期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 393, 期 4, 页码 587-591出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.02.019
关键词
Glycine receptor; Chloride channel; Single-channel conductance; Hippocampus; Mossy fiber; Development
资金
- Hertie Foundation
- Max-Planck Society
- Deutsche Forschungsgemeinschaft
- Fonds der Chemischen Industrie
Presynaptic glycine receptors (GlyRs) have been implicated in the regulation of glutamatergic synaptic transmission. Here, we characterized presynaptic GlyR-mediated currents by patch-clamp recording from mossy fiber boutons (MFBs) in rat hippocampal slices. In MFBs, focal puff-application of glycine-evoked chloride currents that were blocked by the GlyR antagonist strychnine. Their amplitudes declined substantially during postnatal development, from a mean conductance per MFB of similar to 600 pS in young to similar to 130 pS in adult animals. Single-channel analysis revealed multiple conductance states between 20 and similar to 120 pS, consistent with expression of both homo- and hetero-oligomeric GlyRs. Accordingly, estimated GlyRs densities varied between 8-17 per young, and 1-3 per adult, MFB. Our results demonstrate that functional presynaptic GlyRs are present on hippocampal mossy fiber terminals and suggest a role of these receptors in the regulation of glutamate release during the development of the mossy fiber - CA3 synapse. (C) 2010 Elsevier Inc. All rights reserved.
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