☆ 4.6 Article

Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2008)

期刊

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

卷 377, 期 2, 页码 341-345

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE

DOI: 10.1016/j.bbrc.2008.09.150

关键词

Sulforaphane (SFN); DNA double strand breaks (DSBs); Homologous recombination repair (HRR); Non-homologous end joining (NHEJ)

类别

Biochemistry & Molecular Biology Biophysics

资金

Japan Society for the Promotion of Science (JSPS) [16209036]
Grants-in-Aid for Scientific Research [16209036] Funding Source: KAKEN

向作者/读者索取更多资源

Protocol

Reagent

摘要

Cytotoxicity and DNA double strand breaks (DSBs) were studied in HeLa cells treated With Sulforaphane (SFN), a well-known chemo-preventive agent. Cell survival was impaired by SFN in a concentration and treatment time-dependent manner. Both constant field gel electrophoresis (CFGE) and gamma-H2AX assay unambiguously indicated formation of DSBs by SFN, reflecting the cell Survival data. These DSBs were predominantly processed by homologous recombination repair (HRR), judging from the SFN concentration-dependent manner of Rad51 foci formation. On the other hand, the phosphorylation of DNA-PKcs, a key non-homologous end joining (NHEJ) protein, was not observed by SFN treatment, suggesting that NHEJ may not be involved in DSBs induced by this chemical. G2/M arrest by SFN, a typical response for cells exposed to ionizing radiation was also observed. Our new data indicate the clear induction of DSBs by SFN and a useful anti-tumour aspect of SFN through the induction of DNA DSBs. (C) 2008 Elsevier Inc. All rights reserved.

作者

我是这篇论文的作者

点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6

评分不足

次要评分

新颖性

-

重要性

-

科学严谨性

-

评价这篇论文

推荐

Article Multidisciplinary Sciences

Gene expression of non-homologous end-joining pathways in the prognosis of ovarian cancer

Ethan S. Lavi, Z. Ping Lin, Elena S. Ratner

Summary: This study investigated the effects of non-homologous end-joining (NHEJ) genes on clinical outcomes of ovarian cancer patients, and identified several most important genes for predicting the prognosis of ovarian cancer patients.

ISCIENCE (2023)

添加到收藏夹

Review Biochemistry & Molecular Biology

Role of deubiquitinating enzymes in DNA double-strand break repair

Yunhui Li, Jian Yuan

Summary: DNA is the hereditary material in humans and most other organisms, crucial for accurate transmission of genetic information. Damage to DNA, including DSBs, can lead to gene mutations, genome instability, and even tumorigenesis if repair mechanisms are defective. Protein deubiquitination is essential in DNA DSB repair, and understanding the molecular mechanisms of DUB regulation can provide insights for combatting human diseases and developing new therapeutic approaches.

JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B (2021)

添加到收藏夹

Article Genetics & Heredity

Homologous Recombination and Repair Functions Required for Mutagenicity during Yeast Meiosis

Liat Morciano, Renana M. Elgrabli, Drora Zenvirth, Ayelet Arbel-Eden

Summary: Several events occur during meiosis to reshape the genome and transfer it to the next generation. The occurrence of new meiotic mutations is closely linked to homologous recombination and depends on Spo11-induced DNA breaks. Through studying the timing of mutation and recombination events in cells deficient in DNA HR-repair genes, we found that Rad54 and Tid1 play different roles in meiotic mutation occurrence. We also observed that single-stranded DNA may be a potential source for mutagenicity during meiosis. Overall, we propose that de novo mutations during meiosis contribute to the diversification of the genome.

GENES (2023)

添加到收藏夹

Review Cell Biology

The Interplay between the Cellular Response to DNA Double-Strand Breaks and Estrogen

Lia Yedidia-Aryeh, Michal Goldberg

Summary: Cancer development is often associated with impaired DNA repair and DNA damage signaling pathways. Estrogen has a regulatory role in the repair and cellular response to DNA double-strand breaks. There is a complex interplay between the cellular DNA damage response and the actions of estrogen, which may contribute to the development of estrogen-dependent cancers.

CELLS (2022)

添加到收藏夹

Review Genetics & Heredity

Immediate-Early, Early, and Late Responses to DNA Double Stranded Breaks

Shaylee R. Kieffer, Noel F. Lowndes

Summary: Loss or rearrangement of genetic information can occur due to incorrect responses to DNA double strand breaks (DSBs). Cellular responses to DSBs involve coordinated events to detect and appropriately respond to the damage, preserving genomic integrity. This article discusses the complex regulation of the immediate-early, early, and late responses to DSBs prior to repair.

FRONTIERS IN GENETICS (2022)

添加到收藏夹

Article Genetics & Heredity

Concatenation of Transgenic DNA: Random or Orchestrated?

Alexander Smirnov, Nariman Battulin

Summary: The generation of transgenic organisms through pronuclear microinjection has become a routine procedure, but the recombination between transgene molecules immediately after injection is still not well understood. Injected molecules often form head-to-tail tandem repeats called concatemers. This review focuses on possible concatenation mechanisms, as well as features of concatemers such as palindromic junctions and repeat-induced gene silencing (RIGS), and speculates on how DNA repair pathways cooperate to create a multicopy concatenated insert.

GENES (2021)

添加到收藏夹

Article Plant Sciences

Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering

Fabienne Gehrke, Angelina Schindele, Holger Puchta

Summary: Heritable plant chromosome engineering can be achieved in somatic cells using CRISPR/Cas to induce nonhomologous double-strand break repair pathways. This technology allows for large-scale restructuring of plant chromosomes, including duplications, inversions, and translocations. The use of nonhomologous end joining pathways in somatic cells facilitates efficient chromosomal rearrangements. This breakthrough has the potential to revolutionize plant breeding.

PLANT PHYSIOLOGY (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

A pathway for error-free non-homologous end joining of resected meiotic double-strand breaks

Talia Hatkevich, Danny E. Miller, Carolyn A. Turcotte, Margaret C. Miller, Jeff Sekelsky

Summary: A novel backup repair mechanism has been identified in fruit fly meiosis, where DSB repair is initiated by homologous recombination but completed by nonhomologous end joining in the absence of the homologous chromosome. This precise repair process is proposed to be conserved and used to overcome challenges in normal meiosis.

NUCLEIC ACIDS RESEARCH (2021)

添加到收藏夹

Article Cell Biology

MicroRNA-145 Impairs Classical Non-Homologous End-Joining in Response to Ionizing Radiation-Induced DNA Double-Strand Breaks via Targeting DNA-PKcs

Muddenahalli Srinivasa Sudhanva, Gurusamy Hariharasudhan, Semo Jun, Gwanwoo Seo, Radhakrishnan Kamalakannan, Hyun Hee Kim, Jung-Hee Lee

Summary: This study demonstrates that miR-145 suppresses DNA-PKcs expression, affecting DNA double-strand break repair and rendering cells sensitive to ionizing radiation. Additionally, miR-145 inhibition is associated with increased Akt1 phosphorylation and upregulation of DNA-PKcs in cancer cells.

CELLS (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

DNA polymerase η promotes nonhomologous end joining upon etoposide exposure dependent on the scaffolding protein Kap1

Xiaolu Ma, Chen Wang, Bo Zhou, Zina Cheng, Zhiyong Mao, Tie-Shan Tang, Caixia Guo

Summary: This study demonstrated the important role of Pol eta in ETO-induced DSB repair, and identified Kap1 as a novel regulator. Kap1 regulates the function of Pol eta in DSB repair by facilitating Rad18 recruitment and stabilizing the Rad18-Pol eta association.

JOURNAL OF BIOLOGICAL CHEMISTRY (2022)

添加到收藏夹

Review Biochemistry & Molecular Biology

New Facets of DNA Double Strand Break Repair: Radiation Dose as Key Determinant of HR versus c-NHEJ Engagement

Emil Mladenov, Veronika Mladenova, Martin Stuschke, George Iliakis

Summary: Radiation therapy is an essential part of cancer management, utilizing different modalities of ionizing radiation to mitigate cancer progression. This article reviews the molecular mechanisms underlying the repair pathways involved in DNA damage caused by radiation therapy. It discusses factors and processes that influence the choice of repair pathways throughout the cell cycle and highlights the preference for homologous recombination at low radiation doses. The article also explores the molecular basis of transitions from high fidelity to error-prone repair pathways and analyzes the coordination and consequences of this transition on cell viability and genomic stability. Lastly, it discusses how these advances can contribute to the development of improved cancer treatment protocols in radiation therapy.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

添加到收藏夹

Article Biochemistry & Molecular Biology

RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination

Sijie Liu, Yu Hua, Jingna Wang, Lingyan Li, Junjie Yuan, Bo Zhang, Ziyang Wang, Jianguo Ji, Daochun Kong

Summary: Protection of 30 overhangs in DNA double-strand breaks (DSBs) repair is achieved through the transient formation of RNA-DNA hybrids, with RNA polymerase III (RNAPIII) responsible for synthesizing the RNA strand. CtIP and MRN nuclease activity are required for initiating RNAPIII-mediated RNA synthesis at DSBs. Reduced RNAPIII levels suppress homologous recombination (HR) and lead to genetic loss > 30 bp at DSBs.

CELL (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Mechanism of chromosome rearrangement arising from single-strand breaks

Palina Kot, Takaaki Yasuhara, Atsushi Shibata, Miyako Hirakawa, Yu Abe, Motohiro Yamauchi, Naoki Matsuda

Summary: Chromosome rearrangements, commonly found in human cancer, result from misrejoining of DNA double-strand breaks at different genomic regions. This study identified DNA single-strand breaks as a major source of double-strand breaks leading to chromosome rearrangements, repaired through homologous recombination. Downregulation of homologous recombination factors promoted the formation of radial chromosomes upon hydrogen peroxide treatment, highlighting the importance of these factors in suppressing gene fusion.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021)

添加到收藏夹

Review Biochemistry & Molecular Biology

Exploring the Origin and Physiological Significance of DNA Double Strand Breaks in the Developing Neuroretina

Noemi Alvarez-Lindo, Teresa Suarez, Enrique J. de la Rosa

Summary: Genetic mosaicism is an intriguing feature of the mammalian brain, similar to the immune system, that generates diversity in genetic information and cellular function. However, its origin and physiological significance are still poorly understood. Our studies in the developing retina shed light on the relationship between DNA rearrangements, neurogenesis, and early neuronal cell death, providing new insights into the mechanisms of DNA rearrangements in the developing brain.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

添加到收藏夹

Review Cell Biology

Mechanism, cellular functions and cancer roles of polymerase-theta-mediated DNA end joining

Dale A. Ramsden, Juan Carvajal-Garcia, Gaorav P. Gupta

Summary: DNA polymerase theta (Pol theta)-mediated end joining is a recently characterized DNA repair pathway that functions in various cellular contexts to repair DNA double-strand breaks that are not repaired by other pathways. Pol theta-mediated end joining both helps maintain the genome and causes genome instability, and is an emerging therapeutic target in cancer.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Resistance of Bacillus subtilis Spore DNA to Lethal Ionizing Radiation Damage Relies Primarily on Spore Core Components and DNA Repair, with Minor Effects of Oxygen Radical Detoxification

Ralf Moeller, Marina Raguse, Guenther Reitz, Ryuichi Okayasu, Zuofeng Li, Stuart Klein, Peter Setlow, Wayne L. Nicholson

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2014)

添加到收藏夹

Article Oncology

Radiosensitization effect of poly(ADP-ribose) polymerase inhibition in cells exposed to low and high liner energy transfer radiation

Takahisa Hirai, Hidenori Shirai, Hiroaki Fujimori, Ryuichi Okayasu, Keisuke Sasai, Mitsuko Masutani

CANCER SCIENCE (2012)

添加到收藏夹

Article Genetics & Heredity

The complexity of DNA double strand breaks is a critical factor enhancing end-resection

Hirohiko Yajima, Hiroshi Fujisawa, Nakako Izumi Nakajima, Hirokazu Hirakawa, Penelope A. Jeggo, Ryuichi Okayasu, Akira Fujimori

DNA REPAIR (2013)

添加到收藏夹

Review Oncology

Repair of DNA damage induced by accelerated heavy ionsuA mini review

Ryuichi Okayasu

INTERNATIONAL JOURNAL OF CANCER (2012)

添加到收藏夹

Article Medicine, Research & Experimental

Benzyl isothiocyanate sensitizes human pancreatic cancer cells to radiation by inducing apoptosis

Maki Ohara, Shinichi Kimura, Aya Tanaka, Ken Ohnishi, Ryuichi Okayasu, Nobuo Kubota

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2011)

添加到收藏夹

Article Medicine, Research & Experimental

Radiosensitization of human lung cancer cells by the novel purine-scaffold Hsp90 inhibitor, PU-H71

Tatsuya Segawa, Yoshihiro Fujii, Aya Tanaka, Shin-Ichi Bando, Ryuichi Okayasu, Ken Ohnishi, Nobuo Kubota

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2014)

添加到收藏夹

Article Biology

ASPM influences DNA double-strand break repair and represents a potential target for radiotherapy

Takamitsu A. Kato, Ryuichi Okayasu, Penny A. Jeggo, Akira Fujimori

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2011)

添加到收藏夹

Meeting Abstract Oncology

Radiosensitization Effect of PARP Inhibitor in Cells Exposed to Low and High LET Radiation

T. Hirai, H. Shirai, R. Okayasu, K. Sasai, M. Masutani

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS (2011)

添加到收藏夹

Article Microbiology

Role of the Nfo and ExoA Apurinic/Apyrimidinic Endonucleases in Radiation Resistance and Radiation-Induced Mutagenesis of Bacillus subtilis Spores

Ralf Moeller, Peter Setlow, Mario Pedraza-Reyes, Ryuichi Okayasu, Guenther Reitz, Wayne L. Nicholson

JOURNAL OF BACTERIOLOGY (2011)

添加到收藏夹

Article Biochemistry & Molecular Biology

Selective Enhancing Effect of Early Mitotic Inhibitor 1 (Emi1) Depletion on the Sensitivity of Doxorubicin or X-ray Treatment in Human Cancer Cells

Natsumi Shimizu, Nakako Izumi Nakajima, Takaaki Tsunematsu, Ikuko Ogawa, Hidehiko Kawai, Ryoichi Hirayama, Akira Fujimori, Akiko Yamada, Ryuichi Okayasu, Naozumi Ishimaru, Takashi Takata, Yasusei Kudo

JOURNAL OF BIOLOGICAL CHEMISTRY (2013)

添加到收藏夹

Article Biology

Relative biological effects of neutron mixed-beam irradiation for boron neutron capture therapy on cell survival and DNA double-strand breaks in cultured mammalian cells

Kakuji Okumura, Yuko Kinashi, Yoshihisa Kubota, Erika Kitajima, Ryuichi Okayasu, Koji Ono, Sentaro Takahashi

JOURNAL OF RADIATION RESEARCH (2013)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET)

Cuihua Liu, Tetsuya Kawata, Yoshiya Furusawa, Guangming Zhou, Kohei Inoue, Junichi Fukada, Ryuichi Kota, Kerry George, Francis Cucinotta, Ryuichi Okayasu

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS (2013)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Evaluation of SCCVII tumor cell survival in clamped and non-clamped solid tumors exposed to carbon-ion beams in comparison to X-rays

Ryoichi Hirayama, Akiko Uzawa, Nobuhiro Takase, Yoshitaka Matsumoto, Miho Noguchi, Kana Koda, Masakuni Ozaki, Kei Yamashita, Huizi Li, Yuki Kase, Naruhiro Matsufuji, Sachiko Koike, Shin-ichiro Masunaga, Koichi Ando, Ryuichi Okayasu, Yoshiya Furusawa

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS (2013)

添加到收藏夹

Article Multidisciplinary Sciences

Visualisation of γH2AX Foci Caused by Heavy Ion Particle Traversal; Distinction between Core Track versus Non-Track Damage

Nakako Izumi Nakajima, Holly Brunton, Ritsuko Watanabe, Amruta Shrikhande, Ryoichi Hirayama, Naruhiro Matsufuji, Akira Fujimori, Takeshi Murakami, Ryuichi Okayasu, Penny Jeggo, Atsushi Shibata

PLOS ONE (2013)

添加到收藏夹

Article Biology

OH Radicals from the Indirect Actions of X-Rays Induce Cell Lethality and Mediate the Majority of the Oxygen Enhancement Effect

Ryoichi Hirayama, Atsushi Ito, Miho Noguchi, Yoshitaka Matsumoto, Akiko Uzawa, Gen Kobashi, Ryuichi Okayasu, Yoshiya Furusawa

RADIATION RESEARCH (2013)

添加到收藏夹

Article Biochemistry & Molecular Biology

Protective effects of metformin in the pro-inflammatory cytokine induced intestinal organoids injury model

Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim

Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Ferritin-based fusion protein shows octameric deadlock state of self-assembly

V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov

Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

A nano-liposomal carrier containing p-coumaric acid for induction of targeted apoptosis on melanoma cells and kinetic modeling

Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi

Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

YRNA and tRNA fragments can differentiate benign from malignant canine mammary gland tumors

M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura

Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Hole behavior captured by analysis of instantaneous amplitude and phase of sarcosynced oscillations reveals wave characteristics of sarcomeric oscillations

Seine A. Shintani

Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Bupleurum exerts antiarrhythmic effects by inhibiting L-type calcium channels in mouse ventricular myocytes

Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma

Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Citrobacter koseri inhibits the growth of Staphylococcus epidermidis by suppressing iron utilization

Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita

Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Development of a Cre-recombination-based color-switching reporter system for cell fusion detection

Toshifumi Asano, Philipp Sasse, Takao Nakata

Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

TP53INP2 modulates the malignant progression of colorectal cancer by reducing the inactive form of β-catenin

Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu

Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

The four subunits of rabbit skeletal muscle lactate dehydrogenase do not exert their catalytic action additively

Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler

Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Interaction of gallium, indium, and vanadyl curcumin complexes with hen egg-white lysozyme (HEWL): Mechanistic aspects and evaluation of antiamyloidogenic activity

Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani

Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Progranulin-deficient macrophages cause cardiotoxicity under hypoxic conditions

Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa

Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Changes in myelinated nerve fibers induced by pulsed electrical stimulation: A microstructural perspective on the causes of electrical stimulation side effects

Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang

Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

Fatty acid elongation regulates mitochondrial 13-oxidation and cell viability in prostate cancer by controlling malonyl-CoA levels

Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler

Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

Article Biochemistry & Molecular Biology

The effects of noise exposure on hippocampal cognition in C57BL/6 mice via transcriptomics

Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si

Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.