Article
Multidisciplinary Sciences
Sharon A. Sagan, Zahra Moinfar, Carson E. Moseley, Ravi Dandekar, Collin M. Spencer, Alan S. Verkman, Ole Petter Ottersen, Raymond A. Sobel, John Sidney, Alessandro Sette, Mark S. Anderson, Lawrence Steinman, Michael R. Wilson, Joseph J. Sabatino, Scott S. Zamvil
Summary: This study investigates the role of AQP4-specific Th17 cells in the pathogenesis of neuromyelitis optica (NMO). The research shows that AQP4-specific T cells have a high affinity for binding to MHC II, and their clonal expansion is observed in AQP4-deficient mice. Thymic negative selection alone does not account for the tolerance to AQP4 in wild-type (WT) mice. AQP4-specific Th17 cells can cause paralysis followed by recovery, associated with apoptosis of donor T cells. However, these cells can persist and cause persistent paralysis in mice deficient in both T and B cells or lacking T cells only. In contrast, MOG-specific T cells survive and cause sustained disease in WT mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Anatomy & Morphology
Jun Hyeuck Lim, Yong Wook Jung, Hyo Seok Park
Summary: In a rat study, the expression of AQP1 in the choroid plexus and AQP4 in astrocytes of the hippocampal formation was investigated in relation to systemic hyponatremia. The results showed that the expression of AQP1 increased while AQP4 decreased significantly in response to systemic hyponatremia. These changes may play a critical role in the pathogenesis of systemic hyponatremia.
INTERNATIONAL JOURNAL OF MORPHOLOGY
(2021)
Article
Multidisciplinary Sciences
Irene Vorontsova, Alexander Vallmitjana, Belen Torrado, Thomas F. Schilling, James E. Hall, Enrico Gratton, Leonel Malacrida
Summary: Macromolecular crowding and water dynamics play important roles in cellular homeostasis and fate determination. Understanding macromolecular crowding in the lens is crucial for preventing eye diseases such as cataract and presbyopia. This study combines the use of a nanoenvironmental sensor and zebrafish mutants to investigate the spatial regulation of macromolecular crowding and the roles of specific aquaporin proteins in the lens.
Article
Andrology
Jun-Wei Pan, Xiang Zhang, Xing-Wei Jin, Xian-Jin Wang, Wei-Chao Tu, Bao-Xing Huang, Da Xu, Yuan Shao
Summary: This study revealed that hydropenia exacerbated pathological alterations in rats with orthotopic bladder cancer by increasing the expression levels of AQP1, AQP3, and AQP4.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Review
Cell Biology
Zhen Li, Yumeng Quan, Sumin Gu, Jean X. Jiang
Summary: The lens has an internal microcirculatory system that promotes nutrient circulation and waste excretion through channels of aquaporins and connexins. Mutations in these channels can lead to abnormal function and cataract formation. Recent studies have shown that aquaporin and connexin not only have channel functions, but also play a crucial role in lens development and transparency through channel-independent actions. This review focuses on the cell adhesion mediated by aquaporin and connexin and its significance in lens development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ralph Rose, Bjorn Kemper, Albrecht Schwab, Eberhard Schlatter, Bayram Edemir
Summary: Research has shown that primary cultured renal inner medullary collecting duct (IMCD) cells exhibit collective migration behavior, with no differences in migration speed between cells cultivated at either 300 or 600 mosmol/kg. Acute changes in osmolality from 300 to 600 mosmol/kg led to a significant reduction in migration speed, while the reverse led to an increase. Interestingly, AQPs were not localized at the leading edge, with AQP3 disappearing within the first 2-3 rows of cells and AQP4 being enriched at the rear end.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Maria Schroeder-Castagno, Alba Del Rio-Serrato, Andreas Wilhelm, Silvina Romero-Suarez, Patrick Schindler, Cesar Alvarez-Gonzalez, Ankelien-Solveig Duchow, Judith Bellmann-Strobl, Klemens Ruprecht, Maria Hastermann, Gerald Gruetz, Brigitte Wildemann, Sven Jarius, Tanja Schmitz-Huebsch, Friedemann Paul, Carmen Infante-Duarte
Summary: This study aimed to evaluate cell death in neutrophils from Aquaporin-4 (AQP4)-IgG seropositive NMOSD and MOGAD patients. The results showed that neutrophils from AQP4 + NMOSD patients exhibited enhanced survival capacity in response to PMA stimulation, but also increased early apoptotic response.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Cell Biology
H. Login, Johan Palmfeldt, Joleen S. Cheah, Soichiro Yamada, Lene N. Nejsum
Summary: The overexpression of AQP5 in cancers may contribute to carcinogenesis and cancer spread through two independent mechanisms: reduced cell-cell adhesion and increased cell migration capacity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Zihe Huo, Mihai Lomora, Urs Kym, Cornelia Palivan, Stefan G. Holland-Cunz, Stephanie J. Gros
Summary: It was found that AQP1 expression in neuroblastoma cells is up-regulated under hypoxic conditions, and increased AQP1 expression enables cells to form a phenotype associated with migration and increased cell agility. This implies that the hypoxic tumor microenvironment may trigger some tumor cells to transition to a migratory phenotype.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yen-Chieh Chuang, Shu-Yu Wu, Yu-Chuan Huang, Chung-Kan Peng, Shih-En Tang, Kun-Lun Huang
Summary: In this study, the role of aquaporins (AQPs) in macrophage polarization was investigated. The AQP inhibitor mercury chloride (HgCl2) was found to restrict cell volume change, suppress the p38 MAPK/NF kappa B pathway, promote autophagy, and influence macrophage M1 polarization.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Sushanta Debbarma, Ashutosh Ludri, Sudha Saini, Pooja Devi, Gaurav Kumar
Summary: The study found that during summer, the gene expression of AQP1 was higher in nasal turbinate compared to winter, AQP3 was upregulated during summer and significantly downregulated during winter, and AQP4 and AQP5 were upregulated during winter and downregulated during summer in the upper respiratory tract of buffaloes.
BIOLOGICAL RHYTHM RESEARCH
(2022)
Article
Immunology
Elia Sechi, Roberto Zarbo, Maria Angela Biancu, Paola Chessa, Maria Laura Idda, Valeria Orru, Sandra Lai, Stefania Leoni, Paolo Solla
Summary: This article describes an unusual case of a 40-year-old woman with AQP4-IgG-NMOSD who showed prolonged B-cell depletion for nearly five years after a single rituximab reinfusion. In similar rare patients with exceptionally long-lasting B-cell depletion, rituximab reinfusions at fixed pre-planned intervals would result in unnecessary treatment-related risks and health-care expenses.
JOURNAL OF NEUROIMMUNOLOGY
(2021)
Article
Neurosciences
Vanina Netti, Juan Fernandez, Luciana Melamud, Pablo Garcia-Miranda, Gisela Di Giusto, Paula Ford, Miriam Echevarria, Claudia Capurro
Summary: The study reveals that binding of AQP4-IgG to AQP4 in Muller cells leads to partial internalization of AQP4, reducing membrane water permeability and swelling-induced increase in intracellular calcium levels, thereby affecting cell volume regulation and proliferation functions of Muller cells.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Physiology
Catherine Cheng, Junyuan Gao, Xiurong Sun, Richard T. Mathias
Summary: Studies have shown that disruption of Eph-ephrin signaling in mice without EphA2 or ephrin-A5 leads to changes in fiber cell membrane conductance and intracellular voltage despite no obvious alteration in gap junction coupling. This suggests that Eph-ephrin signaling may play a crucial role in maintaining lens transparency and regulating fiber cell conductance.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Cell Biology
Aftab Taiyab, Judith West-Mays
Summary: Injury to the ocular lens disrupts cell-cell and cell-capsule/basement membrane interactions, leading to a series of interconnected signaling events. These events include cell-adhesion and growth factor-mediated signaling pathways that play a crucial role in the induction and progression of epithelial-mesenchymal transition (EMT) and fibrosis of lens epithelial cells. The lens provides a simple and ideal system to study injury-induced signaling pathways associated with EMT and fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)