Article
Cardiac & Cardiovascular Systems
Houwei Li, Xue Liu, Na Sun, Tianshuo Wang, Jia Zhu, Shuang Yang, Xia Song, Ruishuai Wang, Xinhui Wang, Yixiu Zhao, Yan Zhang
Summary: This study identified differentially expressed circRNAs in atherosclerotic aortic vessels, with notable up-regulation of Mmu_circRNA_36781 and 37699. These circRNAs have potential regulatory effects on atherosclerosis and vascular endothelial injury by targeting the miR-30d-3p-TP53RK and miR-140-3p-MKK6 axis, as well as their downstream signaling pathways.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Maria Vincenza Chiantore, Marco Iuliano, Roberta Maria Mongiovi, Fabiola Luzi, Giorgio Mangino, Lorenzo Grimaldi, Luisa Accardi, Gianna Fiorucci, Giovanna Romeo, Paola Di Bonito
Summary: Actinic keratosis (AK) is a precursor to cutaneous squamous cell carcinoma (cSCC) and is highly prevalent in the elderly population, particularly among those with sun-exposed skin. This study identified 43 dysregulated microRNAs (miRNAs) in AK samples and validated the expression of several key miRNAs. Pathway analysis revealed that these miRNAs targeted pathways involved in tumorigenesis, highlighting the importance of miRNA regulation in keratinocyte cancer.
Article
Biochemical Research Methods
Bhaswati Sarcar, Bin Fang, Victoria Izumi, Yury O. Nunez Lopez, Alexandra Tassielli, Richard Pratley, Daniel Jeong, Jennifer B. Permuth, John M. Koomen, Jason B. Fleming, Paul A. Stewart
Summary: Small extracellular vesicles (sEVs) released from pancreatic stellate cells (HPSCs) play a role in pancreatic ductal adenocarcinoma (PDAC) progression. This study compares sEVs from normal pancreatic stellate cells (HPaStec) to sEVs from PDAC-associated stellate cells (HPSCs), and identifies differential protein expression between the two. Although sEVs do not directly affect cancer cells, they are efficiently taken up by both normal and cancer epithelial cells. The presence of certain protein markers, such as CSE1L, in HPSC sEVs suggests their significance in poor prognosis of pancreatic cancer patients.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Endocrinology & Metabolism
Zhihong Wang, Ramkumar Mohan, Xinqian Chen, Katy Matson, Jackson Waugh, Yiping Mao, Shungang Zhang, Wanzhen Li, Xiaohu Tang, Leslie S. Satin, Xiaoqing Tang
Summary: MiR-483 plays a critical role in protecting beta-cell function, with its absence leading to hyperglycemia, impaired insulin secretion, and initiation of beta-cell dedifferentiation in the development of type 2 diabetes.
Article
Clinical Neurology
Yanyan Jiang, Jing Chen, Yunchuang Sun, Fan Li, Luhua Wei, Wei Sun, Jianwen Deng, Yun Yuan, Zhaoxia Wang
Summary: The combination of salivary miR-29a-3p and miR-29c-3p shows potential as a diagnostic biomarker for idiopathic PD. The expression level of miR-29a-3p in PD patients was significantly lower than ET patients, but higher than MSA patients.
FRONTIERS IN NEUROLOGY
(2021)
Article
Genetics & Heredity
Feng Xu, Yifan Wang, Yunchao Ling, Chenfen Zhou, Haizhou Wang, Andrew E. Teschendorff, Yi Zhao, Haitao Zhao, Yungang He, Guoqing Zhang, Zhen Yang
Summary: MicroRNAs play important roles in gene expression regulation, and their dysregulation is widely reported in pathological cellular states. The dbDEMC database was constructed to store and query differentially expressed miRNAs (DEMs) in human cancers. The updated version, dbDEMC 3.0, contains more data entries and includes data from mice and rats. It provides a valuable resource for studying the molecular functions and regulatory mechanisms of DEMs in human cancers.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Young-Gon Kim, Jisook Park, Eun Young Park, Sang-Mi Kim, Soo-Youn Lee
Summary: This study investigated the response of normal pancreatic islet cells to pancreatic cancer-derived exosomes in order to identify potential biomarkers for pancreatic cancer-induced diabetes mellitus (PC-DM) and pancreatic cancer itself. Changes in microRNA expression were evaluated, and 24 candidate miRNA markers were proposed. These markers showed relevance to diabetes mellitus and/or insulin resistance, suggesting a potential association with PC-DM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Jin-Sook Kwon, Eric W. Barr, J. Kurt Chuprun, Walter J. Koch
Summary: Stimulation of alpha- or beta-adrenergic receptors in mice led to changes in the content of circulating blood EVs, with differential expression patterns of miRs. Pathway analysis revealed common and distinct pathways affected by PE and ISO treatment, indicating regulation of specific pathways by alpha- or beta-AR agonists. This study suggests that chronic sympathetic nervous system activation in heart failure may alter the content of EVs secreted from the heart and other organs, potentially impacting pathological remodeling.
Article
Andrology
Jing Ma, Ruiyu Han, Bo Sun, Jiajie Lin, Peipei Deng, Shusong Wang, Shaoguang Sun
Summary: This study identified 39 differentially expressed miRNAs in testicular tissues of rats with hyperuricaemia-induced male infertility. These miRNAs could shed light on the mechanism of hyperuricaemia-induced male infertility.
Article
Genetics & Heredity
Gaoxiao Xu, Yamei Hu, Dongling Yu, Xingfa Chen, Xiao Li, Saixing Duan, Ning Zhang, Gaoyu Xu, Jianhong Hu, Gongshe Yang, Shiduo Sun, Yong Liu
Summary: This study investigated the expression profiles of microRNAs (miRNAs) in the ovaries of sows with larger and smaller than average litter sizes, and found that miR-183 plays an important role in promoting granulosa cell proliferation and regulating the synthesis of estradiol and progesterone in pig ovaries.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Huixia Ren, Yanjun Li, Chengsheng Han, Yi Yu, Bowen Shi, Xiaohong Peng, Shufang Wu, Xiaojing Yang, Sneppen Kim, Liangyi Chen, Chao Tang, Tianming Zhang
Summary: The Ca2+ modulated pulsatile glucagon and insulin secretions by pancreatic alpha and beta cells play a crucial role in glucose homeostasis. However, the coordination between alpha and beta cells in producing different Ca2+ oscillation patterns is still unclear. This study used a microfluidic device and transgenic mice to record Ca2+ signals from islet alpha and beta cells and observed heterogeneous Ca2+ oscillation patterns intrinsic to each islet. The results showed that after glucose stimulation, the oscillations of alpha and beta cells were globally phase-locked, with a fixed time delay in the activation of alpha cells compared to beta cells and a tunable period in the activation of beta cells. The number of islet alpha cells was also found to be correlated with oscillation frequency. A mathematical model incorporating paracrine interactions was built and quantitatively agreed with the experimental data, highlighting the importance of cell-cell interaction in generating stable but tunable islet oscillation patterns.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Hao-Qi Wang, Wen-Hua Wang, Cheng-Zhen Chen, Hai-Xiang Guo, Hao Jiang, Bao Yuan, Jia-Bao Zhang
Summary: Gonadotropin-releasing hormone (GnRH) and follicle-stimulating hormone (FSH) play crucial roles in regulating mammalian reproductive activities. The study discovered the potential mechanism of miR-488 in GnRH-mediated regulation of FSH synthesis, providing additional evidence for the significance of miRNAs in animal reproduction regulation.
Article
Parasitology
Chunling Zhu, Yuting Jiang, Qianghui Zhang, Jian Gao, Chaojie Li, Chunxiao Li, Yande Dong, Dan Xing, Hengduan Zhang, Teng Zhao, Xiaoxia Guo, Tongyan Zhao
Summary: The study found that there was no significant difference in vector competence to ZIKV between the JG and MD strains of Ae. aegypti, but the JG strain showed an earlier presence of ZIKV RNA in saliva, indicating a potentially higher transmission ability. Additionally, significant differences in miRNA expression profiles between ZIKV-infected and non-infected Ae. aegypti were observed in both midgut and salivary gland tissues of the two strains.
PARASITES & VECTORS
(2021)
Article
Genetics & Heredity
Roya Ramezankhani, Afshin Abdi Ghavidel, Saadyeh Rashidi, Mahbubeh Rojhannezhad, Hamid Reza Abolkheir, Malihe Mirhosseini, Sara Taleahmad, Massoud Vosough
Summary: Pancreatic cancer is a leading cause of cancer mortality worldwide, with higher incidence and mortality rates in male patients. Efforts to improve therapeutic outcomes have been limited by low efficiency and drug resistance. This study identifies genetic factors that may link gender to pancreatic cancer progression and survival rates, highlighting the importance of androgenic effectors and axon guidance in tumorigenesis.
FRONTIERS IN GENETICS
(2022)
Article
Obstetrics & Gynecology
Ching Hung Chen, Farn Lu, Wen Jui Yang, Pok Eric Yang, Wei Ming Chen, Shih Ting Kang, Yu Shan Huang, Yi Chi Kao, Chi Ting Feng, Po Chang Chang, Tiffany Wang, Chi An Hsieh, Yu Chun Lin, Jack Yu Jen Huang, Lily Hui-Ching Wang
Summary: The study identified predictor microRNAs in patients with repeated implantation failure (RIF) by analyzing miRNA profiles from the endometrium of patients undergoing in vitro fertilization (IVF). A signature set of miRNAs associated with the risk of RIF was identified, and a 3-miRNA signature was able to predict patients with RIF with an accuracy >90% in the validation cohort. The findings suggest that specific endometrial miRNAs can be used as diagnostic biomarkers to predict RIF and potentially increase the success rate of implantation practice.
FERTILITY AND STERILITY
(2021)
Article
Gastroenterology & Hepatology
Hui Joyce Li, Subir K. Ray, Alper Kucukural, Gerard Gradwohl, Andrew B. Leiter
Summary: This study revealed that the fate of Neurog3(+) enteroendocrine progenitors is dependent on Neurog3 gene dosage, with high dosage enforcing commitment to the enteroendocrine lineage and constraining their potential to differentiate into goblet cells. The findings suggest a crucial role of Neurog3 gene dosage in controlling cell fate decisions in the intestine.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Endocrinology & Metabolism
Zhihong Wang, Ramkumar Mohan, Xinqian Chen, Katy Matson, Jackson Waugh, Yiping Mao, Shungang Zhang, Wanzhen Li, Xiaohu Tang, Leslie S. Satin, Xiaoqing Tang
Summary: MiR-483 plays a critical role in protecting beta-cell function, with its absence leading to hyperglycemia, impaired insulin secretion, and initiation of beta-cell dedifferentiation in the development of type 2 diabetes.
Article
Endocrinology & Metabolism
Ramkumar Mohan, Seokwon Jo, Amber Lockridge, Deborah A. Ferrington, Kevin Murray, Arthur Eschenlauer, Ernesto Bernal-Mizrachi, Yoshio Fujitani, Emilyn U. Alejandro
Summary: This study identified the role of OGT in regulating beta-cell mitochondrial function, showing that OGT deletion can lead to mitochondrial dysfunction. Furthermore, overexpression of Pdx1 was found to improve mitochondrial function in OGT-deficient cells.
Article
Endocrinology & Metabolism
Kaare V. Grunddal, Sarah Tonack, Kristoffer L. Egerod, Jonathan James Thompson, Natalia Petersen, Maja S. Engelstoft, Constance Vagne, Celine Keinse, Gerard Gradwohl, Stefan Offermanns, Thue W. Schwartz
Summary: The study identified the expression of GPR64 in various organs throughout the body, particularly in olfactory microvillous cells and GI tuft cells. In the GI tract, GPR64 was found to be selectively expressed in mature immunoregulatory tuft cells.
MOLECULAR METABOLISM
(2021)
Article
Medicine, Research & Experimental
Brian Akhaphong, Daniel C. Baumann, Megan Beetch, Amber D. Lockridge, Seokwon Jo, Alicia Wong, Tate Zemanovic, Ramkumar Mohan, Danica L. Fondevilla, Michelle Sia, Maria Ruth B. Pineda-Cortel, Emilyn U. Alejandro
Summary: Placental mTOR signaling plays a key role in fetal birth weight and metabolic health of offspring, and loss of placental mTOR function leads to reduced birth weight and metabolic dysfunction in newborns. Enhancing placental mTORC1 signaling can protect offspring from diet-induced obesity.
Article
Endocrinology & Metabolism
Valerie Schreiber, Reuben Mercier, Sara Jimenez, Tao Ye, Emmanuel Garcia-Sanchez, Annabelle Klein, Aline Meunier, Sabitri Ghimire, Catherine Birck, Bernard Jost, Kristian Honnens de Lichtenberg, Christian Honore, Palle Serup, Gerard Gradwohl
Summary: The study mapped the genome occupancy of the NEUROG3 gene in endocrine progenitors and revealed a broad, direct control of endocrine genes by NEUROG3. NEUROG3 not only promotes the development of pancreatic progenitors into islet cells but may also initiate essential endocrine programs for beta cell function.
MOLECULAR METABOLISM
(2021)
Meeting Abstract
Endocrinology & Metabolism
Ramkumar Mohan, Mita Varghese, Simin Abrishami, Kanakadurga Singer
Article
Multidisciplinary Sciences
Yiping Mao, Jacob Schoenborn, Zhihong Wang, Xinqian Chen, Katy Matson, Ramkumar Mohan, Shungang Zhang, Xiaohu Tang, Anoop Arunagiri, Peter Arvan, Xiaoqing Tang
Summary: Abnormal miR-30d levels are closely associated with pancreatic beta-cell loss and dysfunction in type 2 diabetes. Overexpression of miR-30d at high levels leads to diet-induced hyperglycemia and beta-cell dysfunction, accompanied by loss of beta-cell identity, increased alpha-cell identity, and excess plasma glucagon levels. Appropriate miR-30d levels are crucial for maintaining normal beta-cell identity and function.
SCIENTIFIC REPORTS
(2022)
Article
Urology & Nephrology
Xiaoming Fan, Xiaolu Zhang, Lijun C. Liu, Shungang Zhang, Cole B. Pelger, Haroon Y. Lughmani, Steven T. Haller, William T. Gunning III, Christopher J. Cooper, Rujun Gong, Lance D. Dworkin, Rajesh Gupta
Summary: In acute kidney injury, the accumulation of hemopexin in the kidneys worsens kidney injury by increasing hemoglobin deposition on proximal tubular cells to exaggerate hemoglobin-induced cell injury.
KIDNEY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Apurva Lad, Jonathan Hunyadi, Jacob Connolly, Joshua D. Breidenbach, Fatimah K. Khalaf, Prabhatchandra Dube, Shungang Zhang, Andrew L. Kleinhenz, David Baliu-Rodriguez, Dragan Isailovic, Terry D. Hinds, Cara Gatto-Weis, Lauren M. Stanoszek, Thomas M. Blomquist, Deepak Malhotra, Steven T. Haller, David J. Kennedy
Summary: In a murine NAFLD model, chronic exposure to MC-LR led to significant hepatotoxicity, impaired toxin metabolism, and dysregulation of key signaling pathways. Treatment with antioxidants such as NAC and pNaKtide showed improvement in hepatic inflammation and oxidative stress, suggesting the potential reversal of MC-LR metabolism impairments with targeted antioxidant therapy.
Article
Gastroenterology & Hepatology
Florence Blot, Justine Marchix, Miriam Ejarque, Sara Jimenez, Aline Meunier, Celine Keime, Camille Trottier, Mikael Croyal, Celine Lapp, Maxime M. Mahe, Adele De Arcangelis, Gerard Gradwohl
Summary: EECs play a crucial role in regulating energy homeostasis by sensing luminal nutrients and microbial metabolites. The absence of EECs in healthy adult mice leads to weight loss, impaired lipid absorption and metabolism, and altered gut microbiota composition.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Meeting Abstract
Biophysics
Chase Amos, Volker Kiessling, Noah Schenk, Ramkumar Mohan, Catherine A. Doyle, Alex J. B. Kreutzberger, Hongyin Wang, Kandice R. Levental, Ilya Levental, Arun Anantharam, Lukas K. Tamm
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Alina Chapman-Morales, Shreeya Bakshi, Ramkumar Mohan, Mounir Bendahmane, Alan Smrcka, Arun Anantharam
BIOPHYSICAL JOURNAL
(2021)
Article
Endocrinology & Metabolism
Seokwon Jo, Amber Lockridge, Ramkumar Mohan, Nicholas Esch, Regina Schlichting, Neha Panigrahy, Ahmad Essawy, Eric Gustafson, Emilyn U. Alejandro
Summary: The translational factor eIF4G1 is crucial for glucose homeostasis and beta-cell function, as evidenced by its impact on insulin secretion, mitochondrial oxygen consumption rate, and insulin content maintenance in beta-cells.