Article
Microbiology
Zhaolan Ding, Teresa M. Lamb, Ahmad Boukhris, Rachel Porter, Deborah Bell-Pedersen
Summary: In the model fungus Neurospora crassa, the circadian clock regulates rhythmic protein synthesis by controlling the phosphorylation and dephosphorylation of a conserved translation initiation factor, eIF2 alpha. This temporal coordination of phosphorylation and dephosphorylation events allows for the partitioning of translation to the night, which helps coordinate energy metabolism with protein synthesis and cellular growth.
Review
Cell Biology
Mauricio M. Oliveira, Eric Klann
Summary: This review discusses the requirement of de novo protein synthesis in memory formation and its mechanisms. It also introduces the regulation of local dendritic and axonal translation and its impact on memory consolidation. The review emphasizes the importance of eIF2α-dependent translation initiation in synaptic plasticity and memory formation, and explores the role of aberrant eIF2α phosphorylation in Alzheimer's disease.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zuben P. Brown, Irina S. Abaeva, Swastik De, Christopher U. T. Hellen, Tatyana Pestova, Joachim Frank
Summary: This study presents cryo-EM structures of hepatitis C virus IRES initiation complexes, revealing the dynamic network of 40S/IRES contacts and the conformational changes during the transition from eIF2- to eIF5B-containing 48S complexes, providing important insights into the process of subunit joining.
Article
Microbiology
Heather A. Walters, Brenda H. Welter, Harrison C. Moss, Martha A. Villano, Ronny Orobio-Hurtado, William J. Sullivan, Lesly A. Temesvari
Summary: This study identified two eIF2 alpha kinases in E. invadens that may regulate the stress response of Entamoeba, including oxidative stress, stage conversion, and virulence.
Article
Cell Biology
Simon Lizarazo, Yeeun Yook, Nien-Pei Tsai
Summary: Alzheimer's disease is a common form of dementia, characterized by the accumulation of amyloid beta peptide. This study reveals that Fmr1 is involved in A beta-induced translational suppression, leading to hyposynchrony of neuronal firing activity and loss of excitatory synapses. The mechanism involves the repression of phosphatases by Fmr1, resulting in translational suppression.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ronald C. Wek, Tracy G. Anthony, Kirk A. Staschke
Summary: Organisms adapt to changing environments by engaging cellular stress response pathways, such as the integrated stress response (ISR). This review focuses on the mechanisms of eIF2 alpha kinases as first responders of stress, the regulation of eIF2 alpha phosphorylation and other stress signals, and the differential mRNA translation triggered by the ISR. It also discusses the synergy between the ISR and mTORC1 in amino acid insufficiency, as well as the pathological conditions and therapeutic strategies related to aberrant regulation of the ISR.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Maria Teresa Borrello, Patricia Santofimia-Castano, Marco Bocchio, Angela Listi, Nicolas Fraunhoffer, Philippe Soubeyran, Eric Chevet, Christopher Pin, Juan Iovanna
Summary: This study uncovers a new role for NUPR1 in the unfolded protein response and the integrated stress response, involving interaction with eIF2 alpha to promote efficient stress response. Loss of NUPR1 leads to sustained eIF2 alpha phosphorylation and slower poststress protein synthesis recovery in cells.
Article
Virology
Barkha Ramnani, Shelby Powell, Adarsh G. Shetty, Praveen Manivannan, Brian R. Hibbard, Douglas W. Leaman, Krishnamurthy Malathi
Summary: Virus infection activates stress granule (SG) formation and interferes with the assembly of SG, indicating the important role of SG in antiviral defense. In this study, we found that fish cells respond to VHSV infection by forming SG, which requires PERK kinase and is essential for IFN production, antiviral signaling, and viral replication. Furthermore, the absence of G3BP1 leads to increased production of IFN, antiviral genes, and viral mRNA, but reduced viral protein synthesis and viral titers. Our findings highlight the critical role of ISR pathway activation and SG formation in regulating VHSV protein translation and replication.
Article
Veterinary Sciences
Ting Zhu, Xueli Jiang, Hangkuo Xin, Xiaohui Zheng, Xiaonuan Xue, Ji-Long Chen, Baomin Qi
Summary: PRV induces GADD34 expression to promote eIF2 alpha dephosphorylation, maintaining protein synthesis and facilitating viral replication.
VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Zhongyan Wu, Fan Mei, Yangyang Gan, Anhang Liu, Jiapan Hu, Yan Jin, Yuxin Yin
Summary: This study shows that FAM69C promotes stress granule assembly through phosphorylation of eIF2 alpha. Knockout of Fam69c in microglia leads to aberrant stress granule assembly and neuroinflammation. The findings suggest that FAM69C plays a critical role in regulating microglia function and stress response.
Article
Biochemistry & Molecular Biology
Ansul Lokdarshi, Albrecht G. von Arnim, Teressa K. Akuoko
Summary: The protein kinase GCN2 and its phosphorylation target eIF2 alpha play a key role in the plant's integrated stress response, regulating translation under stressful conditions. Recent studies show that GCN2 in Arabidopsis is controlled by reactive oxygen species from the chloroplast under various abiotic stresses like excess light.
PLANT SIGNALING & BEHAVIOR
(2022)
Article
Virology
Junchao Shi, Zi Li, Rongyi Xu, Jing Zhang, Qianyu Zhou, Rui Gao, Huijun Lu, Yungang Lan, Kui Zhao, Hongbin He, Feng Gao, Wenqi He
Summary: This study demonstrates that PHEV infection induces ER stress and activates the unfolded protein response (UPR), which suppresses viral replication through the PERK/PKR-eIF2 alpha axis. Understanding coronavirus-induced ER stress and UPR activation may provide insights into the pathogenic mechanisms of coronaviruses and aid in developing new treatment strategies.
JOURNAL OF VIROLOGY
(2022)
Review
Cell Biology
Alyssa M. English, Katelyn M. Green, Stephanie L. Moon
Summary: The integrated stress response allows eukaryotic cells to rapidly adapt to stress by globally suppressing translation initiation activity and selectively translating stress-induced genes, such as activating transcription factor 4 (ATF4). This mechanism involves the assembly of translationally repressed mRNAs and noncoding RNAs into cytoplasmic RNA-protein granules, and stabilizing polyadenylated RNAs. The reprogramming of gene expression during ISR is governed by regulated changes in mRNA translation, stability, and localization, with mutant alleles of key translation factors in the ISR pathway implicated in genetic disorders.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Article
Medicine, Research & Experimental
Wenzhong Zheng, Kexin Wang, Yachen Wu, Ge Yan, Chi Zhang, Zhiqiang Li, Lianrong Wang, Shi Chen
Summary: C9orf72 interacts with eIF2 alpha to modulate translation initiation; C9orf72 knockout leads to global translation inhibition and primary ER stress; C9orf72 delays SG formation in stressed cells.
Article
Microbiology
Han Zhong, Shuai Fan, Yongkun Du, Yuhang Zhang, Angke Zhang, Dawei Jiang, Shichong Han, Bo Wan, Gaiping Zhang
Summary: African swine fever (ASF) is a highly contagious and lethal disease of pigs caused by ASF virus (ASFV). In this study, the researchers investigated how ASFV MGF110-7L interacts with the eIF2 alpha signaling axis to control host translation and induce stress responses. They found that MGF110-7L triggers eIF2 alpha phosphorylation, translation suppression, and stress granule formation. Furthermore, MGF110-7L induces ER stress and the unfolded protein response, leading to translation reprogramming. These findings provide important insights into the translation regulation during ASFV infection and could guide the development of antiviral strategies.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Koki Itooka, Kazuo Takahashi, Shingo Izawa
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Toyokazu Nakamura, Vo Thi Anh Nguyet, Sae Kato, Yasuhiro Arii, Toshiharu Akino, Shingo Izawa
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2017)
Article
Biotechnology & Applied Microbiology
Yoko Ishida, Trinh Thi My Nguyen, Shingo Izawa
JOURNAL OF BIOTECHNOLOGY
(2017)
Article
Microbiology
Nozomi Kawazoe, Yukio Kimata, Shingo Izawa
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Koki Itooka, Kazuo Takahashi, Yukio Kimata, Shingo Izawa
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Cell Biology
Sena Homoto, Shingo Izawa
JOURNAL OF CELL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Trinh Thi My Nguyen, Yoko Ishida, Sae Kato, Aya Iwaki, Shingo Izawa
Article
Biotechnology & Applied Microbiology
Sae Kato, Yukina Yamauchi, Shingo Izawa
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Masashi Yoshida, Sae Kato, Shizu Fukuda, Shingo Izawa
Summary: The study found that pretreatment with mild ethanol upregulated the expression of proteins involved in PQC and mitigated the accumulation of insoluble proteins, even under severe ethanol stress. These results provide novel insights into ethanol tolerance and the adaptive capacity of yeast cells, especially during the brewing process.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Masashi Yoshida, Noboru Furutani, Futsuki Imai, Takeo Miki, Shingo Izawa
Summary: This study investigated the levels of insoluble proteins in wine yeast under laboratory conditions and during fermentation. Under laboratory conditions, both wine yeast cells and laboratory strain cells accumulated insoluble proteins under acute 10% (vol/vol) ethanol stress, which was suppressed by pretreatment with 6% (vol/vol) ethanol. During fermentation, the levels of insoluble proteins were maintained at low levels in wine yeast even when the ethanol concentration in SGM exceeded 10% (vol/vol). These findings provide further understanding of yeast cell physiology during winemaking.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Shizu Fukuda, Yasuhiro Sakurai, Shingo Izawa
Summary: Cold atmospheric plasma (CAP) irradiation has a sterilizing effect and can detoxify pesticide residues without damaging agricultural products. This study found that CAP irradiation could detoxify thiabendazole (TBZ) and cause little damage to the edible parts of mandarin oranges. The results indicate that CAP irradiation is a useful and effective means of maintaining food safety.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Cell Biology
Vo Thi Anh Nguyet, Ryoko Ando, Noboru Furutani, Shingo Izawa
Summary: Due to the lack of research on yeast physiology in low temperature range, most findings are based on analyses conducted at moderate temperatures. However, recent studies have found that severe ethanol stress does not inhibit proteasomal proteolysis in yeast cells at low temperatures, indicating that proteasomal activity can be maintained.
Review
Biotechnology & Applied Microbiology
Noboru Furutani, Shingo Izawa
Summary: This paper discusses the adaptability of yeast cells to ethanol stress and describes the adverse effects of ethanol on proteins and the responses of yeast cells. Recent studies on the acquired resistance of wine yeast cells to severe ethanol stress are also discussed.
FEMS YEAST RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yuka Adachi, Mana Umeda, Asako Kawazoe, Tetsuya Sato, Yasuyuki Ohkawa, Sakihito Kitajima, Shingo Izawa, Ikuko Sagami, Shigeru Taketani
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2017)
Article
Biochemistry & Molecular Biology
Taka-aki Takeda, Machiko Sasai, Yuka Adachi, Keiko Ohnishi, Jun-ichi Fujisawa, Shingo Izawaa, Shigeru Taketani
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)