Article
Chemistry, Analytical
Tongtong Li, Yan Zhang, Xiaohong Sun, Yanjin Zhang, Yongan Wang, Zhiyong Nie
Summary: A fluorescent probe was developed for ratiometric detection of thallium ions in mineral water samples by modifying a G-rich aptamer. The probe changes its structure from a random coil to a G-quadruplex upon binding with Tl+, allowing for fluorescence resonance energy transfer between the donor and acceptor moieties. Under optimized conditions, the probe provided satisfactory results with a detection limit of 30.1 μM and a linear concentration range of 10 μM-10 mM.
Article
Biochemistry & Molecular Biology
Alexander V. Fonin, Sergey A. Silonov, Iuliia A. Antifeeva, Olga V. Stepanenko, Olesya V. Stepanenko, Anna S. Fefilova, Olga I. Povarova, Anastasia A. Gavrilova, Irina M. Kuznetsova, Konstantin K. Turoverov
Summary: BADAN fluorescence properties are determined by different excited states, undergo changes during protein structural alterations and solvent interactions, and can be quenched by certain amino acid residues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Yuxuan Zan, Yazhuo Kang, Bowei Wang, Siqian Cui, Zhuoyao Shen, Jiahui Shu, Xiangjin Kong, Ligong Chen, Xilong Yan, Yang Li
Summary: 2,4,6-Trinitrophenol (TNP) is an important and widely used chemical in industry. Due to its high toxicity, flammability, and explosive nature, even trace amounts of TNP can pose potential threats to ecosystems and human security. Therefore, it is crucial to establish a rapid and reliable method for TNP detection. In this study, a novel amphiphilic fluorescent probe, TPE-CD, was successfully designed and synthesized. TPE-CD exhibited excellent sensitivity and selectivity towards TNP, making it a promising tool for TNP detection.
Article
Biochemistry & Molecular Biology
Dayane Correia Gomes, Marcela Rodrigues Barros, Thais Meira Menezes, Jorge Luiz Neves, Patricia Maria Guedes Paiva, Teresinha Goncalves da Silva, Thiago Henrique Napoleao, Marilia Cavalcanti Coriolano, Maria Tereza dos Santos Correia
Summary: EgviL is a cytotoxic protein from Egletes viscosa plant, with toxic effect on leukemic cells and ability to bind to galactose and glucose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Hengzhi Zhao, Yazhou Liu, Jie Cui, Chunlei Yang, Na Gao, Jing Jing, Xiaoling Zhang
Summary: A DNA tetrahedron-based nanoprobe called DNA nanomimosa was designed for sensing RNase H activity with a low detection limit, good specificity, and advantages such as self-delivery ability and good biocompatibility. This design has potential applications in ribonuclease research and could be expanded to other biomedical research and clinical diagnostics.
Article
Chemistry, Analytical
Jinshan Yuan, Guoming Xie, Junjie Li, Yaxing Xie, Yuan Yao, Jianhong Zhang, Yulei Hou, Hui Chen
Summary: A fluorescent sensing platform was developed for the simple and sensitive monitoring of vancomycin, using an allosteric probe that undergoes a conformational change in the presence of vancomycin. This platform has the potential to support therapeutic drug monitoring of vancomycin, promoting the rational use of antibiotics in clinics.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hengzhi Zhao, Wei Hu, Jing Jing, Xiaoling Zhang
Summary: A facile and ratiometric strategy for detecting UDG activity using FRET was developed in this study, which can detect UDG activity and screen UDG inhibitors without requiring additional signal amplification. By measuring system fluorescence intensity and exploiting FRET signal difference, the detection limit is 0.087 U/mL.
Article
Biophysics
Xianghan Zhang, Xiaohan Gao, Jialin Zhou, Zhiqing Gao, Yingdi Tang, Zuhong Tian, Pengbo Ning, Yuqiong Xia
Summary: The albumin-based FRET nanoprobe Cy5/7@HSA NPs showed high sensitivity and excellent imaging in cell and tumor imaging, indicating its strong potential as a multileveled tumor imaging platform.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Spectroscopy
Pai Liu, Yu-ling Liu, He Huang, Guang Bai, Yong-jin Peng
Summary: The level of hydrogen sulfide (H2S) in the human body is associated with various diseases. Therefore, the detection of H2S level in biological systems is important and has attracted attention from researchers. This study thoroughly investigated the mechanisms of a highly efficient fluorescent probe based on the Fluorescence Resonance Energy Transfer (FRET), providing insights for designing new probes applicable in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Chemistry, Multidisciplinary
Xiaotong Shen, Wei Xu, Jin Ouyang, Na Na
Summary: Fluorescence resonance energy transfer (FRET) combined with nanomaterials holds great potential for precise measurements in biological systems.
CHINESE CHEMICAL LETTERS
(2022)
Review
Plant Sciences
Ruphi Naz, Andleeb Khan, Badrah S. Alghamdi, Ghulam Md Ashraf, Maimonah Alghanmi, Altaf Ahmad, Sheikh Shanawaz Bashir, Qazi Mohd Rizwanul Haq
Summary: Ionotropic glutamate receptors (iGluRs) play an important role in neurons, with involvement in memory, neuronal development, and synaptic plasticity. Arabidopsis thaliana has genes comparable to human iGluRs, affecting plant growth and physiological activities. The evolutionary relationship between animal and plant glutamate receptors suggests a correlation, which can be utilized for early detection of neurodegenerative diseases. This review updates knowledge on Arabidopsis glutamate receptors, their evolution, functions, and expression, in comparison with iGluRs. Additionally, the use of FRET nanosensors for understanding neurotransmitter signaling via glutamate receptors in animals and plants is discussed.
Article
Biophysics
Nicole L. Walker, Jeffrey E. Dick
Summary: The development of a new electrochemical technique - open circuit potential, has been aimed at stable and reliable monitoring of biological metabolites. By immobilizing oxidase enzyme in chitosan hydrogel, the method achieves specificity in monitoring metabolites. This approach can effectively detect metabolites such as glucose and can be extended to detect other analytes.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Soumyadip Bhunia, Kaustav Gangopadhyay, Ashutosh Ghosh, Sourav Kanti Seth, Rahul Das, Pradipta Purkayastha
Summary: Rigidifying the protecting ligands with arginine substantially enhances the fluorescence quantum yield of ligand-protected gold nanoclusters, enabling their use as energy donors in FRET processes with hemicyanine dyes for potential applications in biological cells.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Amr Ghazy, Jonas Ylonen, Nagarajan Subramaniyam, Maarit Karppinen
Summary: We have developed a novel atomic/molecular layer deposition (ALD/MLD) process for europium-organic thin films using Eu(thd)3 and 2-hydroxyquinoline-4-carboxylic acid (HQA) precursors. The resulting Eu-HQA thin films demonstrate high growth rate and exhibit red Eu3+ luminescence with extended excitation wavelength range up to about 400 nm, attributed to the sensitizer role of organic HQA. These films are further deposited on nanoplasmonic structures to achieve twentyfold enhanced emission intensity. Moreover, we demonstrate the potential of Eu-HQA coated nanoplasmonic structures in novel bioimaging applications through FRET-type energy transfer process with commercial Alexa647 fluorophor.
Article
Spectroscopy
Jiayu Zhang, Shanshan Hou, Jiaxin Zhang, Ning Liang, Longshan Zhao
Summary: A fast and simple aptamer-based fluorescence strategy using single-wall carbon nanohorns (SWCNHs) as a quencher for dopamine detection has been developed in this study. The innovative detection system shows a detection limit of 5 μM and a linear detection range of 0.02-2.20 mM, and has been successfully applied to detect dopamine in spiked serum samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Cell Biology
Giuseppe Maltese, Paraskevi-Maria Psefteli, Benedetta Rizzo, Salil Srivastava, Luigi Gnudi, Giovanni E. Mann, Richard C. M. Siow
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2017)
Review
Biochemistry & Molecular Biology
Manpreet K. Sagoo, Luigi Gnudi
FREE RADICAL BIOLOGY AND MEDICINE
(2018)
Article
Endocrinology & Metabolism
Nikolaos Fountoulakis, Giuseppe Maltese, Luigi Gnudi, Janaka Karalliedde
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2018)
Article
Endocrinology & Metabolism
Karen L. Onions, Monica Gamez, Nicola R. Buckner, Sian L. Baker, Kai B. Betteridge, Sara Desideri, Benjamin P. Dallyn, Raina D. Ramnath, Chris R. Neal, Louise K. Farmer, Peter W. Mathieson, Luigi Gnudi, Kari Alitalo, David O. Bates, Andrew H. J. Salmon, Gavin I. Welsh, Simon C. Satchell, Rebecca R. Foster
Article
Medicine, General & Internal
Hiddo J. L. Heerspink, Hans-Henrik Parving, Dennis L. Andress, George Bakris, Ricardo Correa-Rotter, Fan-Fan Hou, Dalane W. Kitzman, Donald Kohan, Hirofumi Makino, John J. V. McMurray, Joel Z. Melnick, Michael G. Miller, Pablo E. Pergola, Vlado Perkovic, Sheldon Tobe, Tingting Yi, Melissa Wigderson, Dick de Zeeuw
Article
Endocrinology & Metabolism
Ivan Hernandez-Diaz, Jiaqi Pan, Carlo Alberto Ricciardi, Xiaoyan Bai, Jianting Ke, Kathryn E. White, Maria Flaquer, Georgia E. Fouli, Fulye Argunhan, Anthea E. Hayward, Fan Fan Hou, Giovanni E. Mann, Robert Q. Miao, David A. Long, Luigi Gnudi
Review
Cell Biology
Carlo Alberto Ricciardi, Luigi Gnudi
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Cell Biology
Saima Ajaz, Mark J. McPhail, Luigi Gnudi, Francesca M. Trovato, Salma Mujib, Salvatore Napoli, Ivana Carey, Kosh Agarwal
Summary: This study demonstrates for the first time the association of NAFLD progression with mitochondrial dysfunction and changes in metabolites related to the urea cycle. A unique panel of mitochondrial-based signatures was identified to differentiate between stages of NAFLD progression.
Review
Medicine, General & Internal
Carlo Alberto Ricciardi, Luigi Gnudi
Summary: Cardiorenal syndrome in diabetes is characterized by cardiovascular and kidney alterations due to chronic metabolic and hemodynamic perturbations. Imbalance of vascular growth factors plays a crucial role in the progression of vascular dysfunction and organ damage in diabetes-related cardiorenal syndrome.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Review
Medicine, General & Internal
Luigi Gnudi
Article
Cardiac & Cardiovascular Systems
Luigi Gnudi, Nikolaos Fountoulakis, Angeliki Panagiotou, Antonella Corcillo, Giuseppe Maltese, Maria Flaquer Rife, Ioannis Ntalas, Russell Franks, Amedeo Chiribiri, Salma Ayis, Janaka Karalliedde
Summary: This study explored the effects of active vitamin-D (calcitriol) treatment on left ventricular mass in patients with type-2 diabetes and chronic kidney disease. The results showed that calcitriol treatment did not improve left ventricular mass index. Therefore, the routine use of active vitamin-D does not provide LVMI regression or cardiovascular protection in patients with type-2 diabetes and chronic kidney disease.
AMERICAN HEART JOURNAL
(2023)
Review
Urology & Nephrology
Georgia E. Fouli, Luigi Gnudi
Article
Endocrinology & Metabolism
Gareth S. D. Purvis, Fausto Chiazza, Jianmin Chen, Rodrigo Azevedo-Loiola, Lukas Martin, Dennis H. M. Kusters, Chris Reutelingsperger, Nikolaos Fountoulakis, Luigi Gnudi, Muhammed M. Yaqoob, Massimo Collino, Christoph Thiemermann, Egle Solito
Article
Medicine, General & Internal
Mahen Wijesuriya, Nikolaos Fountoulakis, Nicola Guess, Sarath Banneheka, Laksha Vasantharajah, Martin Gulliford, Giancarlo Viberti, Luigi Gnudi, Janaka Karalliedde
Article
Endocrinology & Metabolism
Giuseppe Maltese, Nikolaos Fountoulakis, Richard C. Siow, Luigi Gnudi, Janaka Karalliedde
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)