Article
Cell Biology
Xiu Kui Gao, Zu Kang Sheng, Ye Hong Lu, Yu Ting Sun, Xi Sheng Rao, Lin Jing Shi, Xiao Xia Cong, Xiao Chen, Hao Bo Wu, Man Huang, Qiang Zheng, Jian-sheng Guo, Liang Jun Jiang, Li Ling Zheng, Yi Ting Zhou
Summary: In this study, it was found that VAPB protein on the endoplasmic reticulum (ER) interacts directly with IRS-1 protein, targeting it to the ER and maintaining its stability. This discovery reveals a new mechanism of stabilizing IRS-1 through VAPB.
Article
Environmental Sciences
Ting Zhang, Jie Lu, Ying Yao, Yanting Pang, Xiaomeng Ding, Meng Tang
Summary: This study aims to explore the importance of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress-induced autophagy in CdTe QDs toxicity, and the different cell outcomes in normal cells and cancer cells. The results showed that CdTe QDs induce ROS generation and prolonged ER stress in normal human liver cells (L02), leading to apoptosis. In contrast, CdTe QDs activate protective cellular autophagy in human liver cancer cells (HepG2 cells), preventing apoptosis.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Environmental
Xudan Liu, Yao Chen, Huanhuan Wang, Yuting Wei, Ye Yuan, Qianqian Zhou, Fang Fang, Sainan Shi, Xiaojing Jiang, Yinqiao Dong, Xin Li
Summary: Arsenic is the primary toxicant among hazardous environmental chemicals, implicated in neurotoxicity such as cognitive dysfunction, neurodevelopmental changes, and neurodegenerative diseases. It is believed that sustained pro-inflammatory response may trigger arsenic-induced neurotoxicity, potentially involving microglia activation and release of pro-inflammatory cytokines. Further research is needed to clarify the role of these cytokines in arsenic-induced neurotoxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Haohao Liu, Zhihui Tian, Yaxin Guo, Xiaohui Liu, Ya Ma, Xingde Du, Rui Wang, Shiyu Zhang, Linjia Shi, Hongxiang Guo, Huizhen Zhang
Summary: The study found that MC-LR can cause female reproductive toxicity by inducing apoptosis and follicular atresia in ovarian cells. The endoplasmic reticulum stress plays a crucial role in the apoptotic pathway induced by MC-LR, where Ddit3 regulates cell apoptosis and follicular atresia by modulating other genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Shanliang Zheng, Dong Zhao, Guixue Hou, Song Zhao, Wenxin Zhang, Xingwen Wang, Li Li, Liang Lin, Tie-Shan Tang, Ying Hu
Summary: The study demonstrates the interaction between TMCO1 and iASPP in colon cancer, affecting tumor growth and cell apoptosis by modulating Ca2+ homeostasis. Intervention in the iASPP-TMCO1 axis can effectively inhibit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Wenhan Wang, Tinghao Liu, Yuanyuan Liu, Lechu Yu, Xiaoqing Yan, Wenya Weng, Xuemian Lu, Chi Zhang
Summary: The study demonstrated that astaxanthin (AST) can attenuate ethanol-induced cardiac endoplasmic reticulum (ER) stress and subsequent apoptosis, alleviating chronic excessive ethanol consumption-induced cardiomyopathy.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Environmental Sciences
Min-Hua Wu, Su-Chun Hui, Yong-Syuan Chen, Hui-Ling Chiou, Ching-Yi Lin, Chien-Hsing Lee, Yi-Hsien Hsieh
Summary: The combination therapy of NCTD and PTX effectively inhibits cell viability and proliferation, induces G2/M phase arrest, cell death, ER stress, and downregulates SIRT7 expression in PCa cells. This treatment strategy may become a potential therapeutic approach against human PCa.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Jicang Wang, Lulu Ding, Ke Wang, Ruxue Huang, Wenjing Yu, Bingzhao Yan, Hongwei Wang, Cai Zhang, Zijun Yang, Zongping Liu
Summary: This study aimed to determine the protective effect of quercetin (Que) on cadmium (Cd)-induced hepatotoxicity. Through in vivo and in vitro experiments using rat models, the researchers found that Cd caused liver injury and cell apoptosis, and activated endoplasmic reticulum stress (ERS) signaling pathways. However, Que was able to improve hepatocyte vitality, reduce liver damage and oxidative stress caused by Cd.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Yiqi Wang, Lian Duan, Xing Zhang, Yang Jiao, Ying Liu, Lingling Dai, Hong Yan
Summary: The study demonstrated that chronic exposure to ACR can impair motor skills, muscle strength, and coordination in rats, as well as damage the morphology of the cerebellum, leading to increased apoptosis and initiation of autophagy.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Pharmacology & Pharmacy
Yazhou Guo, Chen Yang, Rong Guo, Ruijie Huang, Yongxia Su, Shuai Wang, Yezi Kong, Jianguo Wang, Chengjian Tan, Chonghui Mo, Chenchen Wu, Baoyu Zhao
Summary: The study revealed that MCT exhibits significant cytotoxicity on primary rat hepatocytes and induces ER stress, leading to apoptosis. Inhibiting ER stress or knocking down CHOP can partially alleviate cell apoptosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Toxicology
Sung Woo Lee, Bokyung Kim, Jung Bae Seong, Young-Ho Park, Hong Jun Lee, Dong-Seok Lee
Summary: In this study, the mechanisms of cadmium exposure-induced endoplasmic reticulum (ER) stress response and apoptosis in spermatocytes were examined. The most significant response to cadmium toxicity was observed in the activating transcription factor 6 (ATF6) pathway. Inhibition of protein expression could reduce apoptosis under stressful conditions. Furthermore, p50ATF6 was found to regulate p38 MAPK phosphorylation, and p-p38 mediated the activity of p50ATF6, suggesting that modulating the activity of p38 MAPK and p50ATF6 can be a potential strategy to treat infertility caused by cadmium exposure.
TOXICOLOGICAL SCIENCES
(2023)
Article
Environmental Sciences
Huiling Guo, Ruiming Hu, Gang Huang, Wenjing Pu, Xuesheng Chu, Chenghong Xing, Caiying Zhang
Summary: This study found that exposure to molybdenum and/or cadmium triggers ER stress-induced apoptosis via Th1 polarization in duck spleens, leading to increased expression of apoptosis-related genes and proteins, disruption of Th1/Th2 balance, histological abnormalities, and trace elements imbalance.
Article
Oncology
Xiaoping Song, Xiangting Xu, Jiali Lu, Xiaoyuan Chi, Yue Pang, Qingwei Li
Summary: The novel Lamprey immune protein (LIP) derived from Lampetra japonica efficiently kills lung cancer cells by inducing cell membrane decomposition and activating the ER stress pathway, leading to apoptosis. Moreover, injection of LIP significantly inhibits tumor growth in nude mice, suggesting its potential application in lung cancer treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yang Tian, Runzhu Fan, Zhao Yin, Yongping Huang, Dong Huang, Fangyu Yuan, Aiping Yin, Guihua Tang, Rong Pu, Sheng Yin
Summary: Two new coumaroyl or feruloyl oleananes, Glochidpurnoids A and B (1 and 2), were isolated from the stems and twigs of Glochidion puberum, along with 17 known triterpenoids (3-19). The structures of these compounds were determined using various spectroscopic techniques, chemical methods, and single crystal X-ray diffraction. Cytotoxicity screening against colorectal cancer cell line HCT-116 revealed that compounds 2, 3, 5, 6, 11, and 17 exhibited significant inhibitory activities (IC50: 0.80-2.99 μM), surpassing the positive control 5-fluorouracil (5-FU). Mechanistic study of compound 2, the most potent compound, demonstrated its ability to induce endoplasmic reticulum (ER) stress-mediated apoptosis and enhance the sensitivity of HCT-116 cells to 5-FU.
Article
Genetics & Heredity
Yongshi Liu, Xiaohua Liang, Hongpei Zhang, Jiajia Dong, Yan Zhang, Juan Wang, Chunmei Li, Xiangbing Xin, Yan Li
Summary: This study found associations between polymorphisms of EIF2AK3, HSPA5, and DDIT3 genes and the risk of lung cancer. The A allele of rs6750998 was shown to be a protective allele, while the minor alleles of rs867529, rs391957, and rs697221 were all risk alleles. Different genotypes were associated with an increased or decreased risk of lung cancer.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Jana Key, Antonia Maletzko, Aneesha Kohli, Suzana Gispert, Sylvia Torres-Odio, Ilka Wittig, Juliana Heidler, Clea Barcena, Carlos Lopez-Otin, Yuanjiu Lei, A. Phillip West, Christian Muench, Georg Auburger
Article
Oncology
Qilin Wang, Xiangguo Liu
CANCER CELL INTERNATIONAL
(2020)
Article
Multidisciplinary Sciences
Baoyu Zhao, Pengbiao Xu, Chesley M. Rowlett, Tao Jing, Omkar Shinde, Yuanjiu Lei, A. Phillip West, Wenshe Ray Liu, Pingwei Li
Article
Oncology
Shun Yao, Feifei Shi, Ning Mu, Xiaopeng Li, Guilin Ma, Yingying Wang, Xiaoyang Sun, Xiangguo Liu, Ling Su
Summary: AAMP, considered a pro-tumor protein, is upregulated in non-small cell lung carcinoma and promotes migration and invasion in NSCLC cells. It interacts with CDC42 to activate it, leading to the formation of cellular protrusions. AAMP enhances CDC42 activation by impairing the combination of ARHGAP1 and CDC42.
Article
Immunology
Sylvia Torres-Odio, Yuanjiu Lei, Suzana Gispert, Antonia Maletzko, Jana Key, Saeed S. Menissy, Ilka Wittig, Georg Auburger, A. Phillip West
Summary: CLPP deficiency results in abnormal immune system responses in mice, leading to increased resistance to RNA and DNA virus infections. The instability and packaging alterations of mitochondrial DNA caused by CLPP deficiency drive the IFN-I signaling pathway.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Xiaopeng Li, Guilin Ma, Wenjie Guo, Ning Mu, Yingying Wang, Xiangguo Liu, Ling Su
Summary: Hhex plays a significant role in regulating lung cancer cell migration by inhibiting the activation of RHOA/CDC42, thus decreasing the ability of lung cancer cells to migrate.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Cell Biology
Theresa C. Sutherland, Arthur Sefiani, Darijana Horvat, Taylor E. Huntington, Yuanjiu Lei, A. Phillip West, Cedric G. Geoffroy
Summary: The age of incidence of spinal cord injury is increasing, and the average age of people living with SCI is also rising. However, research on SCI has mainly focused on young animals, making it difficult to translate findings to clinical applications. Aging neurons show dysfunctional mitochondria, which may be associated with age-dependent reduction in neurite growth. This presents a challenge for the aging SCI population, as both normal aging and traumatic injury are linked to mitochondrial dysfunction.
Article
Biochemistry & Molecular Biology
Yuanjiu Lei, Jordyn J. VanPortfliet, Yi-Fan Chen, Joshua D. Bryant, Ying Li, Danielle Fails, Sylvia Torres-Odio, Katherine B. Ragan, Jingti Deng, Armaan Mohan, Bing Wang, Olivia N. Brahms, Shawn D. Yates, Michael Spencer, Carl W. Tong, Marcus W. Bosenberg, Laura Ciaccia West, Gerald S. Shadel, Timothy E. Shutt, Jason W. Upton, Pingwei Li, A. Phillip West
Summary: Mitochondrial DNA (mtDNA) is a powerful activator of the innate immune system, and its specific immunostimulatory properties and detection by cytosolic nucleic acid sensors are still poorly understood. Our study demonstrates that mitochondrial genome instability leads to the accumulation of Z-form DNA. Z-DNA Binding Protein 1 (ZBP1) stabilizes Z-form mtDNA and forms a cytosolic complex with cGAS, RIPK1, and RIPK3, resulting in sustained phosphorylation of STAT1 and signaling of type I interferon (IFN-I). Increased levels of Z-form mtDNA, ZBP1 expression, and IFN-I signaling are observed in cardiomyocytes exposed to Doxorubicin, a commonly used chemotherapeutic drug that causes cardiotoxicity. Remarkably, mice lacking ZBP1 or IFN-I signaling are protected from Doxorubicin-induced cardiotoxicity. Our findings highlight ZBP1 as a potential therapeutic target in heart failure and other disorders where mtDNA stress contributes to interferon-related pathology.
Article
Cell Biology
Limei Xu, Feifei Shi, Yingdi Wu, Shun Yao, Yingying Wang, Xukai Jiang, Ling Su, Xiangguo Liu
Summary: Experimental research has found that GSDME regulates the stability of EGFR, with GSDME-FL increasing EGFR stability and GSDME N-terminal fragment promoting EGFR degradation. These results reveal the link between GSDME and EGFR stability and provide new insights into the development of non-small cell lung cancer.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Iman Al Khatib, Jingti Deng, Yuanjiu Lei, Sylvia Torres-Odio, Gladys R. Rojas, Laura E. Newman, Brian K. Chung, Andrew Symes, Hongliang Zhang, Shar-yin N. Huang, Yves Pommier, Aneal Khan, Gerald S. Shadel, Andrew Phillip West, William T. Gibson, Timothy E. Shutt
Summary: The discovery of the P193L variant in TOP1MT, which affects its function, in a family with multiple autoimmune diseases, including SLE, highlights the role of TOP1MT in regulating the release of mitochondrial DNA and activating the cGAS-STING pathway.
HUMAN MOLECULAR GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Yu Li, Ji Geng, Suman Rimal, Haochuan Wang, Xiangguo Liu, Bingwei Lu, Shuangxi Li
Summary: This study demonstrates that mTORC2/AKT signaling is activated by APP, TDP-43 and FUS, and that mTORC2/AKT and its downstream target valosin-containing protein mediate the effect of these proteins on the quality control of C9-ALS/FTD-associated poly(GR) translation. The findings also suggest that mTORC2/AKT signaling and GCN2/eIF2 alpha integrated stress response are common signaling pathways underlying ALS/FTD pathogenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Emily B. Warren, Eliza M. Gordon-Lipkin, Foo Cheung, Jinguo Chen, Amrita Mukherjee, Richard Apps, John S. Tsang, Jillian Jetmore, Melissa L. Schlein, Shannon Kruk, Yuanjiu Lei, A. Phillip West, Peter J. McGuire
Summary: Transcriptional differences in immune dysregulation were found between mitochondrial disease (MtD) patients and healthy controls, characterized by activation of inflammatory signaling pathways and enrichment of antiviral response genes. This suggests a link between mitochondrial dysfunction and inflammation in MtD.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Yingying Wang, Minli Wei, Min Su, Zhiyuan Du, Jiaxi Dong, Yu Zhang, Yingdi Wu, Xiaopeng Li, Ling Su, Xiangguo Liu
Summary: DIRAS3, a candidate tumor suppressor protein, inhibits the migration of NSCLC cells by promoting the polyubiquitination and degradation of RAC1 through the interaction with RNF19B. The DIRAS3-RNF19B-RAC1 axis is associated with the malignant progression of NSCLC.
Article
Cell Biology
Ning Mu, Yu Wang, Xiaopeng Li, Zhiyuan Du, Yingdi Wu, Min Su, Yingying Wang, Xiaoyang Sun, Ling Su, Xiangguo Liu
Summary: The study reveals that crotonylated BEX2 inhibits chemotherapy-induced apoptosis in lung cancer cells by enhancing mitophagy. BEX2 promotes mitophagy by facilitating the interaction between NDP52 and LC3B, and the crotonylation of BEX2 at K59 is crucial for mitophagy. Overexpression of BEX2 inhibits chemotherapy-induced apoptosis, and clinical observations show that BEX2 overexpression is associated with poor prognosis in lung adenocarcinoma.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Yuanjiu Lei, Camila Guerra Martinez, Sylvia Torres-Odio, Samantha L. Bell, Christine E. Birdwell, Joshua D. Bryant, Carl W. Tong, Robert O. Watson, Laura Ciaccia West, A. Phillip West
Summary: In the mutator mouse model with mitochondrial DNA instability, aberrant activation of the IFN-I immune axis leads to immunometabolic dysfunction, shortened lifespan, and accelerated aging. Elevated IFN-I signaling suppresses activation of NRF2, increasing oxidative stress and proinflammatory responses, resulting in metabolic dysfunction.