Article
Immunology
Mieke Van Ende, Bea Timmermans, Giel Vanreppelen, Sofia Siscar-Lewin, Daniel Fischer, Stefanie Wijnants, Celia Lobo Romero, Saleh Yazdani, Ona Rogiers, Liesbeth Demuyser, Griet Van Zeebroeck, Yuke Cen, Karl Kuchler, Sascha Brunke, Patrick Van Dijck
Summary: In this study, three trehalase enzymes of C. glabrata were characterized, revealing different effects on fungal growth and resistance. Nth1 was found to play a crucial role in oxidative stress resistance.
Article
Plant Sciences
Chuanhong Bian, Yabing Duan, Qian Xiu, Jueyu Wang, Xian Tao, Mingguo Zhou
Summary: In this study, it was discovered that both FgNTH and FgATH are targets of validamycin A (VMA) in Fusarium graminearum, with FgNTH being the main target. Deficiency in FgNTH and FgATH affects the sensitivity to VMA, highlighting their importance in vegetative growth and sexual reproduction. Additionally, the interaction between FgNTH and FgPK, along with the regulation of FgCYP51A and FgCYP51B by FgNTH, contributes to the inhibition of deoxynivalenol (DON) biosynthesis and enhances sensitivity to demethylation inhibitor (DMI) fungicides.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tran Le Cong Huyen Bao Phan, Nathalie Crepin, Filip Rolland, Patrick Van Dijck
Summary: This study reports the discovery of two isoforms of trehalase in Arabidopsis that have different subcellular localization and are involved in the regulation of drought stress tolerance. These isoforms can interact with each other and their activation is dependent on a stress-induced calcium-dependent protein kinase. Both isoforms can mediate ABA-induced stomatal closure, but the cytoplasmic/nuclear isoform is more effective in drought conditions.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhao Wang, Gui-Yun Long, Dao-Chao Jin, Hong Yang, Cao Zhou, Xi-Bin Yang
Summary: The study revealed that SfTre1 and SfTre2 play crucial roles in the growth and development of S. furcifera, with RNA interference affecting their expression and causing malformed phenotypes and high mortality rates.
Article
Agronomy
Maria-Dolores Garcia, Juan-Carlos Arguelles
Summary: The introduction of insecticides and fungicides in agriculture has improved crop yields and quality of life, but their indiscriminate use has led to pest resistance, poisoning, and pollution. Trehalose plays a key role in the pathobiology of insects and fungi, with trehalase inhibitors like Validamycin A emerging as potential antifungal and insecticidal targets.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Li Ren, Yi-Ping Hou, Yuan-Ye Zhu, Fei-Fei Zhao, Ya-Bing Duan, Luo-Yu Wu, Xiao-Xin Duan, Jie Zhang, Ming-Guo Zhou
Summary: This study reveals the roles of FgNth and FgBmh proteins in growth, response to external stresses, and virulence in Fusarium graminearum, and finds that validamycin enhances the interaction between FgNth and FgBmh1.
Article
Veterinary Sciences
Antoine Cournoyer, Annie Deschamps, Liza Bau-Gaudreault, Pascal Dubreuil, Marie-Odile Benoit-Biancamano
Summary: This study validated the use of a portable human glucometer to measure glucose concentration in honeybee hemolymph. The glucometer showed good accuracy and precision at glucose concentrations below 20 mmol/L, and storing hemolymph at -80 degrees C was suitable for long-term conservation of glucose.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Bo Zhang, Yunhui Zhang, Ruobing Guan, Mengfang Du, Xinming Yin, Wenli Zhao, Shiheng An
Summary: This study revealed that PBAN activates the activities of Treh1/2, promotes glycolysis to ensure the supply of acetyl-CoA, and ultimately facilitates sex pheromone biosynthesis and mating behavior in Helicoverpa armigera.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Entomology
Yan Song, Fengming Gu, Weihong Zhou, Ping Li, Fuan Wu, Sheng Sheng
Summary: This study discovered that two trehalase genes, SlTre1 and SlTre2, were highly expressed in the third instar larvae of Spodoptera litura after being parasitized by Meterous pulchricornis. Silencing these genes in parasitized larvae resulted in a significant decrease in trehalase activity and glucose content. Furthermore, the fitness of parasitoid offspring was negatively affected. These findings suggest that parasitoid wasps can regulate trehalase expression in host larvae to support the development of their offspring.
Article
Multidisciplinary Sciences
Vera M. Warmuth, Dirk Metzler, Veronica Zamora-Gutierrez
Summary: Human land modification significantly increases the prevalence of bat-borne coronaviruses, with agriculture, deforestation, and mining being the most significant contributors to the overall impact. This suggests that infection prevalence in wildlife is an important factor underlying the links between human land modification and zoonotic disease emergence.
Article
Immunology
Prasansah Shrestha, Jayram Karmacharya, So-Ra Han, Hyun Park, Tae-Jin Oh
Summary: This study found that there are two types of trehalase genes in Shigella species, with treF gene being prevalent and both treA and treF genes present in the Antarctica isolate S. sp. PAMC28760. This is the first study to discover the presence of two types of trehalase genes in Shigella species, providing insights into how bacteria utilize accessible carbohydrates and the importance of periplasmic trehalase in bacterial virulence.
Article
Biochemistry & Molecular Biology
Guanwang Shen, Jinxin Wu, Ying Lin, Xiaoting Hua, Qingyou Xia, Ping Zhao
Summary: The study revealed that the silkworm ERR is concentrated in the midgut during the last larval stage, and its expression is closely correlated with trehalase. ERR regulates trehalase transcription and promoter activity by directly binding to the core response elements of the trehalase promoter, impacting trehalose metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Yu-Jia Luo, Yan Chen, Xiao-Jing Wang, Si-Tong Wang, Yuan-Yuan Yang, Hong-Xing Xu, Cheng Qu, Yan Wu, Can Li, Shi-Gui Wang, Bin Tang, Su Wang
Summary: It has been found that the trehalase inhibitor validamycin can significantly reduce trehalase activity in fall armyworms, leading to a decrease in glucose and glycogen content. Additionally, it affects the physiological metabolism and development of fall armyworms, increasing larval mortality rate and pupal weight.
ENTOMOLOGIA GENERALIS
(2022)
Article
Entomology
Xiao-Na Shen, Shun-Xia Ji, Wan-Xue Liu, Jian-Ying Guo, Zhi-Chuang Lu, Fang-Hao Wan
Summary: Bemisia tabaci (Hemiptera: Aleyrodidae) shows different responses to temperature stress between its cryptic species, with certain genes playing key roles in regulating cold and heat tolerance. The study suggests that Treh1 gene may be crucial in influencing cold tolerance for the invasive MED species of B. tabaci in China.
ENTOMOLOGIA GENERALIS
(2021)
Article
Entomology
Hai-Zhong Yu, Yu-Ling Huang, Zhan-Jun Lu, Qin Zhang, Hua-Nan Su, Yi-Ming Du, Long Yi, Ba-Lian Zhong, Ci-Xiang Chen
Summary: The study identified three trehalase genes in the Asian citrus psyllid, revealing their important role in regulating chitin metabolism and providing a new target for control of the psyllid.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)