Article
Genetics & Heredity
Takahiro Sano, Koki Ueda, Keiji Minakawa, Tsutomu Mori, Yuko Hashimoto, Haruhiko Koseki, Yasuchika Takeishi, Kazuhiko Ikeda, Takayuki Ikezoe
Summary: This study generated Uhrf2(-/-) mice to investigate the role of UHRF2 deletion in hematopoiesis. The results showed that the absence of UHRF2 did not affect blood counts, bone marrow findings, and spleen weights. However, the proportions of Uhrf2(-/-) cells were decreased compared to Uhrf2(+/+) cells in competitive repopulation assays, indicating a potential regulatory role of UHRF2 in hematopoiesis.
Article
Cell Biology
Wanbo Tang, Jian He, Tao Huang, Zhijie Bai, Chaojie Wang, Haizhen Wang, Ruichuang Yang, Yanli Ni, Jun Hou, Junliang Wang, Jie Zhou, Yingpeng Yao, Yandong Gong, Siyuan Hou, Bing Liu, Yu Lan
Summary: Using gene knockin strategy, a new Hlf-tdTomato reporter mouse model was developed that revealed the importance of Hlf expression in the generation of functional HSCs from early pre-HSCs. The construction of a novel Hlf-CreER mouse model enabled genetic lineage tracing showing the contribution of early cells to multi-lineage hematopoiesis in the bone marrow. This study provides valuable tools to specifically trace the fate of emerging HSCs during development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Leonard Herault, Mathilde Poplineau, Adrien Mazuel, Nadine Platet, Elisabeth Remy, Estelle Duprez
Summary: By analyzing 15,000 young and aged HSC transcriptomes, the study identified 15 groups of HSCs showing rare and new specific abilities that change with age. This established a new reference map of HSC differentiation and revealed a potential mechanism that delays differentiation in aged HSCs, potentially contributing to age-related hematologic diseases.
Review
Hematology
Laura R. Goldberg
Summary: Extracellular vesicles (EVs) play a critical role in modulating hematopoiesis within the bone marrow microenvironment and may be key mediators of HSC aging. Studies have shown their involvement in numerous age-related biologic processes and diseases, influencing both normal and malignant hematopoiesis.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Xiangjun Zeng, Xia Li, Mi Shao, Yulin Xu, Wei Shan, Cong Wei, Xiaoqing Li, Limengmeng Wang, Yongxian Hu, Yanmin Zhao, Pengxu Qian, He Huang
Summary: This study identified markers of apoptosis-biased and inflammatory aged HSCs, common differentially expressed genes between young and aged HSCs, and functional enrichment analysis showed involvement in the cell cycle and TNF signaling pathway. The Skp2-induced signaling pathway was found to contribute to rapid transition through G1 phase in aged HSCs, and inflammatory genes in the bone marrow microenvironment were increased in HSC aging. Inflammatory cytokines promoted cellular senescence, but blockade of the inflammatory pathway rejuvenated aged HSC functions and increased B cell output.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Claudia Morganti, Keisuke Ito
Summary: Mitochondrial dysfunction and stem cell exhaustion are characteristic features of aging, particularly in the hematopoietic system. Understanding the key mechanisms of HSC aging, including mitochondrial ROS production and alterations in metabolism, could lead to the identification of new therapeutic targets to prevent, delay, or reverse aspects of the aging process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Takeshi Fujino, Shuhei Asada, Susumu Goyama, Toshio Kitamura
Summary: This review discusses the causes of hematopoietic stem cell aging, including both intrinsic and extrinsic factors. Epigenetics and inflammation have been found to be involved in the connection between HSC aging, clonality, and oncogenesis. Clarifying the mechanisms of HSC aging has accelerated the development of therapeutic interventions for rejuvenating HSCs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Cell Biology
Sydney Treichel, Marie-Dominique Filippi
Summary: Hematopoietic stem cells have the ability to self-renew or differentiate into various blood cell lineages. The cell fate decisions of self-renewal or differentiation must be tightly controlled to prevent diseases like bone marrow failure or leukemia. Studies have shown that the cell cycle length plays a crucial role in hematopoietic stem cell fate. Cell cycle kinetics, divisional history, asymmetric cell divisions, metabolic and organelle activity all have an impact on hematopoietic stem cell fate decisions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Geovanny Genaro Reivan Ortiz, Yasaman Mohammadi, Ahmad Nazari, Mehrnaz Ataeinaeini, Parisa Kazemi, Saman Yasamineh, Bashar Zuhair Talib Al-Naqeeb, Haider Kamil Zaidan, Omid Gholizadeh
Summary: Aging is a biological process that leads to a decrease in the organism's standard of living due to time-related cellular and functional impairments. Recent advances in aging investigation have revealed that the rate of senescence can be regulated through genetic pathways and biological processes. Hematopoietic stem cells (HSCs) maintain blood generation throughout an organism's lifetime but are also affected by the senescence process, resulting in a decline in their capabilities. MicroRNAs (miRNAs) play a crucial role in controlling HSCs and can modulate senescence and age-associated diseases.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Genetics & Heredity
Mohadese Hashem Boroojerdi, Vahid Hosseinpour Sarmadi, Maryam Maqbool, King-Hwa Ling, Pooria Safarzadeh Kozani, Pouya Safarzadeh Kozani, Rajesh Ramasamy
Summary: This study elucidates the regulatory mechanisms of mesenchymal stem cells (MSCs) on the proliferation of hematopoietic stem cells (HSCs), providing a potential approach for obtaining larger quantities of HSCs in vitro without the need for manipulating their differentiation potential.
Article
Cell & Tissue Engineering
Han-Sol So, Min-Guk Kim, Jeong-Chae Lee, Sung-Ho Kook
Summary: The study found that using glucose oxidase (GO) can effectively promote the mobilization of hematopoietic stem progenitor cells (HSPCs) from the bone marrow to peripheral blood, even in animal models. Through combined use with granulocyte colony-stimulating factor (G-CSF) and/or AMD3100, the mobilization of HSPCs can be greatly enhanced.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Hematology
Christina M. Termini, Amara Pang, Michelle Li, Tiancheng Fang, Vivian Y. Chang, John P. Chute
Summary: The discovery of Syndecan-2 as a novel surface marker and regulator of hematopoietic stem cell (HSC) function has improved the understanding of HSC identity and their capacity for repopulation. Syndecan-2 expression is significantly increased in long-term HSCs compared to differentiated hematopoietic cells. Isolation of bone marrow cells based on Syndecan-2 expression results in a higher enrichment of HSCs with superior in vivo repopulating capacity, compared to conventional selection methods. Syndecan-2 regulates HSC repopulating capacity through control of Cdkn1c expression and HSC quiescence.
Article
Engineering, Environmental
Govinda Bhattarai, Hyun-Jaung Sim, Han-Sol So, Jeong-Chae Lee, Sung-Ho Kook
Summary: Research has mainly focused on the negative impacts of PM2.5 on respiratory, brain, immune, and metabolic diseases, with little knowledge about its effects on hematopoietic stem cell (HSC) fate. This study investigated the effects of atmospherically relevant PM2.5 on newborn hematopoietic stem progenitor cells (HSPCs), revealing increased oxidative stress and inflammasome activation in the lungs and bone marrow of PM2.5-exposed mice.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Virology
Lindsey B. Crawford, Meaghan H. Hancock, Hillary M. Struthers, Daniel N. Streblow, Andrew D. Yurochko, Patrizia Caposio, Felicia D. Goodrum, Jay A. Nelson
Summary: This study demonstrates that CD34(+) progenitor cells derived from embryonic stem cells can serve as a model for HCMV latency, and the virus may either latently or persistently infect specific subpopulations of CD34(+) cells.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Momoko Yoshimoto, Astrid Kosters, Samuel Cornelius, Noemi Valiente, Haizi Cheng, Augusto Latorre, Chika Nishida, Eliver E. B. Ghosn, Michihiro Kobayashi
Summary: Recent advances in developmental immunology have shown that mast cells (MCs) and other innate immune cells originate from hematopoietic stem cells (HSCs) independently. The production of MCs mostly happens before the emergence of HSCs in the aorta-gonad-mesonephros (AGM) region and is not regenerated by adult bone marrow (BM) long-term HSCs. However, it was unknown whether the first emerging HSCs in the AGM or fetal liver (FL) have the ability to regenerate MCs. This study found that HSCs from E11.5 AGM and E12.5 FL can efficiently repopulate MCs, while HSCs from >= E14.5 FL fail to do so. Additionally, the majority of MCs were found to originate from endothelial cells (ECs). These findings challenge the stem cell theory in hematology and EC-derived mast cells may play a role in the pathogenesis of postnatal mast cell disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)