Article
Acoustics
JiaYi Li, WeiMing Guo, Fei Yu, Lu Liu, XiaoTing Wang, LvYuan Li, Bing Fang, Lunguo Xia
Summary: The study demonstrated that LIPUS could promote the proliferation and angiogenic differentiation of endothelial cells (ECs) via the activation of the AKT signaling pathway. Additionally, LIPUS could alter the DNA methylation of ECs through the AKT signaling pathway activation, which in turn affected biomolecular changes in the cells.
Article
Food Science & Technology
Ho-Jeong Bang, Mok-Ryeon Ahn
Summary: This study found that pinobanksin exhibits antiangiogenic effect by inducing endothelial cell apoptosis, and it can be developed as an antiangiogenic agent. The mechanism involves inactivation of the Raf/MEK/ERK pathway and activation of apoptotic signaling such as caspase cascades.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Environmental Sciences
Jiajing Cui, Pingwei Wang, Shuqi Yan, Yujun Liang, Dongge Liu, Shuping Ren
Summary: The study aims to explore the role of ferroptosis in endothelial cell dysfunction and its underlying mechanisms.
Article
Biochemistry & Molecular Biology
Kejia Kan, Yifei Mu, Marielle Bouschbacher, Carsten Sticht, Natalia Kuch, Martin Sigl, Nuh Rahbari, Norbert Gretz, Prama Pallavi, Michael Keese
Summary: The study revealed a novel biphasic response of human umbilical vein endothelial cells to blue light illumination. Low fluence blue light promoted cell activities, while high fluence illumination had the opposite effect by upregulating pro-apoptotic signaling pathways. These findings provide insights into photobiomodulation by blue light and potential treatment strategies for angiogenesis-dependent diseases.
Article
Biotechnology & Applied Microbiology
Wei Liu, Yu Feng, Xuehua Wang, Jiaxing Ding, Huili Li, Hongquan Guan, Zhijian Chen
Summary: Acute myocardial infarction (AMI) is a leading cause of human health issues worldwide. Exosome-based therapy, a cell-free approach, shows promise for repairing the ischemic myocardium. This study explores the effects and mechanism of human umbilical vein endothelial cells (HUVECs)-derived exosomes on AMI. The results demonstrate that HUVECs-derived exosomes can significantly improve cardiac function and inhibit cardiomyocyte apoptosis after AMI, possibly through the PI3K/AKT signaling pathway.
Article
Chemistry, Multidisciplinary
Margarita Lopez-Espejel, Marisol Ibarra-Rodriguez, Blanca M. Munoz-Flores, Marcos R. Bahena-Villarreal, Azael A. Cavazos-Jaramillo, Monica D. Garza-Villegas, Cristina Rodriguez-Padilla, Itza E. Luna-Cruz, H. V. Rasika Dias, Juan M. Alcocer-Gonzalez, Victor M. Jimenez-Perez
Summary: The fluorescence imaging technique is widely used in natural and life sciences, but current commercial biomarkers have several disadvantages. In this study, two new fluorescent molecular rotors (SB-B2 and SB-B23) derived from Boron Schiff bases (BOSCHIBAs) were synthesized and characterized. The crystal structure of SB-B23 showed a binuclear complex. Both compounds exhibited viscochromic and thermochromic properties. These low cytotoxicity BOSCHIBAs showed selective fluorescent staining of the endoplasmic reticulum in HeLa cells and HUVECs.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Chin-Feng Hsuan, Thung-Lip Lee, Wei-Kung Tseng, Chau-Chung Wu, Chi-Chang Chang, Tsui-Ling Ko, Ya-Ling Chen, Jer-Yiing Houng
Summary: The study demonstrated that extracts from Glossogyne tenuifolia (GT) can significantly increase nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs), with the root methanol extract showing the most potent effect. The root methanol extract increased NO production by increasing eNOS expression and activating eNOS phosphorylation at serine(1177).
Article
Pharmacology & Pharmacy
Wei Liu, Yanghui Ou, Yumeng Yang, Xuemei Zhang, Liqi Huang, Xiaohua Wang, Buling Wu, Mingcheng Huang
Summary: The study demonstrated that punicalagin has a protective effect on HUVECs activation through an endothelial-mediated mechanism, which may be used in the treatment of various disorders such as cancer, rheumatoid arthritis, and cardiovascular disease. This effect is achieved by reducing adhesion of monocyte cells to HUVECs, decreasing expression of ICAM-1 and VCAM-1, and inhibiting permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs. Additionally, punicalagin suppresses IKK-mediated activation of NF-kappa B signaling and inhibits VEGFR2 activation and downstream p-PAK1.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Genetics & Heredity
Regina L. Binz, Rupak Pathak
Summary: This study investigated the cytogenetic integrity of primary human umbilical vein endothelial cells (HUVECs) and found that approximately 50% of the cells have a deletion of the Xq terminal region. Additionally, a similar Xq deletion was identified in the daughter cell line generated by fusing HUVECs with A549 cells.
Article
Environmental Sciences
Zixuan Liu, Xiaomin Xia, Xuying Lv, Erqun Song, Yang Song
Summary: Iron-bearing nanoparticles (IBNPs) are abundant in particulate matter (PM) and considered hazardous to human health with unclear toxic mode-of-action. Studies show that IBNPs promote ferroptosis, affecting intracellular iron levels, disrupting cell signaling, causing mitochondrial dysregulation, and activating related genes. This highlights the importance of recognizing the ferroptotic activity of IBNPs for assessing the health risks associated with PM.
ENVIRONMENTAL POLLUTION
(2021)
Article
Dermatology
Chen Shen, Ying Jiang, Qiao Li, Chaofan Liu, Feifei Hu, Ming Li
Summary: The study showed that BMP-7 reduced TGF-beta-induced EndoMT in both in vitro and in vivo models of SSc, potentially serving as a promising antifibrotic therapy for SSc.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Pablo C. Caracciolo, Patricia Diaz-Rodriguez, Ines Ardao, David Moreira, Florencia Montini-Ballarin, Gustavo A. Abraham, Angel Concheiro, Carmen Alvarez-Lorenzo
Summary: The study developed bioresorbable small-diameter vascular grafts functionalized with heparin to promote endothelial cell growth and inhibit smooth muscle cells over-proliferation. The tensile properties of the heparin-functionalized vascular grafts resembled those of saphenous veins, indicating potential clinical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Lukasz Gwozdzinski, Joanna Bernasinska-Slomczewska, Pawel Hikisz, Anna Wiktorowska-Owczarek, Edward Kowalczyk, Anna Pieniazek
Summary: In this study, the properties of human varicose vein endothelial cells (HVVEC) and human umbilical vein endothelial cells (HUVEC) were compared. Three bioactive compounds, diosmin, escin, and bromelain, were tested on the cells. The drugs showed different effects on reactive oxygen species and nitric oxide generation in the cells. The study revealed a potential mechanism of action for VV therapy drugs on HVVEC cells.
Article
Biochemical Research Methods
Lisha Mao, Xiaoqi Yuan, Junlei Su, Yaping Ma, Chaofan Li, Hongying Chen, Fugui Zhang
Summary: Under extreme ischemia conditions, human umbilical vein endothelial cells (HUVECs) rely on ischemic tricarboxylic acid (TCA) cycle instead of glycolysis to keep cells alive, but consideration must be given to relieve cell injury.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Yuchen Jia, Xiaoxue Chen, Yajing Chen, Hongxia Li, Xiumei Ma, Wanjin Xing, Kai Zhao
Summary: The Zhenbao pill (ZBP) has been proven to have a protective effect on vascular endothelial cells, reducing cell apoptosis rates, inhibiting ROS production induced by H2O2 exposure, and maintaining mitochondrial membrane potential. ZBP also effectively reduces the accumulation of autophagic puncta, improving cell apoptosis by inhibiting cell autophagy.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Cell Biology
Paola Genevini, Maria Nicol Colombo, Rossella Venditti, Stefania Marcuzzo, Sara Francesca Colombo, Pia Bernasconi, Maria Antonietta De Matteis, Nica Borgese, Francesca Navone
JOURNAL OF CELL SCIENCE
(2019)
Correction
Cell Biology
Paola Genevini, Maria Nicol Colombo, Rossella Venditti, Stefania Marcuzzo, Sara Francesca Colombo, Pia Bernasconi, Maria Antonietta De Matteis, Nica Borgese, Francesca Navone
JOURNAL OF CELL SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Nica Borgese, Javier Coy-Vergara, Sara Francesca Colombo, Blanche Schwappach
Review
Neurosciences
Giuseppe Martano, Elena Monica Borroni, Egesta Lopci, Maria Grazia Cattaneo, Milena Mattioli, Angela Bachi, Ilaria Decimo, Francesco Bifari
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Multidisciplinary Sciences
Hugo J. F. Carvalho, Andrea Del Bondio, Francesca Maltecca, Sara F. Colombo, Nica Borgese
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Nica Borgese
Article
Multidisciplinary Sciences
Alexander Chernorudskiy, Ersilia Varone, Sara Francesca Colombo, Stefano Fumagalli, Alfredo Cagnotto, Angela Cattaneo, Mickael Briens, Mireille Baltzinger, Lauriane Kuhn, Angela Bachi, Andrea Berardi, Mario Salmona, Giovanna Musco, Nica Borgese, Alain Lescure, Ester Zito
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Endocrinology & Metabolism
Maria Grazia Cattaneo, Cristina Banfi, Maura Brioschi, Donatella Lattuada, Lucia M. Vicentini
Summary: The study found that male endothelial cells have a different secretory phenotype compared to female cells under cellular stress, with higher levels of stress-related proteins, apoptosis, and PTX3 secretion. Furthermore, PTX3 plays a crucial role in male endothelial cells' response to apoptosis, while female cells' efferocytosis process seems to be independent of PTX3 expression. These findings may lead to the discovery of sex-specific mechanisms and potential pharmacological targets for endothelial dysfunction and cardiovascular disease.
BIOLOGY OF SEX DIFFERENCES
(2021)
Review
Cell Biology
Cristiana Perrotta, Maria Grazia Cattaneo, Raffaella Molteni, Clara De Palma
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Elisabetta Colombo, Maria Grazia Cattaneo
Summary: 2D cell cultures have been the standard in various applications, but they cannot replicate the complex and dynamic interactions of the tumor microenvironment (TME). Multicellular 3D models are important for studying the spatial dimension, cellular heterogeneity, and molecular networks of the TME.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Nica Borgese, Nicola Iacomino, Sara Francesca Colombo, Francesca Navone
Summary: VAP proteins are essential adaptor proteins of the ER membrane that can recruit interacting partners, with mutations leading to familial ALS. Deficiency of VAPB affects various cell processes, potentially being the main driver of ALS pathogenesis.
Article
Biochemistry & Molecular Biology
Andrea C. Rossetti, Maria Serena Paladini, Cesar Augusto Bruning, Vittoria Spero, Maria Grazia Cattaneo, Giorgio Racagni, Mariusz Papp, Marco A. Riva, Raffaella Molteni
Summary: Neuroinflammation has been shown to play an important role in the pathophysiology of major depressive disorder (MDD) and the mechanism of action of antidepressants. This study investigated the involvement of IL-6 signaling in the therapeutic effects of the antidepressant drug agomelatine in rats exposed to chronic stress. The results revealed that agomelatine was able to restore the negative modulation of IL-6 signaling and improve the anhedonic-like behavior induced by chronic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Cristina Banfi, Maura Brioschi, Lucia M. Vicentini, Maria Grazia Cattaneo
Summary: The human long pentraxin PTX3 has complex regulatory roles in innate immunity, inflammation, and tissue repair. This study compared the proteomic profiles of human endothelial cells (ECs) and found that PTX3 regulates angiogenesis, oxidative stress, and inflammation. Knockdown of PTX3 resulted in an increase in pro-inflammatory markers and a shift to a more oxidative state in ECs. These findings support the idea of a protective function for PTX3 in endothelial homeostasis and cardiovascular biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Arianna Consiglio, Marco Venturin, Sabrina Briguglio, Clara Rossi, Giorgio Grillo, Stefano Bellosta, Maria Grazia Cattaneo, Flavio Licciulli, Cristina Battaglia
Summary: Long non-coding RNAs (lncRNAs) play a key role in regulating cellular senescence. One particular lncRNA associated with senescence, Senescence Associated Long Non-coding RNA (SALNR), has been found to be down-regulated in various cellular models of senescence. However, our investigation reveals that SALNR may not exist as an independent transcript, but rather as a predicted isoform of the HELLS gene. This finding highlights the importance of HELLS in the senescence and aging process.
Article
Biochemistry & Molecular Biology
Nica Borgese, Andres Guillen-Samander, Sara Francesca Colombo, Giulia Mancassola, Federica Di Berardino, Diego Zanetti, Paola Carrera
Summary: Sensorineural age-related hearing loss is a common condition in the elderly, caused by both environmental and genetic factors. In this case report, two sisters were found to have profound hearing loss in their seventies, and genetic sequencing revealed mutations in two genes linked to Usher Syndrome. This suggests that the hearing loss in these sisters may be due to digenic inheritance, as the two genes are integrated in the same functional network involved in stereocilium development and organization.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)