期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 368, 期 2, 页码 343-349出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.01.062
关键词
eicosapentaenoic acid; tumor necrosis factor-alpha; matrix metalloproteinase-9; NF-kappa B
Eicosapentaenoic acid (EPA) is an omega-3 (omega-3) polyunsaturated fatty acid (PUFA), which has anti-inflammatory and anti-cancer properties. Some reports have demonstrated that EPA inhibits NF-kappa B activation induced by tumor necrosis factor (TNF)-alpha or lipopolysaccharide (LPS) in various cells. However, its detailed mode of action is unclear. In this report, we investigated whether EPA inhibits the expression of TNF-alpha-induced matrix metalloproteinases (MMP)-9 in human immortalized keratinocytes (HaCaT). TNF-alpha induced MMP-9 expression by NF-kappa B-dependent pathway. Pretreatment of EPA inhibited TNF-alpha-induced MMP-9 expression and p65 phosphorylation. However, EPA could not affect I kappa B-alpha phosphorylation, nuclear translocation of p65, and DNA binding activity of NF-kappa B. EPA inhibited TNF-alpha-induced p65 phosphorylation through p38 and Akt inhibition and this inhibition was IKK alpha-dependent event. Taken together, we demonstrate that EPA inhibits TNF-alpha-induced MMP-9 expression through inhibition of p38 and Akt activation. (c) 2008 Elsevier Inc. All rights reserved.
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