Article
Multidisciplinary Sciences
Michael F. Fuss, Jan-Philip Wieferig, Robin A. Corey, Yvonne Hellmich, Igor Tascon, Joana S. Sousa, Phillip J. Stansfeld, Janet Vonck, Inga Haenelt
Summary: Cyclic di-AMP inhibits the potassium transporter KimA by reducing the mobility of transmembrane helices at the cytosolic side of the K+ binding site, trapping KimA in an inward-occluded conformation. This study provides insights into how the second messenger cyclic di-AMP regulates bacterial physiology.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Daniel A. Pensinger, Kimberly V. Gutierrez, Hans B. Smith, William J. B. Vincent, David S. Stevenson, Katherine A. Black, Krizia M. Perez-Medina, Joseph P. Dillard, Kyu Y. Rhee, Daniel Amador-Noguez, TuAnh N. Huynh, John-Demian Sauer
Summary: Bacterial pathogens, such as Listeria monocytogenes, need to adapt to the host cytosol environment in order to survive and cause disease. GlmR, a protein of unknown function, is essential for the survival and virulence of Listeria monocytogenes. Understanding the role of GlmR and how cytosolic pathogens adapt and survive is crucial for developing new treatments.
Article
Infectious Diseases
Pavel A. Andriyanov, Pavel A. Zhurilov, Elena A. Liskova, Tatyana I. Karpova, Elena V. Sokolova, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Olga L. Voronina, Ekaterina K. Psareva, Igor S. Tartakovsky, Denis V. Kolbasov, Svetlana A. Ermolaeva
Summary: Susceptibility testing of 117 L. monocytogenes strains to 23 antibiotics revealed a varied response, with most strains showing sensitivity to multiple antibiotics but some strains exhibiting resistance to specific drugs. There was a shift in the proportion of resistant strains over time, with different antibiotics showing unique patterns of resistance evolution.
Article
Food Science & Technology
Ying Feng, Zhangkai Cheng, Xianhu Wei, Moutong Chen, Jumei Zhang, Youxiong Zhang, Liang Xue, Minling Chen, Fan Li, Yuting Shang, Tingting Liang, Yu Ding, Qingping Wu
Summary: In this study, a novel method that combines metabolomics with deep learning was developed for the detection and identification of Listeria monocytogenes. By establishing a convolutional neural network model and using a pseudotargeted metabolomics approach, high prediction accuracies were achieved and different Listeria species were successfully distinguished. This is a promising powerful tool for the detection and classification of pathogens.
Article
Microbiology
Aaron S. Dhanda, A. Wayne Vogl, Fern Ness, Metello Innocenti, Julian A. Guttman
Summary: Direct cell-to-cell spreading of Listeria monocytogenes requires the induction of actin-based membrane protrusions in donor cells, which are endocytosed by adjacent receiving cells. Formin mDia1 plays a vital role in the assembly of the F-actin shell during this process, leading to efficient cell-to-cell transfer of the bacteria.
Article
Biochemistry & Molecular Biology
Anne-Sophie Hascoet, Carolina Ripolles-Avila, Brayan R. H. Cervantes-Huaman, Jose Juan Rodriguez-Jerez
Summary: Listeria monocytogenes remains a significant public health challenge in the meat sector, and utilizing Bacillus safensis from resident microbiota as a potential inhibitor could be an effective strategy for controlling the pathogen. The preformed biofilms of Bacillus safensis have shown to inhibit adhesion and biofilm formation of L. monocytogenes strains, providing a new approach to combat the persistence of the pathogen in meat industry surfaces.
Article
Microbiology
Hasan Tukenmez, Pardeep Singh, Souvik Sarkar, Melike Cakir, Ana H. Oliveira, Cecilia Lindgren, Karolis Vaitkevicius, Mari Bonde, A. Elisabeth Sauer-Eriksson, Fredrik Almqvist, Jorgen Johansson
Summary: Bacteria resistant to antibiotics are a major problem, requiring the development of new antimicrobial drugs. In this study, the effectiveness of a new compound, PS900, in inhibiting virulence factor expression in Listeria monocytogenes was demonstrated. Furthermore, PS900 was found to potentiate the bactericidal effects of cholic acid and osmolytes, making it a promising candidate for future antibacterial drug design.
Article
Food Science & Technology
Ekaterina K. Psareva, Elena A. Liskova, Irina Razheva, Yulia K. Yushina, Maria A. Grudistova, Nadezda A. Gladkova, Eugene A. Potemkin, Pavel A. Zhurilov, Elena Sokolova, Pavel A. Andriyanov, Olga L. Voronina, Denis Kolbasov, Svetlana A. Ermolaeva
Summary: The study identified high molecular diversity among Listeria monocytogenes strains isolated in Central European Russia, with significant differences observed between strains of different lineages. The closure of the Russian market for imported food in 2019-2020 had a notable impact on the types and distribution of isolated strains.
Article
Food Science & Technology
Brianmax A. Takundwa, Prashant Bhagwat, Santhosh Pillai, Oluwatosin A. Ijabadeniyi
Summary: The combination of nisin, oregano, and ultrasound was found to be an effective alternative to chemical treatments for reducing E. coli and L. monocytogenes on lettuce, while maintaining its appearance and quality. Furthermore, the treated lettuce samples showed significant reductions in bacterial counts without compromising textural properties. This innovative approach offers a promising solution for enhancing food safety in the fresh produce industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mingyuan Tang, Sicheng Tian, Kehan Chen, Qiuyang Zhang, Yao Lei, Tian Tang, Jumei Zeng, Chuan Wang
Summary: This study found that membrane vesicles derived from Listeria monocytogenes have good safety and effective immunogenicity, and may serve as potential antigen and drug delivery vectors.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Analytical
Xiaohua Jiang, Zhiwen Lv, Changquan Rao, Xiaowen Chen, Ying Zhang, Feng Lin
Summary: Researchers have developed a simple and highly sensitive nanozyme-based electrochemical sensor for the detection of Listeria monocytogenes (LM). By preparing Pt nanoparticles/hollow carbon spheres as the nanozyme, and using aptamers to regulate its activity, the sensor showed excellent sensing performances for LM.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biotechnology & Applied Microbiology
Jillian Rumore, Matthew Walker, Franco Pagotto, Jessica D. Forbes, Christy-Lynn Peterson, Andrea D. Tyler, Morag Graham, Gary Van Domselaar, Celine Nadon, Aleisha Reimer, Natalie Knox
Summary: This study evaluated the performance of a foodborne pathogen-specific reference database compared to a universal reference database, and found that using a pathogen-specific database can achieve higher classification precision in complex food matrices, with lower computational resource requirements.
Article
Microbiology
Federica D'Onofrio, Maria Schirone, Ivanka Krasteva, Manuela Tittarelli, Luigi Iannetti, Francesco Pomilio, Marina Torresi, Antonello Paparella, Nicola D'Alterio, Mirella Luciani
Summary: This study investigated the protein expression patterns of L. monocytogenes strains under stressful conditions and revealed differences in protein expression related to stress response, virulence, and pathogenesis. The findings provide valuable information about the adaptation and potential treatments of this pathogen, highlighting the importance of studying proteomic profiles and utilizing bioinformatics for pathogen analysis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Horticulture
Ke Sarengaowa, Ke Feng, Yuanzheng Li, Ya Long, Wenzhong Hu
Summary: This study evaluated the effects of an alginate-based edible coating containing thyme essential oil (AEC-TEO) on the quality and safety of fresh-cut potatoes during a storage period of 16 days at 4 degrees C. The results showed that treatment with AEC-TEO at a 0.05% concentration was the most beneficial for maintaining quality and inhibiting the microorganism growth of fresh-cut potatoes. The reductions in bacterial total counts, total coliform counts, yeast and mold counts, and Lactobacillus counts were 2.41 log cfu/g, 1.37 log cfu/g, 1.21 log cfu/g, and 2 log cfu/g, and Listeria monocytogenes decreased by 3.63 log cfu/g on fresh-cut potatoes after 16 days. Therefore, AEC-TEO effectively improved the quality of fresh-cut potatoes and prolonged their shelf life to a certain extent.
Article
Microbiology
Silvia Scattolini, Daniela D'Angelantonio, Arianna Boni, Iolanda Mangone, Maurilia Marcacci, Noemi Battistelli, Krizia D'Agostino, Francesco Pomilio, Cesare Camma, Giacomo Migliorati, Giuseppe Aprea
Summary: Listeria monocytogenes is a pathogenic bacterium responsible for listeriosis, which can be transmitted through contaminated ready-to-eat foods. Phages like phi IZSAM-1 have shown potential as biodecontaminants for drug-resistant Listeria monocytogenes strains, demonstrating effective control in vitro experiments. This study highlights the importance of phage isolation from the same environments as the target pathogens for successful biocontrol applications in food production.
Article
Microbiology
Jeanine Rismondo, Lisa M. Schulz, Maria Yacoub, Ashima Wadhawan, Michael Hoppert, Marc S. Dionne, Angelika Gruendling
Summary: The ABC transporter EslABC is associated with the intrinsic lysozyme resistance of Listeria monocytogenes. The deletion of EslB not only affects lysozyme resistance, but also influences endogenous cell lysis, cell wall biosynthesis, cell division, and growth in media containing high concentrations of sugars. EslB is an important determinant for cell wall integrity in L. monocytogenes through a yet-unknown mechanism.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Bjoern Richts, Fabian M. Commichau
Summary: Vitamin B6, essential for animals and humans, cannot be synthesized and must be obtained through diet. Recent studies have identified new pathways for enhancing bacterial production of vitamin B6 through genetic engineering.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Microbiology
Robert Hertel, Johannes Gibhardt, Marion Martienssen, Ramona Kuhn, Fabian M. Commichau
Summary: Glyphosate is a nonselective herbicide widely used in agriculture for killing weeds and competing plants. Although considered safe for animals and humans, its intensive use can lead to environmental issues, such as negative impacts on biodiversity. Bacteria can evolve resistance to glyphosate through various mechanisms, highlighting their adaptability to anthropogenic substances.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Sabrina Wamp, Patricia Rothe, Daniel Stern, Gudrun Holland, Janina Doehling, Sven Halbedel
Summary: This study discovered a new signaling route that controls PG production in the human pathogen Listeria monocytogenes and its role in intrinsic resistance to beta-lactam antibiotics. This signaling involves the PrkA kinase and its substrate ReoM, which regulates PG biosynthesis by controlling the stability of MurA. The transglycosylase RodA and the transpeptidase PBP B3 are also important downstream factors. These findings shed new light on the regulation of PG biosynthesis and antibiotic resistance in Gram-positive bacteria.
Article
Immunology
Raskit Lachmann, Sven Halbedel, Stefanie Lueth, Alexandra Holzer, Marlen Adler, Ariane Pietzka, Sascha Al Dahouk, Klaus Stark, Antje Flieger, Sylvia Kleta, Hendrik Wilking
Summary: Invasive listeriosis outbreaks associated with the consumption of smoked and graved salmon products were investigated in Germany. Whole-genome sequencing and patient interviews were used to identify and analyze the outbreaks. The study identified 22 independent outbreaks between 2010 and 2021, with 228 cases and 50 deaths in Germany. Cross-border outbreaks were also observed. The findings highlight the serious risk posed by L. monocytogenes-contaminated salmon products in Germany, emphasizing the need for improved hygiene measures and better awareness among susceptible individuals.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Mengyi Wang, Sabrina Wamp, Johannes Gibhardt, Gudrun Holland, Inge Schwedt, Kai-Uwe Schmidtke, Katrin Scheibner, Sven Halbedel, Fabian M. Commichau
Summary: This study investigates the phenotypes of mutants with defects in c-di-AMP metabolism in Listeria monocytogenes and identifies suppressor mutants that restore their growth defects. The results show that c-di-AMP plays a key role in controlling osmolyte homeostasis in L. monocytogenes, and that the Opp oligopeptide transport system is involved in the uptake of the antibiotic fosfomycin. These findings contribute to our understanding of the complex metabolic regulation in L. monocytogenes.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martin A. Fischer, Tim Engelgeh, Patricia Rothe, Stephan Fuchs, Andrea Thuermer, Sven Halbedel
Summary: Listeria monocytogenes, a Gram-positive bacterium, is widespread in the environment and infects humans via contaminated food, posing a particularly dangerous threat to at-risk patients. By using Tn-Seq technology, crucial cellular processes and genes necessary for pathogen growth both inside and outside the host have been identified, offering potential novel options for developing antilisterial drugs.
Article
Chemistry, Medicinal
Ann-Britt Schafer, Maurice Steenhuis, Kin Ki Jim, Jolanda Neef, Sarah O'Keefe, Roger C. Whitehead, Eileithyia Swanton, Biwen Wang, Sven Halbedel, Stephen High, Jan Maarten van Dijl, Joen Luirink, Michaela Wenzel
Summary: Eeyarestatin 24 (ES24) is a promising new antibiotic that inhibits SecYEG-mediated protein transport in Gram-negative bacteria and has notable activity against Gram-positive bacteria. It causes DNA damage and membrane potential dissipation in Escherichia coli but not in Bacillus subtilis. ES24 differs from nitrofurantoin in its mechanism of action and outperforms it in a zebrafish embryo pneumococcal infection model, suggesting multiple targets in bacteria.
ACS INFECTIOUS DISEASES
(2023)
Article
Environmental Sciences
Inge Schwedt, Madeline Collignon, Carolin Mittelstaedt, Florian Giudici, Johanna Rapp, Janek Meissner, Hannes Link, Robert Hertel, Fabian M. Commichau
Summary: Glyphosate inhibits EPSP synthase in bacteria and other organisms, causing depletion of aromatic amino acids, folate, and quinones. The Burkholderia anthina DSM 16086 strain develops resistance to glyphosate by acquiring mutations in the ppsR gene, resulting in increased cellular PEP concentration that prevents EPSP synthase inhibition. Overexpression of the E. coli ppsA gene did not confer glyphosate resistance in B. subtilis and E. coli, suggesting that the mutational inactivation of ppsR leading to PpsA overactivity is a unique mechanism in B. anthina.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Microbiology
Sven Halbedel, Ida Sperle, Raskit Lachmann, Sylvia Kleta, Martin A. Fischer, Sabrina Wamp, Alexandra Holzer, Stefanie Lueth, Larissa Murr, Christin Freitag, Laura Espenhain, Roger Stephan, Ariane Pietzka, Susanne Schjorring, Guido Bloemberg, Mareike Wenning, Sascha Al Dahouk, Hendrik Wilking, Antje Flieger
Summary: The use of whole-genome sequencing (WGS) has allowed for the identification of a multinational listeriosis outbreak caused by Listeria monocytogenes. The outbreak was traced back to smoked rainbow trout filets from a Danish producer, highlighting the potential for international food trade to contribute to the spread of infectious diseases. This study emphasizes the importance of surveillance and collaboration in preventing further outbreaks of listeriosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jeanine Rismondo, Cornelia Grosse, Dietrich H. Nies
Summary: The combined actions of an inner membrane with a transenvelope efflux system play an important role in the efficient removal of essential-but-toxic transition metal cations in bacteria.
MICROBIOLOGY SPECTRUM
(2023)
Article
Environmental Sciences
Lisa Maria Schulz, Alicia Konrath, Jeanine Rismondo
Summary: Listeria monocytogenes is a foodborne pathogen that can grow in various environmental conditions. In this study, two wildtype strains of L. monocytogenes were examined for their ability to grow under salt and pH stress in Listeria synthetic medium (LSM). It was found that a wide range of carbon sources could support the growth of these strains in LSM, but hexose phosphate sugars required pre-growth under conditions where the virulence regulator PrfA was active. Additionally, the amino acid sugar N-acetylmannosamine (ManNAc) promoted the growth of both strains in LSM, with specific genes in the lmo2795-nanE operon playing a role in this process.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Microbiology
Lars Lilge, Robert Hertel, Kambiz Morabbi Heravi, Marius Henkel, Fabian M. Commichau, Rudolf Hausmann
Summary: The Bacillus subtilis subsp. subtilis type strain DSM10 has been utilized as a reference in various studies, but its genome information was previously lacking. Therefore, a whole-genome sequencing was conducted and compared with the related B. subtilis strain NCIB3610.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)