期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 401, 期 1, 页码 143-148出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.09.030
关键词
AHNAK1; AHNAK2; Costamer; Fiber stiffness; Membrane repair; Skeletal muscle
资金
- Deutsche Forschungsgemeinschaft Grant Clinical Research Unit [192]
The AHNAK scaffold PDZ-protein family is implicated in various cellular processes including membrane repair; however, AHNAK function and subcellular localization in skeletal muscle are unclear. We used specific AHNAK1 and AHNAK2 antibodies to analyzed the detailed localization of both proteins in mouse skeletal muscle. Co-localization of AHNAK1 and AHNAK2 with vinculin clearly demonstrates that both proteins are components of the costameric network. In contrast, no AHNAK expression was detected in the T-tubule system. A laser wounding assay with AHNAK1-deficient fibers suggests that AHNAK1 is not involved in membrane repair. Using atomic force microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1(-/-) fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle. (C) 2010 Elsevier Inc. All rights reserved.
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