Article
Cell Biology
Jialing Wang, Xiaodan Liu, Haixia Wang, Lili Qin, Anhua Feng, Daoxin Qi, Haihua Wang, Yao Zhao, Lihua Kong, Haiying Wang, Lin Wang, Zhenbo Hu, Xin Xu
Summary: The histone demethylase JMJD1C is associated with human platelet counts and plays a role in megakaryopoiesis. The study used various models and samples to investigate the expression and function of JMJD1C. The results suggest that JMJD1C may regulate megakaryopoiesis by influencing the actin network.
Article
Biology
Qian Ma, Minyi Zhao, Bing Long, Haixia Li
Summary: Acute myeloid leukemia (AML) is a common type of acute leukemia in adults, but refractory and drug resistance remains a challenge in its clinical treatment. Abnormal gene expression and epigenetic changes are important factors in the development of AML. This study identified the super-enhancer-associated gene CAPG, which is associated with poor prognosis in AML. Through proteomic and epigenomic analysis, it was found that CAPG regulates the NF-kappa B signaling pathway. Knockdown of Capg in an AML mouse model resulted in exhausted AML cells and improved survival. In conclusion, CAPG plays a role in AML progression through NF-kappa B signaling.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mohamed El-Tanani, Hamdi Nsairat, Vijay Mishra, Yachana Mishra, Alaa A. A. Aljabali, Angel Serrano-Aroca, Murtaza M. Tambuwala
Summary: Ran is a member of the Ras superfamily of proteins, regulating nucleocytoplasmic trafficking and mitosis by regulating spindle formation and nuclear envelope reassembly. Aberrant Ran expression in cancer is due to upstream dysregulation and abnormal activation of signaling pathways. Overexpression of Ran affects cell phenotype and has been found in various cancers, correlating with tumor grade and metastasis. Increased dependence on Ran for spindle formation and mitosis, as well as dysregulation of nucleocytoplasmic transport, contribute to the increased malignancy and invasiveness associated with Ran overexpression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
HaiYan Wang, Jinrong Hu, Kexi Yi, Zhiming Ma, XinJie Song, Yaelim Lee, Petr Kalab, Alexander D. Bershadsky, Yansong Miao, Rong Li
Summary: In this study, the recruitment of FMN2 to the ER around the spindle and its activity regulation in mouse meiosis I oocytes were investigated. The results showed that this process is regulated by the Ran GTPase and the ER-associated protein VAPA. The binding of FMN2 to importin a1/b complex and its subsequent disruption by RanGTP were required for FMN2 accumulation near the chromatin and the MI spindle. The importin-free FMN2 was then recruited to the ER surface around the spindle through its interaction with VAPA. Moreover, autoinhibition of FMN2 through an intramolecular interaction between its SLD and FH2 domain was relieved by the binding of VAPA, leading to localized actin polymerization. This study sheds light on the crucial role of FMN2 in the maturation of mammalian female gametes.
Article
Cell Biology
Imge Ozugergin, Karina Mastronardi, Chris Law, Alisa Piekny
Summary: This study uncovers the different cytokinesis kinetics and regulation mechanisms in AB cells and P-1 cells in Caenorhabditis elegans embryos.
JOURNAL OF CELL SCIENCE
(2022)
Article
Virology
Robert L. Harrison, Daniel L. Rowley
Summary: In this study, we analyzed the genome of a novel Alphabaculovirus from the rice case bearer in Kerala, India. The analysis revealed that the virus belongs to the group I clade of viruses in the genus Alphabaculovirus, but its placement within the group was uncertain. The genome also contained a unique ORF encoding a homolog of Ran GTPase, suggesting recent horizontal gene transfer from a lepidopteran host.
Article
Cell Biology
Eriko Hamasaki, Natsuki Wakita, Hiroki Yasuoka, Hikaru Nagaoka, Masayuki Morita, Eizo Takashima, Takayuki Uchihashi, Tetsuya Takeda, Tadashi Abe, Ji-Won Lee, Tadahiro Iimura, Moin A. Saleem, Naohisa Ogo, Akira Asai, Akihiro Narita, Kohji Takei, Hiroshi Yamada
Summary: This study investigates the molecular mechanisms underlying the regulation of actin by dynamin in human podocytes. The results suggest that the affinity of the K562E mutant for lipid membranes and its atypical self-assembling properties lead to actin disorganization in podocytes. Furthermore, the interaction between actin and membranes mediated by dynamin is critical for actin bundle formation and podocyte morphology and functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Roshan Kulkarni, Yudi Zhang, Steven B. Cannon, Karin S. Dorman
Summary: Genotyping by sequencing is a powerful tool for studying genetic variation in plants, but it becomes challenging in allopolyploid plants due to homoeologous similarity. This study introduces a new method called Comprehensive Allopolyploid Genotyper (CAPG), which uses an explicit likelihood to accurately determine subgenomic origin of reads in allopolyploids and reduces false heterozygous calls.
Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Tina Perica, Christopher J. P. Mathy, Jiewei Xu, Gwendolyn M. Jang, Yang Zhang, Robyn Kaake, Noah Ollikainen, Hannes Braberg, Danielle L. Swaney, David G. Lambright, Mark J. S. Kelly, Nevan J. Krogan, Tanja Kortemme
Summary: This study reveals that the functional specificity of the small GTPase switch protein Gsp1 in Saccharomyces cerevisiae is linked to the differential sensitivity of biological processes to different kinetics of the Gsp1 switch cycle. By making targeted point mutations to individual protein interaction interfaces of Gsp1 and conducting quantitative genetic and physical interaction mapping, it is shown that interface mutations allosterically tune the GTPase cycle kinetics, rather than grouping by targeted interfaces. These findings suggest a model in which protein partner binding or post-translational modifications may act as allosteric regulators of GTPase switching and may have implications for disease mutations targeting central molecular switches.
Article
Cell Biology
Huaiping Cui, Zhaoyu Jiang, Shujie Zeng, Hao Wu, Zihao Zhang, Xiaobo Guo, Kangdi Dong, Jinshen Wang, Liang Shang, Leping Li
Summary: In this study, a novel lncRNA WFDC21P derived from pseudogenes was identified and found to promote proliferation and metastasis of gastric cancer cells by activating the Akt/GSK3 beta/beta-catenin pathway. It may serve as a potential biomarker and therapeutic target for gastric cancer.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Danielle E. Talbot, Bailey J. Vormezeele, Garrett C. Kimble, Dylane M. Wineland, Daniel J. Kelpsch, Michelle S. Giedt, Tina L. Tootle
Summary: Prostaglandins (PGs) are locally acting lipid signals that regulate female reproduction, including oocyte development. The cellular mechanisms of PG action, particularly in relation to nucleolar function, are not well understood. In this study, the researchers used the Drosophila oogenesis system to investigate how PGs regulate nucleolar activity. They found that PG loss leads to changes in nucleolar morphology and increased rRNA transcription and protein translation. Further investigation revealed that PGs modulate nucleolar functions by regulating nuclear actin levels. Manipulations that increase nuclear actin result in round nucleolar morphology and increased transcription, suggesting that PGs carefully balance nuclear actin levels to control nucleolar activity in oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hugh Mulvey, Liam Dolan
Summary: The RHO GTPase proteins, including ROP in plants, play a crucial role in regulating cell polarity and geometry during tissue development and organogenesis. The loss-of-function mutants of the ROP gene in M. polymorpha exhibit defective tissue development and organogenesis due to impaired polarized cell growth and cell division orientation.
Article
Agronomy
Qi Mi, Hongqian Pang, Feishi Luan, Peng Gao, Shi Liu
Summary: The scc locus of the watermelon seed coat crack trait was fine mapped on chromosome 3, and Cla97C03G056110 was identified as the most likely candidate gene. The expression of Cla97C03G056110 was higher in non-scc lines and specifically expressed in seed coat tissue.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Theresa Lauster, Dorothee Stoeckle, Katharina Gabor, Theresa Haller, Natalie Krieger, Pia Lotz, Ravikumar Mayakrishnan, Ellen Spaeth, Steffi Zimmermann, Pantelis Livanos, Sabine Mueller
Summary: In this study, the role of PLECKSTRIN HOMOLOGY GTPase ACTIVATING PROTEINS (PHGAPS) in pavement cell shape establishment was investigated. It was found that PHGAPS interacted with ROP2 and showed distinct enrichment in the indentation regions of pavement cells. This localization was established upon undulation initiation and was maintained throughout cell expansion. These findings highlight the importance of PHGAPS in the establishment of ROP activity gradients and the coordinated establishment of multipolarity in epidermal cells.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)