Article
Biology
Jianfang Huang, Mingcan Zhou, Jianming Chen, Caihuan Ke
Summary: The Haliotis discus hannai is an economically important species in China, but slow growth and small size have caused losses to farmers. Molecular genetic manipulation is the most effective way to breed high-quality abalone with rapid growth rates. This study revealed that the myostatin gene played a critical role in the growth regulation of H. discus hannai and provided a reference for mollusk breeding using molecular markers for growth traits.
Article
Biotechnology & Applied Microbiology
Yanxin Ren, Xueyu Li, Zhonghui Tian, Yan Xu, Ruilin Zhang, Yang Li
Summary: Zebrafish possess evolutionarily conserved innate and adaptive immunity, making them a valuable model organism for studying infection and immunity. This study evaluated the potential of zebrafish to study antiviral RNAi, showing that virus-derived small interfering RNAs are produced after infection with SINVs and NoVs. The slicing activity of human Ago2 can inhibit RNA virus accumulation when expressed in larval zebrafish, suggesting zebrafish as a unique model for studying the antiviral RNAi pathway.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Cell Biology
Vishnu Muraleedharan Saraswathy, Lili Zhou, Anthony R. McAdow, Brooke Burris, Deepika Dogra, Sven Reischauer, Mayssa H. Mokalled
Summary: This study reveals the dynamic expression of mstnb in dorsal spinal cord progenitors in zebrafish after injury. Mstnb mutants show impaired functional recovery, but normal glial and axonal bridging, and an increase in the number of newborn neurons at the lesion site. Treatment with human FGF1 rescues the molecular and cellular phenotypes of mstnb mutants.
Article
Biochemistry & Molecular Biology
Jiajia Zhao, Shuo Yan, Mingshan Li, Lina Sun, Min Dong, Meizhen Yin, Jie Shen, Zhangwu Zhao
Summary: RNA biopesticides are seen as the third revolution in pesticides history due to their precision, efficiency, and eco-friendliness. Through a star polycation nanocarrier-based delivery system, high RNA interference efficiency of npfr was achieved. A highly efficient bacteria-based expression system was used to produce large amounts of dsRNA segments targeting npfr and ampk simultaneously, which were then complexed with nanocarriers to develop a dual-target RNA pesticide. It significantly inhibits larval feeding, growth, and development, outperforming the widely-used azadirachtin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Devora Aharon, Florence L. Marlow
Summary: Zebrafish have become a major model organism for studying vertebrate reproduction due to their high fecundity and external development. The mechanisms of zebrafish sex determination are complex and involve interactions between multiple genes. Comparisons with gene functions in other species can provide insights into the conserved and species-specific mechanisms of zebrafish sex development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Medicine, General & Internal
Moqbel Ali Moqbel Redhwan, M. G. Hariprasad, Suman Samaddar, Sumaia Abdulbari Ahmed Ali Hard, Vidyabhushan Yadav, Apurbo Mukherjee, Rahul Kumar
Summary: This review examines the clinical trials on siRNA published in the last 5 years to understand its benefits, pharmacokinetics, and safety. So far, 55 clinical studies have been conducted on siRNA, showing its effectiveness and safety in treating various diseases. However, there are limitations and uncertainties, such as cellular uptake, precise targeting, and elimination from the body.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Seung Hyun Ahn, Dowoon Gu, Yongjun Koh, Hye-Sook Lee, Sung Wook Chi
Summary: By reanalysing AGO CLIP data in RNA viruses, putative AGO binding sites for siRNAs in the SARS-CoV-2 genome were delineated, and it was found that siRNAs loaded onto AGO can repress target transcripts by acting as miRNAs. Utilizing seed sequences of known antifibrotic miRNAs, potential siRNAs for treating COVID-19 were identified and validated for their activity in lung cell lines.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Yibin Zhu, Chi Zhang, Liming Zhang, Yun Yang, Xi Yu, Jinglin Wang, Qiyong Liu, Penghua Wang, Gong Cheng
Summary: This study reveals how a human-blood-derived microRNA, hsa-miR-150-5p, interferes with the mosquito's antiviral system to facilitate flavivirus infection and transmission. By hijacking the mosquito's RNA interference system, this microRNA suppresses the expression of certain chymotrypsins with potent virucidal activity, impacting the transmission of arboviruses.
Article
Chemistry, Organic
Marta Papis, Camilla Loro, Michele Penso, Gianluigi Broggini, Francesca Foschi
Summary: The synthesis of modified morpholino monomers was achieved through condensation reactions under Lewis acid conditions, utilizing a simple and efficient method to access diversified optically pure morpholino monomers with high yields and anomeric ratios.
ORGANIC CHEMISTRY FRONTIERS
(2022)
Article
Plant Sciences
Pengfei Hao, Han Liu, Baogang Lin, Yun Ren, Lan Huang, Lixi Jiang, Shuijin Hua
Summary: In this study, the metabolites and genes involved in red pigment formation in rapeseed petals were identified. Flavonoids, especially anthocyanins, were found to play an important role in red pigment formation, and BnaA03.ANS was identified as an irreplaceable gene in red-flowered rapeseed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Maritere Uriostegui-Arcos, Steven T. T. Mick, Zhuo Shi, Rufuto Rahman, Ana Fiszbein
Summary: This study presents a strategy to activate gene expression through exon-mediated activation of transcription starts (EMATS) and demonstrates its potential for treating genetic diseases caused by loss of expression of essential genes.
NATURE COMMUNICATIONS
(2023)
Article
Geriatrics & Gerontology
Kazuhiro Maeta, Manal Farea, Hisahide Nishio, Masafumi Matsuo
Summary: A novel splice variant of the human MSTN gene, myostatin-b, was identified in this study. Myostatin-b can inhibit myostatin signaling in a specific manner and enhance myoblast proliferation by regulating the cell cycle. This discovery suggests that myostatin-b could be developed as a natural myostatin inhibitor.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Plant Sciences
Veli Vural Uslu, Athanasios Dalakouras, Victor A. Steffens, Gabi Krczal, Michael Wassenegger
Summary: Transitivity in plants relies on RDR6-mediated dsRNA synthesis and DCL endonuclease activity to reinforce target RNA cleavage through the formation of an RNA-induced silencing complex. This study found that the primary target site influences gene silencing and transitivity, with dsRNA synthesis generally initiated at the 3MODIFIER LETTER PRIME-end of the target RNA.
Article
Chemistry, Multidisciplinary
Ryohei Kajino, Shuichi Sakamoto, Yoshihito Ueno
Summary: In this study, the synthesis of (S)-5'-C-aminopropyl-2'-O-methyladenosine and (S)-5'-C-aminopropyl-2'-O-methylguanosine phosphoramidites was reported, and the properties of small interfering RNAs (siRNAs) containing four (S)-5'-C-aminopropyl-2'-O-methylnucleosides were investigated. The results showed that siRNAs containing (S)-5'-C-aminopropyl-nucleosides at the 3'- and 5'-regions of the passenger strand exhibited good tolerance for RNA interference (RNAi) activity, while the (S)-5'-C-aminopropyl modification in the central region of the passenger strand decreased the RNAi activity. Furthermore, siRNAs containing three or four consecutive (S)-5'-C-aminopropyl-2'-O-methylnucleosides at the 3'- and 5'-regions of the passenger strand showed RNAi activity similar to that of the corresponding 2'-O-methyl-modified siRNAs. Finally, it was observed that (S)-5'-C-aminopropyl modifications effectively improved the serum stability of the siRNAs compared with 2'-O-methyl modifications.
Article
Medicine, Research & Experimental
Candice Bohaud, Jholy De La Cruz, Claudia Terraza, Audrey Barthelaix, Beryl Laplace-Builhe, Christian Jorgensen, Yoan Arribat, Farida Djouad
Summary: This study using zebrafish model and caudal fin resection investigated the crucial role of lactate in macrophage response and fin regeneration. The findings suggest that lactate plays a key role in activating and modulating macrophage phenotype, promoting inflammatory response and tissue regeneration.
Article
Fisheries
Sheng-Han Wu, Hong-Jie Lin, Wen-Fu Lin, Jen-Leih Wu, Hong-Yi Gong
FISH & SHELLFISH IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Sheng-Han Wu, Hsin-Yiu Chou, Ping-Chung Liu, Jen-Leih Wu, Hong-Yi Gong
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Keng-Yu Chiang, Wen-Chun Lin, Tsung-Yu Tsai, Cheng-Wei Lin, Shin-Jie Huang, Ching-Yu Huang, Sheng-Han Wu, Chuian-Fu Ken, Hong-Yi Gong, Jyh-Yih Chen, Jen-Leih Wu
Article
Biology
Te-Hua Hsu, Yu-Ting Chiu, Hung-Tai Lee, Hong-Yi Gong, Chang-Wen Huang
Summary: The development of molecular markers for genetic management and marker-assisted selection in potato grouper was carried out based on transcriptome sequencing, aiming to assist in genetic management and selection of superior growth traits. The transcriptome sequencing yielded 51.34 Gb and 111,490 unigenes, which led to the development of SSR and SNP markers for parentage analysis and functional markers for economic traits.
Article
Environmental Sciences
Esther U. Kadiene, Baghdad Ouddane, Hong-Yi Gong, Jiang-Shiou Hwang, Sami Souissi
Summary: Metal pollution poses a significant challenge to environmental health. This study investigated the multigeneration effects of cadmium on copepod populations, revealing a sex-specific accumulation of cadmium across 10 successive generations. While fecundity increased, males had higher cadmium concentrations than females, and copepod population size decreased under cadmium exposure.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Fisheries
Nguyen Bao Trung, Fan-Hua Nan, Meng-Chou Lee, Jiun-Yan Loh, Hong-Yi Gong, Ming-Wei Lu, Ho Thi Hang, Yu-Lin Lin, Po-Tsang Lee
Summary: TLR18 is expressed in all tissues, with the highest levels in the intestine and the lowest in the liver. It is up-regulated in immune-related organs after bacterial and poly I:C challenges, and in THK cells after LPS stimulation. TLR18 localizes in the intracellular compartment, and interacts with MyD88 and TRIF as adaptors. Constitutively active TLR18 induces the production of various effectors like cytokines and antimicrobial peptides in THK cells.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Keng-Yu Chiang, Ya-Wen Li, Yen-Hsing Li, Shin-Jie Huang, Chih-Lu Wu, Hong-Yi Gong, Jen-Leih Wu
Summary: The study suggests that progranulin A (GrnA) plays a positive regulatory role in liver regeneration process by promoting hepatocyte proliferation, possibly through enhancing HGF/c-met signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Che-Chun Chen, Chang-Wen Huang, Chung-Yen Lin, Chia-Hui Ho, Hong Nhat Pham, Te-Hua Hsu, Tzu-Tang Lin, Rong-Hwa Chen, Shuenn-Der Yang, Chin-I. Chang, Hong-Yi Gong
Summary: This study focuses on improving the tilapia aquaculture industry by developing disease resistance through selective breeding using molecular markers. The researchers established disease-resistance-associated microsatellite markers for tilapia strains farmed in Taiwan and identified genotypes related to survival. A predictive model was also built to calculate the mortality rate of different combinations, showing promising results for the offspring of disease-resistant strains.
Article
Agriculture, Dairy & Animal Science
Pei-Yun Chu, Jia-Xian Li, Te-Hua Hsu, Hong-Yi Gong, Chung-Yen Lin, Jung-Hua Wang, Chang-Wen Huang
Summary: This study investigated the transcriptomic responses of Taiwan tilapia to cold stress, identifying key genes and molecular markers related to cold biological pathways. Energy-related metabolic pathways and nucleotide genotypes showed high correlation with cold tolerance traits. Single nucleotide polymorphism (SNP) genetic variation can assist in selecting and verifying cold-tolerant populations. The study developed molecular markers to improve cold tolerance in Taiwan tilapia through genetic analysis and marker-assisted breeding.
Article
Biology
Te-Hua Hsu, Hung-Tai Lee, Hsueh-Jung Lu, Cheng-Hsin Liao, Hong-Yi Gong, Chang-Wen Huang
Summary: Stock enhancement is an aggressive approach used to replenish depleted wild finfish populations. In Taiwan, stock enhancement projects involving black sea bream have maintained genetic diversity but led to dramatic genetic structure changes within and among wild populations, especially when successful.
Article
Microbiology
Jiali Wang, Xi Chen, Yuxing Zhou, Hua Yue, Ning Zhou, Hongyi Gong, Cheng Tang
Summary: In this study, fecal samples from diarrheal dogs in Southwest China were collected from 2019 to 2021. The study found that 24.8% of the samples were feline parvovirus (FPV)-like viruses instead of canine parvovirus 2 (CPV-2). Genome sequencing and phylogenetic analysis confirmed that these FPV-like strains were closely related to FPV. The study also revealed the wide circulation of FPV-like viruses among dogs in China and their ability to infect dogs efficiently.
VETERINARY MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Wai-Kwan Chu, Shih-Chin Huang, Ching-Fong Chang, Jen-Leih Wu, Hong-Yi Gong
Summary: Transgenic technology and selective breeding have great potential for genetic breeding in both edible and ornamental fish. The CRISPR/Cas9 technology was used to edit the dead end (dnd1) gene in zebrafish, resulting in a loss-of-function mutation that allows for complete infertility control in farmed and transgenic fish.
FRONTIERS IN GENETICS
(2023)
Article
Fisheries
Te-Hua Hsu, Chang-Wen Huang, Hung-Tai Lee, Yi-Hsuan Kuo, Kwang-Ming Liu, Cheng-Hui Lin, Hong-Yi Gong
Article
Environmental Sciences
Esther U. Kadiene, Baghdad Ouddane, Hong-Yi Gong, Min-Sub Kim, Jae-Seong Lee, Yen-Ju Pan, Jiang-Shiou Hwang, Sami Souissi
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Biochemistry & Molecular Biology
Shin-Jie Huang, Chih-Lun Cheng, Jim-Ray Chen, Hong-Yi Gong, Wangta Liu, Jen-Leih Wu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)