期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 372, 期 1, 页码 230-235出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.05.022
关键词
human adipose-derived stromal cells; (HADSC); mesenchymal stromal cell (MSC); mesenchymal stem cells; in vitro differentiation; chondrogenesis; TGF-beta 3; transcription factors; Runx2; Osterix
资金
- NHLBI NIH HHS [R01 HL073256, R01 HL073256-04] Funding Source: Medline
- NIAMS NIH HHS [R01 AR036994, R01 AR036994-22] Funding Source: Medline
- NIDCD NIH HHS [T32 DC000022] Funding Source: Medline
- PHS HHS [R01073256, R0136994] Funding Source: Medline
The aim of this study was to create a gene expression profile to better define the phenotype of human adipose-derived stromal cells (HADSCs) during in vitro chondrogenesis, osteogenesis and adipogenesis. A novel aspect of this work was the analysis of the same subset of genes during HADSC differentiation into all three lineages. Chondrogenic induction resulted in increased mRNA expression of Sox transcription factors, COL2A1, COL10A1, Runx2, and Osterix. This is the first report demonstrating significant upregulation in expression of osteogenesis-related transcription factors Runx2 and Osterix by TGF-beta 3 induction of HADSCs during in vitro chondrogenesis. These findings suggest that the commonly-used chondrogenic induction reagents promote differentiation suggestive of hypertrophic chondrocytes and osteoblasts. We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering. (C) 2008 Elsevier Inc. All rights reserved.
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