Article
Ophthalmology
Weihao Chen, Georgios Tsissios, Anthony Sallese, Byran Smucker, Anh-Thu Nguyen, Junfan Chen, Hui Wang, Katia Del Rio-Tsonis
Summary: The study established optical coherence tomography (OCT) as an effective in vivo imaging modality for tracking and analyzing the entire process of newt lens regeneration. OCT was able to accurately identify key features of the regeneration process, such as changes in lens volume, and establish dynamic spatial relationships between tissues. The findings demonstrate OCT's potential for studying tissue plasticity and developing therapies for blinding disorders in higher vertebrates.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Cell Biology
Hannah E. Walters, Konstantin E. Troyanovskiy, Alwin M. Graf, Maximina H. Yun
Summary: Salamanders are able to regenerate their limbs by modulating cellular plasticity and inducing cellular senescence, which can enhance muscle dedifferentiation and regeneration. Senescent cells promote myotube cell cycle re-entry and reversal of muscle identity through secreted factors, with the FGF-ERK signalling axis playing a critical role. This study highlights the beneficial role of cellular senescence in complex structure regeneration.
Article
Multidisciplinary Sciences
Chihena H. Banda, Makoto Shiraishi, Kohei Mitsui, Yoshimoto Okada, Kanako Danno, Ryohei Ishiura, Kaho Maemura, Chikafumi Chiba, Akira Mizoguchi, Kyoko Imanaka-Yoshida, Kazuaki Maruyama, Mitsunaga Narushima
Summary: Through the use of various imaging techniques, researchers have discovered a novel intraosseous lymphatic network in newts, rather than the traditional lymphatic heart, which plays a crucial role in their lymphatic circulation and limb regeneration. This study reveals the fundamental anatomical differences between the immune systems of urodeles and mammals and provides a new model for investigating lymphatics and regeneration.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Ji-Wen Wu, Xu Zhang, Reiko Sekiya, Kiyoshi Aoyagi, Tao-Sheng Li
Summary: By analyzing histone modifications in newt tail tissue cells following amputation, it was found that amputation significantly increased stem cells and proliferating cells, and induced acetylation of several histone proteins. These results suggest that epigenetic regulation likely plays a role in newt tail regeneration following amputation.
STEM CELLS INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Tatsuyuki Ishii, Ikkei Takashimizu, Martin Miguel Casco-Robles, Yuji Taya, Shunsuke Yuzuriha, Fubito Toyama, Fumiaki Maruo, Kazuo Kishi, Chikafumi Chiba
Summary: The study on adult newts reveals that their skin can achieve scarless regeneration through unique processes, making them an ideal model for research on skin wound healing and regeneration in humans.
Article
Immunology
Andrew D. Jerome, Jeffrey R. Atkinson, Arnetta L. Moffatt, Jesse A. Sepeda, Benjamin M. Segal, Andrew R. Sas
Summary: Recent studies have shown that neutrophils are more diverse than previously thought, with unique subsets identified in various conditions such as bacterial and parasitic infections, cancer, and tissue injury and repair. The characteristics of infiltrating neutrophils depend on the nature of the stimulus, the stage of inflammation, and the tissue microenvironment. This study used advanced techniques to analyze neutrophil populations following injection of a fungal cell wall extract, revealing distinct gene and protein expressions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martin Miguel Casco-Robles, Kayo Yasuda, Kensuke Yahata, Fumiaki Maruo, Chikafumi Chiba
Summary: In this study, skin manipulation experiments were conducted on adult newts, and it was found that regardless of how the skin was manipulated, the newts were able to regenerate limbs with a normal axial pattern. The results suggest that the tissue under the skin, rather than the skin itself, is crucial for normal axial pattern formation in adult newt limb regeneration.
Article
Multidisciplinary Sciences
Abijeet Singh Mehta, Prajakta Deshpande, Anuradha Venkatakrishnan Chimata, Panagiotis A. Tsonis, Amit Singh
Summary: The study demonstrates that unique newt genes can restore missing photoreceptor cells in the Drosophila eye model by regulating cell proliferation and blocking cell death, and acting on the conserved Wingless (Wg)/Wnt signaling pathway. These newt genes show powerful repair capability in the Drosophila eye model.
Article
Developmental Biology
Takashi Takeuchi, Haruka Matsubara, Fumina Minamitani, Yukio Satoh, Sayo Tozawa, Tomoki Moriyama, Kohei Maruyama, Ken-ichi T. Suzuki, Shuji Shigenobu, Takeshi Inoue, Koji Tamura, Kiyokazu Agata, Toshinori Hayashi
Summary: This study generated mutants of Hox13 genes using the CRISPR/Cas9 system in newts and found that Hox13 genes are essential for digit formation in both development and regeneration. The predominant role of Hoxa13 in newt limbs, unlike in mouse limbs, suggests a potential contribution of Hoxa13 in the transition from fins to limbs. These findings demonstrate the conservation of Hox13 gene function in limb development and regeneration across taxa.
Article
Biotechnology & Applied Microbiology
Sarena Banu, Namami Gaur, Sowmya Nair, Tanuja Ravikrishnan, Shahida Khan, Sandhya Mani, Swarna Bharathi, Komal Mandal, Sowmya Vuppaladadium, Rowmika Ravi, Mir Quoseena, Mohammed M. Idris
Summary: This study utilized differential transcriptomics and proteomics analysis to investigate the role of various genes/proteins involved in the regeneration of zebrafish caudal fin tissue. The findings provide detailed insights into the expression of genes/proteins associated with the epimorphic regeneration process.
Article
Biochemistry & Molecular Biology
Lorena Carmona-Rodriguez, Aaron S. Gajadhar, Irene Blazquez-Garcia, Laura Guerrero, Manuel A. Fernandez-Rojo, Iker Uriarte, Maricruz Mamani-Huanca, Angeles Lopez-Gonzalvez, Sergio Ciordia, Antonio Ramos, Jose Ignacio Herrero, Maite G. Fernandez-Barrena, Maria Arechederra, Carmen Berasain, Jorge Quiroga, Bruno Sangro, Josepmaria Argemi, Fernando Pardo, Fernando Rotellar, Daniel Lopez, Coral Barbas, Matias A. Avila, Fernando J. Corrales
Summary: The liver is capable of regenerating itself after injury or partial hepatectomy, representing a therapeutic opportunity for liver disease patients. However, the causes of failed liver regeneration are still poorly understood. In this study, the regulation of liver proteome and phosphoproteome after partial hepatectomy was investigated in mice, providing detailed mechanisms of the early phase of liver regeneration. Additionally, the dynamic changes of serum proteome and metabolome in healthy liver transplant donors were analyzed. The results provide insights into the cellular processes orchestrating liver regeneration and may be useful for monitoring the regenerative process and preventing complications associated with liver resection.
Article
Engineering, Biomedical
Renwang Sheng, Jialin Chen, Hongmei Wang, Yifan Luo, Jia Liu, Zhixuan Chen, Qingyun Mo, Jiayu Chi, Chen Ling, Xin Tan, Qingqiang Yao, Wei Zhang
Summary: In this study, a functionalized SF-MMT nanocomposite hydrogel with dual-lineage bioactivity is developed for osteochondral regeneration, showing potential in the field of osteochondral tissue engineering.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Genetics & Heredity
Gang Ye, Yalong Feng, Zhaoxiang Mi, Du Wang, Shuai Lin, Fulin Chen, Jihong Cui, Yuan Yu
Summary: The study conducted molecular cloning and characterization of the c-Fos gene in the Chinese fire-bellied newt, revealing its potential role in newt limb regeneration by modulating the expression of matrix metalloproteinase-3 during wound healing. These findings provide important insights into the mechanisms responsible for initiating newt limb regeneration.
Article
Plant Sciences
Bowen Chen, Changrong Li, Yingying Chen, Shengkan Chen, Yufei Xiao, Qi Wu, Lianxiang Zhong, Kaiyong Huang
Summary: The study identified proteins involved in early somatic embryogenesis (SE) of two Eucalyptus species, and highlighted potential regulatory networks related to high regeneration potential in Eucalyptus.
Review
Biochemistry & Molecular Biology
Yuzhou Gu, Ke Yao, Qiuli Fu
Summary: Lens regeneration is an ideal strategy for treating cataracts, with research focusing on in situ and in vitro techniques. The possibility of autologous stem cell regeneration of functional lenses has been confirmed, along with induced pluripotent stem cells forming lens-like structures in vitro. Understanding the complex signaling pathways is crucial for exploring the prospects of lens regeneration.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)