期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 378, 期 2, 页码 213-217出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.11.035
关键词
p53 promoter; Transcriptional regulation; Ets-1; Oncogene; Cooperative binding; Surface plasmon resonance
资金
- Centre National de la Recherche Scientifique (CNRS)
- Ligue contre le Cancer-Comite du Nord
- CNRS
- Conseil Regional Nord-Pas-de-Calais
- Ministere de la Recherche et de l'Enseignement Superieur
- Ligue Nationale contre le Cancer
Due to its autoinhibition for DNA binding, the Ets-1 transcription factor must interact with partners to enhance its affinity for DNA. In a study on the stromelysin-1 promoter, we showed that Ets-1 binds cooperatively to two Ets-binding sites (EBS) organized in palindrome, thereby circumventing the need for a binding partner to Counteract autoinhibition. This leads to the formation of an Ets-1-DNA-Ets-1 ternary complex necessary for promoter activation. Here we show that Ets-1 also binds cooperatively to the EBS palindrome of the human p53 promoter, despite the presence of a degenerate EBS to which Ets-1 cannot otherwise bind. Transcriptional transactivation through this palindrome fully correlates to Ets-1 binding. Thus, the cooperative binding model that we initially proposed for the stromelysin-1 promoter may be a general mechanism of Ets-1 binding to palindromic EBS separated by 4 bp and a way to counteract binding site degeneracy. (C) 2008 Elsevier Inc. All rights reserved.
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