Article
Cell Biology
Robert C. C. Cail, Cyna R. R. Shirazinejad, David G. G. Drubin
Summary: Cail et al. demonstrate that induced nanoscale membrane curvature can recruit endocytic proteins and produce cargo-laden vesicles without the coat protein clathrin. However, the induced curvature does not bypass AP2 and FCHo1/2 functions. This work suggests that the essential function of clathrin is to physically stabilize the curvature of the evolving vesicle.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Simon Lin, Subramanya Pandruvada, Hong Yu
Summary: Treatment with S1PR2 antagonist or S1PR2 shRNA enhanced osteogenesis in BMSCs via different signaling pathways, including increased vesicle trafficking-associated proteins and activation of Wnt/Ca2+ signaling, BMP/Smad signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Zhenghao Chen, Satoshi Watanabe, Hironori Hashida, Michio Inoue, Yasukazu Daigaku, Masahide Kikkawa, Kenji Inaba
Summary: This study used nanobody/megabody technologies to determine the structures of human SPCA1a in the ATP and Ca2+/Mn2+-bound (E1-ATP) state and the metal-free phosphorylated (E2P) state, revealing the unique mechanisms of SPCA1a-mediated Ca2+/Mn2+ transport.
Article
Multidisciplinary Sciences
Jieyu Wu, Liuyang Wang, John F. Ervin, Shih-Hsiu J. Wang, Erik Soderblom, Dennis Ko, Dong Yan
Summary: Aging is a major risk factor for neurodegenerative diseases, leading to functional decline and degeneration of neurons. Through a new method of neuronal proteomic profiling, certain proteins, including TMC-1, were found to have increased expression levels during aging, and TMC-1 was shown to have an anti-aging function in all tested neurons by regulating GABA signaling. Furthermore, the study demonstrated the vital role of metabotropic GABA receptors and G protein GOA-1 in the anti-neuronal aging functions of TMC-1 and GABA, and the activation of GABA receptors prevents neuronal aging by inhibiting the PLC ss-PKC pathway.
Article
Biochemistry & Molecular Biology
Nghi N. B. Tran, A. T. A. Bui, Valeria Jaramillo-Martinez, Joachim Weber, Qinghai Zhang, Ina L. Urbatsch
Summary: P-glycoprotein (Pgp) is a multidrug transporter that exports hydrophobic compounds out of cells, affecting the bioavailability and pharmacokinetics of drugs. Lipids and cholesterol have been found to modulate the activity of Pgp. However, lipid extracts from different sources are not suitable for biophysical assays. This study investigates how different lipid compositions affect the activity of Pgp and finds that certain lipid mixtures can restore Pgp functionality.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rupam Paul, Sourav Banerjee, Samarpita Sen, Pratiksha Dubey, Saptarshi Maji, Anand K. Bachhawat, Rupak Datta, Arnab Gupta
Summary: Copper is essential for life, but excessive amounts can be toxic. Leishmania parasites have high tolerance to copper stress and employ LmATP7 to survive in host macrophages. The interaction between Leishmania and macrophages involves upregulation of host ATP7A and increased expression of LmATP7. Overexpression of LmATP7 enhances parasite survivability within macrophages.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Ge-Hong Sun-Wada, Yoh Wada
Summary: Vacuolar-type H+-ATPase (V-ATPase) is an electrogenic proton pump that acidifies intracellular organelles and also participates in the acidification of extracellular compartments. It plays important roles in cell biological processes and physiological functions by generating an acidic pH. Acidic endocytic organelles and their trafficking are important for cell polarity maintenance, and V-ATPase is involved in the establishment and maintenance of apico-basal polarity.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Biochemical Research Methods
Anup Gopalakrishna Pillai, Suhita Nadkarni
Summary: This study investigates the relationship between A beta signaling, astrocytic calcium dynamics, and gliotransmission through the development of a biophysical model. The findings suggest that A beta affects the frequency and synchrony of calcium events in astrocytes, leading to a disruption of the temporal correspondence between calcium events and gliotransmitter release.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Clinical Neurology
Tomoya Kubota, Shin Nabatame, Ruka Sato, Manami Hama, Ujiakira Nishiike, Hideki Mochizuki, Masanori P. Takahashi, Takao Takeshima
Summary: Our study identified a FHM2 family with a novel variant in ATP1A2, showing different clinical presentations between the mother and daughter. The mother had a CACNA1A deletion associated with EA2, while her daughter did not. This highlights the importance of genetic analysis in diagnosing neurological ion channel/transporter diseases.
BRAIN & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Susana Sabido-Bozo, Ana Maria Perez-Linero, Javier Manzano-Lopez, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio Lopez, Ralf Erik Wellinger, Manuel Muniz
Summary: This study reveals that the p24 complex promotes the binding of Gea1 with COPI by increasing the association between COPI and the membrane independently of Arf1 activation, and also facilitates the interaction of Arf1 with its COPI effector. Therefore, the p24 complex aids in programming Arf1 activation by Gea1 to selectively assemble COPI coats at the cis-Golgi compartment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Miyako Nakano, Susana Sabido-Bozo, Kouta Okazaki, Auxiliadora Aguilera-Romero, Sofia Rodriguez-Gallardo, Alejandro Cortes-Gomez, Sergio Lopez, Atsuko Ikeda, Kouichi Funato, Manuel Muniz
Summary: The method described in the paper allows for purification and structural analysis of the GPI glycan of individual GPI-APs in yeast, providing insight into the structural remodeling of GPI glycans during transport to the cell surface. This specialized protocol can also be used to investigate the GPI-AP biosynthetic pathway and confirm predicted GPI-anchoring of individual proteins.
Article
Multidisciplinary Sciences
Auxiliadora Aguilera-Romero, Susana Sabido-Bozo, Sergio Lopez, Alejandro Cortes-Gomez, Sofia Rodriguez-Gallardo, Ana Maria Perez-Linero, Isabelle Riezman, Howard Riezman, Manuel Muniz
Summary: This protocol enables the purification and analysis of the lipid moiety of individual GPI-anchored proteins in yeast, aiding in the determination of their precise GPI-lipid structure. It can be adapted to study the lipid composition of GPI anchors in various types of proteins.
Article
Biochemistry & Molecular Biology
Ignacio Flor-Parra, Susana Sabido-Bozo, Atsuko Ikeda, Kazuki Hanaoka, Auxiliadora Aguilera-Romero, Kouichi Funato, Manuel Muniz, Rafael Lucena
Summary: Cell division is triggered when cells reach a certain size, and previous research suggests that growth rate and cell size are linked by ceramide-dependent signals in budding yeast. This study investigated the conservation of growth control mechanisms in fission yeast and found that Lac1, but not Lag1, is essential for regulating cell growth and size by producing specific ceramide and sphingolipid species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Javier Manzano-Lopez, Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Ana Maria Perez-Linero, Rafael Lucena, Antonio Cordones-Romero, Sergio Lopez, Auxiliadora Aguilera-Romero, Manuel Muniz
Summary: Intracellular trafficking depends on transient interactions between cargo proteins and transport machinery. Crosslinking assay can be used to study these interactions.
Article
Biochemistry & Molecular Biology
Mayte Medrano, Ana Lemus-Conejo, Sergio Lopez, Maria C. Millan-Linares, Maria A. Rosillo, Manuel Muniz, Rocio Calderon, Sara Jaramillo, Jose A. Perez-Simon, Francisco J. G. Muriana, Rocio Abia
Summary: Obesity is associated with disruptions in adaptive immune system. This study investigates the effects of dietary fatty acids on T-cell maintenance in bone marrow. The findings reveal a critical role of dietary fatty acids in the selective acquisition of T-cell subsets, particularly the maintenance of CD4(+) T cells by oleic acid.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Cell Biology
Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Atsuko Ikeda, Misako Araki, Kouta Okazaki, Miyako Nakano, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio Lopez, Miho Waga, Akihiko Nakano, Kazuo Kurokawa, Manuel Muniz, Kouichi Funato
Summary: Our study reveals that the presence of C26 ceramide in the endoplasmic reticulum membrane drives clustering and sorting of GPI-APs, and the C26 ceramide is also incorporated into the lipid moiety of GPI-APs. Additionally, the GPI-glycan remodelase Ted1 monitors GPI-APs with a C26 ceramide moiety, which is necessary for receptor-mediated export of GPI-APs.
Editorial Material
Mathematical & Computational Biology
Ralf E. Wellinger, Jesus S. Aguilar-Ruiz
Article
Microbiology
Bazile Ravoityte, Juliana Luksa, Ralf Erik Wellinger, Saulius Serva, Elena Serviene
Summary: This study analyzed the impact of L-A virus on gene expression in yeast hosts using high-throughput RNA sequencing data. It was found that the presence of L-A virus resulted in moderate alterations in gene expression, particularly in genes involved in ribosome biogenesis. Additionally, the transcriptional responses to L-A maintenance were similar to those induced by stress or nutrient availability. The study also identified yeast transcriptional regulators that modulate the levels of L-A dsRNA in cells.
Article
Biology
Raffaele Nicastro, Helene Gaillard, Laura Zarzuela, Marie-Pierre Peli-Gulli, Elisabet Fernandez-Garcia, Mercedes Tome, Nestor Garcia-Rodriguez, Raul Duran, Claudio De Virgilio, Ralf Erik Wellinger
Summary: This research reveals that elevated levels of cytoplasmic manganese stimulate the activity of TORC1 kinase, regulating cell growth and metabolism. Manganese activates TORC1 more efficiently than magnesium and can control autophagy and mitochondrion-to-nucleus retrograde signaling. These findings are significant for understanding the role of manganese in human diseases.
Article
Biochemistry & Molecular Biology
Irene Pazos, Marta Puig-Tinto, Laura Betancur, Jorge Cordero, Nereida Jimenez-Menendez, Marc Abella, Altair C. Hernandez, Ana G. Duran, Emi Adachi-Fernandez, Carla Belmonte-Mateos, Susana Sabido-Bozo, Sebastien Tosi, Akiko Nezu, Baldomero Oliva, Julien Colombelli, Todd R. Graham, Tamotsu Yoshimori, Manuel Muniz, Maho Hamasaki, Oriol Gallego
Summary: Multisubunit Tethering Complexes (MTCs) are conserved protein complexes that tether vesicles at the acceptor membrane. P4-ATPases, a family of lipid flippases, interact with MTCs involved in Golgi transport. The interaction between TRAPPIII and P4-ATPase Drs2 is required for Atg9 delivery during selective autophagy, independent of the canonical functions of P4-ATPase. The I(S/R)TTK motif mediates the interaction of P4-ATPases with MTCs, implying a broader role of P4-ATPases in MTC regulation.
Editorial Material
Cell Biology
Auxiliadora Aguilera-Romero, Manuel Muniz
Summary: This article previews the work from the Kinoshita lab, which demonstrates the collaboration between specific GPI anchor signal sequences and ER-resident protein ARV1 in upregulating GPI biosynthesis. GPI anchoring is a crucial post-translational modification in eukaryotes that links proteins to the plasma membrane. Liu et al. (2023. J. Cell Biol.https://doi.org/10.1083/jcb.202208159) suggest, for the first time, a regulatory mechanism for the on-demand biosynthesis of GPI glycolipid precursors.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ines Garcia De Oya, Javier Manzano-Lopez, Alejandra Alvarez-llamas, Maria de la Paz Vazquez-Aroca, Cristina Cepeda-Garcia, Fernando Monje-Casas
Summary: Both the spindle microtubule-organizing centers and the nuclear pore complexes collaborate in regulating essential processes during cell division, despite their distinct molecular conformation and overall functions. A new link has been established between these two structures in budding yeast, showing an interaction between the Bfa1/Bub2 complex and the Nup159 nucleoporin. This interaction is stimulated during anaphase and assists in the Nup159-dependent autophagy pathway.
