Article
Microbiology
Qun Wang, Qi Su, Bowen Liu, Yan Li, Wanli Sun, Yanxue Liu, Ruyu Xue, Shuang Chang, Yixin Wang, Peng Zhao
Summary: This study developed a treatment method for combating ALV in vitro culture system by using a combination of AZT and shRNA targeting ALV. The results showed that this method significantly improved antiviral efficacy with low cytotoxicity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dapeng Wang, Jianbo Xiu, Jiangyue Zhao, Junli Luo
Summary: In this study, a new method called miR-AB was developed for simplified, cost-effective, and error-proof production of shRNAmirs. It was successfully applied in loss-of-function experiments for multiple genes at a single-cell level, providing a powerful tool for uncovering the functions of genes in complex biological processes.
Article
Immunology
Jade Pattyn, Greet Hendrickx, Alex Vorsters, Pierre Van Damme
Summary: The coverage of hepatitis B vaccines is increasing, and the effective implementation of vaccination programs has significantly reduced the morbidity and mortality related to hepatitis B.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Pharmacology & Pharmacy
Chunkyu Ko, Jinpeng Su, Julia Festag, Romina Bester, Anna D. Kosinska, Ulrike Protzer
Summary: The study demonstrated the formation of authentic cccDNA in mouse livers after delivery of HBV genomes by rAAV, independent of HBV replication. Intramolecular recombination of the overlength HBV genome was found to be the origin of cccDNA formation in mouse models, with a constant loss of cccDNA molecules over time.
ANTIVIRAL RESEARCH
(2021)
Article
Gastroenterology & Hepatology
Robert L. Kruse, Mercedes Barzi, Xavier Legras, Francis P. Pankowicz, Nika Furey, Lan Liao, Janming Xu, Beatrice Bissig-Choisat, Betty L. Slagle, Karl-Dimiter Bissig
Summary: This study developed a novel transgenic mouse model that allows for excision of HBV from mouse chromosomes to generate a recombinant circular DNA molecule. The model showed regulation of HBV expression and demonstrated the potential for HBV clearance.
Article
Gastroenterology & Hepatology
Wenshi Wang, Florian A. Lempp, Franziska Schlund, Lisa Walter, Charlotte C. Decker, Zhenfeng Zhang, Yi Ni, Stephan Urban
Summary: Research has shown that HDVs from different genotypes replicate with varying efficacies. Variations in HDV genomes and HBV envelope proteins are major determinants of HDV egress and entry efficacy.
JOURNAL OF HEPATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jinmo Koo, Dhandapani Gurusamy, Subba Reddy Palli
Summary: There is limited success in using exogenous small interfering RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double-stranded RNA (dsRNA) is used to induce gene knockdown in insects. In this study, the potency of si/sh RNAs and dsRNA was compared in Colorado potato beetle (CPB) cells. CPB cells showed a highly efficient RNAi response to dsRNA, but si/sh RNAs were inefficient in triggering RNAi in CPB cells.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biophysics
Ru Cheng, Fu Zhu, Min Huang, Qiang Zhang, Hui Hong Yan, Xiao Hui Zhao, Fu Kang Luo, Chun Mei Li, Hui Liu, Gao Lin Liang, Cheng Zhi Huang, Jian Wang
Summary: In this study, a new biosensor named hepatitis virus indicator was developed for the simultaneous detection of HBV and HCV. The biosensor utilized Exo III assisted signal amplification strategy and particle counting technology with a dark-field microscopy, enabling sensitive and selective detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Pharmacology & Pharmacy
Zhongliang Shen, Zixiang Gao, Chenjian Gu, Jingwen Wu, Jinyu Wang, Jiming Zhang, Youhua Xie, Jing Liu
Summary: IL-21 is a key regulator for HBV clearance, and using it as prophylaxis may effectively prevent HBV infection and related hepatic diseases. The experimental study in mouse models showed that prior exposure to IL-21 resulted in HBV clearance and protection against viral persistence, with CD8(+) T cell responses playing a functional role in this process.
ANTIVIRAL RESEARCH
(2021)
Review
Oncology
Ting Wang, Yunhong Yao, Xinrong Hu, Yi Zhao
Summary: The article introduces gene editing interference technology and its potential applications in clinical treatment. It focuses on the mechanisms of CRISPRi, RNAi, and LncRNA interference, and describes their applications and differences in adenocarcinoma research.
Article
Gastroenterology & Hepatology
Julie Lucifora, Dulce Alfaiate, Caroline Pons, Maud Michelet, Ricardo Ramirez, Floriane Fusil, Fouzia Amirache, Axel Rossi, Anne-Flore Legrand, Emilie Charles, Serena Vegna, Rayan Farhat, Michel Rivoire, Guillaume Passot, Nicolas Gadot, Barbara Testoni, Charlotte Bach, Thomas F. Baumert, Anastasia Hyrina, Rudolf K. Beran, Fabien Zoulim, Andre Boonstra, Hildegard Buening, Eloi R. Verrier, Francois-Loic Cosset, Simon P. Fletcher, Anna Salvetti, David Durantel
Summary: This study elucidates the molecular mechanisms underlying how HDV interferes with HBV in patients with chronic coinfection. The researchers found that HDV induces the expression of interferon-stimulated genes (ISGs), and these genes are upregulated in HDV/HBV-coinfected patients. Inhibiting the hepatocyte's response to interferon partially restores the levels of HBV.
JOURNAL OF HEPATOLOGY
(2023)
Article
Microbiology
Le Chang, Ying Yan, Huimin Ji, Huizhen Sun, Xinyi Jiang, Zhuoqun Lu, Lunan Wang
Summary: This study investigated the prevalence of HDV infection among blood donors in China and found a low seroprevalence rate of HDV infection. The risk of HDV transmission through blood transfusion was also found to be low.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Virology
Giacomo Emanuele Maria Rizzo, Giuseppe Cabibbo, Antonio Craxi
Summary: Hepatitis B virus (HBV) infection is associated with the risk of developing hepatocellular carcinoma (HCC), with or without liver cirrhosis, through various mechanisms. The molecular profile of HBV-HCC is constantly being studied, and it is the result of altered molecular pathways, changes in the microenvironment, and DNA damage. Proper management of HBV-related liver disease is crucial for prevention and treatment of HCC.
Review
Immunology
Shuling Wu, Wei Yi, Yuanjiao Gao, Wen Deng, Xiaoyue Bi, Yanjie Lin, Liu Yang, Yao Lu, Ruyu Liu, Min Chang, Ge Shen, Leiping Hu, Lu Zhang, Minghui Li, Yao Xie
Summary: Functional cure is considered achieved when chronic hepatitis B patients achieve HBsAg seroclearance after treatment. Clearing the virus is challenging due to the difficulty in clearing both cccDNA and integrated dslDNA. High levels of HBsAg in the serum lead to immune system failure, making it unable to produce an effective antiviral response. Only less than 1% of chronic hepatitis B patients are cured annually with antiviral treatment. Coinfection with other viruses such as HIV, HCV, and HDV makes it even more difficult to cure. However, it has been found that patients with coinfection have a higher probability of achieving HBsAg seroclearance, especially those with HBV/HIV coinfection with up to 36% 5-year seroclerance rate. This interesting phenomenon is related to the functional reconstruction of the immune system after antiretroviral therapy (ART).
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Po-Yao Hsu, Yu-Ju Wei, Po-Cheng Liang, Jia-Jung Lee, Sheng-Wen Niu, Jiun-Chi Huang, Cheng-Ting Hsu, Tyng-Yuan Jang, Ching- Huang, Yi-Hung Lin, Ming-Yen Hsieh, Meng-Hsuan Hsieh, Szu-Chia Chen, Chia-Yen Dai, Zu-Yau Lin, Shinn-Cherng Chen, Jee-Fu Huang, Jer-Ming Chang, Ming-Lun Yeh, Chung-Feng Huang, Yi-Wen Chiu, Shang-Jyh Hwang, Wan-Long Chuang, Ming-Lung Yu
Summary: Hemodialysis patients are at increased risk of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection. This study found that hemodialysis patients with HCV exhibited a higher burden of comorbidities, especially in ischemic heart diseases, respiratory disorders, and mental/behavioral disorders, compared to patients with HBV and those without viral hepatitis.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Dor Shirin, Noam Peleg, Orly Sneh-Arbib, Michal Cohen-Naftaly, Marius Braun, Tzipora Shochat, Assaf Issachar, Amir Shlomai
DIGESTIVE DISEASES
(2019)
Article
Multidisciplinary Sciences
Yoav Lubelsky, Igor Ulitsky
Article
Multidisciplinary Sciences
Noam Peleg, Orly Sneh Arbib, Assaf Issachar, Michel Cohen-Naftaly, Marius Braun, Amir Shlomai
Article
Multidisciplinary Sciences
Amir Shlomai, Moshe Leshno, Daniel A. Goldstein
Article
Biochemistry & Molecular Biology
Yoav Lubelsky, Yosef Shaul
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Gastroenterology & Hepatology
Noam Peleg, Assaf Issachar, Orly Sneh Arbib, Michal Cohen-Naftaly, Yael Harif, Evelin Oxtrud, Marius Braun, Moshe Leshno, Alon Barsheshet, Amir Shlomai
JOURNAL OF VIRAL HEPATITIS
(2019)
Article
Gastroenterology & Hepatology
Tamar Berger, Iddo Reisler, Tzippy Shochat, Pia Raanani, Eviatar Nesher, Eytan Mor, Anat Gafter-Gvili, Amir Shlomai
DIGESTIVE DISEASES AND SCIENCES
(2020)
Letter
Gastroenterology & Hepatology
Harel Dahari, Amir Shlomai, Scott J. Cotler
JOURNAL OF VIRAL HEPATITIS
(2021)
Article
Biochemistry & Molecular Biology
Yoav Lubelsky, Binyamin Zuckerman, Igor Ulitsky
Summary: The functions of long RNAs depend on their subcellular localization, but the identity of the sequences that dictate this localization and their high-resolution anatomy remain largely unknown. By using massively parallel RNA assays, the study identified the features within the SIRLOIN element and RNA-binding proteins that are required for its activity, providing a roadmap for high-throughput dissection of functional sequence elements in long RNAs.
Article
Multidisciplinary Sciences
Yaara Finkel, Avi Gluck, Aharon Nachshon, Roni Winkler, Tal Fisher, Batsheva Rozman, Orel Mizrahi, Yoav Lubelsky, Binyamin Zuckerman, Boris Slobodin, Yfat Yahalom-Ronen, Hadas Tamir, Igor Ulitsky, Tomer Israely, Nir Paran, Michal Schwartz, Noam Stern-Ginossar
Summary: The study reveals that SARS-CoV-2 infection leads to a global reduction in translation and accelerated degradation of cytosolic cellular mRNAs. It also impairs the translation of transcripts induced in response to infection, probably mediated by inhibition of nuclear mRNA export.
Article
Immunology
Yaara Leibovici-Weissman, Noa Anchel, Eviatar Nesher, Moshe Leshno, Amir Shlomai
Summary: Infections within 180 days post-liver transplantation, particularly surgical site infections and multiple infectious episodes, are significant determinants of long-term survival among patients.
TRANSPLANT INFECTIOUS DISEASE
(2021)
Article
Genetics & Heredity
Neta Degani, Yoav Lubelsky, Rotem Ben-Tov Perry, Elena Ainbinder, Igor Ulitsky
Summary: The highly conserved lncRNAs HOXA-AS3 and HOXB-AS3, transcribed antisense to Hox protein-coding genes, play a role in regulating gene expression in the endoderm lineage and during differentiation of human embryonic stem cells. Their expression patterns are similar across vertebrates, suggesting a potential impact on embryonic development in humans.
Article
Biochemistry & Molecular Biology
Ronen Siman-Tov, Rulla Shalabi, Amir Shlomai, Elad Goldberg, Wesam Essa, Eden Shusterman, Jacob N. Ablin, Michal Caspi, Rina Rosin-Arbesfeld, Ella H. Sklan
Summary: COVID-19 patients are at risk of thrombotic complications due to endothelial activation and tissue damage caused by systemic hyperinflammation. The study found that increased levels of acute-phase protein SAA on platelets of COVID-19 patients may contribute to the observed increase in platelet adhesion. Therefore, reducing inflammation or inhibiting SAA platelet-binding activity could be a valid approach to prevent COVID-19-associated thrombotic complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Noa Shafran, Assaf Issachar, Tzippy Shochat, Inbal Haya Shafran, Michael Bursztyn, Amir Shlomai
Summary: Abnormal liver tests are common in patients with SARS-CoV-2 and influenza infections, reflecting disease severity and associated with worse outcomes, especially in patients with severe liver test abnormalities.
Article
Gastroenterology & Hepatology
Amir Shlomai, Moshe Leshno, Daniel A. Goldstein
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)