Article
Biochemistry & Molecular Biology
George Necula, Mihaela Bacalum, Mihai Radu
Summary: Using molecular dynamics simulations, the interaction between a short antimicrobial peptide (AMP) and lipopolysaccharides (LPS) from two models of E. coli outer membrane (OM) was investigated. The results showed that AMP insertion in the LPS layer was facilitated by the gap created by OmpF, forming hydrogen bonds with the inner core oligosaccharides. OM proteins, such as OmpF, may play a crucial role in the permeation of short AMPs across the OM by exposing the LPS binding site or facilitating direct translocation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Yi Wang, Yuchi Song, Shaonan Yan, Rina Hiramine, Yuki Ohnishi, Yuki Yokoi, Kiminori Nakamura, Takashi Kikukawa, Tokiyoshi Ayabe, Tomoyasu Aizawa
Summary: This study investigated the antibacterial mechanisms of Cryptdin-4 in different forms (oxidized and reduced states) under different oxygen conditions using Escherichia coli as a model. The results showed that both forms exhibited antimicrobial activity against bacteria under aerobic conditions through rapid membrane depolarization. Under anaerobic conditions, the ability to disrupt bacterial membrane function decreased, but the reduced form still maintained some antimicrobial activity, possibly through DNA binding inhibition of intracellular functions.
Article
Chemistry, Medicinal
Bing-Chang Lee, Jui-Che Tsai, Cheng-Yung Lin, Chun-Wei Hung, Jin-Chuan Sheu, Huai-Jen Tsai
Summary: The study demonstrates that transgenic Bacillus subtilis strains C117 and C166, expressing rCiMAM, exhibit higher bactericidal activity against halophilic bacteria, indicating promising potential in protecting marine fish and shellfish from halophilic bacterial infection.
Article
Biochemistry & Molecular Biology
Alicia Malone, Rikki F. Clark, David W. Hoskin, Melanie R. Power Coombs
Summary: Lactoferricin AMPs have immunomodulatory effects and may be used to treat inflammatory diseases.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Chemistry, Multidisciplinary
Liang Ma, Yongsheng Luo, Yong-Hao Ma, Xiaolin Lu
Summary: Antimicrobial peptides, such as CM15, interacted with a negatively charged phospholipid bilayer, DPPG, exhibiting rapid adsorption and efficient disruption due to increased positive charge from C-terminal amidation. Elevated temperatures promoted peptide penetration and eventual disruption of the entire bilayer due to enhanced fluidity. This study provides practical cues for designing high-efficiency antimicrobial peptides.
Article
Biotechnology & Applied Microbiology
Linglong Fu, Mengning Sun, Weizhang Wen, Na Dong, Defa Li
Summary: This study describes an efficient and simple method for extracellular production of Ulp1 tool enzyme using a leaky E. coli strain. It was found that the addition of glycine increased the extracellular production of Ulp1 approximately four fold. The extracellular Ulp1 had high activities for cleaving SUMO fusion proteins and the cleaved peptide pBD2 exhibited antimicrobial activity. This method of extracellular production of tool enzymes is cost-effective and has potential applications in pharmaceutical, industrial, and biotechnological fields.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Kun Yang, Yanan Shi, Yufang Li, Guangqiang Wei, Qiong Zhao, Aixiang Huang
Summary: BCp12 is a novel antibacterial peptide derived from casein with broad-spectrum antibacterial effects. It inhibits the growth of E. coli by causing metabolic disorders and disrupting the structure of cell membranes. The peptide binds to key enzymes involved in fatty acid degradation pathway, significantly inhibiting their activities.
Article
Biotechnology & Applied Microbiology
Nanakow Baiden, Chiara Gandini, Paul Goddard, Olga Sayanova
Summary: The increase in antibiotic resistance of bacterial strains is a major concern. Recent research has shown that the lysate of marine diatom Phaeodactylum tricornutum can significantly reduce the growth rate of Gram-negative bacteria. By engineering P. tricornutum, it was possible to enhance its antimicrobial activity against these bacteria. These results demonstrate that microalgae can be engineered to produce natural antimicrobial and anti-inflammatory compounds, providing an alternative to production-purpose antibiotics for treating serious bacterial infections.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Pawel Serafin, Pawel Kowalczyk, Adriano Mollica, Azzurra Stefanucci, Anna K. Laskowska, Magdalena Zawadzka, Karol Kramkowski, Patrycja Kleczkowska
Summary: Finding the ideal antimicrobial drug remains challenging, but amphibian-derived peptides have shown potential as broad-spectrum antibacterial agents. In this study, dermorphin and ranatensin peptides were combined to create a hybrid compound called LENART01. It exhibited superior antimicrobial activity against certain E. coli strains compared to clinically available antibiotics, but showed dose-dependent hemolytic effects in hemolytic assays.
Article
Microbiology
Lingling Zhou, Kaiqi Lian, Mengting Wang, Xueyi Jing, Yuanchen Zhang, Jinling Cao
Summary: This study demonstrated that LL-1 has a strong bactericidal effect on E. coli by increasing the permeability of the cell membrane, resulting in leakage of intracellular content. This finding is of great importance for further understanding the antibacterial mechanism of LL-1 against E. coli and its clinical applications.
Article
Biochemistry & Molecular Biology
Julia Bahja, Marcus K. Dymond
Summary: Oxidative stress affects lipid membranes by reducing stored curvature elastic energy, which can potentially be mitigated by compounds that increase stored curvature elastic energy; Age-related changes in lipid composition may represent a homeostatic response of cells to compensate for the accumulation of in vivo lipid oxidation products.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Fanqiang Meng, Yanan Liu, Ting Nie, Chao Tang, Fengxia Lyu, Xiaomei Bie, Yingjian Lu, Mingwen Zhao, Zhaoxin Lu
Summary: Antibiotic resistance is a global health concern. Plantaricin A (PlnA) enhances the potency of antibiotics and slows down the development of antibiotic resistance. Among the PlnA1 analogs, OP4 demonstrates good performance in the permeability of the bacterial outer membrane, cytotoxicity, and therapeutic index. Furthermore, OP4 heightens the potency of erythromycin against Escherichia coli and relieves inflammation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Da Dam Kang, Jonggwan Park, Yoonkyung Park
Summary: Antibiotic-resistant bacteria are a global health concern, and alternative treatments are needed. This study found that PN5 derived from pine needle exhibited antimicrobial and anti-inflammatory activities. PN5 showed the ability to disrupt bacterial cell membranes and did not induce resistance in bacteria. In addition, PN5 demonstrated the ability to suppress inflammatory responses.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Ingrid A. Edwards, Sonia T. Henriques, Mark A. T. Blaskovich, Alysha G. Elliott, Matthew A. Cooper
Summary: In this study, the mechanism of action of arenicin-3 and its synthetic analogue AA139 were investigated. The results showed that simple amino acid changes can affect their interaction with membranes, leading to changes in their potency, selectivity, and toxicity. AA139 demonstrated improved antibacterial activity and lower toxicity compared to arenicin-3, possibly due to its specific binding and insertion properties.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Acoustics
Shen Yang, Zijin Yuan, Jude Juventus Aweya, Shiying Huang, Shanggui Deng, Linfan Shi, Mingjing Zheng, Yueling Zhang, Guangming Liu
Summary: This study found that the in silico predicted antimicrobial peptide TGH2 combined with low-intensity ultrasound significantly reduced the minimum inhibitory concentration of Escherichia coli and enhanced bactericidal effect, as shown by transmission electron microscopy and circular dichroism for structural analysis.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sabrina Riedl, Regina Leber, Beate Rinner, Helmut Schaider, Karl Lohner, Dagmar Zweytick
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2015)
Article
Oncology
Sabrina Riedl, Beate Rinner, Helmut Schaider, Bernadette Liegl-Atzwanger, Katharina Meditz, Julia Preishuber-Pfluegl, Sarah Grissenberger, Karl Lohner, Dagmar Zweytick
Article
Multidisciplinary Sciences
Beate Rinner, Greta Gandolfi, Katharina Meditz, Marie-Therese Frisch, Karin Wagner, Alessia Ciarrocchi, Federica Torricelli, Raili Koivuniemi, Johanna Niklander, Bernadette Liegl-Atzwanger, Birgit Lohberger, Ellen Heitzer, Nassim Ghaffari-Tabrizi-Wizsy, Dagmar Zweytick, Iris Zalaudek
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Sabrina Riedl, Beate Rinner, Helmut Schaider, Karl Lohner, Dagmar Zweytick
Article
Multidisciplinary Sciences
Dagmar Zweytick, Bostjan Japelj, Eugenia Mileykovskaya, Mateja Zorko, William Dowhan, Sylvie E. Blondelle, Sabrina Riedl, Roman Jerala, Karl Lohner
Article
Multidisciplinary Sciences
Dagmar Kolb, Elisabeth Pritz, Bibiane Steinecker-Frohnwieser, Birgit Lohberger, Alexander Deutsch, Thomas Kroneis, Amin El-Heliebi, Gottfried Dohr, Katharina Meditz, Karin Wagner, Harald Koefeler, Gerd Leitinger, Andreas Leithner, Bernadette Liegl-Atzwanger, Dagmar Zweytick, Beate Rinner
Article
Multidisciplinary Sciences
Michaela Wenzel, Alina Iulia Chiriac, Andreas Otto, Dagmar Zweytick, Caroline May, Catherine Schumacher, Ronald Gust, H. Bauke Albada, Maya Penkova, Ute Kraemer, Ralf Erdmann, Nils Metzler-Nolte, Suzana K. Straus, Erhard Bremer, Doerte Becher, Heike Broetz-Oesterhelt, Hans-Georg Sahl, Julia Elisabeth Bandow
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Multidisciplinary Sciences
Christina Wodlej, Sabrina Riedl, Beate Rinner, Regina Leber, Carina Drechsler, Dennis R. Voelker, Jae-Yeon Choi, Karl Lohner, Dagmar Zweytick
Editorial Material
Biochemistry & Molecular Biology
Nermina Malanovic, Dagmar Zweytick, Georg Pabst
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Article
Biochemistry & Molecular Biology
Sarah Grissenberger, Sabrina Riedl, Beate Rinner, Regina Leber, Dagmar Zweytick
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Article
Chemistry, Multidisciplinary
Carolin Tetyczka, Sonja Hartl, Ramona Jeitler, Markus Absenger-Novak, Claudia Meindl, Eleonore Froehlich, Sabrina Riedl, Dagmar Zweytick, Eva Roblegg
Summary: Topical drug administration to the oral mucosa holds promise for treating inflammatory diseases, but changes in cell barriers must be considered. Inflammation induced alterations in cell membrane charge, leading to increased NLC absorption, cell detachment, and hyperplasia.
Article
Biochemistry & Molecular Biology
Theresa Maxian, Lisa Gerlitz, Sabrina Riedl, Beate Rinner, Dagmar Zweytick
Summary: The study shows that incorporating D-amino acids can enhance the stability of antitumor peptides, leading to effective antitumor activity against cancer cells such as melanoma and glioblastoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Silke Schrom, Thomas Hebesberger, Stefanie Angela Wallner, Ines Anders, Erika Richtig, Waltraud Brandl, Birgit Hirschmugl, Mariangela Garofalo, Claudia Bernecker, Peter Schlenke, Karl Kashofer, Christian Wadsack, Ariane Aigelsreiter, Ellen Heitzer, Sabrina Riedl, Dagmar Zweytick, Nadine Kretschmer, Georg Richtig, Beate Rinner
Summary: Melanomas present challenges in treatment during pregnancy, but through culturing and studying melanoma cells, new model systems and treatment options have been discovered, providing new possibilities for studying melanoma heterogeneity and pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Maximiliane Wussmann, Florian Kai Groeber-Becker, Sabrina Riedl, Dina Alihodzic, Daniel Padaric, Lisa Gerlitz, Alexander Stallinger, Bernadette Liegl-Atzwanger, Dagmar Zweytick, Beate Rinner
Summary: The host defense derived peptide RDP22 has shown promising potential against the highly aggressive NRAS mutated melanoma cell line MUG-Mel2, inducing cell death both in vitro and in vivo. The peptide effectively targets the tumor cells and leads to tumor regression and reduction in proliferation, highlighting its specificity and efficacy in combating melanoma.
Article
Multidisciplinary Sciences
Ellen Heitzer, Arwin Groenewoud, Katharina Meditz, Birgit Lohberger, Bernadette Liegl-Atzwanger, Andreas Prokesch, Karl Kashofer, Diana Behrens, Johannes Haybaeck, Dagmar Kolb-Lenz, Harald Koefeler, Sabrina Riedl, Helmut Schaider, Carina Fischer, B. Ewa Snaar-Jagalska, Danielle de'Jong, Karoly Szuhai, Dagmar Zweytick, Beate Rinner
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)