Article
Biology
Eugene Serebryany, Sourav Chowdhury, Christopher N. Woods, David C. Thorn, Nicki E. Watson, Arthur A. McClelland, Rachel E. Klevit, Eugene Shakhnovich
Summary: Cataract, a protein aggregation disorder, is a common cause of vision loss worldwide. Researchers have discovered that myo-inositol, an abundant lens metabolite, can suppress the aggregation of lens crystallins, suggesting it as a potential strategy to prevent or delay age-onset cataracts.
Article
Biochemistry & Molecular Biology
Susanne Weininger, Malte Neudorf, Stefan Groeger, Eric Plato, Robert Broneske, Kay Saalwaechter, Ulrich Weininger, Jochen Balbach
Summary: Crystallin proteins in the human eye lenses play a role in maintaining transparency, light refraction, and UV light protection. Imbalance in the interaction between alpha-, beta-, and gamma-crystallin can lead to cataracts. The research looks into the effects of UV-B radiation on gamma D-crystallin, specifically observing changes in the N-terminal domain. It is found that some photoprotective properties remain in extracts from cataract patients. Additionally, a genetic mutation in the eye lens core of infants with cataracts increases sensitivity to UV-B irradiation.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Multidisciplinary Sciences
Roy Joseph, Michael L. L. Robinson, Laura Lambert, Om P. P. Srivastava
Summary: The beta A3/A1-crystallin protein is important in maintaining lens transparency and the loss of its function leads to congenital cataract in mice. The activation of calpain 3 results in degradation of beta-tubulin and other lens proteins, causing protein accumulation and disruption of cellular microtubular structure, which contributes to the development of cataract in beta A3/A1cKO mice.
Article
Zoology
Zhe-Kun Jia, Chen-Xi Fu, Ai-Ling Wang, Ke Yao, Xiang-Jun Chen
Summary: This study revealed that the up-regulated genes in CRYAA-Y118D mutant mice were implicated in the endoplasmic reticulum stress (ERS) - unfolded protein response (UPR) pathway, leading to proteotoxicity and cell death. The CRYAA-Y118D mutant mice showed severe cataract-related phenotypes and aberrant arrangements of crystallin fibers, providing insights into the pathogenic mechanisms underlying cataract formation.
ZOOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jingjie Xu, Huaxia Wang, Ailing Wang, Jia Xu, Chenxi Fu, Zhekun Jia, Ke Yao, Xiangjun Chen
Summary: Congenital cataract is a major cause of blindness in children globally. A novel beta B2 mutation W151R was found to be prone to aggregation due to low solubility and poor structural stability, but the negative effect of the mutation was reversed by administration of lanosterol.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Cell Biology
Yuan Xiao, Jia-Wen Xiang, Qian Gao, Yue-Yue Bai, Zhao-Xia Huang, Xiao-Hui Hu, Ling Wang, David Wan-Cheng Li
Summary: Research has shown that Mab21L1 promotes survival of lens epithelial cells by regulating the expression of αB-crystallin and suppressing the ATR/CHK1/p53 pathway. This finding contributes to a better understanding of the functional mechanism of Mab21L1.
Article
Biochemistry & Molecular Biology
Ying Zhang, Ling Ren, Wei Wu, Jian Liu, Qing Tian, Ke Yao, Yibo Yu, Lidan Hu, Xiangjun Chen
Summary: Congenital cataract is a major cause of childhood blindness worldwide. This study investigated the molecular mechanism of the ss B1-Q70P mutation in congenital cataract at the molecular, protein, and cellular levels. The study found that ss B1-Q70P significantly changed the structures of ss B1-crystallin and exhibited lower solubility, leading to cellular aggregation and impaired viability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Amber D. Rolland, Takumi Takata, Micah T. Donor, Kirsten J. Lampi, James S. Prell
Summary: This study investigates the interactions and oligomerization kinetics of β-crystallin in the lens using native ion mobility-mass spectrometry, revealing compact ring-like topologies of the observed oligomers.
Article
Biochemistry & Molecular Biology
Jing Wu, Silong Chen, Jingjie Xu, Wanyue Xu, Sifan Zheng, Qing Tian, Chenqi Luo, Xiangjun Chen, Xingchao Shentu
Summary: Congenital cataracts account for a significant percentage of childhood blindness worldwide, particularly in developing countries. A G149V point missense mutation in beta B2-crystallin was found to be associated with congenital cataracts in a Chinese family. This study investigated the structural and biophysical effects of the G149V mutation, revealing changes in the protein's structure, stability, and sensitivity to environmental stresses. These findings provide insights into the pathogenesis of the G149V mutant in congenital cataracts.
Article
Cell Biology
Xiang-Jia Zhu, Ke-Ke Zhang, Wen-Wen He, Jiao Qi, Yi Lu
Summary: Compared to age-related cataracts, diabetic cataracts exhibit a distinct pattern of protein racemization, which may influence the progression of the disease over time.
Article
Cell Biology
Minglei Zhao, Tingfang Mei, Bizhi Shang, Bin Zou, Qing Lian, Wenchang Xu, Keling Wu, Yuhua Lai, Chujun Liu, Lai Wei, Jie Zhu, Kang Zhang, Yizhi Liu, Ling Zhao
Summary: The study demonstrates that a mouse model carrying Lss G589S mutation can recapitulate human congenital cataract and indicates the critical role of LSS in lens development. The mutation leads to opacity in eye lens formation, disrupted lens differentiation, and downregulation of cholesterol synthesis signaling pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ningqin Lin, Ying Zhang, Xiaohui Song, Jingjie Xu, Chenqi Luo, Qing Tian, Ke Yao, Wei Wu, Xiangjun Chen, Lidan Hu
Summary: In this study, the pathogenic mechanisms of two mutations in the gamma D-crystallin were investigated, revealing structural disruption and aggregation formation. Furthermore, it was found that the aggregation of the mutants could be reversed by lanosterol, suggesting a potential therapeutic strategy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Giovanni Palomino-Vizcaino, Nils Schuth, Jose A. Dominguez-Calva, Oscar Rodriguez-Meza, Eduardo Martinez-Jurado, Eugene Serebryany, Jonathan A. King, Thomas Kroll, Miguel Costas, Liliana Quintanar
Summary: Cataracts are caused by the aggregation of high-molecular-weight proteins in the human eye lens, which scatter light and cause lens opacity. Metal ions play an important role in the development of cataract disease, as human lens gamma-crystallins are susceptible to metal-induced aggregation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Cell Biology
Samuel G. Novo, Adam P. Faranda, Mahbubul H. Shihan, Yan Wang, Ananya Garg, Melinda K. Duncan
Summary: Cataract surgery leaves behind lens epithelial cells (LECs) that trigger a wound healing response, and immediate early transcription factors (IETFs) are involved in regulating this response.
Article
Biochemistry & Molecular Biology
Jing Wu, Wanyue Xu, Wei Wu, Jingjie Xu, Sifan Zheng, Xingchao Shentu, Xiangjun Chen
Summary: This study investigated the molecular mechanism of the R48C mutation of gamma A-crystallin in a Mexican-Mestizo descent family causing congenital cataracts. The R48C mutation did not affect the secondary and tertiary structure of the protein, but it did disrupt its oxidative stability, leading to increased aggregation and precipitation under oxidative conditions, potentially contributing to the pathogenesis of congenital cataracts related to this mutation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Leah N. Makley, Kathryn A. McMenimen, Brian T. DeVree, Joshua W. Goldman, Brittney N. McGlasson, Ponni Rajagopal, Bryan M. Dunyak, Thomas J. McQuade, Andrea D. Thompson, Roger Sunahara, Rachel E. Klevit, Usha P. Andley, Jason E. Gestwicki
Article
Biochemistry & Molecular Biology
Usha P. Ley, Joshua W. Goldman
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2016)
Article
Biochemistry & Molecular Biology
Usha P. Andley, James P. Malone, Paul D. Hamilton, Nathan Ravi, R. Reid Townsend
Article
Chemistry, Medicinal
Baozhong Zhao, Yu-Ying He, Colin F. Chignell, Jun-Jie Yin, Usha Andley, Joan E. Roberts
CHEMICAL RESEARCH IN TOXICOLOGY
(2009)
Article
Ophthalmology
Usha P. Andley, Matthew A. Reilly
EXPERIMENTAL EYE RESEARCH
(2010)
Article
Ophthalmology
Jonathan A. Wignes, Joshua W. Goldman, Conrad C. Weihl, Matthew G. Bartley, Usha P. Andley
EXPERIMENTAL EYE RESEARCH
(2013)
Correction
Biochemistry & Molecular Biology
Jing-hua Xi, Fang Bai, Julia Gross, R. Reid Townsend, A. Sue Menko, Usha P. Andley
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Ophthalmology
Ashish Sharma, Ashkan Pirouzmanesh, Jayaprakash Patil, M. F. Estrago-Franco, Leandro Cabral Zacharias, Aslan Pirouzmanesh, Usha P. Andley, M. Cristina Kenney, Baruch D. Kuppermann
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
(2011)
Article
Biochemistry & Molecular Biology
Baozhong Zhao, Colin F. Chignell, Mustapha Rammal, Frank Smith, Mary G. Hamilton, Usha P. Andley, Joan E. Roberts
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2010)
Article
Biochemistry & Molecular Biology
Marilyn Ehrenshaft, Baozhong Zhao, Usha P. Andley, Ronald P. Mason, Joan E. Roberts
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2011)
Article
Multidisciplinary Sciences
Usha P. Andley, Paul D. Hamilton, Nathan Ravi, Conrad C. Weihl
Article
Multidisciplinary Sciences
Usha P. Andley, James P. Malone, R. Reid Townsend
Article
Ophthalmology
Usha P. Andley, James P. Malone, R. Reid Townsend
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2011)
Article
Biochemistry & Molecular Biology
Matthew A. Reilly, Usha P. Andley
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)