Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Sahana Vasudevan, Parthasarathy Srinivasan, Prasanna Neelakantan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Summary: In this study, a novel photoluminescence-based assay using cysteamine-capped titanium oxide nanoparticles was developed for the rapid and sensitive diagnosis of microbial infections. The system showed a maximum relative response of 98% for AHLs produced by Pseudomonas aeruginosa, with a linear detection range of 10-160 nM in simulated host urine. The efficacy and specificity of the system in detecting AHLs produced by P. aeruginosa were confirmed in a temporal manner.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Wei Cheng, Zhenyu Wang, Yi Xiong, Zhifeng Wu, Xiang Tan, Yapeng Yang, Hang Zhang, Xi Zhu, Hong Wei, Shiyu Tao
Summary: This study demonstrated the detrimental effects of 3OC12 on intestinal health in mice, highlighting the role of gut microbiome in the induced intestinal damage. Furthermore, the results showed that fecal microbial transplantation could reproduce the effects of 3OC12 challenge in SPF mice in GF mice.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Raman Pachaiappan, Tharun Prasanna Rajamuthu, Ananya Sarkar, Pradiksha Natrajan, Nagasathiya Krishnan, Meenakumari Sakthivelu, Palaniyandi Velusamy, Palaniappan Ramasamy, Subash C. B. Gopinath
Summary: Flavonoids and isoflavonoids are effective inhibitors of biofilm formation and virulence factor production in Pseudomonas aeruginosa, with acacetin exhibiting the strongest inhibition capacity. These compounds downregulate the expression of virulence genes, making them potential targets for anti-quorum sensing strategies.
PROCESS BIOCHEMISTRY
(2022)
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: In the bacterium Pseudomonas aeruginosa, quorum sensing (QS) can activate the expression of dozens to hundreds of genes. In this study, the researchers investigated how the size and content of the QS regulon can evolve during long-term growth of P. aeruginosa. They found that the QS regulon can undergo a reductive adaptation in response to continuous QS-dependent growth. These findings provide insights into the strain-to-strain variability in the size and content of the P. aeruginosa QS regulon.
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: This study investigates the evolution of the QS regulon in Pseudomonas aeruginosa during long-term growth when cells require some but not all the functions activated by QS. The research reveals that the QS-regulon in P. aeruginosa can undergo a reductive adaptation in response to continuous QS-dependent growth, providing insights into the variability in the size and content of the QS regulon in different strains.
Article
Cell Biology
Shiyu Tao, Yi Xiong, Dandan Han, Yu Pi, Hanlu Zhang, Junjun Wang
Summary: Microbes use quorum sensing (QS) autoinducers for population communication, with N-(3-oxododecanoyl)- l-homoserine lactone (3OC12) from Pseudomonas aeruginosa LasI-LasR system having deleterious effects on intestinal epithelial cells. 3OC12 and 3OC14 trigger apoptosis rather than necrosis and ferroptosis in intestinal epithelial cells, reduce ECM-receptor interaction pathway gene expression, and decrease protein expressions from ECM and tight junction-associated pathway. Furthermore, 3OC12 and 3OC14 disrupt the intestinal epithelial barrier by inducing oxidative stress and apoptosis, with NAC showing potential for alleviating the syndrome caused by QS autoinducers exposure.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Environmental Sciences
Bernadett Zara, Mate Polgar, Gyoergy Sipos, Gabor Doka, Parag Gogate, Vladimir Djokovic, Levente Csoka
Summary: Hydrodynamic cavitation treatment can induce the inactivation of bacterial signal molecules through shear effects and hydroxyl radicals, with the strongest effects achieved at speeds > 2000 rpm, consistent with literature data.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Ankita Chopra, Dattatray Mongad, Surekha Satpute, Pranab Behari Mazumder, Praveen Rahi
Summary: The study aimed to isolate and characterize tea rhizobacteria that produce quorum sensing molecules and exhibit multiple plant growth-promoting activities. Thirty-one isolates were obtained from the tea rhizosphere, and two isolates (RTE1 and RTE4) were selected for their ability to inhibit tea fungal pathogens and produce AHL molecules. Genome-based analysis confirmed RTE1 as Burkholderia cepacia and RTE4 as Pseudomonas aeruginosa. Detailed characterization of the AHLs produced by the isolates revealed different chain lengths. Confocal microscopy showed biofilm formation by RTE1 and RTE4. Several genes involved in quorum sensing, plant growth promotion, and inducing systemic resistance were identified. Based on their functional traits, B. cepacia RTE1 and P. aeruginosa RTE4 are promising candidates for enhancing the health and growth of tea plantations.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
M. de Celis, L. Serrano-Aguirre, I Belda, R. Liebana-Garcia, M. Arroyo, D. Marquina, I de la Mata, A. Santos
Summary: The study indicates that the use of AHLs-degrading enzymes can reduce biofilm formation on WWTP membranes and ameliorate the virulence of Gram-negative bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Plant Sciences
Siqi Liu, Xixian Zhu, Zhenchen Yan, Hui Liu, Lianhui Zhang, Wenjuan Chen, Shaohua Chen
Summary: Quorum sensing is a communication mechanism used by microorganisms to regulate population density and behavior. Quorum quenching, which interferes with this communication, shows promise in plant disease control. In this study, a quenching strain of Pseudomonas multiresinivorans QL-9a was isolated, and it demonstrated excellent degradation ability against signaling molecules and reduced the severity of bacterial soft rot caused by Pectobacterium carotovorum.
Article
Immunology
Melanie Gonzales, Laure Plener, Jean Armengaud, Nicholas Armstrong, Eric Chabriere, David Daude
Summary: This study demonstrates the importance of Quorum sensing in Burkholderia thailandensis using an enzyme-based quorum quenching strategy. The disruption of quorum sensing greatly affects bacterial behavior, including motility, proteolytic activity, and antimicrobial molecule production. Furthermore, the study shows that quorum sensing disruption significantly enhances the bactericidal activity against fungi and yeast. These findings are of prime interest for understanding the virulence of Burkholderia species and developing alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Flavio Ballante, Maria V. Turkina, Maria Ntzouni, Karl-Eric Magnusson, Elena Vikstrom
Summary: Quorum sensing (QS) is a cell-cell communication system used by bacteria to sense population density and regulate collective behaviors. In this study, four new AHL analogs were designed and evaluated as quorum-sensing inhibitors (QSIs). The compounds were found to reduce the production of signal molecules and certain virulence traits in Pseudomonas aeruginosa.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemical Research Methods
Federica Dal Bello, Michael Zorzi, Riccardo Aigotti, Davide Medica, Vito Fanelli, Vincenzo Cantaluppi, Eleonora Amante, Viviana Teresa Orlandi, Claudio Medana
Summary: This study aimed to develop new analytical methods based on liquid chromatography-mass spectrometry for the detection and quantification of QS signalling molecules in biological samples. Several unknown AHL and HQ compounds were identified and characterized, and the ability of MRM quantitation for virulence factors during sepsis was confirmed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Engineering, Environmental
Xueping Wang, Deshuang Yu, Guanghui Chen, Chengju Liu, Ao Xu, Zhihao Tang
Summary: This paper discusses the bacterial communication mechanism based on QS and the environmental factors affecting QS, compares QS inhibition strategies, and explores the role and prospects of QQ in long-term operation systems.
WATER ENVIRONMENT RESEARCH
(2021)
Article
Neurosciences
Takeshi Morita, Shannan P. McClain, Lyn M. Batia, Maurizio Pellegrino, Sarah R. Wilson, Michael A. Kienzler, Kyle Lyman, Anne Sofie Braun Olsen, Justin F. Wong, Cheryl L. Stucky, Rachel B. Brem, Diana M. Bautista
Article
Biology
Rose Z. Hill, Benjamin U. Hoffman, Takeshi Morita, Stephanie M. Campos, Ellen A. Lumpkin, Rachel B. Brem, Diana M. Bautista
Article
Neurosciences
Sarah R. Wilson, Aislyn M. Nelson, Lyn Batia, Takeshi Morita, Daniel Estandian, David M. Owens, Ellen A. Lumpkin, Diana M. Bautista
JOURNAL OF NEUROSCIENCE
(2013)
Article
Neurosciences
Rose Z. Hill, Takeshi Morita, Rachel B. Brem, Diana M. Bautista
JOURNAL OF NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Takeshi Morita, Heejae Kang, Jason Wolfe, Shantanu P. Jadhav, Daniel E. Feldman
Article
Multidisciplinary Sciences
Kristin A. Gerhold, Maurizio Pellegrino, Makoto Tsunozaki, Takeshi Morita, Duncan B. Leitch, Pamela R. Tsuruda, Rachel B. Brem, Kenneth C. Catania, Diana M. Bautista
Article
Biology
Nipun S. Basrur, Maria Elena De Obaldia, Takeshi Morita, Margaret Herre, Ricarda K. von Heynitz, Yael N. Tsitohay, Leslie B. Vosshall