期刊
ANALYST
卷 140, 期 15, 页码 4997-5002出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an00737b
关键词
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资金
- European Union under the European Regional Development Fund (JCET-UJ) [POIG.01.01.02-00-069/09]
- National Science Center [DEC-2013/09/N/NZ7/00626]
In this work we apply FT-IR imaging of large areas of liver tissue cross-section samples (similar to 5 cm x 5 cm) for quantitative assessment of steatosis in murine model of Non-Alcoholic Fatty Liver (NAFLD). We quantified the area of liver tissue occupied by lipid droplets (LDs) by FT-IR imaging and Oil Red O (ORO) staining for comparison. Two alternative FT-IR based approaches are presented. The first, straightforward method, was based on average spectra from tissues and provided values of the fat content by using a PLS regression model and the reference method. The second one - the chemometric-based method - enabled us to determine the values of the fat content, independently of the reference method by means of k-means cluster (KMC) analysis. In summary, FT-IR images of large size liver sections may prove to be useful for quantifying liver steatosis without the need of tissue staining.
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