Article
Cell & Tissue Engineering
Maolin Zhang, Kunimichi Niibe, Takeru Kondo, Phoonsuk Limraksasin, Hiroko Okawa, Xinchao Miao, Yuya Kamano, Masahiro Yamada, Xinquan Jiang, Hiroshi Egusa
Summary: In this study, a new iPSC-Tet/BMP-4 line was established and an efficient approach combining transcriptional activation of BMP-4 and a 3D shaking suspension culture system was developed to differentiate iPSCs into chondrocytes. The culture system could be a useful tool for further investigation of the mechanism of BMP-4 in regulating iPSC differentiation towards the chondrogenic lineage, and should facilitate research in cartilage development, repair, and osteoarthritis.
JOURNAL OF TISSUE ENGINEERING
(2022)
Review
Cell Biology
Vanessa Hall, Katja Maria Sahlgren Bendtsen
Summary: The gut microbiome, gut barrier, and blood-brain barrier are crucial components of the gut-brain axis. Advances in organ-on-a-chip and iPSC technology could allow for more accurate modeling of the physiological functions of the gut-brain axis. It is important to develop non-animal models to study the complex gut-brain axis and its role in various brain disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Isamu Ogawa, Daichi Onozato, Shiho Anno, Hisato Hayashi, Tatsuro Kanaki, Takahiro Iwao, Tamihide Matsunaga
Summary: This study proposes a new culture method for human induced pluripotent stem cell-derived intestinal organoids (HIOs) using low concentrations of natural polysaccharides in a suspension culture. The HIOs cultured in this method showed similarities to living intestines and exhibited pharmacokinetic functions. This method can be widely applied to drug discovery, disease research, and regenerative medicine, overcoming challenges in traditional culture methods.
Review
Biotechnology & Applied Microbiology
Wei Cheng, Chengming Fan, Qing Song, Ping Chen, Hong Peng, Ling Lin, Cong Liu, Bin Wang, Zijing Zhou
Summary: Fibrotic diseases are responsible for a third of global deaths, leading to organ remodeling and dysfunction. Despite the complications of organ transplantation, induced pluripotent stem cell (iPSC)-based therapy shows potential as a treatment option by replacing fibrotic organs. Recent studies have successfully differentiated iPSCs into fibrosis-relevant cell types with anti-fibrotic effects, providing promising precision treatments for organ-specific fibrosis. This review summarizes the potential, advantages, challenges, and future outlook of iPSC-based approaches in treating organ fibrosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Nelio A. J. Oliveira, Handan Sevim
Summary: Dendritic cells are important cells in the immune system, and researchers are looking for efficient methods to obtain dendritic cells from human induced pluripotent stem cells. Despite some challenges, progress has been made and there is potential for clinical applications.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Endocrinology & Metabolism
Takaki Ishida, Michiyo Koyanagi-Aoi, Daisuke Yamamiya, Atsushi Onishi, Katsuya Sato, Keiichiro Uehara, Masato Fujisawa, Takashi Aoi
Summary: A promising protocol for directed differentiation of human induced pluripotent stem cells (hiPSCs) into Leydig-like cells has been reported, showing expression of steroidogenic enzyme genes and specific markers of adult Leydig cells. Functional testosterone secretion from the differentiated cells was confirmed, supporting the potential for hiPSC-derived Leydig-like cells as novel tools for treating Late-onset hypogonadism (LOH) syndrome.
Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Kei Fujiwara, Risa Yamamoto, Tomoya Kubota, Atsutoshi Tazumi, Tomoka Sabuta, Masanori P. Takahashi, Hidetoshi Sakurai
Summary: Researchers have successfully established mature myotubes for skeletal muscle disorder research by purifying hiPSC-derived muscle stem cells. The myotubes obtained displayed spontaneous self-contraction and mature type sodium channel characteristics, achieved by avoiding forced expression of specific genes. This protocol demonstrates high efficiency, high homogeneity, and applicability to drug screening.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Maryam Alowaysi, Robert Lehmann, Mohammad Al-Shehri, Moayad Baadhaim, Hajar Alzahrani, Doaa Aboalola, Asima Zia, Dalal Malibari, Mustafa Daghestani, Khaled Alghamdi, Ali Haneef, Dunia Jawdat, Fahad Hakami, David Gomez-Cabrero, Jesper Tegner, Khaled Alsayegh
Summary: The study focused on establishing a HLA-based iPSC banking system for the Saudi population to overcome the limitations of autologous iPSC-based cellular therapies. By generating HLA-matched iPSC lines, the study demonstrated pluripotency and differentiation potential, covering a portion of the Saudi population. This approach provides a promising step toward expanding stem cell therapies and regenerative medicine in Saudi Arabia.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Surgery
Tomoki Murata, Naoki Hama, Tomoki Kamatani, Akihiro Mori, Ryo Otsuka, Haruka Wada, Ken-ichiro Seino
Summary: This study investigated the immune regulation in transplantation using allogeneic induced pluripotent stem cells (iPSCs). The researchers found that minor antigen mismatches are a risk factor for graft rejection, highlighting the importance of immune regulation. They demonstrated that iPSC-derived hematopoietic stem/progenitor cells (iHSPCs) can induce allograft tolerance, providing a new approach for transplantation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Cell & Tissue Engineering
Yu Zhou, Robert R. A. Wilson, Abinav Udaiyar, Jerri McLemore, Hooman Sadri-Ardekani, Tracy Criswell
Summary: Despite its small size, the pituitary gland plays a crucial role in maintaining the balance of various physiological systems. The development of pituitary organoids could revolutionize fertility treatment and research on neuroendocrine disorders.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Maciej Sulkowski, Marta Kot, Bogna Badyra, Anna Paluszkiewicz, Przemyslaw M. Plonka, Michal Sarna, Dominika Michalczyk-Wetula, Fabio A. Zucca, Luigi Zecca, Marcin Majka
Summary: The study introduces an innovative method for inducing pluripotent stem cells differentiation into melanin-producing cells, generating highly pigmented cells in quantity and quality incomparably higher than previous methods. These melanin-producing cells exhibit characteristics similar to melanocytes and serve as a potential unlimited source for modeling human skin diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Ning Zhai, Xuan Pan, Minhuan Zeng, Lin Xu
Summary: Callus formation in plant tissue culture requires a high auxin concentration. Pluripotency acquisition in the middle cell layer of the callus is crucial for its potential to regenerate organs. This study investigates the development of the middle cell layer in callus tissue from Arabidopsis thaliana hypocotyls.
Review
Dermatology
Satoshi Fukushima, Azusa Miyashita, Haruka Kuriyama, Toshihiro Kimura, Satoru Mizuhashi, Yosuke Kubo, Satoshi Nakahara, Hisashi Kanemaru, Nobuhiro Tsuchiya, Hiroaki Mashima, Rong Zhang, Yasushi Uemura
Summary: Cancer immunotherapy is the primary treatment for unresectable cancers, but it is not a complete cure for all patients. Immune cell therapy, specifically using immune cells generated from induced pluripotent stem cells (iPSCs), shows promise for innovative cancer immunotherapy methods. iPSC-derived dendritic cells (iPS-DCs) can activate T cells, while iPSC-derived macrophages (iPS-MPs) attack cancer cells. iPSCs offer a source for genetic modification and addition of immune functions. Additionally, immortalized iPS-DCs and iPS-MPs could potentially reduce costs through mass production. This review summarizes the achievements and future prospects of cancer research using iPS-DCs and iPS-MPs.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Medicine, General & Internal
Lei Zhang, Ke Pu, Xiaojun Liu, Sarah Da Won Bae, Romario Nguyen, Suyang Bai, Yi Li, Liang Qiao
Summary: Liver diseases are a significant global health concern, often leading to poor survival rates, prompting the need for liver transplantation as a main alternative treatment. The emerging goal of stem cell research is to investigate the molecular mechanisms underlying complex liver diseases. Human induced pluripotent stem cells (hiPSCs) offer a promising alternative approach to treating liver diseases and serve as a potential model for studying complex liver diseases.
FRONTIERS IN MEDICINE
(2021)
Letter
Hematology
Shogo Kasuda, Hideto Matsui, Shiro Ono, Yasunori Matsunari, Kenji Nishio, Midori Shima, Katsuhiko Hatake, Mitsuhiko Sugimoto
Letter
Hematology
Shogo Kasuda, Hideto Matsui, Shiro Ono, Yasunori Matsunari, Kenji Nishio, Midori Shima, Katsuhiko Hatake, Mitsuhiko Sugimoto
Article
Medicine, Legal
Shogo Kasuda, Risa Kudo, Katsuya Yuui, Masatoshi Nakata, Katsuhiko Hatake
AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY
(2016)
Article
Toxicology
K. Yuui, R. Kudo, S. Kasuda, K. Hatake
HUMAN & EXPERIMENTAL TOXICOLOGY
(2016)
Article
Medicine, Legal
Shogo Kasuda, Risa Kudo, Katsuya Yuui, Hiroko Imai, Masatoshi Nakata, Katsuhiko Hatake
Article
Medicine, Legal
Masatoshi Nakata, Shogo Kasuda, Katsulia Yuui, Risa Kudo, Katsuhiko Hatake
Article
Medicine, Legal
Risa Kudo, Katsuya Yuui, Shogo Kasuda, Masatoshi Nakata, Hiroko Imai, Mari Nakanishi, Katsuhiko Hatake
ROMANIAN JOURNAL OF LEGAL MEDICINE
(2017)
Article
Medicine, Legal
Masatoshi Nakata, Shogo Kasuda, Katsulia Yuui, Risa Kudo, Katsuhiko Hatake
Article
Medicine, Research & Experimental
S. Kasuda, R. Kudo, K. Yuui, Y. Sakurai, K. Hatake
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2018)
Article
Cardiac & Cardiovascular Systems
Shogo Kasuda, Yoshihiko Sakurai, Kohei Tatsumi, Tomohiro Takeda, Risa Kudo, Katsuya Yuui, Katsuhiko Hatake
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2018)
Editorial Material
Medicine, Legal
Shogo Kasuda, Risa Kudo, Katsuya Yuui, Takeshi Kondo, Katsuhiko Hatake
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2019)
Article
Veterinary Sciences
Mami Morimoto, Kohei Tatsumi, Katsuya Yuui, Ikuko Terazawa, Risa Kudo, Shogo Kasuda
Summary: This study demonstrated that Convallotoxin (CNT) can induce the expression of tissue factor (TF) in endothelial cells, leading to a hypercoagulable state. The findings contribute to understanding the mechanisms underlying CNT-induced hypercoagulability.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Medicine, Legal
Shogo Kasuda, Risa Kudo, Katsuya Yuui, Takeshi Kondo, Katsuhiko Hatake
ROMANIAN JOURNAL OF LEGAL MEDICINE
(2018)
Article
Hematology
Yasuyuki Urisono, Asuka Sakata, Hideto Matsui, Shogo Kasuda, Shiro Ono, Kiyomi Yoshimoto, Kenji Nishio, Masayuki Sho, Masashi Akiyama, Toshiyuki Miyata, Kazuo Okuchi, Satoshi Nishimura, Mitsuhiko Sugimoto
THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Substance Abuse
Shogo Kasuda, Risa Kudo, Katsuya Yuui, Yoshihiko Sakurai, Katsuhiko Hatake
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)