Review
Biochemistry & Molecular Biology
Iason Psilopatis, Eleni Souferi-Chronopoulou, Kleio Vrettou, Constantinos Troungos, Stamatios Theocharis
Summary: EPHs/ephrins appear to be promising treatment targets in the future for breast cancer, with EPHA2 being the most studied target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Iason Psilopatis, Ioannis Karniadakis, Konstantinos Stylianos Danos, Kleio Vrettou, Kleita Michaelidou, Konstantinos Mavridis, Sofia Agelaki, Stamatios Theocharis
Summary: Lung cancer is the leading cause of cancer death in the United States. Erythropoietin-producing hepatocellular receptors (EPHs) and their ligands ephrins play a defining role in the development and progression of lung cancer. Targeting EPHs/ephrins may provide effective treatment options for precision cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yan Xu, Dorothea Robev, Nayanendu Saha, Bingcheng Wang, Matthew B. Dalva, Kai Xu, Juha P. Himanen, Dimitar B. Nikolov
Summary: Eph receptor tyrosine kinases and ephrin ligands play crucial roles in cell communication, with dysfunctional signaling leading to disease. The formation of extensive signaling assemblies regulates neuronal migration and axon pathfinding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Endocrinology & Metabolism
Michael C. Lawrence
Summary: The article describes the three-dimensional structure of insulin and its receptor, as well as their interactions, highlighting research gaps in structural understanding. A near-complete picture has been obtained of hormone receptor interactions, providing new insights and requiring revision of the existing hormone receptor engagement models.
MOLECULAR METABOLISM
(2021)
Review
Biochemistry & Molecular Biology
Sakshi Arora, Andrew M. Scott, Peter W. Janes
Summary: Eph receptor tyrosine kinases play critical roles in development and tissue formation, and are regulated by membrane-bound ligands ephrins. They have low expression in adults, but are often overexpressed in cancers, where they are associated with tumour development, vasculature, and invasion. Understanding the opposing roles of Ephs in different cancers is crucial for developing targeted therapies.
Review
Reproductive Biology
Enoch Appiah Adu-Gyamfi, Armin Czika, Tai-Hang Liu, Philip Narteh Gorleku, Linda Ahenkorah Fondjo, Francis Tanam Djankpa, Yu-Bin Ding, Ying-Xiong Wang
Summary: Ephrins and Eph receptors play important roles in the female reproductive system, regulating various processes and being associated with obstetric and gynecologic disorders.
BIOLOGY OF REPRODUCTION
(2021)
Article
Chemistry, Medicinal
Maricel Gomez-Soler, Erika J. Olson, Elena Rubio de la Torre, Chunxia Zhao, Ilaria Lamberto, Dillon T. Flood, Waleed Danho, Bernhard C. Lechtenberg, Stefan J. Riedl, Elena B. Pasquale, Philip E. Dawson
Summary: The EphA4 receptor tyrosine kinase is involved in several diseases and inhibiting its activity could have therapeutic potential. Researchers have identified a peptide antagonist that specifically inhibits the binding of ephrin ligands to EphA4. They have also modified the peptide to improve its stability and activity. These derivatives could be useful for studying EphA4 in vivo and for testing EphA4 inhibition in animal models.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Amita R. Sahoo, Matthias Buck
Summary: This review focuses on the interaction between Eph receptors and ephrin ligands in regulating various processes from embryonic development to tissue homeostasis. It also highlights the importance of the transmembrane domain of Eph receptors in signal transduction and introduces techniques such as NMR and molecular modeling used to study them. Additionally, the use of transmembrane peptides to manipulate Eph receptor signaling is discussed, along with future directions for research in this field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Michal Mayer, Yulia Matiuhin, Mickal Nawatha, Orly Tabachnikov, Inbar Fish, Nili Schutz, Hay Dvir, Meytal Landau
Summary: The bacterial tyrosine kinase BceF from Burkholderia cepacia is structurally characterized in this study and found to have unique variations compared to other BY-kinases, offering a potential for development of specific inhibitors targeting Burkholderia cepacia virulence.
Review
Cell Biology
David G. Wilkinson
Summary: Cell segregation to form sharp boundaries is crucial for stabilizing tissue organization and is driven by differential adhesion mediated by cadherins and Eph receptor/ephrin signaling regulating cell adhesion, cortical tension, and repulsion. These mechanisms interplay with cadherins antagonizing homotypic tension within cell populations and Eph receptors mainly driving segregation through increased heterotypic tension or repulsion. The differential tension between homotypic and heterotypic tensions ultimately leads to cell segregation and border sharpening.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Siu Wai Wong, Lucy Vivash, Ramesh Mudududdla, Nghi Nguyen, Stefan J. Hermans, David M. Shackleford, Judith Field, Lian Xue, Andrea Aprico, Nancy C. Hancock, Mohammad Haskali, Michael A. Stashko, Stephen Frye, Xiaodong Wang, Michele D. Binder, Uwe Ackermann, Michael W. Parker, Trevor J. Kilpatrick, Jonathan B. Baell
Summary: A new [F-18] ligand with high affinity and selectivity for MERTK as a potential PET radiotracer was successfully developed in this study. The compound showed significant specific binding to MERTK, especially in specific regions of the brain, indicating potential clinical utility.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Gavin W. Collie, Iacovos N. Michaelides, Kevin Embrey, Christopher J. Stubbs, Ulf Borjesson, Ian L. Dale, Arjan Snijder, Louise Barlind, Kun Song, Puneet Khurana, Christopher Phillips, R. Ian Storer
Summary: A fragment screen targeting the c-MET kinase led to the discovery of inhibitors binding to a rare conformation of the protein, with one inhibitor (7) showing nanomolar biochemical activity against c-MET. These findings enhance our understanding of the folded P-loop conformation of c-MET and provide a basis for further exploration of this potentially therapeutically exploitable state.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Multidisciplinary Sciences
Yihu Xie, Christopher L. Lord, Bradley P. Clarke, Austin L. Ivey, Pate S. Hill, W. Hayes McDonald, Susan R. Wente, Yi Ren
Summary: CTDK-1 is the primary RNA Pol II CTD Ser2 kinase complex in budding yeast, consisting of a CDK Ctk1, a cyclin Ctk2, and a unique subunit Ctk3 which activates Ctk1 by stabilizing the T-loop. Additionally, Ctk3 contributes to the assembly of CTDK-1 through interactions with both Ctk1 and Ctk2, and physically interacts with Gbp2, a serine/arginine-like protein. These findings reveal a regulatory mechanism of CDK complexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Priya Jarwar, Shakeel Ahmed Sheikh, Yar Muhammad Waryah, Ikram Uddin Ujjan, Saima Riazuddin, Ali Muhammad Waryah, Zubair M. Ahmed
Summary: Hereditary congenital cataract (HCC) is clinically and genetically heterogeneous, with cataracts segregating in a recessive manner in three families with variability related to age of onset. Exome sequencing identified a novel EPHA2 variant in LUCC03 and a known variant in the other families. Computational modeling predicted that the variants cause misfolding of EPHA2. This study provides insights into the molecular and phenotypic landscape of EPHA2-related HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Sahibpreet Kaur, Anjana Bali, Nirmal Singh, Amteshwar Singh Jaggi
Summary: Ephrins and Eph receptors play a crucial role in nervous system development, including axon guidance and cell migration. Upregulation of ephrin B1/EphB1 and ephrin B2/EphB2 has been observed in neuropathic pain of different etiology. Activation of the ephrin B/EphB system in the dorsal root ganglion and dorsal horn of the spinal cord may be essential in initiating and maintaining neuropathic pain. Inhibitors of EphB receptors could potentially be used to manage pain symptoms. Ephrin B/EphB-mediated synaptic plasticity involves phosphorylation and activation of NMDA receptors and other molecular mechanisms, including activation of inflammatory cytokines, caspase-3, calpain-1, PI3K, PKA, and CREB. This review discusses the role and molecular mechanisms involved in ephrin B/EphB-mediated neuropathic pain.
FUNDAMENTAL & CLINICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)