Review
Biochemistry & Molecular Biology
Myung Hee Park, Rajesh Kumar Kar, Siddharth Banka, Alban Ziegler, Wendy K. Chung
Summary: Hypusine, a derivative of lysine formed post-translationally in eukaryotic initiation factor 5A (eIF5A), plays a crucial role in various biological processes. Enzymes involved in hypusine synthesis, deoxyhypusine synthase and deoxyhypusine hydroxylase, exhibit strict specificity towards eIF5A substrates. eIF5A and the hypusine modification pathway are important in human neurodevelopment.
Review
Plant Sciences
Peter Palfi, Laszlo Bakacsy, Henrietta Kovacs, Agnes Szepesi
Summary: Hypusination is a unique posttranslational modification of eIF5A that plays a crucial role in plant growth and stress response. This process, mediated by two enzymes, contributes to the diverse functions of eIF5A isoforms in signal pathways that can be modified effectively.
Review
Medicine, Research & Experimental
Jing-si Guo, Kai-li Liu, Yu -xi Qin, Lin Hou, Ling-yan Jian, Yue-hui Yang, Xin-yang Li
Summary: The discovery of new therapeutic strategies for diseases is crucial for drug research. DHPS plays a critical regulatory role in various diseases, and researchers are increasingly focusing on the development of DHPS inhibitors.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Genetics & Heredity
Alban Ziegler, Katharina Steindl, Ashleigh S. Hanner, Rajesh Kumar Kar, Clement Prouteau, Anne Boland, Jean Francois Deleuze, Christine Coubes, Stephane Bezieau, Sebastien Kury, Isabelle Maystadt, Morgane Le Mao, Guy Lenaers, Benjamin Navet, Laurence Faivre, Frederic Tran Mau-Them, Paolo Zanoni, Wendy K. Chung, Anita Rauch, Dominique Bonneau, Myung Hee Park
Summary: Rare bi-allelic variants in the DOHH gene are associated with a neurodevelopmental disorder, and biochemical evidence confirms insufficient DOHH activity.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Microbiology
Ghulam Jeelani, Tomoyoshi Nozaki
Summary: The eukaryotic translation initiation factor 5A (eIF5A) and deoxyhypusine synthase (DHS) play essential roles in the growth and survival of Entamoeba histolytica, with the two eIF5A isoforms having complementary functions in the parasite's proliferation and survival. The study also highlights the importance of eIF5A and DHS in parasite differentiation, and suggests that the hypusination pathway could be a potential target for drug development against amoebiasis.
Review
Biochemistry & Molecular Biology
Michel Tauc, Marc Cougnon, Romain Carcy, Nicolas Melis, Thierry Hauet, Luc Pellerin, Nicolas Blondeau, Didier F. Pisani
Summary: Eukaryotic initiation factor 5A (eIF5A) has been primarily studied in relation to cell proliferation and cancer development, with isoform A2 being a focus. However, recent research has highlighted the novel molecular features of isoform A1, which is ubiquitously expressed in normal cells, indicating its potential importance in cellular functions and organ homeostasis. Additionally, eIF5A's involvement in various physiological processes and its potential as a drug target for conditions like organ transplantation and ischemic injuries have opened up new clinical possibilities.
CELL AND BIOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Aristeidis Panagiotis Sfakianos, Rebecca Mallory Raven, Anne Elizabeth Willis
Summary: Protein synthesis dysregulation in cancer provides a promising therapeutic target. The elongation factor eIF5A, involved in protein elongation and with multiple functions in cellular processes, is being considered as a potential target for cancer treatment. Inhibitors of eIF5A are under development as anti-cancer agents.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Aiyada Aroonsri, Chayaphat Wongsombat, Philip Shaw, Siegrid Franke, Jude Przyborski, Annette Kaiser
Summary: Treatment of protozoal infections is challenging due to drug shortages and increasing resistance, but recent progress has been made through parasite genome sequencing and validation of new targets. Posttranslational modification of a novel amino acid, hypusine, has potential as a drug target. Experimental results with human dhs inhibitors in Plasmodium show promising antimalarial activity, but further research is needed.
Article
Biochemistry & Molecular Biology
Rebecca Chase, June Bryan de la Pena, Patrick R. Smith, Jennifer Lawson, Tzu-Fang Lou, Alexander D. Stanowick, Bryan J. Black, Zachary T. Campbell
Summary: Nociceptors are sensory neurons that play a crucial role in pain sensation. A recent study explored the potential of using sensory neurons derived from human induced pluripotent stem cells (hiPSCs) as a model for nociceptor research. The study compared the gene expression profiles of hiPSC-derived sensory neurons with mouse dorsal root ganglion (DRG) tissues and found similarities in key pain-related transcripts. Additionally, the study identified a translation factor called eIF5A as a potential target for inflammation-associated pain. Inhibition of eIF5A activity showed promising results in preventing pain hypersensitivity in vivo and reducing hiPSC activity in vitro.
Article
Immunology
Rocio Seoane, Yessica Y. Llamas-Gonzalez, Santiago Vidal, Ahmed El Motiam, Yanis Hichem Bouzaher, Danae Fonseca, Rosa Farras, Adolfo Garcia-Sastre, Jose Gonzalez-Santamaria, Carmen Rivas
Summary: Active hypusine-modified eIF5A plays a critical role in cell proliferation, differentiation, and immune response. Virus infection and double-stranded RNA viral mimic stimulation induce hypusination of eIF5A. Activation of eIF5A is crucial for the replication of various RNA viruses. Inhibiting hypusination of eIF5A may induce upregulation of endoplasmic reticulum stress marker proteins and interferon, leading to broad-spectrum antiviral activity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Hongde Li, Bo-Kuan Wu, Mohammed Kanchwala, Jing Cai, Li Wang, Chao Xing, Yonggang Zheng, Duojia Pan
Summary: Metabolic reprogramming is crucial in oncogene-induced tumorigenesis, and the regulation between YAP/TAZ and polyamine plays a significant role in tumor growth.
NATURE CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karina D. Pereira, Leticia Tamborlin, Tanes de Lima, Silvio R. Consonni, Leonardo R. Silveira, Augusto D. Luchessi
Summary: The study highlights the essential role of the alternative human isoform A of eIF5A in controlling mitochondrial processes, with its depletion affecting gene expression related to mitochondria and leading to apoptosis in cells.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Cell Biology
Nicolas Melis, Isabelle Rubera, Sebastien Giraud, Marc Cougnon, Christophe Duranton, Mallorie Poet, Gisele Jarretou, Raphael Thuillier, Laurent Counillon, Thierry Hauet, Luc Pellerin, Michel Tauc, Didier F. Pisani
Summary: Through kidney transplantation, ischemia/reperfusion induces tissular injury due to energy shortage, oxidative stress, and endoplasmic reticulum (ER) stress. We found that the specific inhibitor of eukaryotic Initiation Factor 5A (eIF5A) hypusination, GC7, protects kidney cells by modulating unfolded protein response (UPR) pathways. GC7 pretreatment enhances xbp1 splicing and BiP expression while reducing eIF2 alpha phosphorylation and ATF6 nuclear level, indicating a specific modulation of UPR pathways. Inhibition of xbp1 splicing reverses the protective effect of GC7 against anoxia, demonstrating the crucial role of eIF5A hypusination inhibition in UPR modulation.
Article
Multidisciplinary Sciences
Thomas C. J. Tan, Van Kelly, Xiaoyan Zou, David Wright, Tony Ly, Rose Zamoyska
Summary: This study reveals that the elongation factor eIF5a in naive CD8(+) T cells is dynamically regulated upon activation through post-translational modification, and it facilitates the translation of specific proteins. It is essential for long-term survival of effector CD8(+) T cells, and controls the production of proteins important for proliferation and effector functions.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Leah R. Padgett, Morgan A. Robertson, Emily K. Anderson-Baucum, Craig T. Connors, Wenting Wu, Raghavendra G. Mirmira, Teresa L. Mastracci
Summary: Deletion of either Dhps or Eif5a in murine pancreas results in the absence of acinar cells, indicating a crucial role of DHPS and eIF5A in cellular development. The catalytic activation of eIF5A by DHPS is essential for mRNA translation and protein production that influence cellular function. This study highlights the previously underappreciated importance of DHPS and eIF5A in protein synthesis for cellular development and function.
Article
Cell Biology
Monika Sramkova, Andrius Masedunskas, Roberto Weigert
HISTOCHEMISTRY AND CELL BIOLOGY
(2012)
Article
Multidisciplinary Sciences
Andrius Masedunskas, Monika Sramkova, Laura Parente, Katiuchia Uzzun Sales, Panomwat Amornphimoltham, Thomas H. Bugge, Roberto Weigert
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Biotechnology & Applied Microbiology
Lucyna Kapka-Slcrzypczak, Magdalena Czajka, Krzysztof Sawicki, Magdalena Matysiak-Kucharek, Alena Gabelova, Monika Sramkova, Helena Bartyzel-Lechforowicz, Marcin Kruszewski
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2019)
Review
Biotechnology & Applied Microbiology
Marcin Kruszewski, Katarzyna Sikorska, Sylwia Meczynska-Wielgosz, Agnieszka Grzelak, Monika Sramkova, Alena Gabelova, Lucyna Kapka-Skrzypczak
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2019)
Review
Biotechnology & Applied Microbiology
Alena Gabelova, Katarina Kozics, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Monika Sramkova
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2019)
Article
Biotechnology & Applied Microbiology
Monika Sramkova, Katarina Kozics, Vlasta Masanova, Iveta Uhnakova, Filip Razga, Veronika Nemethova, Petra Mazancova, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Marta Novotova, Victor F. Puntes, Alena Gabelova
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2019)
Review
Biotechnology & Applied Microbiology
Goran Gajski, Bojana Zegura, Carina Ladeira, Bertrand Pourrut, Cristian Del Bo', Matjaz Novak, Monika Sramkova, Mirta Milic, Kristine Bjerve Gutzkow, Solange Costa, Maria Dusinska, Gunnar Brunborg, Andrew Collins
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2019)
Review
Biotechnology & Applied Microbiology
Goran Gajski, Bojana Zegura, Carina Ladeira, Matjaz Novak, Monika Srarnkova, Bertrand Pourrut, Cristian Del Bo', Mirta Milic, Kristine Bjerve Gutzkow, Solange Costa, Maria Dusinska, Gunnar Brunborg, Andrew Collins
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2019)
Article
Biochemical Research Methods
Peter Moller, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, Mirta Milic, Goran Gajski, Solange Costa, Joao Paulo Teixeira, Cristiana Costa Pereira, Maria Dusinska, Roger Godschalk, Gunnar Brunborg, Kristine B. Gutzkow, Lisa Giovannelli, Marcus S. Cooke, Elke Richling, Blanca Laffon, Vanessa Valdiglesias, Nursen Basaran, Cristian Del Bo', Bojana Zegura, Matjaz Novak, Helga Stopper, Pavel Vodicka, Sona Vodenkova, Vanessa Moraes de Andrade, Monika Sramkova, Alena Gabelova, Andrew Collins, Sabine A. S. Langie
Article
Chemistry, Multidisciplinary
Katarina Kozics, Monika Sramkova, Kristina Kopecka, Patricia Begerova, Alena Manova, Zora Krivosikova, Zuzana Sevcikova, Aurelia Liskova, Eva Rollerova, Tibor Dubaj, Victor Puntes, Ladislava Wsolova, Peter Simon, Jana Tulinska, Alena Gabelova
Summary: The study demonstrates potential toxic effects of tissue accumulation of PEG-AuNPs, primarily manifesting as cellular changes in the liver and spleen, but with unclear long-term effects.
Article
Cell Biology
Andrea Soltysova, Patricia Begerova, Kristina Jakic, Katarina Kozics, Monika Sramkova, Eckart Meese, Bozena Smolkova, Alena Gabelova
Summary: The unique physicochemical properties of inorganic nanoparticles make them an exciting tool in diagnosis and disease management. However, their difficulty in degradation and excretion may lead to unintended accumulation in tissues and adverse health effects. This study provides a methylome-transcriptome framework for assessing the chronic effects of commonly used inorganic nanoparticles in human kidney cells, revealing deregulated genes related to immune response, DNA damage, and cancer pathways.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tibor Dubaj, Katarina Kozics, Monika Sramkova, Alena Manova, Neus G. Bastus, Oscar H. Moriones, Yvonne Kohl, Maria Dusinska, Elise Runden-Pran, Victor Puntes, Andrew Nelson, Alena Gabelova, Peter Simon
Summary: Data for assembling a physiologically-based pharmacokinetic (PBPK) model for nanoparticles (NPs) are relatively scarce, leading to a trend of extrapolating results from in vitro and in silico studies to in vivo nanoparticle hazard and risk assessment. To evaluate the reliability of this approach, a pharmacokinetic study was conducted using the same polyethylene glycol-coated gold nanoparticles (PEG-AuNPs) in vitro and in vivo. The study found notable differences in the internalized amount of Au between individual cell lines and corresponding tissues in vivo, indicating the caution needed when extrapolating in vitro data to predict the in vivo NP burden and response to exposure.
Article
Biochemistry & Molecular Biology
Yvonne Kohl, Nicola William, Elisabeth Elje, Nadine Backes, Mario Rothbauer, Annamaria Srancikova, Elise Runden-Pran, Naouale El Yamani, Rafi Korenstein, Lea Madi, Alexander Barbul, Katarina Kozics, Monika Sramkova, Karen Steenson, Alena Gabelova, Peter Ertl, Maria Dusinska, Andrew Nelson
Summary: This study compared the performance and output of an electrochemical phospholipid membrane platform with cell-based toxicity testing methods using various toxicants. The results showed that the membrane sensor and cell-based systems provided similar toxicity rankings when acute cell viability was the endpoint. However, a different toxicity ranking was observed when colony forming efficiency or DNA damage was used as the endpoint. The study demonstrates the potential of electrochemical membrane-based sensors for rapid preliminary toxicity screening.
BIOELECTROCHEMISTRY
(2023)
Article
Oncology
K. Kozics, A. Srancikova, E. Sedlackova, E. Horvathova, M. Melusova, V. Melds, Z. Krajcovicova, M. Sramkova
Article
Cell Biology
Monika Sramkova, Laura Parente, Timothy Wigand, Myo-Pale Aye, Akiko Shitara, Roberto Weigert
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)