Article
Multidisciplinary Sciences
Moritz Hunkeler, Cyrus Y. Jin, Eric S. Fischer
Summary: Tightly regulating apoptosis is crucial for the development of multicellular organisms and preventing diseases like cancer and neurodegeneration. Caspase activation is key to apoptosis, and inhibitor of apoptosis proteins (IAPs) are important for restraining caspase activity and can be targeted therapeutically. IAPs, in turn, are regulated by proapoptotic factors derived from mitochondria like SMAC and HTRA2. By studying the structures of human baculoviral IAP repeat-containing protein 6 (BIRC6) bound to SMAC, caspases, and HTRA2 through cryo-electron microscopy, we gain a molecular understanding of how BIRC6 inhibits caspase activity and how it is released by SMAC.
Article
Biochemistry & Molecular Biology
Panagis Polykretis, Enrico Luchinat
Summary: XIAP is a multi-functional protein overexpressed in cancer cells, acting as a direct caspase inhibitor to regulate apoptosis and becoming an important target for cancer treatment. The pro-apoptotic protein Smac is considered a master regulator of apoptosis in mammals, and the development of Smac mimetic compounds for inhibiting XIAP activity has shown limited efficacy. This study provides the first bio-physical characterization of the interaction between full-length XIAP and Smac, offering important information for the development of more effective XIAP inhibitors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Varda Shoshan-Barmatz, Tasleem Arif, Anna Shteinfer-Kuzmine
Summary: Apoptosis is a regulated cell death process that maintains tissue integrity. It involves two major pathways: the extrinsic pathway, activated by external signals, and the intrinsic pathway, activated by DNA damage or stress. However, apoptotic proteins also have non-death-related functions, particularly in non-cancer cells. This review highlights the unconventional roles of apoptotic proteins, focusing on VDAC1 and SMAC/Diablo, mitochondrial proteins that are overexpressed in cancer cells.
Article
Cell Biology
Ruven Wilkens, Anne Hoffrichter, Karolina Kleinsimlinghaus, Bettina Bohl, Carolin Haag, Nadja Lehmann, Malin Schmidt, Elena Munoz Perez-Vico, Julia Wangemann, Klara Franziska Rehder, Sandra Horschitz, Georg Koehr, Julia Ladewig, Philipp Koch
Summary: The study reveals that human neurons develop a complex and redundant anti-apoptotic signaling network during maturation, which enhances their resilience against cellular stress and reduces the risk of accidental cell death.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Petra Kuliskova, Lucie Vasatkova, Iva Slaninova
Summary: This study demonstrates that six quaternary benzophenanthridine alkaloids (QBAs) can overcome drug resistance caused by defects in cell death signaling pathways and identifies a novel mechanism of action.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Agnieszka Pluta, Tadeusz Robak, Barbara Cebula, Agata Majchrzak, Piotr Pluta, Kamil Brzozowski, Konrad Stepka, Anna Szmigielska-Kaplon, Olga Grzybowska-Izydorczyk, Magdalena Czemerska, Piotr Smolewski, Agnieszka Wierzbowska
Summary: The expression of NE-kappa B and Smac/DIABLO proteins in AML patients can impact patient outcomes, with a negative correlation observed between Smac/DIABLO and NE-kappa B levels. More favorable outcomes were associated with younger age, higher Smac/DIABLO expression, and lower NE-kappa B levels.
ARCHIVES OF MEDICAL SCIENCE
(2021)
Review
Chemistry, Multidisciplinary
Yung-Chieh Chang, Chun Hei Antonio Cheung
Summary: This article reviews the anti-cancer potency and novel molecular targets of Smac mimetics, highlighting their ability to induce apoptosis, arrest cell cycle, induce necroptosis, and trigger an immune response. Clinical trials have evaluated the safety and effectiveness of Smac mimetics, with promising results when combined with chemotherapy compounds. Recent studies have uncovered new anti-cancer mechanisms of Smac mimetics, indicating further exploration is needed for potential modification and combination therapies.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Chemical
Yung-Chih Kuo, I-Shan Yang, Rajendiran Rajesh
Summary: By encapsulating BV6 and GDC0152 in solid lipid nanoparticles with surface transferrin and folic acid modifications, the efficacy of these two Smac mimetics in glioblastoma multiforme treatment can be enhanced, allowing for targeted drug delivery and release in GBM cells.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Oncology
Swaroop Kumar Pandey, Avijit Paul, Anna Shteinfer-Kuzmine, Ran Zalk, Uwe Bunz, Varda Shoshan-Barmatz
Summary: SMAC/Diablo, known as a pro-apoptotic protein, is found to be overexpressed in several cancer types, playing a noncanonical role in regulating lipid synthesis and cancer cell proliferation. It interacts with mitochondrial PSD to inhibit its catalytic activity, affecting the synthesis of PE and ultimately impacting cancer cell growth. Additionally, SMAC/Diablo can also regulate nuclear activities by influencing the levels of nuclear PE as a signaling molecule. Targeting the SMAC-PSD interaction as a potential therapeutic strategy for cancer treatment is suggested by the findings of this study.
MOLECULAR ONCOLOGY
(2021)
Article
Biology
David Cerna, Bora Lim, Yusuf Adelabu, Stephen Yoo, Donna Carter, Ahmed Fahim, Yasuhiro Mitsuuchi, Beverly A. Teicher, Eric Bernhard, C. Norman Coleman, Naoko Takebe, Mansoor M. Ahmed
Summary: Research has shown that birinapant can enhance the radiosensitivity of glioblastoma cell lines, with particularly significant effects observed in the U-251 cell line. The combination treatment of birinapant and radiation therapy demonstrated tumor growth delay and a survival benefit in both in vitro and in vivo models, warranting further investigation into this potential treatment approach.
RADIATION RESEARCH
(2021)
Article
Cell Biology
Hamza Ali, Ramon Caballero, Simon X. M. Dong, Niranjala Gajnayaka, Agatha Vranjkovic, Duale Ahmed, Salma Iqbal, Angela M. Crawley, Jonathan B. Angel, Edana Cassol, Ashok Kumar
Summary: The study shows that human macrophage subsets respond differently to cell death mechanisms and exhibit varying resistances to certain drugs, suggesting potential for targeted therapies in various inflammatory diseases.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Julian F. Ehrmann, Daniel B. Grabarczyk, Maria Heinke, Luiza Deszcz, Robert Kurzbauer, Otto Hudecz, Alexandra Shulkina, Rebeca Gogova, Anton Meinhart, Gijs A. Versteeg, Tim Clausen
Summary: Inhibitor of apoptosis proteins (IAPs) bind to pro-apoptotic proteases, keeping them inactive and preventing cell death. The atypical ubiquitin ligase BIRC6 is the only essential IAP, additionally functioning as a suppressor of autophagy. Cryo-electron microscopy structures showed that BIRC6 forms a megadalton crescent shape that arcs around a spacious cavity containing receptor sites for client proteins. Multivalent binding of SMAC obstructs client binding, impeding ubiquitination of both autophagy and apoptotic substrates. Based on these data, we discuss how the BIRC6/SMAC complex can act as a stress-induced hub to regulate apoptosis and autophagy drivers.
Article
Biochemistry & Molecular Biology
Zeenat Ismail, Jean Dam, Clement Penny, Charles B. de Koning, Leonie Harmse
Summary: Copper-imidazo[1,2-a]pyridines show potential as drug candidates against leukemia cells by activating the apoptotic pathway. The response of apoptotic regulatory proteins to these compounds is inconsistent between different cell lines, indicating the effect is modulated by molecular signatures of each cell line.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Larissa Dietz, Cara J. Ellison, Carlos Riechmann, C. Keith Cassidy, F. Daniel Felfoldi, Adan Pinto-Fernandez, Benedikt M. Kessler, Paul R. Elliott
Summary: Certain members of the inhibitor of apoptosis (IAP) family, including BIRC6, regulate cell death by inhibiting caspases, while SMAC antagonizes IAPs and promotes cell death. BIRC6 directly inhibits caspase-3 and-7 and ubiquitinates caspase-3,-7, and-9, working with noncanonical E1, UBA6. SMAC suppresses both mechanisms by competitively displacing caspases.
Article
Oncology
Magali Espinosa, Floria Lizarraga, Karla Vazquez-Santillan, Alfredo Hidalgo-Miranda, Patricia Pina-Sanchez, Javier Torres, Roman A. Garcia-Ramirez, Vilma Maldonado, Jorge Melendez-Zajgla, Gisela Ceballos-Cancino
Summary: In breast cancer patients, higher levels of Smac/DIABLO gene and protein are positively associated with estrogen receptor (ER) positive samples, though the exact regulation mechanism remains unknown. Experimental results demonstrate that Smac/DIABLO is not regulated by ER and does not participate in the modulation of ER expression.
Article
Biochemistry & Molecular Biology
Giacomo Quilici, Andrea Berardi, Chantal Fabris, Michela Ghitti, Marco Punta, Louise J. Gourlay, Martino Bolognesi, Giovanna Musco
Summary: The SYLF domain of the BPSL1445 protein, a seroreactive antigen and diagnostic marker of Burkholderia pseudomallei, shows a beta-barrel core structure with flexible loops and helices. The study reveals weak interactions with phosphoinositides, supporting lipid binding abilities in prokaryotes and suggesting a common ancestry with bacterial EipA domains.
ACS CHEMICAL BIOLOGY
(2022)
Review
Oncology
Maria Teresa Majorini, Mario Paolo Colombo, Daniele Lecis
Summary: This article reviews the role of mast cells in cancer, with a particular focus on breast cancer. Mast cells have an impact on the features and aggressiveness of cancer cells, but the eventual effect of mast cell infiltration is still controversial.
Article
Immunology
Flavio Di Pisa, Stefano De Benedetti, Enrico Mario Alessandro Fassi, Mauro Bombaci, Renata Grifantini, Angelo Musico, Roberto Frigerio, Angela Pontillo, Cinzia Rigo, Sandra Abelli, Romualdo Grande, Nadia Zanchetta, Davide Mileto, Alessandro Mancon, Alberto Rizzo, Alessandro Gori, Marina Cretich, Giorgio Colombo, Martino Bolognesi, Louise Jane Gourlay
Summary: Chagas disease is a vector-borne parasitic disease caused by the protozoan parasite Trypanosoma cruzi. It is affecting millions of people worldwide and is emerging in non-endemic regions due to climate change and increased immigration. This study focuses on developing new serodiagnostic molecules for early screening of the disease. A recombinant surface membrane antigen from T. cruzi was produced and its ability to detect plasma antibodies from infected patients was confirmed. The crystal structure of the antigen was also determined to guide the design of epitopes for CD diagnosis.
Article
Oncology
Emanuela Fina, Loredana Cleris, Matteo Dugo, Mara Lecchi, Chiara Maura Ciniselli, Daniele Lecis, Giulia Valeria Bianchi, Paolo Verderio, Maria Grazia Daidone, Vera Cappelletti
Summary: The study highlights the importance of understanding the hematogenous phase of breast cancer metastasis and identifies genes associated with metastasis through transcriptome analysis of circulating tumor cells. These findings provide valuable information on disease progression and potential therapeutic targets.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Virology
Rafaela Milan Bonotto, Francesco Boni, Mario Milani, Antonio Chaves-Sanjuan, Silvia Franze, Francesca Selmin, Tommaso Felicetti, Martino Bolognesi, Soultana Konstantinidou, Monica Poggianella, Chantal L. Marquez, Federica Dattola, Monica Zoppe, Giuseppe Manfroni, Eloise Mastrangelo, Alessandro Marcello
Summary: Pyridobenzothiazolone derivatives are a promising class of antiviral compounds with broad-spectrum activity. The HeE1-17Y derivative has been shown to be potent against flaviviruses and West Nile virus. It interacts directly with viral particles, compromising their infectivity. This compound alters the viral membrane and exhibits virucidal activity.
Article
Oncology
Milena Perrone, Claudia Chiodoni, Mara Lecchi, Laura Botti, Barbara Bassani, Annamaria Piva, Elena Jachetti, Matteo Milani, Daniele Lecis, Elda Tagliabue, Paolo Verderio, Sabina Sangaletti, Mario P. Colombo
Summary: Cancer can reprogram the bone marrow niche through the BM mesenchymal stem cells (MSC) and IL-1B/ATF3 signaling pathway, which promotes myeloid cell differentiation and tumor development. The expression of CD11b thorn population in the peripheral blood can be used as a potential biomarker for early diagnosis of breast cancer. This study suggests that targeting IL-1B or ATF3 may have therapeutic benefits for breast cancer patients.
Article
Biotechnology & Applied Microbiology
Ferdinando M. Milazzo, Antonio Chaves-Sanjuan, Olga Minenkova, Daniela Santapaola, Anna M. Anastasi, Gianfranco Battistuzzi, Caterina Chiapparino, Antonio Rosi, Emilio Merlo Pich, Claudio Albertoni, Emanuele Marra, Laura Luberto, Cecile Viollet, Luigi G. Spagnoli, Anna Riccio, Antonio Rossi, Gabriella Santoro, Federico Ballabio, Cristina Paissoni, Carlo Camilloni, Martino Bolognesi, Rita De Santis
Summary: A human single-chain antibody called scFv76 can neutralize different variants of SARS-CoV-2 and demonstrate therapeutic efficacy in a mouse model of COVID-19, reducing lung inflammation and endothelial damage.
Article
Biochemistry & Molecular Biology
Michele Fiore, Michele Mosconi, Francesco Boni, Alice Parodi, Annalisa Salis, Bruno Tasso, Eloise Mastrangelo, Enrico Millo, Federica Cossu
Summary: Inhibitor of Apoptosis Proteins (IAPs) are important targets for cancer therapy, but their activity regulation within the NF-kappa B pathway can cause chemoresistance. The molecule FC2 was found to modulate the NF-kappa B pathway in MDA-MB-231 adenocarcinoma cancer cells. FC2 combined with Smac-mimetics or TNF showed improved cytotoxic effects. The study also proposed 22 derivatives of FC2 with modified structures and evaluated their efficacy/potency in cells and binding affinity to the target proteins.
Article
Multidisciplinary Sciences
Javier Garcia-Pardo, Andrea Bartolome-Nafria, Antonio Chaves-Sanjuan, Marcos Gil-Garcia, Cristina Visentin, Martino Bolognesi, Stefano Ricagno, Salvador Ventura
Summary: The study reports the cryo-electron microscopy structure of hnRNPDL-2 fibrils, revealing its stable, non-toxic, and nucleic acid-binding properties. The structure provides insights into the mechanism of hnRNPDL-2 fibrillation and its association with LGMD D3. Additionally, the study highlights how alternative splicing controls the assembly of different hnRNPDL isoforms, generating functional diversity.
NATURE COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Martina Pedrini, Angelo Iannielli, Lorenzo Meneghelli, Daniele Passarella, Vania Broccoli, Pierfausto Seneci
Summary: The rational design of a small array of six Anle138b-centered PROTACs, 7a,b, 8a,b and 9a,b, targeting aSynuclein (aSyn) aggregates for binding, polyubiquitination by the E3 ligase Cereblon (CRBN), and proteasomal degradation, was used in the search for disease-modifying agents targeted against Parkinson's disease. Lenalidomide and thalidomide served as CRBN ligands and were coupled with amino- and azido Anle138b derivatives through flexible linkers and coupling reactions. Four Anle138b-PROTACs, 8a,b and 9a,b, were tested against aSyn aggregation in vitro using Thioflavin T (ThT) fluorescence assay and dopaminergic neurons derived from SNCA multiplications. The most promising inhibitor/degradation inducer of aSyn aggregation was identified as Anle138b-PROTAC 8a, which may have potential applications in synucleinopathies and cancer.
Article
Chemistry, Medicinal
Antonia I. Antoniou, Giulia Nordio, Maria Luisa Di Paolo, Eleonora Colombo, Beatrice Gaffuri, Laura Polito, Arianna Amenta, Pierfausto Seneci, Lisa Dalla Via, Dario Perdicchia, Daniele Passarella
Summary: 2-Hydroxyoleic acid (6, 2OHOA), a nontoxic antitumor drug, was used to form nanoparticles (NPs) in water and enhance cell penetration and drug release. The synthesized NP formulations showed antiproliferative activity against human tumor cell lines. The disulfide-containing linker in the nanoformulations promoted cellular effects.
Article
Biochemistry & Molecular Biology
Eleonora Colombo, Stefania Olla, Cristina Minnelli, Alessia Formato, Caterina Veroni, Silvia Corbisiero, Mattia Pericolo, Chiara Siguri, Giovanna Mobbili, Cristina Agresti, Pierfausto Seneci
Summary: In this study, twelve analogues of Edaravone (EDA) were synthesized to find potential remyelinating agents for multiple sclerosis treatment and identify their molecular targets. The compounds were evaluated using three primary assays, and a suitable EDA 1 prodrug for in vivo testing was selected.
Article
Biochemistry & Molecular Biology
Ida Freda, Cecile Exertier, Anna Barile, Antonio Chaves-Sanjuan, Mirella Vivoli Vega, Michail N. Isupov, Nicholas J. Harmer, Elena Gugole, Paolo Swuec, Martino Bolognesi, Anita Scipioni, Carmelinda Savino, Martino Luigi Di Salvo, Roberto Contestabile, Beatrice Vallone, Angela Tramonti, Linda Celeste Montemiglio
Summary: Specificity in protein-DNA recognition is determined by the structural and chemical features of the targeted DNA molecule. Using cryo-EM and crystallography, we elucidated the interactions driving the DNA recognition and binding by PdxR, a bacterial transcription factor. Our findings revealed the importance of electrostatic interactions and DNA bending in the allosteric regulation of DNA binding by PdxR. This study provides detailed insights into the structure and dynamics of the PdxR-DNA complex and the regulatory mechanisms of the MocR family of transcription factors.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Medicinal
Roberta Mancuso, Lorenzo Agostino Citterio, Simone Agostini, Ivana Marventano, Francesca La Rosa, Francesca Re, Pierfausto Seneci, Marina Saresella, Mario Clerici, Leonidas A. Phylactou
Summary: The anti-hyperglycemic drug glibenclamide and bi-functionalized liposomes are capable of inhibiting NLRP3 inflammasome activation and reducing the release of inflammatory cytokines. They also have the ability to modulate the expression of certain miRNAs. These findings suggest a potential new strategy for treating neurodegenerative diseases using glibenclamide and liposomes.
Article
Chemistry, Analytical
Silvia Picciolini, Francesca Roda, Alice Gualerzi, Valentina Mangolini, Luana Forleo, Aurora Mangolini, Silvia Sesana, Antonia Antoniou, Francesca Re, Pierfausto Seneci, Marzia Bedoni
Summary: This study presents the use of Surface Plasmon Resonance imaging (SPRi) for characterization of multifunctionalized liposomes designed for targeted Glioblastoma treatment, showing promising results in targeting brain and diseased areas.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
