期刊
ANALYST
卷 140, 期 19, 页码 6671-6675出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an00726g
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资金
- National Research Foundation of Korea (NRF) - Korea Government (MEST) [2013R1A1A2021531]
We present a novel reflectance-based colorimetric aptasensor using gold nanoparticles for the detection of oxytetracycline for the first time. It was found that the reflectance-based measurement at two wavelengths (650 and 520 nm) can generate more stable and sensitive signals than absorbance-based sensors to determine the aggregation of AuNPs, even at high AuNP concentrations. One of the most common antibacterial agents, oxytetracycline (OTC), was detected at concentrations as low as 1 nM in both buffer solution and tap water, which was 25-fold more sensitive, compared to the previous absorbance-based colorimetric aptasensors. This reflectance-based colorimetric aptasensor using gold nanoparticles is considered to be a better platform for portable sensing of small molecules using aptamers.
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