Article
Biochemistry & Molecular Biology
Ashley K. Putman, G. Andres Contreras
Summary: Dysregulated inflammation and oxidative stress play significant roles in various diseases. Macrophages are crucial in immune responses and regulate inflammation by exhibiting different phenotypes. IsoPs, as biomarkers of oxidative stress, can promote an anti-inflammatory macrophage phenotype during inflammation, contributing to disease prevention and mitigation.
Article
Integrative & Complementary Medicine
Tu Yan-jie, Tan Bo, Jiang Lei, Wu Zhong-hua, Yu Hong-ji, Li Xiao-qian, Yang Ai-dong
Summary: Emodin can inhibit inflammation of LPS-induced mice RAW264.7 macrophages by activating autophagy. Emodin reduces the expression of NF-kappa B, P62, and p-mTOR, while increasing I kappa B alpha expression, LC3B II/I ratio, and LC3B fluorescence intensity. The enhanced autophagic effects of emodin, such as increased LC3B II/I ratio and decreased P62 expression, are suppressed by the autophagy inhibitor 3-MA.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2021)
Article
Biochemical Research Methods
Robina Antony, Jijin Raveendran, Prabath Gopalakrishnan Biju
Summary: This study demonstrates the potent anti-inflammatory effects of the methanolic extract of Mollugo cerviana in an in vitro acute inflammation model. The extract suppresses the LPS-induced inflammatory response in mouse macrophage cells, indicating its potential as a therapeutic agent for inflammatory conditions.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2022)
Article
Biochemistry & Molecular Biology
Dong-Geun Han, Min-Jun Bae, Bong-Jeon An
Summary: This study verified the application of Mucuna pruriens extracts as a natural-based raw material with anti-inflammatory function. The extracts exhibited nitric oxide inhibition activity, with the extracts obtained using hot water and 70% ethanol showing the best activity. Western blot analysis confirmed the inhibitory effects of these extracts on inflammation-related proteins. Furthermore, high levels of L-DOPA were detected in the extracts. Therefore, Mucuna pruriens extracts have the potential to be used as anti-inflammatory materials.
Article
Immunology
Hakseong Lim, Jun-Young Park, Fukushi Abekura, Hyunju Choi, Hee-Do Kim, Junji Magae, Young-Chae Chang, Young-Choon Lee, Cheorl-Ho Kim
Summary: The study demonstrates that MAC alleviates inflammatory responses induced by lipopolysaccharide in murine macrophages by modulating the MAPK signaling pathway and downregulating NF-kappa B-related genes. MAC treatment decreases the expression of pro-inflammatory indicators and cytokines, potentially offering therapeutic benefits in reducing prolonged inflammation-related damage. The structural differences between ASC and its derivatives, as well as their impact on intracellular signaling, are also discussed.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Plant Sciences
Adhimoolam Karthikeyan, Hun Hwan Kim, Vetrivel Preethi, Mohammad Moniruzzaman, Ki Ho Lee, Senthil Kalaiselvi, Gon Sup Kim, Taesun Min
Summary: Citrus unshiu peel contains various flavonoids, with a high concentration of naringin. Treatment with flavonoids can reduce inflammation and oxidative damage in LPS-stimulated RAW 264.7 macrophages, showing potential for treating inflammation-related diseases.
Article
Immunology
Meenakshi Bhardwaj, Veeresh Kumar Sali, R. Malarvizhi, Sugumar Mani, T. K. Padmavathy, Hannah Rachel Vasanthi
Summary: This study identifies a fatty acid methyl ester, methyl 6,12-dimethyltridecanoate (ET), isolated from the brown algae Turbinaria ornata, which possesses anti-inflammatory, antioxidant, and immunomodulatory potential. In vitro and in silico studies demonstrate the significant anti-inflammatory effects of ET, including the inhibition of inflammatory markers and the promotion of antioxidant enzymes. The findings establish a molecular basis for the anti-inflammatory properties of ET and its potential as a therapeutic agent.
INFLAMMOPHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Seung-Hyuk Choi, So-Yeon Kim, Kyeong-Min Kim, Tamanna Jahan Mony, Ho Jung Bae, Min Seok Kim, Chan Ho Lee, Sun-Eun Choi, Sang Ho Lee, Se Jin Park
Summary: The interest in bioconversion through fermentation of sprouts produced in smart farms is increasing due to their potential health benefits. Codonopsis lanceolata (CL) is reported to alleviate inflammatory conditions, but much research is still needed to determine which types and parts of CL are most effective. This study investigated the anti-inflammatory effects of a fermented extract of CL sprouts' aerial part (F-CSA) against LPS-stimulated RAW 264.7 macrophages and mice.
Article
Pharmacology & Pharmacy
Edward E. Putnins, Verena Goebeler, Mahyar Ostadkarampour
Summary: RG0216 significantly decreased LPS-induced IL-6 and IL-1 beta gene and protein expression in an epithelial cell culture model, with similar effectiveness to deprenyl. This reduction occurred downstream of the cAMP-PKA/EPAC signaling cascade, providing a novel mechanism by which MAO-B selective inhibitors regulate LPS-induced cytokine expression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Integrative & Complementary Medicine
Su-Hyeon Cho, Song Rae Kim, Myeong Seon Jeong, Miri Choi, SeonJu Park, Kil-Nam Kim
Summary: The experiment demonstrated that Brassica napus L. hydrosols exhibit significant anti-inflammatory activity in LPS-stimulated RAW 264.7 cells by reducing the generation of nitric oxide and prostaglandin E-2, inhibiting the expression of inducible nitric oxide synthase and cyclooxygenase-2, and blocking the NF-kappa B signaling pathways.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2021)
Article
Plant Sciences
Hamsalakshmi, Suresh Joghee, Sreeram P. Kalarikkal, Gopinath M. Sundaram, T. Durai Ananda Kumar, Saravana Babu Chidambaram
Summary: The study demonstrated the anti-inflammatory effects of extracts from the aerial parts of Trichodesma indicum, with fraction FE showing the strongest ability to reduce inflammatory cytokines without cytotoxicity. The major compound in FE, 1,2-benzenedicarboxylic acid diisooctyl ester, exhibited good inhibition against key inflammatory targets.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Seungmin Yu, Hyejeong Park, Wooki Kim
Summary: Studies have shown that trehalose has beneficial effects on metabolic syndromes, hyperlipidemia, and autophagy, but its mechanisms of action are still not fully understood. This study aimed to investigate the effects of trehalose on immunological phenotypes, energy metabolism, and LPS-induced macrophage mitochondrial functioning. The results suggest that trehalose reduces inflammatory mediators in LPS-induced macrophages and suppresses inflammatory cytokines and mediators via energy metabolism reprogramming.
Article
Multidisciplinary Sciences
A. M. Kosyreva, P. A. Vishnyakova, I. S. Tsvetkov, V. V. Kiseleva, D. Sh. Dzhalilova, E. A. Miroshnichenko, A. V. Lokhonina, O. V. Makarova, T. H. Fatkhudinov
Summary: This study aimed to evaluate the influence of infusions of M2-polarized RAW 264.7 murine macrophage cell line on the development of LPS-induced ARDS. The results indicate that the infusions promote relocation of lymphocytes from immune organs to the lungs and activate an anti-inflammatory response. However, treatment with M2-polarized macrophages did not change the neutrophil numbers in the lungs.
Article
Biochemistry & Molecular Biology
Bibiana Silva, Thiago Caon, Eduarda Talita Bramorski Mohr, Fabiola Carina Biluca, Luciano Valdomiro Gonzaga, Roseane Fett, Eduardo Monguilhott Dalmarco, Ana Carolina Oliveira Costa
Summary: This study investigated the anti-inflammatory potential of bracatinga honeydew honey and found that geographical location affects its effectiveness. Honey samples collected from areas with greater vegetation diversity and density were more efficient at inhibiting inflammatory markers.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Y. J. Choi, I. Y. Bae
Summary: White-spotted flower chafer (Protaetia brevitarsis) is an edible insect used in traditional Oriental medicine. The study investigated the anti-inflammatory effects of P. brevitarsis larvae ethanol extract (PBLE) on lipopolysaccharide-stimulated macrophages and identified its molecular mechanisms. PBLE effectively inhibited the synthesis of nitric oxide and pro-inflammatory cytokines, potentially through selective inhibition of NF-kappa B, ERK, and p38 MAPK signaling pathways. Further research is needed to confirm and understand the anti-inflammatory actions of PBLE for potential therapeutic applications.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)