Article
Cell Biology
Ping Shi, Jia Tian, Julianne C. Mallinger, Dahao Ling, Loic P. Deleyrolle, Jeremy C. Mcintyre, Tamara Caspary, Joshua J. Breunig, Matthew R. Sarkisian
Summary: This study found that increasing ARL13B expression in glioma cells can promote the accumulation of SMO and GLI2 in cilia, and this accumulation is resistant to SMO inhibitors. Furthermore, the increase in ARL13B also leads to changes in ciliary membrane characteristics and retrograde transport, which further promote SMO and GLI accumulation in glioma cells.
Article
Biochemistry & Molecular Biology
Yueh-Chien Lin, Yuan-Li Huang, Ming-Hua Wang, Chih-Yu Chen, Wei-Min Chen, Yi-Cheng Weng, Pei-Yi Wu
Summary: Prostate cancer is a common and deadly cancer in aging men worldwide, and recent research suggests that calreticulin (CRT) plays an important role in its regulation. In this study, we investigated the effects of CRT on the expression of beta 1-integrin in prostate cancer cells and found that it affects beta 1-integrin expression through distinct regulatory mechanisms.
Article
Biochemistry & Molecular Biology
L. Michael Carastro, Ethan J. Vallebuona, Ricardo Cordova, Ashely N. Gannon, Seung Joon Kim, Corrine M. Costello, Ricardo A. Declet-Bauzo, Nagi Kumar, Jong Y. Park
Summary: Poly E, a green tea extract, has chemopreventive activity against PCa. It induces gene expression changes in PCa cells, with significant increases in MXD1 and RGS4 expression, suggesting that Poly E may exert its chemopreventive activity through the regulation of these genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Le Zhang, Sandrine Billet, Gabrielle Gonzales, Krizia Rohena-Rivera, Hayato Muranaka, Gina Chia-Yi Chu, Qian Yang, Hyung Kim, Neil A. Bhowmick, Bethany Smith
Summary: A high-fat diet is associated with prostate cancer progression, and cancer-associated fibroblasts (CAF) play an important role in this process. This study found that saturated fatty acids induce lineage plasticity reprogramming of prostate cancer through interaction with CAF via the Wnt5a and Hedgehog (Hh) signaling pathways. These findings provide relevant data to potentially improve the prognosis for patients with high fat intake.
Article
Biochemistry & Molecular Biology
Sung Pyo Hong, Kil Won Kim, Soon Kil Ahn
Summary: The Hedgehog (Hh) signaling pathway is critical for prostate cancer progression. GLI transcription factors regulate downstream targets to drive prostate cancer progression. The study found that DAX1 interacts with GLI1 and GLI2, inhibiting Hh signaling and reducing prostate cancer cell proliferation and viability. This sheds light on the complex regulation of Hh signaling and suggests DAX1 as a potential therapeutic target for prostate cancer.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Arundhasa Chandrabalan, Rithwik Ramachandran
Summary: Proteinase-activated receptors (PARs) in prostate cancer cells regulate tumor growth and metastasis through autocrine signaling. In this study, PAR1 and PAR2 were found to be functional in PC3 cells, while PAR4 was not expressed. The secretion of proteolytic enzymes by PC3 cells triggers cleavage of PARs, leading to autocrine signaling and the regulation of genes related to prostate cancer. PAR1 deficiency promotes cell migration and suppresses cell proliferation, while PAR2 deficiency has the opposite effects.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Editorial Material
Cell Biology
Li Xin
Summary: A recent study by Steiner et al. reveals that Ly6d(+) prostate tumor cells can survive after castration through an autocrine amphiregulin signaling pathway.
TRENDS IN CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ignazia Tusa, Sinforosa Gagliardi, Alessandro Tubita, Silvia Pandolfi, Alessio Menconi, Matteo Lulli, Persio Dello Sbarba, Barbara Stecca, Elisabetta Rovida
Summary: The study identified a novel Hedgehog-GLI-ERK5 axis that regulates melanoma cell growth, showing that activation of the Hedgehog-GLI pathway increased ERK5 expression and that inhibitors targeting both pathways were more effective in reducing melanoma cell viability and colony formation compared to single treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Chamey Suchors, James Kim
Summary: The Hedgehog signaling pathway is crucial for development and tissue regeneration, and its dysregulation has been linked to cancer. Our understanding of this pathway in tumorigenesis has significantly advanced over the past few decades. The pathway can have both tumor-suppressive and oncogenic functions, and noncanonical activation of GLI transcription factors has been observed in certain types of tumors.
Review
Biology
Fan Yang, Daniel T. Wynn, Chen Shen, Nagi G. Ayad, David J. Robbins
Summary: The Hedgehog signaling pathway is crucial for embryonic development and tissue homeostasis, but its abnormal activation is implicated in various cancers. GLI transcription factors act as the ultimate effectors of this pathway and their activity is regulated by multiple mechanisms. Recent studies have found that novel protein complexes can also regulate the transcriptional activity of GLI proteins, providing innovative druggable targets for GLI-dependent cancers.
Article
Biochemistry & Molecular Biology
Seksom Chainumnim, Audchara Saenkham, Kulvadee Dolsophon, Kittipong Chainok, Sunit Suksamrarn, Wanlaya Tanechpongtamb
Summary: Natural compounds from different parts of Momordica cochinchinensis showed significant inhibition against human prostate cancer cells. The stem extract demonstrated the highest cytotoxic and apoptotic effects, containing alpha-spinasterol, ligballinol, and fatty acids as its main constituents.
Article
Multidisciplinary Sciences
Marzena Swiderska-Syn, Julia Mir-Pedrol, Alexander Oles, Olga Schleuger, April D. Salvador, Sean M. Greiner, Cara Seward, Fan Yang, Benjamin R. Babcock, Chen Shen, Daniel T. Wynn, Avencia Sanchez-Mejias, Timothy R. Gershon, Vanesa Martin, Heather J. McCrea, Kathryn G. Lindsey, Carsten Krieg, Jezabel Rodriguez-Blanco
Summary: Single-cell transcriptomic analysis of medulloblastoma revealed that vismodegib treatment increased Sox2-expressing astrocyte-like cells. These Sox2(+) cells relied on Sonic Hedgehog (SHH) signaling, with MYC-dependent activation downstream of Smo. GLI inhibitors depleted resistant Sox2(+) cells, reduced their engraftment ability, and increased symptom-free survival.
Article
Oncology
Yibin Pan, Jiena Zhou, Weidan Zhang, Lili Yan, Meifei Lu, Yongdong Dai, Hanjing Zhou, Songying Zhang, Jianhua Yang
Summary: The study found up-regulation of SHH and accumulation of SQSTM1/P62 in epithelial ovarian cancer. Cyp induced autophagy through the PI3K/AKT signaling pathway, and low-dose Cyp and chloroquine (CQ) significantly promoted the migratory ability of SKOV3 cells. The findings suggest that inhibition of the SHH pathway and autophagy may be a potential and effective therapy for the treatment of ovarian cancer.
Article
Biochemistry & Molecular Biology
Ana Paola G. Lombardi, Renan P. Cavalheiro, Catarina S. Porto, Carolina M. Vicente
Summary: This study demonstrates that ER activates rapid response molecules such as SRC and PI3K/AKT to enhance the tumorigenic potential of prostate cancer cells, increasing cell proliferation, migration, invasion, and tumor formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dongkyu Jeon, Minjae Jo, Yechan Lee, So-Hyeon Park, Hong Thi Lam Phan, Joo Hyun Nam, Wan Namkung
Summary: ANO1, a calcium-activated chloride channel, plays a role in the proliferation, migration, and invasion of various cancer cells. In this study, the effects of two stereoisomers of resveratrol on ANO1 activity were investigated, and it was found that cis- and trans-resveratrol inhibited ANO1 activity with different potencies. Cis-resveratrol was shown to be a potent inhibitor of ANO1 and exhibited ANO1-dependent anticancer activity against human metastatic prostate cancer PC-3 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)