatase 1 (FBP1), a central enzyme in gluconeogenesis. As a result, glucose secretion was reduced. These findings highlight FBP1 as a new factor in the CK2-regulated network of carbohydrate metabolism control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Masaya Tanaka, Hiroshi Endo, Konatsu Sakusa, Mihiro Yano
Summary: Hesperetin exerts its anticancer activity by inducing cell apoptosis and inhibiting cell proliferation, with Hsp70 playing a critical role in the negative regulation of this process.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Brigida R. Pinho, Liliana M. Almeida, Michael R. Duchen, Jorge M. A. Oliveira
Summary: The use of YM-1 can effectively modulate huntingtin protein proteostasis, reducing aggregation and enhancing degradation, making it a potential approach for treating Huntington's disease.
Article
Biochemistry & Molecular Biology
Areeb Jawed, Chi-Ting Ho, Tomas Grousl, Aseem Shrivastava, Thomas Ruppert, Bernd Bukau, Axel Mogk
Summary: To counteract proteotoxic stress and cellular aging, protein quality control (PQC) systems rely on the refolding, degradation and sequestration of misfolded proteins. The Hsp70 chaperone system plays a central role in protein refolding, while degradation is executed by the ubiquitin proteasome system (UPS). In yeast, sequestrase mutants show negative genetic interactions with mutants lacking the Hsp70 co-chaperone Fes1 and the Hsp104 disaggregase and suffering from low Hsp70 capacity. The enhanced activity of the UPS aggravates the growth defects of the mutant cells, while reducing UPS activity improves growth and protein refolding yield.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Genetics & Heredity
Deepika Gaur, Navinder Kumar, Abhirupa Ghosh, Prashant Singh, Pradeep Kumar, Jyoti Guleria, Satinderdeep Kaur, Nikhil Malik, Sudipto Saha, Thomas Nystrom, Deepak Sharma
Summary: Hsp70 plays a critical role in the Hsp90 chaperoning pathway. Novel mutations in the nucleotide-binding domain of yeast Ssa1 Hsp70 have been identified, which adversely affect Hsp90 client maturation. These mutations result in better binding with Ydj1 and poor growth support in the absence of Sti1. Furthermore, downregulation of pathways involved in signaling, signal transduction, and protein phosphorylation was observed in cells expressing the mutant Hsp70. The study shows that Ydj1 interaction at the nucleotide-binding domain of Ssa1 Hsp70 influences Hsp90 function.
Review
Biochemistry & Molecular Biology
Daniel Betancourt, Tomiwa Lawal, Robert J. Tomko Jr
Summary: The 26S proteasome is a large and complex protease that is implicated in numerous human diseases. The assembly of the proteasome requires the coordinated actions of intrinsic proteasome subunits and dedicated assembly chaperones. Recent advances in cryo-electron microscopy have revealed the dynamic nature of the proteasome, with conformational changes occurring during ligand binding and the proteolytic cycle. This review focuses on proteasome assembly, particularly on how conformational dynamics guide biogenesis events, and highlights key questions in this field.
Article
Cell Biology
Friederike Hantel, Huaize Liu, Lisa Fechtner, Herbert Neuhaus, Jie Ding, Danilo Arlt, Peter Walentek, Pablo Villavicencio-Lorini, Christoph Gerhardt, Thomas Hollemann, Thorsten Pfirrmann
Summary: Cilia play an important role in embryonic development, and the GID complex is involved in primary cilia-dependent signal transduction and protein homeostasis. GID subunits may be candidate genes for human ciliopathies associated with defects in Hedgehog signaling.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Aakriti Gupta, Anchal Varma, Kenneth B. Storey
Summary: The red-eared slider undergoes physiological and metabolic changes in anaerobic conditions, including the regulation of the key enzyme fructose-1,6-bisphosphatase. The activity of FBPase decreases significantly in anaerobic conditions, along with reduced sensitivity to its substrate Fructose-1,6-bisphosphate. Increased threonine phosphorylation in the anoxic FBPase suggests an important role in suppressing the enzyme's activity and inhibiting gluconeogenesis in the liver.
Article
Multidisciplinary Sciences
Jennifer Cable, Eilika Weber-Ban, Tim Clausen, Kylie J. Walters, Michal Sharon, Daniel J. Finley, Yangnan Gu, John Hanna, Yue Feng, Sascha Martens, Anne Simonsen, Malene Hansen, Hong Zhang, Jonathan M. Goodwin, Alessio Reggio, Chunmei Chang, Liang Ge, Brenda A. Schulman, Raymond J. Deshaies, Ivan Dikic, J. Wade Harper, Ingrid E. Wertz, Nicolas H. Thoma, Mikolaj Slabicki, Judith Frydman, Ursula Jakob, Della C. David, Eric J. Bennett, Carolyn R. Bertozzi, Richa Sardana, Vinay V. Eapen, Serena Carra
Summary: Targeted protein degradation is essential for cellular function and development. This process involves tightly regulated protein degradation pathways to eliminate misfolded and aggregated proteins, adjust protein levels during cellular differentiation, and selectively eliminate target proteins. Understanding these pathways can provide insights into disease pathology and the development of novel therapeutics.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jin Seok Shin, Si Hoon Park, Leehyeon Kim, Jiwon Heo, Hyun Kyu Song
Summary: The cellular glucose level is regulated by various cellular processes, with GID E3 ubiquitin ligase playing a role in degrading gluconeogenic enzymes. While Gid4 recognizes target substrates in normal conditions, Gid10 controls the degradation process under stress, with different substrate specificities. The structure of Gid10 in complex with Pro/N-degron provides insights into the substrate specificity within the GID E3 ligase complex.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Isha Sengupta, Payel Mondal, Amrita Sengupta, Atanu Mondal, Vipin Singh, Swagata Adhikari, Sinjini Dhang, Siddhartha Roy, Chandrima Das
Summary: During HBV infection, the upregulation of FBP1 and the reprogramming of Sp110 protein contribute to the progression of HBV-induced HCC. High expression of Sp110 can serve as a prognostic marker and a potential therapeutic target for HBV-induced HCCs.
Review
Biochemistry & Molecular Biology
Yan Zhu, Lukman O. Afolabi, Xiaochun Wan, Joong Sup Shim, Liang Chen
Summary: Neurodegenerative diseases are characterized by the degeneration of the central or peripheral nervous systems and the aggregation of misfolded proteins, leading to cellular dysfunction and brain damage. TRIM proteins play important roles in maintaining protein quality control and clearing misfolded protein aggregates associated with neurodegenerative diseases.
Article
Multidisciplinary Sciences
Francis W. Farley, Ryan R. McCully, Paul B. Maslo, Lu Yu, Mark A. Sheff, Homayoun Sadeghi, Elaine A. Elion
Summary: This study shows that Ste5 associates with heat shock protein 70 chaperone Ssa1, and Ssa1 and its ortholog Ssa2 are important for Ste5 function and efficient mating responses. Loss of Ssa1 and Ssa2 has deleterious effects on Ste5 abundance, integrity, and localization, and reduces activation of Fus3 and Kss1 MAPKs and FUS1 gene expression.
Article
Biochemical Research Methods
Yassene Mohammed, Pallab Bhowmick, Sarah A. Michaud, Albert Sickmann, Christoph H. Borchers
Summary: The laboratory mouse is a commonly used animal model in biological research, and researchers use it to study various questions such as gene effects and drug metabolism. A knowledgebase called MouseQuaPro has been developed to collect protein concentration data from over 5000 mouse tissues, providing insights into differences between tissues, strains, and sexes.
Article
Genetics & Heredity
Andreas Hentschel, Artur Czech, Ute Muenchberg, Erik Freier, Ulrike Schara-Schmidt, Albert Sickmann, Jens Reimann, Andreas Roos
Summary: Using human skin fibroblasts for proteomic studies revealed a wide variety of proteins related to genetic forms of motoneuron diseases, congenital myasthenic syndromes, neuropathies, and muscle disorders, suggesting the suitability of human skin fibroblasts to study NMD etiology. Proteomic signature of fibroblasts derived from an Allgrove patient showed dysregulation of paradigmatic proteins, which could be confirmed in a muscle biopsy and linked to neurological symptoms. Additionally, proteomic investigation of nuclear protein composition identified protein dysregulations consistent with structural perturbations observed in muscle biopsy, while fluorescence and microscopy techniques indicated altered lipid storage as part of the underlying disease etiology.
ORPHANET JOURNAL OF RARE DISEASES
(2021)
Article
Physiology
Tatjana Straka, Charlotte Schroder, Andreas Roos, Laxmikanth Kollipara, Albert Sickmann, Marion Patrick Ivey Williams, Mathias Hafner, Muzamil Majid Khan, Rudiger Rudolf
Summary: Recent studies have shown that neuromuscular junctions are co-innervated by sympathetic neurons, which are crucial for maintaining the morphology and function of neuromuscular junctions. Research using various approaches has revealed that sympathetic innervation plays a significant role in muscle and neuromuscular junction homeostasis, leading to changes such as reduced fiber diameters and enhanced acetylcholine receptor turnover. These findings suggest an upregulation of endocytic and autophagic trafficking at the whole muscle level and at the neuromuscular junction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Hematology
Laura Beckmann, Valeska Berg, Clarissa Dickhut, Clare Sun, Olaf Merkel, Johannes Bloehdorn, Sandra Robrecht, Marc Seifert, Alexandra da Palma Guerreiro, Julia Claasen, Stefan Loroch, Matteo Oliverio, Chingiz Underbayev, Lauren Vaughn, Daniel Thomalla, Malte F. Huelsemann, Eugen Tausch, Kirsten Fischer, Anna Maria Fink, Barbara Eichhorst, Albert Sickmann, Clemens M. Wendtner, Stephan Stilgenbauer, Michael Hallek, Adrian Wiestner, Rene P. Zahedi, Lukas P. Frenzel
Summary: BTK inhibitors are effective in treating CLL, with potential differential effects on non-mutated versus mutated CLL cells. Ibrutinib has a stronger impact on protein phosphorylation levels in non-mutated CLL, particularly on tyrosine phosphorylation. The protein MARCKS plays a significant role in CLL diagnosis and treatment outcomes, with low expression associated with increased leukocytosis and decreased nodal disease in CLL patients.
Review
Biochemistry & Molecular Biology
Olga Shevchuk, Antonija Jurak Begonja, Stepan Gambaryan, Matthias Totzeck, Tienush Rassaf, Tobias B. Huber, Andreas Greinacher, Thomas Renne, Albert Sickmann
Summary: This review discusses the role of proteomics in understanding the molecular basics of traditional and emerging functions of platelets, as well as the application of proteomic technologies in platelet biogenesis, signaling, and storage. Additionally, the potential of newly appeared trapped ion mobility spectrometry (TIMS) in platelet research is highlighted, along with discussions on the implementation of proteomic methods in platelet transfusion medicine, diagnostics, and prognostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Amol Fatangare, Andreas Glaessner, Bernhardt Sachs, Albert Sickmann
Summary: This article discusses the integration of innovative techniques in the lymphocyte transformation test, including cell sorting and omics techniques. These technologies have the potential to improve the sensitivity and specificity of the LTT, as well as to analyze molecular changes in immune cells induced by drugs.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Article
Biochemical Research Methods
Hilaire Yam Fung Cheung, Cristina Coman, Philipp Westhoff, Mailin Manke, Albert Sickmann, Oliver Borst, Meinrad Gawaz, Steve P. Watson, Johan W. M. Heemskerk, Robert Ahrends
Summary: This study presents a quantitative targeted ion chromatography-mass spectrometry-based workflow for measuring phosphoinositides, which improves the accuracy of quantification. The workflow was validated in various tissues and demonstrated robustness for analyzing less-abundant phosphoinositides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Multidisciplinary Sciences
Marco Heestermans, Clement Naudin, Reiner K. Mailer, Sandra Konrath, Kristin Klaetschke, Anne Jamsa, Maike Frye, Carsten Deppermann, Giordano Pula, Piotr Kuta, Manuel A. Friese, Mathias Gelderblom, Albert Sickmann, Roger J. S. Preston, Jerzy-Roch Nofer, Stefan Rose-John, Lynn M. Butler, Ophira Salomon, Evi X. Stavrou, Thomas Renne
Summary: The study reveals the critical role of the Gln317-Ser339 region in FXII protein in blood coagulation and thrombus formation, with the potential for antibody-induced activation of FXII to be used in sensitive monitoring of coagulation factors.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Jingnan Huang, Frauke Swieringa, Fiorella A. Solari, Isabella Provenzale, Luigi Grassi, Ilaria De Simone, Constance C. F. M. J. Baaten, Rachel Cavill, Albert Sickmann, Mattia Frontini, Johan W. M. Heemskerk
Summary: Novel analysis of platelet and megakaryocyte transcriptome revealed high similarity between their transcriptomes, with redistribution of mRNAs upon platelet shedding from megakaryocytes. A prediction model based on protein function, transcript level and (peri)nuclear localization estimated the achievable proteome of about 10 k proteins, with identification of additional proteins related to thrombosis and platelet disorders. This genome-wide platelet transcriptome provides a valuable resource for studying the roles of unknown platelet proteins in health and disease.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Constanze Schanbacher, Michael Bieber, Yvonne Reinders, Deya Cherpokova, Christina Teichert, Bernhard Nieswandt, Albert Sickmann, Christoph Kleinschnitz, Friederike Langhauser, Kristina Lorenz
Summary: The activation or inhibition of the ERK1/2 signaling cascade has a significant impact on the outcome of ischemic stroke. This study found that overexpression of wild-type ERK2 worsened the infarct volume and neurological deficits, as well as increased blood-brain barrier leakage, inflammation, and apoptotic neurons. In contrast, inhibition of the Raf/MEK/ERK1/2 cascade provided protection against ischemic damage, primarily through anti-inflammatory signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Andrea Gangfuss, Artur Czech, Andreas Hentschel, Ute Munchberg, Rita Horvath, Ana Topf, Emily O'Heir, Hanns Lochmuller, Florian Stehling, Cordula Kiewert, Albert Sickmann, Alma Kuechler, Frank J. Kaiser, Heike Kolbel, Jon Christiansen, Ulrike Schara-Schmidt, Andreas Roos
Summary: Recessive variants in WASHC4 are associated with intellectual disability, poor language skills, and other complications. While studies in a zebrafish model suggest that WASHC4 knockdown may affect skeletal muscles, the involvement of skeletal muscles in patients has not been reported, indicating a need for further research.
JOURNAL OF PATHOLOGY
(2022)
Article
Genetics & Heredity
Annabelle Arlt, Nicolai Kohlschmidt, Andreas Hentschel, Enrika Bartels, Claudia Gross, Ana Toepf, Pinar Edem, Nora Szabo, Albert Sickmann, Nancy Meyer, Ulrike Schara-Schmidt, Jarred Lau, Hanns Lochmueller, Rita Horvath, Yavuz Oktay, Andreas Roos, Semra Hiz
Summary: Goltz syndrome is an X-linked disorder characterized by skin pigmentation, ocular and skeletal malformations, and male in utero lethality. Neurological deficits in GS are rare, with less severe phenotypes potentially caused by non-mosaic survival in males or less pathogenic mutations. Additional molecular genetics and functional work-up is needed to understand the rare variants.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Julia Hesse, Mona K. Rosse, Bodo Steckel, Bernhard Blank-Landeshammer, Svenja Idel, Yvonne Reinders, Albert Sickmann, Norbert Straeter, Juergen Schrader
Summary: CD73-derived adenosine plays a major role in damage-induced tissue responses by inhibiting inflammation. This study suggests that human CD73 enzymatic activity is regulated by mono-ADP-ribosylation, which can profoundly modulate its adenosine-generating activity.
PURINERGIC SIGNALLING
(2022)
Article
Endocrinology & Metabolism
Miriam Hoene, Lisa Kappler, Laxmikanth Kollipara, Chunxiu Hu, Martin Irmler, Daniel Bleher, Christoph Hoffmann, Johannes Beckers, Martin Hrabe de Angelis, Hans-Ulrich Haring, Andreas L. Birkenfeld, Andreas Peter, Albert Sickmann, Guowang Xu, Rainer Lehmann, Cora Weigert
Summary: The study investigated how exercise affects the early compensatory response of liver mitochondria to high-calorie intake to prevent fatty liver disease. Training modified hepatic metabolism adaptation by improving glucose metabolism, lowering liver fat, and enhancing mitochondrial oxidative capacity. In skeletal muscle, a combination of high-energy diet and training significantly increased oxidative capacity.
MOLECULAR METABOLISM
(2021)
Article
Cell Biology
Adela Della Marina, Annabelle Arlt, Ulrike Schara-Schmidt, Christel Depienne, Andrea Gangfuss, Heike Koelbel, Albert Sickmann, Erik Freier, Nicolai Kohlschmidt, Andreas Hentschel, Joachim Weis, Artur Czech, Anika Grueneboom, Andreas Roos
Summary: A study identified compound heterozygous missense and nonsense variants in the SLC18A3 gene associated with congenital myasthenic syndrome, with the nonsense variant potentially causing impaired motor and cognitive development and limited treatment efficacy. Muscle biopsy showed reduced fibre size and lipid droplet accumulation, suggesting the impact of SLC18A3 variants on muscle integrity and lipid homeostasis. The importance of proper VAChT-mediated synthesis and recycling of ACh for muscle cells' lipid balance is highlighted by these findings.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)